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All Journal HAYATI Journal of Biosciences Agrotrop : Journal on Agriculture Science Buletin Udayana Mengabdi Bumi Lestari BIOMA : Jurnal Biologi Makassar Asian Journal of Agriculture Journal of Health Sciences and Medicine Indonesian Biodiversity Journal Indonesian Biodiversity Journal Metamorfosa: Journal of Biological Science Open Access DRIVERset
Retno Kawuri
Program Studi Biologi Fakultas Matematika Ilmu Pengetahuan Alam, Universitas Udayana
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health Veterinary

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PENGARUH SINAR ULTRAVIOLET TERHADAP PERTUMBUHAN BAKTERI Enterotoxigenic E.coli (ETEC) PENYEBAB PENYAKIT DIARE Risky Vidika Apriyanthi; I Gst. AA Ratnawati; Retno Kawuri
BIOMA : JURNAL BIOLOGI MAKASSAR Vol. 6 No. 1 (2021): Bioma, Januari - Juni 2021
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/bioma.v6i1.12157

Abstract

. Telah dilakukan penelitian mengenai pengaruh sinar ultraviolet terhadap pertumbuhan bakteri Enterotoxigenic E.coli  (ETEC). Penelitian dilakukan untuk mengetahui jarak, lama penyinaran, persentase kematian dan pengaruh sinar ultraviolet yang dapat mematikan bakteri ETEC. Metode penanaman dan penghitungan total koloni bakteri yang digunakan adalah plating method. Selanjutnya dilakukan pewarnaan gram untuk melihat bentuk sel bakteri uji.Hasil penelitian menunjukkan, persentase kematian bakteri ETEC tertinggi yaitu pada jarak 15 cm dengan lama penyinaran 15 menit.dengan intensitas 40 lux, sebesar 91,36%. Sedangkan pada jarak 30 cm dengan lama penyinaran 5 menit dan intensitas 19 lux, hanya berhasil membunuh bakteri sebesar 19,52%. Sel bakteri ETEC yang telah diberi penyinaran, bentuk sel bakteri ETEC mengalami perubahan menjadi elips, ada yang mengalami lysis dan susunan rantai terputus. Kata Kunci : Ultraviolet, jarak, lama penyinaran, Enterotoxigenic E.coli  (ETEC).  
Bioactive Compound from Extract Filtrat Streptomyces sp.Sp1. as Biocontrol of Vibriosis on Larvae of Macrobrachium rosenbergii shrimps Retno Kawuri; IBG Darmayasa
HAYATI Journal of Biosciences Vol. 26 No. 1 (2019): January 2019
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (913.24 KB) | DOI: 10.4308/hjb.26.1.15

Abstract

  Production of Macrobrachium rosenbergii shrimp was limited by vibriosis disease. Streptomyces can be used as an alternative control of vibriosis which is caused by Vibrio anguillarum. Aplication of Streptomyces sp.1 culture could give significant different impacts (p<0.05) on the percentage of survival (SR) of shrimp larvae that had been infected by V. anguillarum compared to the control. This study was conducted to identify and determine bioactive compound of Streptomyces sp.1. The presence of bioactive compound was confirmed by gas chromatography-mass spectrometry (GC-MS) analysis. Identification of Streptomyces sp.1 by 16S rRNA partial gene sequencing method. Streptomyces sp.1 was identified to be Streptomyces sp.Sp1. There were 35 chemical compounds identified, the chemical compounds which have antimicrobial properties identified in the n butanol extract of Streptomyces sp.Sp1 are 3-Hexanone, 2-methyl (43%), n Butyl ether (19%) Nonane (CAS) n-Nonane (5.6%), Decane (CAS) n-Decane (3.2%), Hexacosane (CAS) n-Hexacosane (1.9%), Tetracontane (1.3%), Heneicosane (1.9%), Hexadeconoic acid, methyl ester (0.3%), Butane,1,1-dibutoxy (0.3%), and Limonene (0.3%). Benzeneacetic acid, 3-methoxy-.alpha.,4-bis[(tri (0.3%). These results suggested that Streptomyces sp.Sp1 had good general antimicroba activity and might have potential biocontrol antagonist Vibrio anguillarum.
PELATIHAN HIDROPONIK DI SMAN 1 DENPASAR, BALI M. Pharmawati; N. N. Wirasiti; I.G.A.S. Wahyuni; R. Kawuri
Buletin Udayana Mengabdi Vol 16 No 2 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (252.003 KB)

