Garuda - Garba Rujukan Digital

Article Per Year (5 Year)

All

HAYATI Journal of Biosciences

hayati
Website

Published by Institut Pertanian Bogor

ISSN : 19783019 EISSN : 20864094 DOI : -

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

HAYATI Journal of Biosciences (HAYATI J Biosci) publishes articles and short communication in tropical bioscience fields such as development, biotechnology, biodiversity and environmental issues. HAYATI J Biosci covers wide range of all life forms topics including virus, microbes, fungi, plants, animal and human. HAYATI J Biosci has been also indexed/registered in Crossref, DOAJ, CABI, EBSCO, Agricola and ProQuest.

Arjuna Subject : -

Articles 9 Documents

Search results for , issue "Vol. 22 No. 4 (2015): October 2015" : 9 Documents clear

Identification of Diagnostic Mitochondrial DNA Single Nucleotide Polymorphisms Specific to Sumatran Orangutan (Pongo abelii) Populations Puji Rianti; Dyah Perwitasari-Farajallah; Dondin Sajuthi; Joko Pamungkas; Alexander Nater; Michael Krützen
HAYATI Journal of Biosciences Vol. 22 No. 4 (2015): October 2015
Publisher : Bogor Agricultural University, Indonesia

The hypervariable region I of mitochondrial DNA has frequently been used to distinguish among populations, in particular in species with strong female philopatry. In such cases, populations are expected to diverge rapidly for hypervariable region I markers because of the smaller effective population size and thus increased genetic drift. This rapid divergence leads to the accumulation of mutations exclusively found in one population, which may serve as diagnostic single nucleotide polymorphisms (SNPs). To date, diagnostic SNPs distinctive to Sumatran orangutan populations have not yet been described. However, given the continuously declining numbers of Sumatran orangutans, this information can be vital for effective conservation measures, especially regarding reintroductions of orangutans in rehabilitation centers. Phylogenetic analyses of 54 samples of Sumatran orangutans from nine sampling sites with good provenance, we found five major clades and a total of 20 haplotypes. We propose a total of 52 diagnostic SNPs that are specific to Sumatran orangutan populations. Data can be used to develop restriction fragment length polymorphism assays to carry out genetic assignments using basic laboratory equipment to assign Sumatran orangutan to their population of origin.

Impact of Domestication on the Endophytic Fungal Diversity Associated With Wild Zingiberaceae at Mount Halimun Salak National Park Ivan Permana Putra; Gayuh Rahayu; Iman Hidayat
HAYATI Journal of Biosciences Vol. 22 No. 4 (2015): October 2015
Publisher : Bogor Agricultural University, Indonesia

Mount Halimun Salak National Park is one of the tropical forest remnants in Java island. The national park has been recognized with high diversity of wild Zingiberaceae. Of that Zingiberaceae, two species namely Alpinia malaccensis (AM) and Horstendia conica (HC), were domesticated as garden plants in the surrounding area of the forest for medicinal use. The impact of domestication on the fungal endophytes associated with these two species of Zingiberaceae is reported here. Fungal endophyte diversity in the wild and domesticated AM and HC was analyzed based on the culturable fungi. Identification of species level used morphological and molecular approaches of ITS rDNA sequence. This study determined 19 species of fungal endophytes, namely Arthrinium malaysianum, Aspergillus flavipes, As. sydowii, Chaetomium globosum, Cladosporium oxysporum, Cladosporium sp., Colletotrichum boninense-complex, Co. cliviae-complex, Co. gloeosporioides-complex, Diaporthe sp., D. anacardii, D. gardenia, Exophiala sp., E. lecanii-corni, Guignardia mangiferae, Ochroconis gallopava, Penicillium citrinum, Pyricularia costina, and unsporulated Sydowiellaceae. Among them, A. malaysianum, C. globosum, Co. cliviae-complex, D. gardenia, and unsporulated Sydowiellaceae were only found in domesticated plants, while some others were absent. Colletotrichum boninense-complex was commonly found in both wild and domesticated plants. Domestication activity affected the diversity of endophytic fungi of AM and HC.

