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Jurnal Sains dan Teknologi Laboratorium Medik
Published by Akademi Kesehatan John Paul II Pekanbaru
ISSN : 25275267     EISSN : 26217708     DOI : https://doi.org/10.52071/jstlm
Core Subject : Health, Science, Education,
Education Health Professions Immunology & microbiology Public Health Other
Jurnal Sains dan Teknologi Laboratorium Medik (JSTLM, e-ISSN: 2621-7708, p-ISSN: 2527-5267) adalah sebuah jurnal yang didedikasikan untuk plublikasi hasil penelitian yang berkualitas dalam bidang sains dan laboratoium medik. semua publikasi di jurnal Jstlm bersifat akses terbuka yang memungkinkan artikel tersedia secara bebas online tanpa berlangganan apapun. Jurnal Sains dan Teknologi Laboratorium Medik (JSTLM) dikelola secara profesional dalam hal membantu para akademisi, peneliti dan praktisi untuk menyebarkan hasil penelitiannya. focus dan scope Jurnal Sains dan Teknologi Laboratorium Medik (JSTLM) adalah sains, mikrobiologi, parasitologi, hematologi, kimia klinik, dan toksikologi.
Arjuna Subject : Umum - Umum
Articles 9 Documents
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Search results for , issue "Vol 10 No 2 (2024): November 2024" : 9 Documents clear
Uji Efektivitas Daun Bawang Dayak (E. Palmifolia) Menggunakan Pelarut Etil Asetat Terhadap Pertumbuhan Staphylococcus Aureus Dengan Metode Sumuran Jumaqairiah, Siti
Jurnal Sains dan Teknologi Laboratorium Medik Vol 10 No 2 (2024): November 2024
Publisher : Akademi Kesehatan John Paul II Pekanbaru

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52071/jstlm.v10i2.162

Abstract

Dayak leek (Eleutherine palmifolia) is one of the plants that is often used by people for traditional medicine in the Kalimantan region. Based on research, Dayak leeks contain alkaloids, flavonoids, saponins and tannins which can function as antibiotics.The aim of this research is to determine the effectiveness of Dayak leeks (Eleutherine palmifolia) using ethyl acetate solvent on the growth of Staphylococcus aureus using the well method. The research design used was true experimental, where this study used a treatment group and a positive control group (clindamycin 2 mcg).The results of the research showed that in the test Dayak leek extract with concentrations of 60%, 65%, 70%, 75% and 80% formed an average diameter of the inhibition zone of 2 mm, 3.8 mm, 4 mm, 4.4 mm and 7 mm in a row. And the control material clindamycin 2 mcg obtained an inhibition zone of 14 mm. So it can be concluded that Dayak leeks can be used as an alternative to antibiotics, especially at high concentrations.Keywords: Dayak leek extract, Staphylococcus aureus, inhibitory power
GAMBARAN KADAR CARBOHYDRATE ANTIGEN 19-9 ( CA 19-9 ) PADA PASIEN YANG DI CURIGAI KANKER PANKREAS DI RSUD ABDUL WAHAB SJAHRANIE SAMARINDA PADA TAHUN 2020-2022 Pondi, Restiyani
Jurnal Sains dan Teknologi Laboratorium Medik Vol 10 No 2 (2024): November 2024
Publisher : Akademi Kesehatan John Paul II Pekanbaru

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52071/jstlm.v10i2.165

Abstract

Pancreatic cancer is one of the most fatal cancers and the leading cause of cancer-related deaths. The survival rate for pancreatic cancer in the United States is reported to be 8%, which is another common type of cancer and in Indonesia itself pancreatic cancer is ranked 17th. Ca 19-9 is a cell produced by pancreatic exocrine gland cancer cells and can be detected in blood tests. The purpose of this study was to determine the Description of Ca 19-9 Levels in Pancreatic Cancer Patients at Abdul Wahab Sjahranie Hospital, Samarinda in 2020-2022. The research design used was the type of Analytical Observational method using Cross Sectional, meaning that the variables studied were only imposed once on a number of subjects who were the research samples. The results of the study were collected from medical records in the clinical pathology laboratory at Abdul Wahab Sjahranie Hospital, Samarinda in 2020-2022. From this study, it was found that there were 72 patients suspected of pancreatic cancer, where the majority were <55 years old, and 57% were male. Furthermore, based on the examination of Ca19-9 levels, it was found that 28 patients experienced levels above normal as much as 39%. The conclusion is that patients suspected of pancreatic cancer who underwent Ca 19-9 examination for the first time obtained results above normal levels, there were 28 patients with a percentage of 39% with an average age of > 65 years.
Perbedaan Sampel Hemolisis dan Non Hemolisis terhadap Nilai Prothombin Time (PT) pada Mahasiswa Poltekkes Kaltim Nashirah, Najwa
Jurnal Sains dan Teknologi Laboratorium Medik Vol 10 No 2 (2024): November 2024
Publisher : Akademi Kesehatan John Paul II Pekanbaru