Abstract

Hidroponik merupakan salah satu teknik budidaya tanaman khususnya sayuran yang memiliki beberapa keuntungan antara lain tidak memerlukan lahan yang luas, nutrisi seimbang, bebas gulma serta produksi yang tinggi. Teknik budidaya sayuran secara hidroponik dapat memanfaatkan pekarangan rumah atau halaman sekolah. Oleh karena itu, diperlukan kegiatan pengenalan bertanam sayuran secara hidroponik. Tujuan kegiatan in adalah memperkenalkan dan melatih bertanam sayuran secara hidroponik bagi siswa SMAN 1 Denpasar. Metode yang digunakan terdiri dari metode ceramah, praktek dan diskusi yang meliputi pengenalan jenis-jenis tanaman sayuran, pengenalan berbagai teknik hidroponik serta teknik bertanam mulai dari persemaian, pembibitan, penanaman dan pemeliharaan. Sistem hidroponik yang dipilih adalah sistem hidroponik statis sederhana dengan menggunakan wick (sumbu). Kegiatan pertama di SMAN 1 Denpasar diikuti oleh 18 siswa dan dua guru pendamping. Evaluasi dilakukan melalui antusias dan keaktifan murid serta pertumbuhan tanaman yang dipraktekkan. Sumbu yang terbuat dari kain flannel mengakibatkan pertumbuhan tanaman yang lebih baik dibandingkan sumbu kompor. Kegitan kedua merupakan pengenalan teknik hidroponik aktif diikuti oleh 8 orang siswa dan 2 guru pendamping.
JENIS, STATUS DAN PEMANFAATAN TUMBUHAN JENIS POHON DI DESA ADAT BATURNING, KECAMATAN ABIANSEMAL, KABUPATEN BADUNG, BALI N. P. Adriani Astiti; Retno Kawuri; I K. Ginantra
Bumi Lestari Journal of Environment Vol 8 No 2 (2008)
Publisher : Environmental Research Center (PPLH) of Udayana University

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Abstract

There were 65 species of trees have been identified in Baturning village, District of Abiansemal, Regency of Badung, Bali, which were spread out around the river bank, cemetery (around Dalem temple), around the settlement, non-irrigated farm (plantation or “tegalan”) and rice field. Thirty eight (38) % out of them were observed to be available around the settlement. Most of trees in Baturning (61 species or 94%) have been used by the local community. The rest were uncultivated. From the use, trees in Baturning most were used for human consumption (24%). On the village has not been found protected species, even though in the village were found 22 species of trees which considered to be rare in Bali, namely: Bayur, Bentenung, Beringin, Cempaka, Ee, Jaka, Jeruk Bali, Juwet, Kelor, Kendal, Klampuak, Lempeni, Lenggung, Mahoni, Mengkudu, Palem Raja, Poh-Pohan, Pule, Sandat, Sentul, Udu, and Wani.
Streptomyces sp. Sebagai Biofungisida Patogen Fusarium oxysporum (Schlecht.) f.sp. lycopersici (Sacc.) Snyd. et Hans. Penyebab Penyakit Layu Pada Tanaman Tomat (Solanum lycopersicum L.) NURI MANDAN SARI; RETNO KAWURI; KHAMDAN KHALIMI
Agrotrop : Journal on Agriculture Science Vol 2 No 2 (2012)
Publisher : Fakultas Pertanian Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (576.794 KB)

