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INDONESIA
Berkala Penelitian Hayati
Published by Perhimpunan Biologi Indonesia Cabang Jawa Timur
ISSN : 08526834     EISSN : 2337389X     DOI : https://doi.org/10.23869/bphjbr
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology
Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by Ministry of Culture and Education. It continues recorded by Zoological Record by Thomson Reuters Clarivate Analytics since 2011. Since April 2012, the journal was changed into English. This journal is indexed by DOAJ, Crossref, Google Scholar, Academia.edu, and EBSCO Host. This journal publishes original research, applied, review article, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. This journal publishes original research, applied, review articles, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. The journal scopes include, but are not limited to, the following topic areas including botany, zoology, ecology, microbiology, physiology, nanobiology, coastal biology, hydrobiology, neurobiology, genetics, developmental biology, biochemistry and molecular biology, biophysics, and life science.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 6 Documents
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Search results for , issue "Vol 20 No 2 (2015): June 2015" : 6 Documents clear
THE PREVALENCE OF H5N1 INFLUENZA VIRUS ON POULTRY AT TRADITIONAL MARKET IN SEMARANG, INDONESIA R. Susanti
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 20 No 2 (2015): June 2015
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (321.102 KB) | DOI: 10.23869/101

Abstract

Traditional market is a potential source of the spread of avian influenza virus. This research is aimed to determine the prevalence of avian influenza virus (AIV) subtype H5N1 on poultry sold in traditional market in Semarang City. Fifty-five poultry samples sold in six traditional markets in Semarang City; i.e. Karangayu market, Mangkang market, Gunungpati market, Rejomulyo market, Gayamsari market and Karimata market taken from its cloaca swab. Further, cloacal swab samples grown in pathogen-free chicken embryos aged nine days. Then, it incubated for four days at 37 °C. Allantoic fluid then collected and tested to agglutinate red blood cells. RNA extracted from the samples showed haemagglutination activity. Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) applied to identify the subtype of AIV using the specific primer H5 and N1. The PCR product resulted from RT-PCR then visualized using electrophoresis. The results showed that four AIV subtype H5N1 isolates have successfully isolated from cloacal swab samples (7.27%) with the distribution of 2 isolates were from Mangkang Market (28.57%), 1 isolate was from Rejomulyo Market (7.69%) and 1 isolate was from Karimata Market. The prevalence of AIV subtype H5N1 was 28.57% in Mangkang market, 7.69% in Rejomulyo market and 9.09% in Karimata market. The prevalence values of each species were 7.89% in chicken and 9.09% in the wild duck. According to this result, poultry sold in traditional market in Semarang have a potential source of AIV subtype H5N1.
RESISTANCE LEVEL OF SOME SOYBEAN (Glycine max L. Merr) GENOTYPES TOWARD SALINITY STRESS Runik Dyah Purwaningrahayu; Husni Thamrin Sebayang; Syekhfani Syekhfani; Nurul Aini
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 20 No 2 (2015): June 2015
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (542.166 KB) | DOI: 10.23869/103

Abstract

Salinity resistance level on soybean which influenced by its genotype is important to be explored.One of the important factors which determine the success of soybean cultivation on saline soil or salt-affected soil is salinity resistance level. Eleven variants or genotypes of soybean were collected from Indonesian Legumes and Tuber Crops Research Institute and were tested on four different electrical conductivity level of soil (0.5 dS/; 5.8 dS/m; 8.4 dS/m; and 12.2 dS/m). Soybeans were planted on pot in green house. Salinity stress will started when plants were on 21 days after planting (DAP). The treatment was watering using some different concentration of sea water for 30 days. Three methodswhich used to determine salinity resistance level of soybean were seed yield reduction level, stress susceptibility index (SSI), and leaf scorch score. Soybean will classified into salinity tolerant genotype group when weight reduction of seed was <66%, leaf scorch score was <2.0 and SSI was <0.95. Results showed that genotypes with 0.5-5.8 dS/mof soil electrical conductivity tolerance were: Wilis, Tanggamus, Gema, LK/3474-403, MLG 3474-991, IAC100/Burangrang//Malabar-10-KP-30-75, MLG 2805-962; genotypes with8.4 dS/m of soil electrical conductivity tolerance were SU-7-1014 and Argomulyo//IAC100-10-KP-40-120; and genotypes with 12.2 dS/m of soil electrical conductivity tolerance were IAC100/Burangrang//Malabar-10-KP-21-50 and Argopuro//IAC100. These results can be used by soybean breeder as a reference in improving salinity resistance level of soybean for further study as anticipation of salt affected soil wide spread.
THE PATTERNS OF SEX DETERMINATION AND DIFFERENTIATION GENES IN GREEN SEA TURTLE (Chelonia mydas) Anggraini Barlian; Noviana Vanawati; Fitria D. Ayuningtyas
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 20 No 2 (2015): June 2015
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (583.808 KB) | DOI: 10.23869/104

