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Mujiyo Mujiyo
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Pusat Penelitian dan Pengembangan Pangan, Gizi dan Kesehatan Masyarakat (P4GKM) Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) Universitas Sebelas Maret (UNS) Jl. Ir. Sutami No.36A, Jebres, Kec. Jebres, Kota Surakarta, Jawa Tengah 57126
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AgriHealth: Journal of Agri-food, Nutrition and Public Health
Published by Universitas Sebelas Maret
ISSN : 27220656     EISSN : 27220648     DOI : https://dx.doi.org/10.20961/agrihealth
Core Subject : Health, Education, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Education Environmental Science Health Professions Public Health
AgriHealth: Journal of Agri-food, Nutrition and Public Health merupakan wadah publikasi ilmiah hasil kajian penerapan ilmu pengetahuan dan teknologi kepada para pemangku kepentingan (stakeholder) di bidang pertanian ramah lingkungan, pangan, gizi dan kesehatan masyarakat yang meliputi: Budidaya Pertanian Teknologi Pangan Peternakan Penyuluhan dan Komunikasi Pertanian Sosial Ekonomi Pertanian Gizi Kesehatan Masyarakat Kebidanan
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Agronomi dan Ilmu Tanaman
Articles 102 Documents
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Prevalence and Molecular Detection of Toxigenic Genes of Bacillus cereus in Rice-Based Street Foods in Tasikmalaya, Indonesia Maerani Maerani; Mufti Ghaffar; Fadhiya Munggarani; Dina Aulia Nuraeni; Pandu Legawa Ismaya; Hadi Yusuf Faturochman
AgriHealth: Journal of Agri-food, Nutrition and Public Health Vol 7, No 1 (2026): AgriHealth in Press April 2026
Publisher : Research and Development Center for Food, Nutrition and Public Health (P4GKM) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/agrihealth.v7i1.106053

Abstract

Bacillus cereus is a spore-forming foodborne pathogen frequently associated with rice-based foods and capable of causing emetic and diarrheal illnesses through the production of heat-stable and heat-labile toxins. This study investigated the prevalence, contamination levels, and toxigenic gene profiles of B. cereus in rice-based street foods. A total of 12 samples, including fried rice, chicken porridge, and nasi kuning, were collected from street vendors in Kahuripan Sub-district, Tasikmalaya, Indonesia. Presumptive B. cereus was isolated using mannitol yolk polymyxin agar (MYPA) as a selective medium and confirmed biochemically and molecularly by PCR targeting the gyrB gene. Toxigenic potential isolate was evaluated by detecting the nheA, hblD, cytK, entFM, and ces genes. Presumptive B. cereus was detected in 91.67% (11/12) of samples, while molecular confirmation identified gyrB-positive isolates in 42% (5/12) of samples, originating from fried rice and chicken porridge. No significant association was found between food type and gyrB detection (p > 0.05). Contamination levels ranged from < 1.5 × 10¹ to 2.95 × 10⁶ CFU g-1. All confirmed isolates harbored at least one toxigenic gene, with nheA being the most prevalent (80%), followed by cytK (60%), entFM (40%), ces (40%), and hblD (20%). Notably, one of the fried rice isolates carried all 5 toxin-related genes, indicating a high virulence potential. These findings demonstrate that rice-based street foods in Tasikmalaya may pose a significant public health risk due to contamination with toxigenic B. cereus. Enhanced hygiene practices, strict temperature control, and molecular surveillance are essential to reduce the risk of foodborne illness associated with street-vended rice products.
The Effect of Land Clearing Techniques on the Chemical and Biological Character of Soil in Sugarcane Fields Anna Kusumawati; Lintang Panjali Siwi Pambayun
AgriHealth: Journal of Agri-food, Nutrition and Public Health Vol 6, No 1 (2025): April
Publisher : Research and Development Center for Food, Nutrition and Public Health (P4GKM) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/agrihealth.v6i1.102705

Abstract

Land clearing after harvest in sugarcane cultivation is often done by burning. However, burning influences the ecosystem, such as the chemical and biological characteristics of soil. This research aims to analyze the impact of different methods of clearing sugarcane fields on the chemical and biological conditions of the soil. This research used a non-factorial, completely randomized design (CRD) with two treatments: land clearing with burning and without burning. Each treatment was carried out on an area of 50 m2 with two blocks as repetitions. For chemical and biological analysis, soil samples were taken from pitfall traps and soil monoliths (1 m x 1 m x 30 cm). Two treatments showed significant differences in the total N and available P, and burning reduced 21% the number of organisms significantly. There were significant differences between the two treatments regarding the macrofauna diversity index, species evenness value, and dominance index. Post-harvest burning significantly reduces total N and available P and negatively impacts biological indicators such as organism numbers, diversity, evenness, and dominance index. This decrease in organic matter causes a reduction in the populations of organisms, thus disrupting soil health. Therefore, this study recommends that sugarcane residues be returned to the land as a source of organic matter to maintain soil health, biodiversity, and optimal productivity.

Page 11 of 11 | Total Record : 102

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