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MARINA CHIMICA ACTA

mca
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Published by Universitas Hasanuddin

ISSN : 14112132 EISSN : 26206145 DOI : -

Core Subject : Science,

Biochemistry, Genetics & Molecular Biology

When the first time appeared in 1999, Marina Chimica Acta was introduced as trilingual journal meaning that we can accept to publish articles in any kind of three languages such as bahasa Indonesia, English, and French. Starting April 2017, however, the publication has been made in English only, no more in bahasa Indonesia nor in French. Currently, Marina Chimica Acta is an international medium and occasional reviews of the publications of original studies (research papers) and occasional reviews in the field of marine chemistry. The journal will endeavor to cover all aspects, from chemical processes to theoretical and experimental work, and by providing a central channel of communication, to speed the flow of information in rapidly expanding disciplines. Submitted paper should be written in English.

Arjuna Subject : -

Articles 83 Documents

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DETERMINATION OF AGE OF CORAL REEFS ON PANIKIANG ISLAND, BARRU DISTRICT, SPERMONDE ISLANDS THROUGH MEASUREMENT ACTIVITY 14C USING LSC (LIQUID SCINTILLATION COUNTING) METHOD Arisandi Arisandi; Alfian Noor; Maming Maming
Marina Chimica Acta Vol. 20 No. 1 (2019): Volume 20, No. 1 (2019)
Publisher : Hasanuddin University

Determination Of Age Of Coral Reefs On Panikiang Island, Barru District, Spermonde Islands Through Measurement Activity 14C Using LSC (Liquid Scintillation Counting) Method. This research was using a sample of coral reefs taken from the Panikiang Island, Barru, Spermonde Islands. This research has been carried out by using sample preparation with physic and chemical. Sample preparation was done physically and chemically. Chemical preparation was carried out by using a mixture of NaOH with 30% H2O2 followed by a mixture of HClO4 with 30% H2O2, and finally with HCl solution to produce a clean sample with a weight reduction of 7.94%. Absorption of CO2 through reaction with HCl 10% and NH4OH as Carbosorb .produced K2CO3. The total carbon in the sample solution is 0.2496 gram sobtained through titration method. Radiocarbon dating method based on the measurement of the specific activity of the samples obtained from the results of counts LSC (Liquid Scintilation Counter) Hidex 300 SL.The specific activity of coral sample is 14.68 ± 4.04 DPM/gC. Age of coral sample which were calculated from the specific activity were 342.04 ± 37.66 years.

PRODUCTION OF PROTEASE ENZYME FROM BACTERIA IN HOT SPRING OF SOUTH SULAWESI, Bacillus licheniformis HSA3-1a Irwan R; Hasnah Natsir
Marina Chimica Acta Vol. 16 No. 1 (2015): Enzyme from Bacteria and Coral Age
Publisher : Hasanuddin University

Bacillus licheniformis HSA3-1a is able to produce extracellular protease. The aim of this research was to find out its production optimum time, consentration of MnCl2, temperature and optimum pH, also the cofactor influence on activity protease from B. licheniformis HSA3-1a. The protease activity was tested with a modified method of Walter. Protease production time was optimum for 36 hours with an activity value of 0,394 U/mL, protein content was 132,35 mg/mL. The Optimum condition of protease enzyme were pH 7,0 and temperature 45 ⁰C with an activity value of 0,0463 U/mL. Protease activity could be inhibited by MnCl2 concentration of 0,01 - 0,03 M.

THE POTENTIAL OF Clathria reinwardtii as BIOACCUMULATOR OF HEAVY METAL Cu Lydia Melawaty; Alfian Noor; Tjodi Harlim; Nicole de Voogd
Marina Chimica Acta Vol. 15 No. 2 (2014): Metals in Marine Samples and Age Determination using Liquid Scintillation Coun
Publisher : Hasanuddin University

Heavy metal Cu is an essential metal required by living organisms but a pollutant in water environment when existed in a level above the treshold level. The capability of sponge Clathria reinwardtii in accumulating heavy metals was analyzed according to modified Müller et al. (1998) method, and the sediment was analyzed according to Loring and Rantala in Rochyatun et al., (2006). Cu concentrations were measured by ICP-OES. The results indicate that Clathria reinwardtii was capable of accumulating the highest Cu level compared to other sponges. The highest Cu level analyzed in Clathria reinwardtii was 161.3 mg/kg.