Abstract

Green sea turtle (C. mydas) is one of TSD (Temperature-dependent Sex Determination, TSD) animals which mean that their sex is determined by the egg’s incubation temperature. Genotypic Sex Determination (GSD) homologous genes play a role in TSD process. Until now, research on the pattern of sex determination genes in C.mydas has not been conducted yet. The aim of this research is to reveal sex determination and differentiation genes expression in Mesonephros-Gonad (MG) complexes of C. mydas embryos which incubated in masculinizing temperature (MT) and feminizing temperature (FT). C. mydas eggs were incubated in 3 different stage of TSP (Thermosensitive Period) at masculinizing temperature (26±10C, MT) and feminizing temperature (31±10C FT). Mesonefros-gonad complexes were isolated at Pre-TSP stage (FT at 14th day, MT at 24th day), TSP stage (FT at 24th day, MT at 36th day) and differentiated stage (FT at 40th day, MT at 58th day). RNA from mesonephros-gonad (MG) complexes were converted into cDNA by RT-PCR process. Pattern of Sf1, Wt1, Aromatase, FoxL2, Sox9, Wnt4, Fgf9 and Rspo1 genes expression were analyzed by quantitative Real Time PCR (qPCR) method with β-actin gene as an internal control. Result of this study shown that expression pattern of Sf1, Wt1, Aromatase, FoxL2, Sox9, Wnt4, Fgf9, and Rspo1genes in gonadal embryo of C. mydas were increased during gonadal development stage. Four genes expression patterns (Wnt4, Fgf9, Rspo1, and FoxL2) have shown that these genes have role in sexual differentiation rather than in sexual determination.
KENAF : ITS PROSPECT IN INDONESIA Estri Laras Arumingtyas
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 20 No 2 (2015): June 2015
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (318.682 KB) | DOI: 10.23869/105

Abstract

Kenaf is a plant fibre with high potential as source of material industry. Originally, kenaf usage in Indonesia is still limited only for jute sacks material, which is then displaced by plastic sacks production. While at international scale, kenaf has been started to be developed as pulp material, polypropylene composite, fibreglass replacement, and particle board for automotive industry materials. Indonesia is a tropical country this condition which suitable for kenaf cultivation. However, research reports about kenaf potential usages are still few and limited in domestic level only. Whereas, Indonesian kenaf plant information is needed by international community to understand comprehensively about the potential of tropical plants. This article aims to provide an overview about kenaf cultivation potential and usages in Indonesia as well as the possibility of future development.
TOBACCO DIVERSITY IN INDONESIA Djajadi Djajadi
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 20 No 2 (2015): June 2015
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (460.661 KB) | DOI: 10.23869/106

Abstract

Tobacco variants in Indonesia are very diverse which can be identified from their morphology or their characteristics. This is related to tobacco long adaptation in different agro ecology of plantation areas which spread out at 15 provinces, from dry to irrigated land and from low land to high land areas. Tobacco has been introduced in Indonesia for more than four centuries and mostly used as cigarette. This commodity and its products are still economi-cally important for government and farmer income. It contributes in government income which reached up to 114 trillion rupiahs and farmer income up to 70% in 2014. Tobacco diversity in Indonesia can be grouped according to their growing season and their usage in cigarette blending. Tobaccos which grown at the end of wet season and harvested in dry season are called Voor Oogst tobaccos, otherwise tobaccos which grown at dry season and harvested in wet season are called Na Oogst tobaccos. Based on their usage, tobaccos are categorized as main ingredients for kretek cigarette, Rolled Your Own (RYO) cigarette, and cigar industries.
Δ6 FATTY ACYL DESATURASE GENE IN A SOUTHERN BLUEFIN TUNA (Thunnus maccoyii) CELL LINE Senni Juniawati Bunga; Kathy Schuller
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 20 No 2 (2015): June 2015
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (495.923 KB) | DOI: 10.23869/107

Abstract

Fish is the main source of ω-3 long chain polyunsaturated fatty acids (LC-PUFA) such as eicosapentaenoic acid (EPA, 20:5ω-3) and docosahexaenoic acid (DHA, 22:5ω-3), which have positive effects on human health and can be beneficial in human diet context. Studies involving fatty acyl desaturase (Fads) and elongase of very long chain fatty acids (Elovl) enzymes that convert C18 PUFA to C20/22 LC-PUFA have been performed in some fish species. However, very little is known about LC-PUFA biosynthesis in tuna species. This study investigated the Δ6 Fads gene performance in the SBT cell line. The Δ6 Fads nucleotide sequences from various fish species were identified and retrieved to compare them with the SBT Δ6 Fads nucleotide sequence. The Δ6 Fads gene was performed using real time PCR (RT-PCR) and then was compared it with β-actin gene performance as a reference housekeeping gene. By performing multiple sequence alignments and comparing the highly conserved regions among fish Δ6 Fads sequences, the SBT Δ6 Fads nucleotide sequence was determined to be ≥ 75% identical to the other fish Δ6 Fads sequences. The results showed that when the SBT Δ6 Fads and β-actin cDNAs were performed in a standard PCR system and the products were analysed by electrophoresing them on a 2% (w/v) agarose gel, the target genes that were obtained were similar to the expected sizes. The observed band size for the Δ6 Fads PCR product was 207 bp and for the β-actin PCR product was 98 bp. The presence of the observed bands indicated that the primer pairs that were designed and used were successfully amplified the target genes. The results of this study might provide relevant information to support further investigating of the desaturase and elongase gene expression that might contribute to a better understanding of ω-3 LC-PUFA biosynthesis in fish.

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