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Jurnal Natur Indonesia

JN
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Published by Universitas Riau

ISSN : 14109379 EISSN : 25030345 DOI : https://doi.org/10.31258/jnat

Core Subject : Science, Agriculture, Engineering,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry

Jurnal Natur Indonesia published since 1998, is a science journal that presents articles on research results, thoughts and views from researchers and experts in the field of biosciences (basic science), including biology, physics, chemistry and mathematics. In the field of biosciences (basic science); Biology, Physics, Chemistry, Mathematics.

Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)

Articles 10 Documents

Search results for , issue "Vol 11, No 1 (2008)" : 10 Documents clear

Terjadinya Isomerisasi dan Oksidasi - dan -Karoten Selama Proses Pengolahan CPO Kusumaningtyas, Reni Subawati; Martosupono, artanto
Jurnal Natur Indonesia Vol 11, No 1 (2008)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Isomerization and oxidation has been known as the major factors of the degradation of carotenoid compounds.The carotenoid compounds in oil palm fruit are dominated by - and -karoten. Result of this experiment showedthat the isomerization of the - and -karoten during the process productions of crude palm oil (CPO) indicated bythe reducing of trans ? and trans -caroten concentrations and followed by the increasing of cis - and cis ?-caroten concentrations. Isomerization promote to increasing the concentration of cis - and cis -carotenapproximately 40 ppm and 19 ppm on the sterilization process, then 79 ppm and 134 ppm on the extraction andclarification process of crude oil. On the other hand, the purification process just only increased the concentrationof cis ?-karoten about 19 ppm. The Oxidation of the - and -caroten caused to the increasing of lutein andzeaxantin as the degradation product. The - and -caroten oxidation can be increasing the amount of lutein andzeaxantin approximately 1 ppm and 5 ppm on the sterile fruit, 22 ppm and 14 ppm on the extract crude oil, 1 ppmand 3 ppm on purifying oil. The oxidation process of oil palm extract in the vacuum drier tank can be increased thelutein concentration about 6 ppm.

Isolasi dan Karakterisasi Inulinase dari Aspergillus niger Gmn11.1 Galur Lokal Saryono, Saryono
Jurnal Natur Indonesia Vol 11, No 1 (2008)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Inulin is a naturally potential polysaccharide used to produced fructose and fructooligosaccharide. Inulinaseknown also as ß-fructosidase can hydrolise inulin to fructose or fructooligosaccharide. Inulinase production fromAspergillus niger Gmn11.1 isolated from dahlia tubers is conducted using medium containing 1% inulin and 0,2%yeast extract. The crude enzyme (filtrate culture) is purified by means of ammonium sulphate salt precipitation,followed by Sephadex G25 gel filtration column chromatography and DEAE cellulose anion exchanger columnchromatography. The result indicated that the enzyme had optimum pH and temperature of 4,6 and 450C, respectivelywith incubation time of 15 hours. The Km and Vmaxs values obtained from this experiment are 20 mg/ml and 0,769mg/ml/hours, respectively. Whereas the relative molecular weight of inulinase was monitored by SDS PAGE is 63KDa.

Efek Hepatoprotektif Ekstrak Buah Merah (Pandanus conoideus Lam.) pada Hati Mencit Jantan Galur Swiss induksi dengan CCl4 Nugraha, Ari Satia; Hadi, Ninisita Sri; Siwi, Sri Untari
Jurnal Natur Indonesia Vol 11, No 1 (2008)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

A research on red fruit (Pandanus conoideus Lam.) has been conducted to determine its hepatoprotective effect.This research was concern on three group of treatments, a blank treatment (water), a standard drug (curcuminecontaining) and red fruit extract treatment group. The liver destruction was induced by CCl 4. The hepatoprotectiveeffect was illustrated by SGOT – SGPT level of activity and percentage of cell destruction obtained from histopatogolicanalysis. Compared to the blank group, which had level of SGOT-SGPT activity as 38224,40  2,92 U/L and SGPT of24128,00  5,22 U/L, the red fruit treatment group showed a lower SGOT – SGPT activity (20112,4  2,68 U/L and18923,0  2,77 U/L, respectively); while the standard drug treatment group showed level of SGOT and SGPT activityas 29732,4  1,85 U/L and 20640,8  3,78 U/L, respectively. The histopatologic evaluation also illustrated similaritythat the red fruit treatment group occupied the lowest percentage of hepatocyte destruction shown as percentageof cell degeneration and cell necrosis of 28,3% and 31,7%, respectively. The standard drug treatment showed78,3% of destruction based on degenerative cell destruction and 88,3% based on cell necrosis. Almost 100% of celldestruction was shown in the blank group. Based on these result, the red fruit extract possessed a liver cellprotection activity against cell destruction caused by CCl4 exposure and even more active than a standard drug.