Encapsulated Synbiotic Dietary Supplementation at Different Dosages to Prevent Vibriosis in White Shrimp, Litopenaeus vannamei Anis Zubaidah; Munti Yuhana; . Widanarni
HAYATI Journal of Biosciences Vol. 22 No. 4 (2015): October 2015
Publisher : Bogor Agricultural University, Indonesia

The aim of this study was to evaluate the effect of encapsulated synbiotic (Bacillus sp. NP5 and oligosaccharide) dietary at different dosages on growth performance, survival rate, feed conversion ratio, and immune responses of Litopenaeus vannamei against Vibrio infection. The shrimps of the main treatments were fed by the diet that contained three different dosages of encapsulated synbiotic [0.5% (A), 1% (B), and 2% (C) (w/w)] with feeding rate of 5% of shrimp biomass (4 times a day). The shrimps of two control treatments (negative control and positive control) were fed only by commercial feed without supplementation of encapsulated synbiotic. The growth, feed conversion ratio, and survival rate were observed after 30 days of encapsulated synbiotic dietary. The shrimps were then challenged by injection of Vibrio harveyi (6 log colony forming units/mL) 0.1 mL/shrimp, excluded the negative control treatment. Afterward, the survival and immune responses were observed for 9 days after experimental infection. The shrimps treated with 2% encapsulated synbiotic (treatment C) in the diet showed the highest growth performance (2.98 ± 0.42%), feed conversion ratio (1.26 ± 0.19), and better immune responses i.e. total hemocyte counts, differential hemocyte count, phenoloxidase, and intestine bacteria observation compared to those of positive control treatment.

Growth and Survival Rate of Tilapia (Oreochromis niloticus) Larvae Fed by Daphnia magna Cultured With Organic Fertilizer Resulted From Probiotic Bacteria Fermentation Vivi Endar Herawati; Johannes Hutabarat; . Pinandoyo; Ocky Karna Radjasa
HAYATI Journal of Biosciences Vol. 22 No. 4 (2015): October 2015
Publisher : Bogor Agricultural University, Indonesia

Daphnia magna is a potential feed for fish. The aim of this research was to find the best treatment and effect of D. magna culture addition from fermented organic fertilizer, to growth and survival rate of Oreochromis niloticus larvae. There were five treatments, each with three repetitions used in the study. All treatments used chicken dung, and different combinations of rice bran, coconut oilcake waste and tilapia larvae. Feeding on tilapia was given by ad libitum method for five times a day until 14 days. Water quality during the research was maintained at temperature 28–29°C, DO 0.3 ppm and pH 8.1–8.2. Observed variables include relative growth rate, survival rate, food consumption rate and water quality. Our results showed that D. magna cultured by fermented organic fertilizer for tilapia larvae (O. niloticus) had high significant effect (p < 0.01) on the relative growth rate and survival rate. Treatment of D. magna cultured by 1.2 g/L chicken manure, 0.9 g/L rice bran and 0.3 g/L coconut oilcake showed the highest value on the relative growth rate (10.86%); survival rate (98.46%) and food consumption at first week (106.43%) and second week (152.76%).

Optimization of Culture Conditions for Maximal Lovastatin Production by Aspergillus terreus (KM017963) under Solid State Fermentation Praveen Vadakke Kamath; Bhargavi Santebennur Dwarakanath; Avinash Chaudhary; Savitha Janakiraman
HAYATI Journal of Biosciences Vol. 22 No. 4 (2015): October 2015
Publisher : Bogor Agricultural University, Indonesia

Effects of various culture conditions on lovastatin production were investigated in Aspergillus terreus (KM017963) grown under solid state fermentation with wheat bran. Lovastatin production was influenced by various physical factors such as pH, temperature, and nutritional factors such as carbon, nitrogen, metal ions/salts etc. Our study established that an initial pH of 6.0, growth temperature of 28 °C–30 °C, inoculum size of 108 spores/mL as the optimal physiological culture conditions for maximal production of lovastatin by A. terreus (KM017963). The carbon sources, glucose or dextrin when supplemented at 3% (w/w) enhanced lovastatin production by five fold when supplemented as individual component in wheat bran. Addition of metal salts such as CuSO4 (8%), FeSO4 (8%), CaCl2 (10%), NaCl (6%) and MgSO4 (6%) enhanced the production by five fold. Supplementation with nitrogen sources, amino acids, hydrocarbons, surfactants and amino acids did not have any profound effect on lovastatin production.