Abstract

A research was conducted to isolate Streptomyces sp. of soil Udayana University campus in theBukit-Jimbaran, to obtain the most effective Streptomyces sp. which is effective in inhibit the growth ofFusarium oxysporum f.sp. lycopersici, and to test response of tomato plants with Streptomyces sp.culture against Fusarium wilt desease. Implementation phases of the research consisted of isolation andidentification of Streptomyces sp, test the inhibition against F. oxysporum f.sp. lycopersici, and in vivotest used by dyeing the roots of the tomato plant (Solanum lycopersicum) with Fusarium spores andafter 30 seconds the roots were dyeing Streptomyces culture. Furthermore, sterile soil in polybagwatered by Fusarium spores and Streptomyces culture at the same time. Based on morphologicalcharacteristic it found five isolates of Streptomyces sp.. The antagonist test showed Streptomyces sp.1 had ability (75%) against Fusarium, Streptomyces sp 2 (68,3%), Streptomyces sp. 3 (71,6%),Streptomyces sp. 4 (63,3%), and Streptomyces sp. 5 (21,6%). All Streptomyces suppressed thegrowth of Fusarium on tomato plants in glass house (p<0,05). Streptomyces sp.3 suppressed Fusariumwilt disease in tomato from 88% in control to 20%.
Penggunaan Streptomyces sp. Sebagai Biokontrol Penyakit Layu Pada Tanaman Cabai Merah (Capsicum annuum L.) yang Disebabkan Oleh Fusarium oxysporum f.sp. capsici ANINDA OKTAVIA RAHARINI; RETNO KAWURI; KHAMDAN KHALIMI
Agrotrop : Journal on Agriculture Science Vol 2 No 2 (2012)
Publisher : Fakultas Pertanian Universitas Udayana

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Abstract

A research has been conducted to find out Streptomyces bacteria at Bukit Jimbaran, to inhibitionpotency of Streptomyces sp. to pathogenic fungi Fusarium oxysporum f.sp. capsici, and to find outantifungal activity of Streptomyces filtrate to F.oxysporum f.sp. capsici in chili (Capsicum annuumL.) plants. Streptomyces sp. isolation was done by platting method with selective media YMA (ISP4).Identification of Streptomyces sp. used Bergey’s book entitled Manual Determinative Bacteriology.Test inhibition against F.oxysporum f.sp. capsici and in vivo test used by dying the roots of the chili(C.annuum L.) plant with F.oxysporum f.sp. capsici and after 30 seconds the roots were dying withStreptomyces sp. culture, furthermore sterile soil on polybag watered by F.oxysporum f.sp. capsicispore and Streptomyces sp. culture at the same time. The result found five isolates Streptomyces sp.with different morphological. The antagonis test showed Streptomyces sp. 4 had ability (82%) againstFusarium, Streptomyces sp.1 (72%), Streptomyces sp.2 (64%), Streptomyces sp.3 (76%), andStreptomyces sp. 5 (32%). All Streptomyces suppressed the growth of Fusarium on chili plants inglass house (p<0,05). Streptomyces sp.4 suppressed Fusarium wilt disease in chili from 80% in controlto 8%.
THE ESSENTIAL OIL CONTENTS OF JERINGAU (Acorus calamus L.) RHIZOMES AND THEIR ANTIFUNGAL ACTIVITY AGAINST Candida albicans Wiwik Susanah Rita; Retno Kawuri; I Made Dira Swantara
Journal of Health Sciences and Medicine Vol 1 No 1 (2017): JHSM (Febuary 2017)
Publisher : Institute for Research and Community Services Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (463.011 KB) | DOI: 10.24843/JHSM.2017.v01.i01.p09

Abstract

Acorus calamus L. rhizome was trusted having antibacterial activity. This study aimed to identify the compounds in the Acorus Calamus L. rhizomes essential oils and to recognize the antifungal activity of the oils against Candida albicans. The extraction of essential oils from rhizome was carried out by steam distillation technique. Identification of compounds in the oils was conducted by Gas Chromatography- Mass Spectroscopy (GCMS), while the antifungal test against Candida albicans was done by well diffusion method. Extraction of 10 kg of rhizomes produced 16.53 mL essential oil with a yield of 0.1653% (? = 1.066), the oil was brownish yellow and very flavorful. GC-MS analysis showed that the essential oil contained 11 compounds, they are (E)-3,7 dimethyl-1,3,6-Octatriene (trans-?-Ocimene) (3,73%), linalool (1,07%), ?-elemene (1,15%), trans methyl isoeugenol (7,68%), shyobunon (15,74%), bicyclogermakren (0,93%), dehidroxy-isocalamendiol (2,61%), ?-calacorene (3,34%), euasarone (26,84), cis-asarone (18,62%); dan trans- asarone (18,29%). Antifungal activity test showed that the growth and biomass inhibition of C. albicans increased with the increase of the oil concentration. Minimum Inhibitory Concentration (MIC) of essential oil toward C. albicans was 1% with the inhibition of 7.83 mm.
MORPHOMETRY OF EAGLE SPECIES (FAMILY ACCIPITRIDAE) AT BALI BIRD PARK Iriani Setyawati; L.P. Eswaryanti Kusuma Yuni; Retno Kawuri; Ni Made Suartini; Ni Wayan Sudatri; I Gede Agus Pradana Putra
Indonesian Biodiversity Journal Vol. 3 No. 3 (2022): December 2022
Publisher : Universitas Negeri Manado