Isolasi dan Identifikasi Komponen Utama Minyak Atsiri dari Kulit Buah Jeruk Manis (Citrus sinensis L.) Asal Timor, Nusa Tenggara Timur Siburian, Rikson
Jurnal Natur Indonesia Vol 11, No 1 (2008)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Isolation and identifications of peel orange oils (Citrus sinensis, L.) which maserased by lard, coconut oil and mixof lard-coconut oil ( 2 : 1) has been done. This research aimed to obtain main component of peel orange oils fromTimor, knowing influence of lard, coconut oil and mix lard-coconut oils whom using as maserate to results ofchemistry components and atsiri’s oil rendemen product. In this research has been done by using maseration,extraction and using FT – IR and GC – MS as characterizations instruments. The results showed D- Limonen as amain component of atsiri oil from peel orange’s Timor.

Preparasi DNA Spesies Colletotrichum sp. dan Spesifitas Sistem Fingerprinting RAPD Jamsari, Jamsari
Jurnal Natur Indonesia Vol 11, No 1 (2008)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

DNA preparation from C. capsici dan C. gleosporides is important for PCR based analysis of anthracnose causingpathogens. On the other hand, RAPD as one of DNA-based fingerprinting is hindered by its instability and its nonspecifity problems. For that reason, converting RAPD fragments into other DNA based systems is an alternative toincrease its analysis reliability. The study showed that mycelia grown in 2-days liquid culture is appropriate materialfor DNA preparation especially when combined with Shagai-Maroof protocol as well as Promega Genomic DNAIsolation Kit. The study indicated also that some isolated RAPD-fragments showed its instability character. Thiswas proved by the occurrence of multi different length of fragments after re-amplification of some single RAPDspecific fragments.

Komunitas Parasitoid Lalat Pengorok Daun pada Pertanaman Sayuran Dataran Tinggi Rustam, Rusli; Rauf, Aunu; Maryana, Nina; Pudjianto, Pudjianto; Dadang, Dadang
Jurnal Natur Indonesia Vol 11, No 1 (2008)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Leafminer fly, Liriomyza spp. are widely known as new important pests attacking many vegetable species inhigh land in Indonesia. The objective of the research was to study community of parasitoid leafminer in highaltitude, and parasitization level. This research also looked at more specific on parasitization level caused byOpius chromatomyiae. There were four species of leafminer recorded from the survey, Liriomyza huidobrensis,Liriomyza sativae, Liriomyza chinensis and Chromatomyia horticola. The observation also found eight species ofparasitioid associated with collected leafminers. Six species of recorded parasitoid belonged to family of Eulopidae,and two rest species belonged to Braconidae and Eucoilidae respectively. Those eight species of parasitoids wereHemiptarsenus varicornis, Asecodes deluchii, Neochrysocharis okazakii, Neochrysocharis formosa ,Neocrhysocharis sp. Quadrastichus liriomyzae, Opius chromatomyaie, Gronotoma micromorpha. ParasitoidOpius chromatomyiae and Hemiptarsenus varicornis were found to be dominant in the range altitude between1001-1300 and 1301-1600 asl. From 16 spesies of hosts attacked by leaf miners, only Opius chromatomyiae wasrecorded to have ability to parasitize the leafminer in 13 host species with parasitization level reached 1,84–62,26%.

Micro Propagation of Several Potted Anthurium Accessions Using Spathe Explants Budiarto, Kurniawan
Jurnal Natur Indonesia Vol 11, No 1 (2008)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

The introduction of new varieties and production system of anthuriums has faced some problems due to the lowmultiplication rates in conventional vegetative and genotypic alteration problem in reproductive propagation. Sincethen, in vitro propagation technique became important to be investigated. The research was carried out fromSeptember 2006 to August 2007 at the Indonesian Ornamental Crops Research Institute. A complete factorialexperiment was designed to accomplish two chronological in vitro activities. The first step dealt with differentincubation sites, i.e. dark and light conditions for callus induction of three potted anthurium accessions, namelyclone no. SM. 001, cv. Alphine and cv. Bonito. The best callus obtained from the incubation treatment were then,transferred into defined media with different formulations to get free-callus plantlets in the second steps. Theresults showed that more progressive callus development from spathe explant was detected on the media ofmodified Nitsch and Nitsch + 1 mg/l 2,D + 1 mg/l Kin + 1 mg/l BA stored at dark after 60 days incubation. After 45days transferring the callus in the same media on light conditions, torpedo-shaped callus was detected. Completeplanlets were obtained after 75 days reculturing the buds in Nitsch and Nitsch medium containing 2,4 D withvarious BA concentrations. Clone SM. 001 and cv. Bonito showed higher number of visible shoot and rootdevelopments compared to cv. Alphine. In general, better shoot and root developments and higher planlets survivedafter acclimatization were observed on the BA concentrations of 2 and 3 mg/l.