Putative DNA-dependent RNA polymerase in Mitochondrial Plasmid of Paramecium caudatum Stock GT704 Trina Ekawati Tallei
HAYATI Journal of Biosciences Vol. 22 No. 4 (2015): October 2015
Publisher : Bogor Agricultural University, Indonesia

Mitochondria of Paramecium caudatum stock GT704 has a set of four kinds of linear plasmids with sizes of 8.2, 4.1, 2.8 and 1.4 kb. The plasmids of 8.2 and 2.8 kb exist as dimers consisting of 4.1- and 1.4-kb monomers, respectively. The plasmid 2.8 kb, designated as pGT704-2.8, contains an open reading frame encodes for putative DNA-dependent RNA polymerase (RNAP). This study reveals that this RNAP belongs to superfamily of DNA/RNA polymerase and family of T7/T3 single chain RNA polymerase and those of mitochondrial plasmid of fungi belonging to Basidiomycota and Ascomycota. It is suggested that RNAP of pGT704-2.8 can perform transcription without transcription factor as promoter recognition. Given that only two motifs were found, it could not be ascertained whether this RNAP has a full function independently or integrated with mtDNA in carrying out its function.

Antibacterial, Antifungal and Anticancer Activity of Five Strains of Soil Microorganisms Isolated From Tangkuban Perahu Mountain by Fermentation Desak Gede Sri Andayani; Ukan Sukandar; Elin Y. Sukandar; I. Ketut Adnyana
HAYATI Journal of Biosciences Vol. 22 No. 4 (2015): October 2015
Publisher : Bogor Agricultural University, Indonesia

Microorganisms were isolated from soil taken from Tangkuban Perahu mountain. Five strains were investigated in this study, designated TP1, TP2, TP3, TP4, and TP5, respectively. Morphological, biochemical and molecular identifications were conducted for all five strains. These isolates were shown to be closely related to Nocardia sp. YIM 65630 (90%), Streptomyces galbus (99%), Aspergillus unguis (86%), Paecilomyces marquandii (100%) and Nocardia niigatensis (95%), respectively. Production of antibacterial, antifungal and anticancer metabolites was done by fermentation. Screening for bioactivity of five isolates was done by testing the fermentation broth against resistant and pathogenic bacteria, fungi and T47D breast cancer cell line. TP2 strain showed the best bioactivity; the metabolite was purified by extraction with ethyl acetate. Antibacterial, antifungal and anticancer activities from the ethyl acetate extract of TP2 strain were tested by agar diffusion, microdilution and MTT. The extract was shown to be active against methicillin resistant Staphylococcus, methicillin sensitive Staphylococcus aureus, methicillin resistant coagulase negative Staphylococcus, vancomycin resistant Enterococcus, Escherichia coli, Microsforum gypseum with the minimum inhibitory concentration (μg/mL) and diameter of inhibition (mm): 150, 35; 150, 30; 300, 35; 300, 35; 300, 29; 4.7, 36, respectively. The IC50 value of the T47D cell line was 457 μg/mL.

Expression Study of LeGAPDH, LeACO1, LeACS1A, and LeACS2 in Tomato Fruit (Solanum lycopersicum) Pijar Riza Anugerah; Fenny Dwivany; Rizkita Rahmi Esyanti
HAYATI Journal of Biosciences Vol. 22 No. 4 (2015): October 2015
Publisher : Bogor Agricultural University, Indonesia