ANALYSIS OF DIMETHYLSULFIDE AS BIOGEOCHEMICAL CYCLES SULFUR ON REGIONAL ESTUARY SPERMONDE Rakhmad Armus; Alfian Noor; Ahyar Ahmad; Muhammad Lukman
Marina Chimica Acta Vol. 19 No. 1 (2018): Volume 19, No. 1 (2018)
Publisher : Hasanuddin University

We developed a more accurate method of analysis for low-concentration volatile compounds used for the determination of free dimethylsulfide (DMS) in marine phytoplankton by using solid phase (SPME) gas chromatography - spectrometry Mass of GC-MS and head space method. DMSP, as a product of bacteria degradation has been scientifically recognized to be affected by climate change due to increased in sea surface temperature, and form DMS compunds.. The present study is to determine the DMSP concentration and degradation pathway in the oligotrophic region of Spermonde waters. The spatial and temporal correlation between DMSP degrading bacterial codes and biological potential controls for DMS formation in the open seas was examined using a statistical factorial analysis of variance and regression against the spatial (or distance) gradients of the Spermonde coastal waters, the concentration of chlorophyll-a, and diatom community structures. Seawater chemical properties, chlorophyll-a, and diatom samples were collected from several sites in the Tallo-Makassar river estuary, and the Pangkep river in September 2017 (transitional wet-to-dry-season). The result shows that the concentration of the Domination Index was higher in the transition season of 35.2 to 85.2 μM than in other seasons. The abundance of diatoms during the transition season reached 16,534 plankter / mL.

ANTIOXIDANT ACTIVITY AND TOXICITY POLYSACCHARIDE EXTRACT FROM RED ALGAE Eucheuma cottonii AND Eucheuma spinosum Muawanah Muawanah; Ahyar Ahmad; Hasnah Natsir
Marina Chimica Acta Vol. 17 No. 2 (2016): Volume 17 No. 2 (2016)
Publisher : Hasanuddin University

Red algae Eucheuma cottonii and Eucheuma spinosum which one alternative source of antioxidant and anticancer because contains polysaccharide compound. This research aims to isolate and examine the activity of antioxidant and toxicity polysaccharide extract from red algae Eucheuma cottonii and Eucheuma spinosum . The study was carried out by isolating the polysaccharide extract using water and methanol-ethanol precipitation. Antioxidant activity of crude extract was examined using 1,1-Difenil-2-pikrilhidrazil (DPPH) method, and toxicity test was carried out using Brine Shrimp Lethality Test (BSLT). The research results showed that the crude extract polysaccharide for Eucheuma cottonii and Eucheuma spinosum have strong antioxidant activity with IC50 value of 72,49 ppm and 75,98 ppm. The result of BSLT assay showed that the crude extract polysaccharide has a highest toxicity with LC50 value of 165,88 ppm and 337,21 ppm there are classified as toxic. The crude extract polysaccharide Eucheuma cottonii and Eucheuma spinosum has a potential to be developed as an alternative antioxidant and anticancer agent.

PURIFICATION, ANTIOXIDANT ACTIVITY AND TOXICITY POLYSACCHARIDE FRACTION FROM RED ALGAE Gracilaria verrucosa Muawanah Muawanah; Ahyar Ahmad; Hasnah Natsir
Marina Chimica Acta Vol. 18 No. 2 (2017): Volume 18 No. 2 (2017)
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/mca.v18i2.2688

it contains polysaccharide compound. This research aims to isolate and examine the activity of antioxidant and toxicity of polysaccharide extract and fraction fromred algae Gracilariaverrucosa. The study was carried out by isolating the polysaccharide extract using aqueous 100 o C and methanol-ethanol precipitation .Purification of polysaccharide done by Column Chromatography DEAE-Cellulose and analysis polysaccharide concentration was using phenol-sulfuric acid. Antioxidant activity of crude extract and polysaccharide fraction was examined using DPPH method, and toxicity test was carried out using Brine Shrimp Lethality Test (BSLT). The research results reveals that the highest polysaccharide concentration was fraction 7 with value of 0,267 mg/mL, and the crude extract and fraction 4 have strongest antioxidant activity with IC50 values of 51,22 and 62,25 ppm, respectively. The result of BSLT assay showed that the crude extract and fraction 6 has a highest toxicity with LC50 values of 47,73and 58,48 ppm, therefore it is classified as toxic. The polysaccharide of crude extract and fraction 7 has a potential to be developed as an alternative antioxidant and antitumor agent. Identification with FT-IR shows the characteristic bands of agarocolloid.

UTILIZATION OF SODIUM HYDROXIDE AS ABSORBER FOR 14C ANALYSIS Febby Kurniaty; Maming Maming; Muhammad Zakir; Alfian Noor
Marina Chimica Acta Vol. 16 No. 1 (2015): Enzyme from Bacteria and Coral Age
Publisher : Hasanuddin University

Research on utilization of sodium hydroxide as absorber for 14C analysis of coral ages estimation in Langkai Island using LSC (Liquid Scintillation Counting) method has been done. Samples analyzed comes from Langkai Island, which is relatively low disturbing by human activities. Samples were cleaned according to physical and chemical treatment using mixture of acid-base solution: NaOH , H2O2 , HClO4 and HCl. This step remove about 8,62% mass of the sample and it leaves white. Carbonated of sample were remove as CO2 by reaction with HCl 10% and trapped by 5M NaOH as Na2CO3. Total carbon in the sample can be determined by titration method with the total weight of carbon sample are 0,2688 g / 8 mL. Specific activity of carbon-14 in the sample that were counted by Hidex 300 SL liquid Scintillation Counter were 14.72 ± 7.92 DPM/gC related to their age. By applying specific activity of the samples and specific activity carbon-14 in the modern carbon (15.3 ± 0.1 DPM/gC) to radioisotop desintegration equation, the age were 316,90±170,506 years.Keywords : Carbon Dating, Liquid Scintillation Counting, Coral, Spermonde Archiphelago