Statistical Significance Test for Neural Network Classification Rezeki, Sri; Subanar, Subanar; Guritno, Suryo
Jurnal Natur Indonesia Vol 11, No 1 (2008)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Model selection in neural networks can be guided by statistical procedures, such as hypothesis tests, informationcriteria and cross validation. Taking a statistical perspective is especially important for nonparametric models likeneural networks, because the reason for applying them is the lack of knowledge about an adequate functionalform. Many researchers have developed model selection strategies for neural networks which are based onstatistical concepts. In this paper, we focused on the model evaluation by implementing statistical significancetest. We used Wald-test to evaluate the relevance of parameters in the networks for classification problem.Parameters with no significance influence on any of the network outputs have to be removed. In general, theresults show that Wald-test work properly to determine significance of each weight from the selected model. Anempirical study by using Iris data yields all parameters in the network are significance, except bias at the firstoutput neuron.

Extraction of Chemical Warfare Agents from Soils: Case Study on O-ethyl S-2-(diisopropylamino)ethyl methylphosphonothiolate (VX) Zuas, Oman
Jurnal Natur Indonesia Vol 11, No 1 (2008)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Extraction of chemical warfare agents, O-ethyl S-2-(diisopropylamino)ethyl methylphosphonothiolate (VX) in soilsample has been carried out. The extraction was performed using six different solvents including 1% TEA/MeOH,1% NH4OH/MeOH, water pH 2 at the ambient temperature , water pH 2 at temperature 4 0C, water pH 7 at ambienttemperature, and water pH 7 at temperature 4 0C. Percent recovery of VX in soil samples was quantitativelydetermined by mean LC-MS using selected reaction monitoring (SRM). Among the solvents used, water pH 2 attemperature 40C gave the best extraction capability that was indicated by the highest percent recovery of VXobtained. Storing effect of spiked samples was also investigated by extracting the samples containing VX usingwater at pH 2/40C and the degradation product was then identified using multiple reaction monitoring (MRM). Fromthe study, two degradation products were identified as Bis[2-(diisopropylamino)ethyl]disulphide and ethylmethylphosphonate.

Pemarasitan Benalu Dendrophthoe pentandra (L.) Miq. pada Tanaman Koleksi Kebun Raya Cibodas, Jawa Barat Sunaryo, Sunaryo
Jurnal Natur Indonesia Vol 11, No 1 (2008)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

The mistletoes of Dendrophthoe pentandra (Loranthaceae) grow as parasite on several plant collections of CibodasBotanical Gardens. Plant collections which attacked by mistletoe are Ficus type (Moraceae), and by individual innumber at most is Syzygium racemosum (Myrtaceae). 299 infected and uninfected branches of 67 host specieswere observed. The results showed that the host branches can be destroyed by mistletoe. Moreover, in the naturalconditions, the mistletoe causes degraded of the distal part of the infected branches.

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Irwan Effendi

Contact Email
natur@ejournal.unri.ac.id

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+62811764031

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kurniawanronal09@gmail.com

Editorial Address
Kampus Bina Widya KM. 12,5 Simpang Baru, Tampan, Pekanbaru 28293 Indonesia

Location
Kota pekanbaru,

Riau

INDONESIA

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Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Irwan Effendi

Contact Email natur@ejournal.unri.ac.id

Phone +62811764031

Journal Mail Official kurniawanronal09@gmail.com

Editorial Address Kampus Bina Widya KM. 12,5 Simpang Baru, Tampan, Pekanbaru 28293 Indonesia

Location Kota pekanbaru, Riau INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Irwan Effendi

Contact Email
natur@ejournal.unri.ac.id

Phone
+62811764031

Journal Mail Official
kurniawanronal09@gmail.com

Editorial Address
Kampus Bina Widya KM. 12,5 Simpang Baru, Tampan, Pekanbaru 28293 Indonesia

Location
Kota pekanbaru,

Riau

INDONESIA