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Abstract

This study measured the morphometry of raptor species (Family Accipitridae) in Bali Bird Park (BBP) namely Nisaetus cirrhatus (Changeable Hawk Eagle), Elanus caeruleus (Black Winged Kite), Spilornis cheela (Crested Serpent Eagle) and Haliaeetus leucogaster (White Bellied Sea Eagle). Measurements were made with meter tape and caliper on birds that were in their resting period. Raptor at BBP generally rest for 6 months until experience molting before being retrained for 4-5 months, then the birds will be ready to perform bird show for the next 9-12 months. Our measurements obtained the largest bird was Haliaeetus leucogaster with a total body length of 75 cm, wings length 83.5-85 cm, tail length 26 cm, head size (8 cm length and 7 cm width), upper beak (8 cm length and 2.5 cm thick) and lower beak (3 cm length and 1 cm thick), lower limb length (femur 17 cm, tibia 9 cm, metatarsus 11 cm, middle toe 6.5 cm and grip 9 cm). The smallest bird was Elanus caeruleus with a total body length of 33 cm, wing length 39-45 cm, tail length 19 cm, head size (8 cm length, 5.5 cm width), upper beak (2.5 cm length and 0.5 cm thick) and lower beak (1 cm length and 0.3 cm thick), lower limb length (femur 8 cm, tibia 4 cm, metatarsus 5 cm, middle toe 3 cm and grip 5 cm) The morphometry of the species Nisaetus cirrhatus and Spilornis cheela were between the two other bird species.
PELATIHAN AKLIMATISASI BIBIT STROBERI HASIL KULTUR MERISTEM BAGI PETANI STROBERI PANCASARI R. Dwiyani; K.B. Susrusa; I.A.P. Darmawati; G.N.A.S. Wirya; I.G.A. Gunadi; N.N.A. Mayadewi; H. Yuswanti; K.A. Yuliadhi; T.A. Phabiola; R. Kawuri; Y. Fitriani
Buletin Udayana Mengabdi Vol 22 No 3 (2023): Buletin Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/BUM.2023.v22.i03.p10

Abstract

Abstracts The community service activity entitled "Training for Acclimatization of Strawberry Seeds from Meristem Culture for Pancasari Strawberry Farmers" has been completed. This activity was motivated by the problem of seeds by strawberry farmers in Pancasari, where farmers needed healthy seeds from tissue culture, because through propagation with runners, crop yields decreased drastically due to virus attacks. The solution is the use of seeds from meristem culture. Acclimatization training by farmers is important because removing seeds from culture bottles and then growing them on media ex vitro requires skills and understanding of acclimatization theory. The purpose of this service was to provide strawberry acclimatization skills to farmers, so that farmers can acclimatize themselves to meristem cultured strawberries produced by the Tissue Culture Laboratory of the Faculty of Agriculture Udayana University. This activity was based on the results of strawberry meristem culture research in 2019 funded by the ministry of research technology and higher education Republic of Indonesia through a national competitive grant. The results of the current community service concluded several things as follows: The training participants, especially farmers, have succeeded in carrying out their own acclimatization practice; Strawberry seedlings grown in the highlands (Pancasari) had better vigor than those grown in the lowlands (Denpasar). Keywords: acclimatization, meristem culture, Pancasari, strawberry, virus.
MORPHOMETRY OF EAGLE SPECIES (FAMILY ACCIPITRIDAE) AT BALI BIRD PARK Iriani Setyawati; L.P. Eswaryanti Kusuma Yuni; Retno Kawuri; Ni Made Suartini; Ni Wayan Sudatri; I Gede Agus Pradana Putra
Indonesian Biodiversity Journal Vol. 3 No. 3 (2022): December 2022
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v3i3.5733