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52071/jstlm.v10i2.167

Abstract

Haemolysis is the rupture of erythrocyte cells which results in the erythrocytes releasing haemoglobin into the serum or plasma. Haemolysed samples will affect pre-analytical errors in many laboratories. According to the Clinical Laboratory and Standards Institute (CLSI), in the Prothombin Time (PT) and Activated Partial Thromboplastin Time (APTT) testing guidelines, haemolysed samples should not be used for examination due to the potential activation of clotting factors and interference at the end of the measurement point. The purpose of this study was to determine the difference in the results of Prothombin Time (PT) examination in haemolysis samples with non-hemolysis. This type of research is a quasi-experimental design. The research design used is Nonequivalent Control Group Design, which is a design that takes measurements in treated and untreated groups. The samples used in this study were blood sodium citrate haemolysis and non-hemolysis as many as 39 specimens. Data normality analysis using Shapiro Wilk. Paired T-Test test to determine the difference between haemolysis and non-haemolysis samples. The results of this study showed that the average Prothombin Time value of non-hemolysis samples was 15 seconds, mild hemolysis samples were 9 seconds and moderate hemolysis was 8 seconds. In the univariate test results, the results were shortened in mild haemolysis and moderate haemolysis samples. In the bivariate test results obtained normal distributed results and obtained there is a difference in Prothombin Time values in hemolysis and non-hemolysis samples. The conclusion of this study is that there is a significant difference in hemolysis and non-hemolysis samples on the value of Prothombin Time with p value <0.05. Keywords: Haemolysis, Photo Optic, Prothombin Time
Identifikasi Angka Kuman Udara Pada Ruang Pelayanan Di Puskesmas Wonorejo Samarinda nathania, Nhazwa
Jurnal Sains dan Teknologi Laboratorium Medik Vol 10 No 2 (2024): November 2024
Publisher : Akademi Kesehatan John Paul II Pekanbaru

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52071/jstlm.v10i2.169

Abstract

Puskesmas merupakan pusat pelatihan tenaga kesehatan sebagai sarana pelayanan kesehatan, sehingga berpotensi menjadi tempat penularan penyakit dan memungkinkan pencemaran lingkungan. Kualitas udara yang tidak memenuhi persyaratan sebanding dengan angka kuman dalam ruang akan menyebabkan infeksi nosokomial. Tujuan penelitian untuk mengidentifikasi total angka kuman udara pada ruang pelayanan Puskesmas Wonorejo Samarinda. Jenis penelitian adalah deskriptif dengan metode settle plate. Sampel yang digunakan adalah ruang pemeriksaan lansia, ruang pemeriksaan balita dan ruang tindakan pada tanggal 19 Maret – 23 Maret 2024. Hasil penelitian hari pertama, angka kuman pada ruang balita 713 CFU/m3, ruang lansia 918 CFU/m3, ruang tindakan 346 CFU/m3. Hari kedua, ruang balita 692 CFU/m3, ruang lansia 805 CFU/m3, ruang tindakan 437 CFU/m3. Hari ketiga, ruang balita 649 CFU/m3, ruang lansia 791 CFU/m3, ruang tindakan 430 CFU/m3. Hari keempat, ruang balita 289 CFU/m3, ruang lansia 480 CFU/m3, ruang tindakan 282 CFU/m3. Hari kelima, ruang balita 466 CFU/m3, ruang lansia 346 CFU/m3, ruang tindakan 261 CFU/m3. Kesimpulan penelitian adalah angka kuman udara pada ruang balita dan ruang lansia dari hari pertama hingga ketiga belum memenuhi syarat. Namun, hari keempat dan kelima memenuhi syarat. Sedangkan, angka kuman udara pada ruang tindakan dari hari pertama hingga kelima memenuhi syarat sesuai PERMENKES No.7 Tahun 2019,yaitu 200-500 CFU/m3.
ANALISIS KADAR TIMBAL (Pb) PADA PEKERJA PENGELASAN DI DAERAH SAMARINDA SEBERANG DENGAN METODE SPEKTROFOTOMETER SERAPAN ATOM (SSA) Wijaya, Fransiska Angeli Wijaya Angeli
Jurnal Sains dan Teknologi Laboratorium Medik Vol 10 No 2 (2024): November 2024
Publisher : Akademi Kesehatan John Paul II Pekanbaru