Tomato is a climacteric fruit, which is characterized by ripening-related increase of respiration and elevated ethylene synthesis. Ethylene is the key hormone in ripening process of climacteric fruits. The objective of this research is to study the expression of three ethylene synthesis genes: LeACO1, LeACS1A, LeACS2, and a housekeeping gene LeGAPDH in ripening tomato fruit. Specific primers have been designed to amplify complementary DNA fragment of LeGAPDH (143 bp), LeACO1 (240 bp), LeACS1A (169 bp), and LeACS2 (148 bp) using polymerase chain reaction. Nucleotide BLAST results of the complementary DNA fragments show high similarity with LeGAPDH (NM_001247874.1), LeACO1 (NM_001247095.1), LeACS1A(NM_001246993.1), LeACS2 (NM_001247249.1), respectively. Expression study showed that LeACO1, LeACS1A, LeACS2, and LeGAPDH genes were expressed in ripening tomato fruit. Isolation methods, reference sequences, and primers used in this study can be used in future experiments to study expression of genes responsible for ethylene synthesis using quantitative polymerase chain reaction and to design better strategy for controlling fruit ripening in agroindustry.

Characterization and Identification of Cellulolytic Bacteria from gut of Worker Macrotermes gilvus Ferbiyanto, Andri; Rusmana, Iman; Raffiudin, Rika
HAYATI Journal of Biosciences Vol. 22 No. 4 (2015): October 2015
Publisher : Bogor Agricultural University, Indonesia

As a social insect, termite colony consists of three castes, i.e. reproductive, soldier, and worker castes. In their role of cellulose digestion, the worker termites use two sources of cellulolytic enzyme that include cellulases produced by the termite and the gut symbions. Macrotermes gilvus classified in mound builder termite, mostly depend on cellulolytic bacteria for cellulose digestion. This study aims to characterize cellulolytic bacteria of termite gut symbionts of worker M. gilvus and to identify the cellulolytic bacteria based on sequences of 16S ribosomal RNA (rRNA) gene. Cellulolytic bacteria of termite gut were isolated and cultured in CMC (Carboxymethyl cellulose) media. The biochemical characters of bacterial isolates were assayed using Microbact 12A and 12B. Cellulolytic activity was determined based on formation of clear zone and cellulolytic index on CMC plate media. The bacterial isolate that has the highest cellulolytic index was analyzed for its 16S rRNA gene sequences. Four isolates of cellulolytic bacteria were successfully isolated from gut of M. gilvus with aerobic and anaerobic conditions. The highest formation of cellulolytic index (2.5) was revealed by RA2. BLAST-N (Basic Local Alignment Search Tool for Nucleotides) result of 16S rRNA gene sequences of RU4 and RA2 isolates showed that the isolate has similarity with Bacillus megaterium and Paracoccus yeei, respectively. This result indicated that RA2 isolate was P. yeei, a cellulolytic bacterium of a termite gut of M. gilvus.

