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HAYATI Journal of Biosciences

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Published by Institut Pertanian Bogor

ISSN : 19783019 EISSN : 20864094 DOI : -

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

HAYATI Journal of Biosciences (HAYATI J Biosci) publishes articles and short communication in tropical bioscience fields such as development, biotechnology, biodiversity and environmental issues. HAYATI J Biosci covers wide range of all life forms topics including virus, microbes, fungi, plants, animal and human. HAYATI J Biosci has been also indexed/registered in Crossref, DOAJ, CABI, EBSCO, Agricola and ProQuest.

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Articles 6 Documents

Search results for , issue "Vol. 26 No. 1 (2019): January 2019" : 6 Documents clear

Behavior, Histopathology and Physiological Responses of Rat Fed Diets Containing Growth Hormone Transgenic Fish Meal Alimuddin Alimuddin; Sri Murtini; Nurly Faridah; Sri Nuryati
HAYATI Journal of Biosciences Vol. 26 No. 1 (2019): January 2019
Publisher : Bogor Agricultural University, Indonesia

The animal model response against genetically modified product may provide food safety information. This study was performed to observe behavior, histopathology and physiological responses of Wistar rat fed on the diet containing growth hormone (GH) transgenic common carp (Cyprinus carpio) meal (Ccm). Thirty rats of three-month-old (BW: 115.67–139.50g) were divided into five treatments (six rats per treatment). The treatments were rats fed on the commercial diet without Ccm (control), a re-pelleted diet containing 15% (NT-15) and 45% non-transgenic Ccm (NT-45), a re-pelleted diet containing 15% (TG-15) and 45% GH transgenic Ccm (TG-45). Rats were kept for three weeks, fed twice a day according to treatment by 30 g/day/rat and water was provided ad-libitum. Rat behavior was observed every day during feeding. Serum glutamic oxaloacetic transaminase (SGOT), serum glutamic pyvuric transaminase (SGPT), urea, and creatinine were measured at initial and the end of the experiment. Histology observation was made for liver, kidney, and brain tissues. Our results showed that behavior, histopathology, SGOT, SGPT, urea and creatinine levels of rat fed with control, NT- and TG-Ccm diets were similar. All rats survived until the end of the experiment. Thus, within the dose level of this study, GH transgenic Ccm showed no toxicity or effect on behavior and physiology of Wistar rats.

Identification of Banana Plants from Unmanned Aerial Vehicles (UAV) Photos Using Object Based Image Analysis (OBIA) Method (A Case Study in Sayang Village, Jatinangor District, West Java) Agung Budi Harto; Prila Ayu Dwi Prastiwi; Farah Nafisa Ariadji; Deni Suwardhi; Fenny M. Dwivany; I Wayan Nuarsa; Ketut Wikantika
HAYATI Journal of Biosciences Vol. 26 No. 1 (2019): January 2019
Publisher : Bogor Agricultural University, Indonesia

Banana is one of the leading fruit commodities of Indonesia and ranks the sixth position as one of the largest banana producers in the world. There are more than 200 types of banana in Indonesia. The utilization of bananas is influenced by the local culture, where in every 10 horticultural households, 5 of them plant bananas both as garden plants or field plants. This horticultural crop is expectantly being one of the actions to improve economic prosperity especially in rural areas. In maintaining the diversity of the growing bananas in rural areas, a geospatial approach to identify the vegetation is required. Remote sensing technology is one of the solutions to observe and to develop banana plants with one of the methods namely Object Based Image Analysis (OBIA). This method consists of segmentation, classification, and validation. In classification process, the OBIA method distinguishes objects not only based on pixel values but also on the basis of the shape, area, and texture around them. This research has proven that the classification using OBIA method is better than the traditional classification such as maximum likelihood classification method to identify banana plants. OBIA method can quickly identifies the vegetation and non-vegetation, also the regular plants and banana plants.

Bioactive Compound from Extract Filtrat Streptomyces sp.Sp1. as Biocontrol of Vibriosis on Larvae of Macrobrachium rosenbergii shrimps Retno Kawuri; IBG Darmayasa
HAYATI Journal of Biosciences Vol. 26 No. 1 (2019): January 2019
Publisher : Bogor Agricultural University, Indonesia

Production of Macrobrachium rosenbergii shrimp was limited by vibriosis disease. Streptomyces can be used as an alternative control of vibriosis which is caused by Vibrio anguillarum. Aplication of Streptomyces sp.1 culture could give significant different impacts (p<0.05) on the percentage of survival (SR) of shrimp larvae that had been infected by V. anguillarum compared to the control. This study was conducted to identify and determine bioactive compound of Streptomyces sp.1. The presence of bioactive compound was confirmed by gas chromatography-mass spectrometry (GC-MS) analysis. Identification of Streptomyces sp.1 by 16S rRNA partial gene sequencing method. Streptomyces sp.1 was identified to be Streptomyces sp.Sp1. There were 35 chemical compounds identified, the chemical compounds which have antimicrobial properties identified in the n butanol extract of Streptomyces sp.Sp1 are 3-Hexanone, 2-methyl (43%), n Butyl ether (19%) Nonane (CAS) n-Nonane (5.6%), Decane (CAS) n-Decane (3.2%), Hexacosane (CAS) n-Hexacosane (1.9%), Tetracontane (1.3%), Heneicosane (1.9%), Hexadeconoic acid, methyl ester (0.3%), Butane,1,1-dibutoxy (0.3%), and Limonene (0.3%). Benzeneacetic acid, 3-methoxy-.alpha.,4-bis[(tri (0.3%). These results suggested that Streptomyces sp.Sp1 had good general antimicroba activity and might have potential biocontrol antagonist Vibrio anguillarum.