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52071/jstlm.v10i2.174

Abstract

Welding is a process of permanently joining metals by heating the metal to the melting point. This process will cause the accumulation of heavy metals in the air. UV and IR radiation is a kind of environmental exposure that can occur during welding. Long-term exposure to welding rays can cause problems with the lens of the eye including loss of transparency, discomfort in activities, itching in the eye area and breathing. Lead that is inhaled and enters the lungs will be associated with blood. Lead that is absorbed by the body will bind to red blood cells and then be distributed into the blood, extracellular fluid, and some soft places (liver, kidneys, and nerves) and mineral tissues (bones and teeth). The purpose of this study was to determine the blood lead levels of welding workers based on length of work, age, and working period with a normal limit value of
Glucose, Ketone and Protein Profile in Diabetes Mellitus Patients Using Urine Strip Test at the Regional Health Laboratory UPTD of Samarinda City Hidayati, Nurul
Jurnal Sains dan Teknologi Laboratorium Medik Vol 10 No 2 (2024): November 2024
Publisher : Akademi Kesehatan John Paul II Pekanbaru

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52071/jstlm.v10i2.175

Abstract

Diabetes mellitus is a disease of the metabolic system with the characteristics of hyperglycemia (an increase in blood sugar levels that exceed normal limits) which is caused by abnormalities in the body's insulin secretion, abnormalities in insulin action or even both. Currently, it is estimated that 25 million or around 9.3% of Indonesia's population in 2020 will experience impaired kidney function due to hypertension and diabetes. The aim of this study was to determine the profile of glucose, ketones and protein in diabetes mellitus sufferers using a urine strip test at the UPTD Regional Health Laboratory of Samarinda City. The type of research used is descriptive. The research population was prolanis patients who had been diagnosed with diabetes mellitus and had their urine checked at the UPTD Regional Health Laboratory of Samarinda City. The samples were Prolanis participants suffering from diabetes mellitus who were examined for urine glucose, ketones and protein using the urine strip test method. The results of the research were that in the urine glucose examination, 4 samples (13%) had 1 positive result and 5 samples (17%) had 2 positive results. In the urine ketone examination, 1 sample (3%) had a positive result of 1. In the urine protein examination, 3 samples (10%) had 1 positive result and 2 samples (7%) had 4 positive results.
The Efektifitas Konsentrasi Pembuatan Handsanitizer Dari Ekstrak Daun Mengkudu (Morinda Citrifolia L.) Bentuk Gel Dalam Menghambat Pertumbuhan Staphylococcus Aureus R. Bara, Libernia Permata
Jurnal Sains dan Teknologi Laboratorium Medik Vol 10 No 2 (2024): November 2024
Publisher : Akademi Kesehatan John Paul II Pekanbaru

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Abstract

Health is very important for life, one way to maintain a healthy body is by washing your hands using a hand sanitizer to kill bacteria. The aim of the research was to determine the effectiveness of hand sanitizer concentration from noni leaf extract (Morinda Citrifolia L.) in inhibiting the growth of Staphylococcus aureus. Staphylococcus aureus bacteria can cause skin and soft tissue infections such as pneumonia, osteomyelitis, and endocarditis. Natural hand sanitizer made from the Noni Plant (Morinda Citrifolia L.) contains alkaloids, flavonoids, saponins, tannins, which are antibacterial. The composition of the hand sanitizer is carbomer, TEA (triethanolamine), alcohol and glycerin. Research Method (Total Plate Count), count colonies on Mueller Hinton Agar media. The results of the research (Handsanitizer concentration 15%) grew colonies with an average number of (74 x 108 CFU/ml), (Handsanitizer concentration 20%) with an average number (73.54 x 108 CFU/ml). (Hand sanitizer concentration 25%) average amount (56.8 x 108 CFU/ml). (Handsanitizer concentration 30%) average amount (46.7 x 108 CFU/ml), (Handsanitizer concentration 35%) average amount (42.5 x 108 CFU/ml). Data analysis showed that based on the Anova test, the results showed that there was an influence of megkudu leaf extract hand sanitizer on the growth of Staphylococcus aureus. In the post hoc test, it was found that hand sanitizer with a concentration of 35% was the best concentration in inhibiting bacterial growth.
A POTENSI ANTIKOAGULAN EKSTRAK MENGKUDU ( MORINDA CITRIFOLIA L. ) DENGAN METODE LEE WHITE DAN APUSAN DARAH Kadiwanu, Merlin Rambu
Jurnal Sains dan Teknologi Laboratorium Medik Vol 10 No 2 (2024): November 2024
Publisher : Akademi Kesehatan John Paul II Pekanbaru

