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Berkala Penelitian Hayati
Published by Perhimpunan Biologi Indonesia Cabang Jawa Timur
ISSN : 08526834     EISSN : 2337389X     DOI : https://doi.org/10.23869/bphjbr
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology
Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by Ministry of Culture and Education. It continues recorded by Zoological Record by Thomson Reuters Clarivate Analytics since 2011. Since April 2012, the journal was changed into English. This journal is indexed by DOAJ, Crossref, Google Scholar, Academia.edu, and EBSCO Host. This journal publishes original research, applied, review article, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. This journal publishes original research, applied, review articles, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. The journal scopes include, but are not limited to, the following topic areas including botany, zoology, ecology, microbiology, physiology, nanobiology, coastal biology, hydrobiology, neurobiology, genetics, developmental biology, biochemistry and molecular biology, biophysics, and life science.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 15 Documents
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Search results for , issue "Vol 16 No 2 (2011): June 2011" : 15 Documents clear
KOMPOSISI KIMIA DAN UJI AKTIVITAS ANTIBAKTERI MINYAK KEMANGI (Ocimum americanum L.) TERHADAP BAKTERI Escherichia coli, Shigella sonnei, DAN Salmonella enteritidis Asep Kadarohman; Gebi Dwiyanti; Yuni Anggraeni; Lela Lailatul Khumaisah
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 16 No 2 (2011): June 2011
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/287

Abstract

The chemical composition and antibacterial activity of basil oil (Ocimum americanum L.) against Escherichia coli, Shigella sonnei and Salmonella enteritidis have been determined. Basil oils were isolated from leaves and stems of basil (Ocimum americanum L.) with percolation method and analyzed by FTIR and GC-MS spectrometry. The antibacterial activity of basil oils were obtained by Agar diffusion method with various concentration (v/v) 2, 4, 6, 8, and 10% with ethanol p.a. as negative control, thiamfenicol, and tetracycline 500 mg as positive control. The produces essential oils from leaves and stems of basil by percolation are 1.06 and 0.22%, respectively. There are 22 components of basil oil were identified, with a major component are citral (35.58%) and neral (29.56% ). Basil oil has not effectively against Escherichia coli and Shigella sonnei but effectively against Salmonella enteritidis at concentration 8 and 10%, with inhibition zone diameter are 10.25 and 10.93 mm respectively.
EKOSISTEM TERUMBU KARANG DAN KONDISI OSEANOGRAFI PERAIRAN KAWASAN WISATA BAHARI LOMBOK Muhlis
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 16 No 2 (2011): June 2011
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/288

Abstract

The research that was carried out in Lombok marine tourism is purposed to find out 1) the condition of coral reef ecosystem 2) the condition of waters oceanography and 3) the relationship between the conditions of coral reef ecosystem and the oceanography condition of Lombok marine tourism. The observation was conducted with the line transect method in the windward zone and leeward zone, in 3 meters and 10 meters depth with three test times. The condition of coral reef that would be analyzed is about 1) percentage of the coral cover 2) the death index of coral 3) the form of growth coral, whereas the oceanography condition that would be analyzed is the temperature, salinity, the pH, DO, the brightness, and the flow speed. Knowing the relationship between the condition of coral reefs and the condition of oceanography will be carried out with the analysis of regression. The result shows that the condition of coral reefs ecosystem in windward zone has 36.9% cover coral, the death index of coral 0.17, the form growth coral that was found is 13 kinds 251 number of forms of the growth coral, while the condition of coral reef ecosystem in leeward zone reached 23.72% cover coral, the death index of coral 0.45, the form of growth coral that was found is 11 kinds 276 number of forms of the growth coral. The condition of oceanography factor of windward zone has 22,36 meters brightness, the temperature 27.52◦C, the flow speed 4.77 m./sec, the salinity 33.21 Ppt, the pH 7.56 and the protracted oxygen 6.41 Mg.l-1, the brightness of leeward zone reached 14.36 meter, the temperature 27.83◦C, the speed of the flow 2.68 m./sec, the salinity 31.54 Ppt, the pH 7.77 and the protracted oxygen 5.40 Mg.l-1. There is relationship between the oceanographic factor and the condition of coral reef ecosystem, from the sixth oceanographic factors, salinity has the highest effective contribution that is 49.79%, and the lowest is the pH that only 1.52%.
POLA PEWARISAN SIFAT DAYA HASIL KACANG TANAH HASIL PERSILANGAN cv. KELINCI DAN US 605 DALAM KONDISI TERCEKAM KEKERINGAN Adisyahputra; Sudarsono; K. Setiawan
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 16 No 2 (2011): June 2011
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/289