DIETHANOLAMINE AS CO2 ABSORBENT FOR 14C ANALYSIS TO DETERMINATION AGE OF CORAL REEF FROM PANAMBUNGAN ISLAND BY USING LIQUID SCINTILLATION COUNTING (LSC) METHOD Seniati Salahuddin; Alfian Noor; Muhammad Zakir
Marina Chimica Acta Vol. 18 No. 1 (2017): Volume 18, No. 1 (2017)
Publisher : Hasanuddin University

Utilization of diethanolamine (DEA) as an absorbent for 14C analysis using liquid scintillation counting (LSC) on the determination of the age of the coral reefs have been done. Coral reefs are analyzed from the uninhabited Panambungan island which far away from the influence of human activity. Sample preparation, physical and chemical by using 2 acid-base mixture: H2O2-NaOH and HClO4-HCl. At this stage there is a weight reduction of as much as 9.023 gram sample. Carbonate samples reacted with HCl to separate CO2 and sequestration by diethanolamine into carbamate compounds. Total carbon in the sample solution is obtained through the reduction of 0.838 grams before and after absorption. The specific activity of 14C in samples measured by the counter LSC HIDEX 300 SL and obtained 8.4026 ± 6,85 DPM/gC. The specific activity of the sample and the specific activity of modern carbon (15.3 ± 0.1 DPM/gC) is inserted into the equation radioisotope decay, obtained the age of 4955 ± 935 years.

ANALYSIS OF THE LEAD (Pb) CONTENT IN SEDIMENT, SEA WATER AND BLOOD COCKLE Anadara granosa L. FROM THE COASTAL WATER OF MAKASSAR Olivia Datu Parung; Magdalena Litaay; Eva Johannes
Marina Chimica Acta Vol. 16 No. 2 (2015): Volume 16 No. 2 (2015)
Publisher : Hasanuddin University

The research about analysis of the content of heavy metal lead (Pb) in sediment, sea waterand blood cockle Anadara granosa L. from the caostal water of Makassar has been done.This research was aimed to figure out the Pb content in the sediment, the sea water and theblood cockle A. granosa L. The sample was taken by using Purposive sampling method. Thesamples preparation used composite method and Pb level in samples was determined byAtomic Absorption Spectrofotometric (AAS). The result showed that at Tanjung Bunga, theaverage of Pb content in the: sea water 0,289 ppm, sediment 0,609 ppm and blood cockle A.granosa L. 0,704 ppm. In the Mangara Bombang, the average of Pb content in the sea water0,273 ppm but Pb level in the sediment and in the blood cockle A. granosa L. were notdetected.

Heavy Metal Concentrations of Chromium (Cr) and Copper (Cu) Based on Particle Size and Sediment Depth Variation in Paotere Port Waters, Makassar Rizky Febrina Irwan; Alfian Noor; Maming Gaffar
Marina Chimica Acta Vol. 14 No. 2 (2013): Volume 14 No. 2 (2013)
Publisher : Hasanuddin University

Analisis kandungan logam berat Cr dan Cu berbagai ukuran partikel sedimen di perairan Pelabuhan Poatere Makassar telah dilakukan. Sampel sedimen diambil dengan menggunakan pipa polietilen yang panjangnya 30 cm. Sampel diayak menggunakan ayakan mekanik ukuran 1000, 250 dan 63 µm. metode analisis yang digunakan adalah AAS (Atomic Absorption Spectroscopy) yang diawali dengan penambahan HNO3 lalu didestruksi menggunakan microwave. Sebaran ukuran partikel sedimen permukaan pada perairan Pelabuhan Paotere yaitu untuk ukuran partikel 250 – 1000, 63 – 250 dan 63 µm berturut-turut adalah 36,04%; 29,51% dan 10,07%. Ukuran partikel sedimen berbanding terbalik dengan konsentrasi logam berat. Konsentrasi Cu dalam sedimen berukuran 63 µm adalah 141,55 mg/kg; 250 µm adalah 56,52 mg/kg dan 1000 µm adalah 51,50 mg/kg. Konsentrasi Cr dalam sedimen berukuran 63 µm adalah 36,68 mg/kg; 250 µm adalah 18,65 mg/kg dan 1000 µm adalah 8,78 mg/kg. Konsentrasi logam semakin kecil seiring dengan bertambahnya kedalaman sedimen. Konsentrasi Cu dalam sedimen kedalaman 0 - 10 cm adalah 93,46 mg/kg; 10 - 20 cm adalah 77,51 mg/kg dan 20 - 30 cm adalah 76,28 mg/kg. Konsentrasi Cr dalam sedimen kedalaman 0 - 10 cm adalah 22,04 mg/kg; 10 - 20 cm adalah 21,68 mg/kg dan 20 - 30 cm adalah 20,87 mg/kg.

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