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Abstract

Mengkudu (Morinda citrifolia L.) dikenal mengandung senyawa kumarin yang berpotensi sebagai antikoagulan untuk mencegah pembekuan darah. Penelitian ini bertujuan menguji potensi antikoagulan ekstrak mengkudu melalui metode Lee White dan apusan darah. Metode ini dilakukan pada 15 sampel darah yang ditambahkan ekstrak mengkudu dengan konsentrasi 0,128 g dan 0,192 g. Waktu pembekuan diukur menggunakan metode Lee White, sementara perubahan morfologi darah diamati melalui apusan darah. Hasil penelitian menunjukkan bahwa ekstrak mengkudu konsentrasi 0,128 g menghasilkan waktu pembekuan rata-rata 11 menit 35 detik, sedangkan pada konsentrasi 0,192 g waktu pembekuan meningkat menjadi rata-rata 14 menit 37 detik. Uji statistik menunjukkan bahwa peningkatan waktu pembekuan pada kedua konsentrasi ekstrak mengkudu signifikan (p > 0,05), yang mendukung hipotesis bahwa ekstrak mengkudu memiliki efek antikoagulan. Kesimpulannya, ekstrak mengkudu memiliki potensi sebagai agen antikoagulan yang dapat memperpanjang waktu pembekuan darah, sehingga berpotensi digunakan sebagai alternatif antikoagulan alami untuk terapi pembekuan darah.
PENGARUH VARIASI LAMA PENYIMPANAN LARUTAN TURK MODIFIKASI AIR PERASAN BELIMBING WULUH (Averrhoa Bilimbi) TERHADAP HITUNG JUMLAH LEUKOSIT Rizky, Vionica Dwi; Sari, Putri Kartika; Cholijah, Dina Noor
Jurnal Sains dan Teknologi Laboratorium Medik Vol 10 No 2 (2024): November 2024
Publisher : Akademi Kesehatan John Paul II Pekanbaru

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52071/jstlm.v10i2.152

Abstract

Asam sitrat banyak terkandung dalam buah seperti jeruk nipis, jeruk purut, belimbing wuluh, nanas, pir dan buah lainnya. Selain mengandung asam sitrat belimbing wuluh juga mengandung asam askorbat. Asam sitrat tergolong asam lemah yang dapat melisiskan sel darah selain leukosit, dan dapat digunakan sebagai pengganti komposisi larutan turk. Penelitian ini bertujuan untuk mengetahui apakah perasan belimbing wuluh efektif sebagai pengganti komposisi larutan turk. Jenis penelitian ini menggunakan desain quasi eksperimen (Quasi experimental design) dengan menggunakan rancangan randomized posttest only control group design. Digunakan 12 replikasi dengan 1 spesimen yang sama pada setiap perlakuan. Penelitian ini menggunakan 2 variabel yaitu larutan turk pabrikan sebagai control dan larutan turk modifikasi air perasan belimbing wuluh sebagai eksperimen. Metode penelitian ini menggunakan desain deskriptif, dengan populasi 1 orang Mahasiswa Analis Kesehatan angkatan 2021 Universitas Borneo Lestari. Sampel 1 darah Mahasiswa, menggunakan teknik total sampling. Variabel adalah modifikasi air perasan belimbing wuluh (Averrhoa Bilimbi). Penelitian menggunakan instrumen berupa observasi laboratorium. Pengolahan data menggunakan editing, coding, dan tabulasi data. Analisa data berupa analisis deskriptif. Hasil jumlah leukosit yang diperoleh dari perhitungan rata-rata larutan Turk kontrol (6.487), modifikasi air perasan belimbing wuluh (Averrhoa Bilimbi) H1 (5.837), H7 (5.391), dan H14 (4.608). Kesimpulan dari penelitian ini pada larutan turk modifikasi air perasan belimbing wuluh (Averrhoa Bilimbi) pada H1 merupakan konsentrasi paling efektif dengan perbandingan hasil yang mendekati jumlah leukosit pada larutan kontrol, dan dapat digunakan sebagai pengganti komposisi larutan Turk.Kata-kata kunci: belimbing wuluh, pengaruh lama penyimpanan, desain deskriptif, jumlah leukosit, H1 konsentrasi paling efektif.

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