Abstract

This study measured the morphometry of raptor species (Family Accipitridae) in Bali Bird Park (BBP) namely Nisaetus cirrhatus (Changeable Hawk Eagle), Elanus caeruleus (Black Winged Kite), Spilornis cheela (Crested Serpent Eagle) and Haliaeetus leucogaster (White Bellied Sea Eagle). Measurements were made with meter tape and caliper on birds that were in their resting period. Raptor at BBP generally rest for 6 months until experience molting before being retrained for 4-5 months, then the birds will be ready to perform bird show for the next 9-12 months. Our measurements obtained the largest bird was Haliaeetus leucogaster with a total body length of 75 cm, wings length 83.5-85 cm, tail length 26 cm, head size (8 cm length and 7 cm width), upper beak (8 cm length and 2.5 cm thick) and lower beak (3 cm length and 1 cm thick), lower limb length (femur 17 cm, tibia 9 cm, metatarsus 11 cm, middle toe 6.5 cm and grip 9 cm). The smallest bird was Elanus caeruleus with a total body length of 33 cm, wing length 39-45 cm, tail length 19 cm, head size (8 cm length, 5.5 cm width), upper beak (2.5 cm length and 0.5 cm thick) and lower beak (1 cm length and 0.3 cm thick), lower limb length (femur 8 cm, tibia 4 cm, metatarsus 5 cm, middle toe 3 cm and grip 5 cm) The morphometry of the species Nisaetus cirrhatus and Spilornis cheela were between the two other bird species.
Co-Authors Anak Agung Gde Raka Dalem Anak Agung Ketut Darmadi Andreas Putro Ragil Santoso, Andreas Putro Ragil ANINDA OKTAVIA RAHARINI Aryadi Millenia Saputra Bayu Putri Handayani Chandrika, Carmen Gisela Darmadi, A.A. Ketut Diah Kharismawati Djereng Dwi Ariani Yulihastuti Felisitas Meli Podhi Gusti Ngurah Alit Susanta Wirya Gusti Ngurah Wisnanda Putra H. Yuswanti Hanifah, Wafa’ Nur I Gede Agus Pradana Putra I Gusti Agung Ayu Ratnawati I Ketut Ginantra I KETUT MUKSIN I Komang Adi Widyastama I Made Dira Swantara I Made Sara Wijana I Putu Gede Ardhana I WAYAN EKA DHARMAWAN I Wayan Yogi Widyastana I.G.A. Gunadi I.G.A.S. Wahyuni Ida Ayu Putri Darmawati Ida Bagus Gede Darmayasa Ilham . Inna Narayani IRIANI SETYAWATI Ivani Dayanara Job Nico Subagio K.A. Yuliadhi K.B. Susrusa Kadek Aryndha Sukma Prabaswari KAMUHI, RINI HERMINA Khamdan Khalimi Khotima Dwi Cahya Luh Made Ary Somia Vagestini LUH Puru ESWARYANTI KUSUMA YUNI Made Mega Yuliasari Made Mira Pratiwi MADE PHARMAWATI Made Ria Defiani MADE SUSUN PARWANAYONI Meitini Proborini Wahyuni Muhammad Inas Riandi N.N.A. Mayadewi NI KADEK LOSIANI Ni Ketut Febri Antini Ni Ketut Yuliana Sari Ni LUH ARPIWI Ni Luh Cipta Ayumi Nellawati Ni Luh Suriani Ni Luh Watiniasih Ni Made Suartini Ni Made Widyasari Ni Nyoman Wirasiti Ni Putu Adriani Astiti NI Putu Diah Septiani Putri Ni Putu Niti Rahayu Ni Wayan Desi Bintari Ni Wayan Purni wirathi Ni Wayan Sudatri NURI MANDAN SARI Putri Permata Putu Anggita Sekar Pratiwi Putu Anjarina Putu Ayu Parwati Putu Ayu Sukmawati Putu Cindy Arista Revi Juniar Nutrihidayah Rindang Dwiyani Risha Masfufah Risky Vidika Apriyanthi Rochmalia Juniarti Putri Rochmalia Juniarti Putri SANG KETUT SUDIRGA SANTIKA, I PUTU BENNY SARMILA TASNIM Suta Arta TRISNA AGUNG PHABIOLA WIWIK SUSANAH RITA Y. Fitriani Yan Ramona Yuyun Fitriani