Abstract

The aim of this research was to examine the yield characters of peanut in drought stress. The experiment was conducted by using of peanut cv. Kelinci that is sensitive genotype as female parent and US 605 which is tolerant genotype as male parent, including the F2 population F1 offspring from hybrid cv. Kelinci (P1) with US 605 (P2). The number of pods, amount of filled pods, dry weight of pods, dry weight of seed, the number of seeds, are measured during harvest. Expression of all yield characters were influenced by genetic factor, except the variable weight of seed. All of yield show the quantitative and qualitative characters. The result analysis of Mendelians genetic yield showed all characters were controlled by 2 until 3 gene with the dominant and resesif epistasis combination pattern. Result from joint scalling test analysis showed all variable were not only determined by additive and dominant influence but also by genetic interaction. Additive influence with the high narrow sense heritability most of all variables together can give opportunity to obtain tolerant peanut line with heigh yield.
SKRINING UNTUK TOLERANSI TERHADAP STRES KEKERINGAN PADA 36 VARIETAS KEDELAI PADA FASE PERKECAMBAHAN Wahyu Widoretno
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 16 No 2 (2011): June 2011
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/290

Abstract

Water stress due to drought is the most significant abiotic factor limiting crop growth and development. Drought stress inhibit germination and seedling growth, and extention of inhibition in germination under drought stress condition is varying in varieties. The previous research result showed that the differences in germination response under drought stress can be used as early test for screening to drought tolerance in plants. The objectives of this experiment were to evaluate the germination response of 36 soybean varieties under drought stress simulated by polyethylene glycol (PEG), to know the drought-stress tolerance level of 36 soybean varieties based on germination response in medium containing PEG, and to identify drought tolerant varieties. Soybean seeds were germinated in sand medium containing several PEG (6000) at concentration 0%, 15% and 20% which equivalent to 0, -0,41 and -0,67 MPa during 5 days. Germination responses were observed by calculating vigor index, germination percentage, lateral root number and measuring hypocotyl and root lenght and seedling dry weight. The level of drought stress tolerance of soybean varieties were evaluated based on index of reduction and drought sensitivity on germination variables in stres and non-stres conditions. The research result showed that drought stress on germination media simulated by PEG decreased vigor index, germination percentage, lateral root number, hypocotyl and root lengths and dry weight of seedling. The inhibition of germination increased with reduction of water potential. The water potential -0,41 MPa inhibited vigor index and hypocotyls length in all tested varieties, but inhibited germination percentage, lateral root number and dry weight of seedling in some varieties. However, decreasing of water potential -0,67 MPa inhibited almost germination variables in all varieties, except root length. Each tested soybean variety showed differences of germination response to drought stres. Varieties of Dieng, Tidar and Sibayak showed less inhibition than others, but Anjasmoro, Burangrang, Galunggung, Kipas Putih and Tambora showed more inhibition in seedling growth. Based on index of reduction and drought sensitivity on germination variables, Dieng and Tidar were identified as tolerant varieties, while sixteen varieties as medium tolerant and seventeen varieties as sensitive ones.
POTENSI PHOTODINAMIK INAKTIVASI Staphylococcus aureus DAN Vibrio cholerae DENGAN ENDOGEN PHOTOSENSITIZER PADA PENYINARAN LED BIRU (430 ± 4) nm DAN MERAH (629 ± 6) nm Suryani Dyah Astuti; Djoni Izak R.; Ni’matuzahroh; M. Zainuddin; Suhariningsih
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 16 No 2 (2011): June 2011
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/291

Abstract

Photodynamic Inactivation (PDI) is bacteria inactivation method with using light and bacteria porphyrin photosensitizer.The combination of light and photosensitizer with suitable spectrum can promote photosensitization process and then cause bacteria photodamage. This research was laboratory experiment, to analyze photodamage potency of Gram positive bacteria Staphylococcus aureus and Gram negative Vibrio cholera with combination of endogen photosensitizer and LED exposure (blue LED (430 ± 4) nm and red LED (629 ± 6) nm)) on Pulse Width Modulation (PWM) 75% and time duration 30 minutes. The viability of bacteria had been counted after 48 hours incubation on temperature 37oC by using Total Plate Count (TPC). Result of this research showed that blue LED exposure (430 ± 4) nm had potency to decrease 70% Staphylococcus aureus and 50% Vibrio cholera bacteria colony forming unit. Red LED (629 ± 6) nm exposure decreased 22% dan 3% colony forming unit. So blue LED exposure had big potency to bacteria inactivate.
DIVERSITAS GASTROPODA DI SUNGAI SUKAMADE, TAMAN NASIONAL MERU BETIRI, JAWA TIMUR Putut R. Purnama; Nimas W. Nastiti; Melia E. Agustin; Moch. Affandi
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 16 No 2 (2011): June 2011
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/299