Production and Characteristics of Yeast Dextranase from Soil Vestika Iskawati Wahidul Hijah; Titi Candra Sunarti; Anja Meryandini
HAYATI Journal of Biosciences Vol. 26 No. 1 (2019): January 2019
Publisher : Bogor Agricultural University, Indonesia

The existence of dextran in sugar cane juice is a major problem in the sugar industry, causing substantial losses. Treatment of dextran through enzymatic hydrolysis using dextranase is highly recommended as the most suitable method at this time because this is more effective and more economical. This study investigated the production and characterization of dextranase from local isolate yeast to degrade dextran on sugar cane juice. The selected yeast was identified on the basis of molecular identification. Dextranase was produced from the culture with the best carbon and nitrogen sources then was characterized. Application of enzyme was also evaluated. As a selected isolate, F4 had the closest relationship with Pichia kudriavzevii. The highest production of dextranase was induced by the supplementation of glucose and combination of yeast extract and peptone. The enzyme had optimum working condition at pH 7, temperature at 30°C and it is more stable at 4°C of storage temperature. The cation Na+ played key role as co-factor while K+ and Ca2+ were detected as inhibitor of the enzyme. Dextranase from F4 isolate can hydrolyze dextran both in pure and in mixed dextran substrate, but with a lower hydrolysis rate.

Ground Vegetation Diversity on Different Type of Riverbank Along Ciliwung River in Bogor City, West Java Amarizni Mosyaftiani; Kaswanto Kaswanto; Hadi Susilo Arifin
HAYATI Journal of Biosciences Vol. 26 No. 1 (2019): January 2019
Publisher : Bogor Agricultural University, Indonesia

Ciliwung riverbank has been affected by settlement occupation. Both concrete and another retaining wall system have been built to avoid flood and erosion to the settlement that has existed adjacent to the river. Built environment as human disturbance can trigger the change of species richness of ground vegetation in the Ciliwung riverbank of Bogor City. The research objective is to study the difference of ground vegetation biodiversity in three different types of riverbank based on its condition: natural, semi-natural, and constructed riverbanks. Point method and photographic sampling are used. Five replications of a square metre plot at three transects were placed at four sites sampling along 250 m each from the three different types of riverbank. Images of the plots are trained using sample point to overcome the difficulties in the field observation and statistical analysis. The result discovered that species richness in the natural riverbank is significantly higher than in semi-natural and constructed riverbank. Yet, no significance of species richness is shown between semi-natural and constructed riverbank. Total species of ground vegetation that we found were 55 species. Each different type of riverbank has its own highest species coverage: Asystasia nemorum (55.00%) in the natural riverbank, Pilea nummulariifolia (33.07%) in the semi-natural riverbank, and Pogonatherum crinitum (52.80%) in the constructed riverbank. The study concluded that the construction in the riverbank largely altered the biodiversity of ground vegetation in the urban riverbank. The outcomes suggest that the remnant of natural riverbank in the urban landscape should be highly protected from urban development to preserve its biodiversity. Also, species found could be potentially developed as landscape plants to support urban river planning, design, and management.

Genetic Variation of Calpastation Gene of Indigenous Bali Cattle (Bos javanicus) in Indonesia Endang Tri Margawati; Slamet Diah Volkandari; Indriawati Indriawati; Emma M. Svensson
HAYATI Journal of Biosciences Vol. 26 No. 1 (2019): January 2019
Publisher : Bogor Agricultural University, Indonesia

Calpastatin is one of gene markers affecting meat tenderness. The study aimed to evaluate genetic variation of calpastatin (CAST) gene of Bali cattle (Bos javanicus) in lndonesia. A total of 61 samples consisting of 21 Bali cattle, 22 Ongole cattle (Bos indicus), and 18 Friesian Holstein (FH) cattle (Bos taurus) were applied. The Ongole and FH cattle were involved for breed comparison. DNA was extracted from fresh blood using a High Salt method and measured their quality by a Spectrophotometer. A 523 bp of Calpastatin gene fragment was amplified by Polymerase Chain Reaction and Restriction Fragment Polymorphism (PCR-RFLP) technique with RsaI restriction enzyme for genotyping. Result showed that two variants alleles (C and G) and three genotypes (CC, GC, GG) were found in those Bali, Ongole and FH samples. Allele G was dominant allele with the highest G allele was in Bali cattle population (0.88). The higher percentage of allele C was found in Ongole and Friesian Holstein compared to that in Bali cattle. The Ongole breed tends to have a potential source of lean meat quality. This finding identified that genetic variation of CAST gene was exist in Bali cattle and adapted cattle of Ongole and FH in Indonesian.

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