Page 1 of 1 | Total Record : 9

Filter by Year

2015 2015

Filter By Issues

All Issue Vol. 33 No. 2 (2026): March 2026 Vol. 33 No. 1 (2026): January 2026 Vol. 32 No. 6 (2025): November 2025 Vol. 32 No. 5 (2025): September 2025 Vol. 32 No. 4 (2025): July 2025 Vol. 32 No. 3 (2025): May 2025 Vol. 32 No. 2 (2025): March 2025 Vol. 32 No. 1 (2025): January 2025 Vol. 31 No. 6 (2024): November 2024 Vol. 31 No. 5 (2024): September 2024 Vol. 31 No. 4 (2024): July 2024 Vol. 31 No. 3 (2024): May 2024 Vol. 31 No. 2 (2024): March 2024 Vol. 31 No. 1 (2024): January 2024 Vol. 30 No. 6 (2023): November 2023 Vol. 30 No. 5 (2023): September 2023 Vol. 30 No. 4 (2023): July 2023 Vol. 30 No. 3 (2023): May 2023 Vol. 30 No. 2 (2023): March 2023 Vol. 30 No. 1 (2023): January 2023 Vol. 29 No. 6 (2022): November 2022 Vol. 29 No. 5 (2022): September 2022 Vol. 29 No. 4 (2022): July 2022 Vol. 29 No. 3 (2022): May 2022 Vol. 29 No. 2 (2022): March 2022 Vol. 29 No. 1 (2022): January 2022 Vol. 28 No. 4 (2021): October 2021 Vol. 28 No. 3 (2021): July 2021 Vol. 28 No. 2 (2021): April 2021 Vol. 28 No. 1 (2021): January 2021 Vol. 27 No. 4 (2020): October 2020 Vol. 27 No. 3 (2020): July 2020 Vol. 27 No. 2 (2020): April 2020 Vol. 27 No. 1 (2020): January 2020 Vol. 26 No. 4 (2019): October 2019 Vol. 26 No. 3 (2019): July 2019 Vol. 26 No. 2 (2019): April 2019 Vol. 26 No. 1 (2019): January 2019 Vol. 25 No. 4 (2018): October 2018 Vol. 25 No. 3 (2018): July 2018 Vol. 25 No. 2 (2018): April 2018 Vol. 25 No. 1 (2018): January 2018 Vol. 24 No. 4 (2017): October 2017 Vol. 24 No. 3 (2017): July 2017 Vol. 24 No. 2 (2017): April 2017 Vol. 24 No. 1 (2017): January 2017 Vol. 23 No. 4 (2016): October 2016 Vol. 23 No. 3 (2016): July 2016 Vol. 23 No. 2 (2016): April 2016 Vol. 23 No. 1 (2016): January 2016 Vol. 22 No. 4 (2015): October 2015 Vol. 22 No. 3 (2015): July 2015 Vol. 22 No. 2 (2015): April 2015 Vol. 22 No. 1 (2015): January 2015 Vol. 21 No. 4 (2014): December 2014 Vol. 21 No. 3 (2014): September 2014 Vol. 21 No. 2 (2014): June 2014 Vol. 21 No. 1 (2014): March 2014 Vol. 20 No. 4 (2013): December 2013 Vol. 20 No. 3 (2013): September 2013 Vol. 20 No. 2 (2013): June 2013 Vol. 20 No. 1 (2013): March 2013 Vol. 19 No. 4 (2012): December 2012 Vol. 19 No. 3 (2012): September 2012 Vol. 19 No. 2 (2012): June 2012 Vol. 19 No. 1 (2012): March 2012 Vol. 18 No. 4 (2011): December 2011 Vol. 18 No. 3 (2011): September 2011 Vol. 18 No. 2 (2011): June 2011 Vol. 18 No. 1 (2011): March 2011 Vol. 17 No. 4 (2010): December 2010 Vol. 17 No. 3 (2010): September 2010 Vol. 17 No. 2 (2010): June 2010 Vol. 17 No. 1 (2010): March 2010 Vol. 16 No. 4 (2009): December 2009 Vol. 16 No. 3 (2009): September 2009 Vol. 16 No. 2 (2009): June 2009 Vol. 16 No. 1 (2009): March 2009 Vol. 15 No. 4 (2008): December 2008 Vol. 15 No. 3 (2008): September 2008 Vol. 15 No. 2 (2008): June 2008 Vol. 15 No. 1 (2008): March 2008 Vol. 14 No. 4 (2007): December 2007 Vol. 14 No. 3 (2007): September 2007 Vol. 14 No. 2 (2007): June 2007 Vol. 14 No. 1 (2007): March 2007 Vol. 13 No. 4 (2006): December 2006 Vol. 13 No. 3 (2006): September 2006 Vol. 13 No. 2 (2006): June 2006 Vol. 13 No. 1 (2006): March 2006 Vol. 12 No. 4 (2005): December 2005 Vol. 12 No. 3 (2005): September 2005 Vol. 12 No. 2 (2005): June 2005 Vol. 12 No. 1 (2005): March 2005 More Issue

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name
-

Contact Email
-

Phone
-

Journal Mail Official
hayati_j_biosci@cbn.net.id

Editorial Address
-

Location
Kota bogor,

Jawa barat

INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name -

Contact Email -

Phone -

Journal Mail Official hayati_j_biosci@cbn.net.id

Editorial Address -

Location Kota bogor, Jawa barat INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
-

Contact Email
-

Phone
-

Journal Mail Official
hayati_j_biosci@cbn.net.id

Editorial Address
-

Location
Kota bogor,

Jawa barat

INDONESIA