Abstract

Sukamade River is one of the main rivers in Meru Betiri National Park, especially at Sarongan Resort. Macrofauna benthic community in this river are in the protected areas that allegedly still original, and has not got the impact of human activity. Information about the diversity of gastropods class in Sukamade River in limited encourage to do research on the diversity of gastropods in this place. This research was conducted to determine the diversity of gastropods in Sukamade River. Gastropods samples taken at five stations those are in estuary, middle, and part of the river that closer to upstream river using surber-net and hand net. Surber-net used to take samples in watersheds zones and riparian zones using hand net. Samples were obtained, inserted the container that contains the fixative solution that formed by formalin 6% for preservation. Samples that have been preserved, and then sorted, identified and counted the number of individuals of each species, and data analysis. Data analysis, among others, to calculate the diversity index, evenness index, and dominance index in the Laboratory of Ecology Department of Biology, Airlangga University. From the analysis, obtained by the number of species found are 20 species that range from 8–14 species per station, the diversity index ranged from 1.860 to 0.795, evenness index ranged from 0.383 to 0.723 and the dominance index ranged from 0.223 to 0.659. Can be concluded that the diversity of gastropods in Sukamade River is at low-medium category.
EFFECTS OF BIOFERTILIZER CONTAINING MICROBIAL OF N-FIXER, P SOLUBILIZER AND PLANT GROWTH FACTOR PRODUCER ON CABBAGE (BRASSICA OLERACEAE VAR. CAPITATA) GROWTH AND SOIL ENZYMATIC ACTIVITIES: A GREENHOUSE TRIAL Sarjiya Antonius; Dwi Agustiyani
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 16 No 2 (2011): June 2011
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/301

Abstract

Biofertilizer has been identified as an alternative to chemical fertilizer to increase soil fertility and crop production in sustainable farming. The objective of this greenhouse study was to evaluate the effects of four different concentrations of biofertilizers containing Pseudomonas sp., Bacillus sp. and Streptomyces sp. on soil properties and to evaluate the growth of Brassica oleraceae var. capitata. The application treatments included control (no fertilizer) and four concentration of diluted biofertilizer per pot (20 ml, 40 ml, 60 ml and 80 ml). The application of biofertilizer containing benefi cial bacteria signifi cantly increased the growth of B. oleraceae. The use of biofertilizer resulted higher biomass weight and length as well as root length. This greenhouse study also indicated that different amount of biofertilizer application had almost similar effects. Microbial inoculum not only increased plant harvest, but also improved soil properties, such as number of microorganisms, respiration and urease activities.
PERUBAHAN AKTIVITAS ENZIM LIPASE DARI BUAH KELAPA SELAMA PERTUNASAN Moh. Su'i; Harijono; Yunianta; Aulani'am
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 16 No 2 (2011): June 2011
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/302

Abstract

This research learned about lipases activity from coconut during germination. Coconut was growth for 0, 15, 30, 45 and 60 days in the darkplace and rate temperature. Shoot, houstorium, radicless and endosperm were taken and then they were measured weigh and long. Lipases was extracted from each of them and measured volume and activity. The results of research showd that highest lipases activity was in shoot that were grew for 45 days with enzyme activity was 0,060 unit/mg protein.
MORFOGENESIS PADA DAUN, TANGKAI DAUN, DAN RUAS BATANG KENTANG HITAM (Solenostemon rotundifolius (POIR) JK MORTON) SECARA IN VITRO Aryani Leksonowati; Witjaksono
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 16 No 2 (2011): June 2011
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/303

Abstract

Solenostemon rotundifolius (Poir) JK Morton is a minor crop that produce carbohydrate and therefore can be utilized for food diversifi cation and help food security program. This crop is not cultivated intensively but it is planted as a side crop during the dry season. Low yield could be responsible for its unattractiveness for farmer to farm it intensively. Improvement of varieties is needed but it is hindered by the low genetic variability due to lack of sexual reproduction. Manipulation at cell level and biotechnology could be an alternative for improving this crop. The availability of a reliable protocol(s) for plant regeneration is needed. This study is intended to develop reliable regeneration protocols using inocula derived from shoot culture on MS basal medium supplemented with various level of BA and NAA. The results show that inocula of leaf, petiole, and stem internodes respond classically to the varying concentration of sitokinin BA and auxin NAA. The optimum production of shoot that refl ect the best shoot organogenic response were achieved by leaves inoculums grew on medium supplemented with 5 mg/l BA and 0.1 mg/l NAA. This protocol serves as a protocol for further somatic cell genetic experiments to increase genetic variability of this crop.
BIOPRODUKSI FLOROGLUSINOL OLEH JAMUR ENDOFIT COELOMYCETES AFAS-F3 YANG DIISOLASI DARI TUMBUHAN Archangelesia flava L. MERR. Yuliasri Jamal; Praptiwi; Ahmad Fathoni; Andria Agusta
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 16 No 2 (2011): June 2011
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/304

Abstract

Isolation and identification of secondary metabolites of endophytic fungus associated with the plant of Archangelisia flava Merr. and antibacterial activity test were done in this study. Extraction of biomass of endophytic fungus culture and its secondary metabolites isolation were done in ethyl acetate and methanol solvent then followed by thin layer and colomn chromatography. The fractions from colomn chromatography were tested for antibacterial activity against Escherichia coli by paper disc method. The result showed that the extract composed of 9 fractions. Fraction 4th and 8th had growth inhibition zone, 8 mm and 10 mm respectively, against E.coli at the concentration of 100 mg/ml. Isolation and purifi cation process through several chromatography techniques yielded one major metabolites wich was identifi ed as phloroglucinol by co-chromatography technique on a GC-MS machine. Phloroglucinol can produce 10 mm clear zone on a disc antibacterial assay against E. coli.

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