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INDONESIA
Jurnal Natur Indonesia
Published by Universitas Riau
ISSN : 14109379     EISSN : 25030345     DOI : https://doi.org/10.31258/jnat
Core Subject : Science, Agriculture, Engineering,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry
Jurnal Natur Indonesia published since 1998, is a science journal that presents articles on research results, thoughts and views from researchers and experts in the field of biosciences (basic science), including biology, physics, chemistry and mathematics. In the field of biosciences (basic science); Biology, Physics, Chemistry, Mathematics.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 6 Documents
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Search results for , issue "Vol 15, No 2 (2013)" : 6 Documents clear
Purifikasi Parsial Enzim Ekstraseluler (Anoxybacillus sp.) yang Diisolasi dari Sumber Air Panas Bukit Kili Solok serta Aplikasinya untuk Menghidrolisis Limbah Berserat Octarya, Zona; Syukur, Sumaryati; Purwati, Endang
Jurnal Natur Indonesia Vol 15, No 2 (2013)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (233.856 KB) | DOI: 10.31258/jnat.15.2.106-114

Abstract

Termostable enzyme from thermophilic bacteria is very potential to improve technical enzyme in industry which used hightemperature. High water temperature exerts selection pressure on microbial species leading to specific flora that survivesand tolerates heat stress. The relative isolation and unique physical properties of Bukit Kili Ketek Hot Springs in Solok,West Sumatera may yield unique thermophiles. The generation of extracellular enzymatic bacterial is highly desirable forproduction of hydrolitic enzymes, which are useful in various industrial application and in animal feeds. This study,conducted to purify extracellular enzymes from thermophilic bacteria (Anoxybacillus sp.). This bacteria was isolated inBukit Kili Ketek Hot Springs, and after identified by analysis of 16S rRNA gene, 97% of similiarty with Anoxybacillus sp.was of obtained. The temperature of the hot waters was 52°C and the pH was 8. Extracellular and hydrolytic enzymeproduction were screened by qualitative SDS-PAGE method. SDS-PAGE analysis gave protein bands at ±110 kDa, ±80 kDa,±60 kDa, 50 kDa, 25 kDa, and ±10 kDa, respectively. Extracellular enzymes were used to degrade cellulose waste. Thecellulose activity for degradation of baggasse and pineapple pulp was 0,451 IU/mL and 0,310 IU/mL at 50°C and pH 6.
Analisis Asam Lemak Omega-3 dari Minyak Kepala Ikan Sunglir (Elagatis bipinnulata) melalui Esterifikasi Enzimatik Handayani, Sri Seno; Gunawan, Erin Ryantin; Kurniawati, Lely; Murniati, Murniati; Budiarto, Lalu Haris
Jurnal Natur Indonesia Vol 15, No 2 (2013)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (153.859 KB) | DOI: 10.31258/jnat.15.2.75-83

Abstract

Omega-3 fatty acid is the essential fatty acid and important for human health. Omega-3 fatty acid is also really neededby pregnant and lactating mothers and also children to prevent the malnutrition. Omega-3 fatty acid is also reallyneeded by pregnant and lactating mothers, and children to prevent the malnutrition. The omega-3 fatty acid is commonlyfound in some fish like salmon, tuna, hering, and mackarel. However, those fish are expensive, so alternativesources relatively cheaper fish. One of fish that is low in price and high in production in Nusa Tenggara Barat is Sunglir(Elagatis bipinnulata ). The purpose of this research was to determine free fatty acids content, type of omega-3 fattyacids, and their compositions in head fish oil. Head of sunglir fish was used as a sample because the head was usuallynot consumed. Sunglir fish was obtained from the Ampenan beach (NTB) with a size of 20–30 cm. The extraction methodused in this study was soxhletation with n-hexane solvent and analyzed with Gas Chromatografi (GC), acid ethyl estersfrom the esterification enzymatic of fish oil. Result revealed that the fish oil contained 84% free fatty acid and 0.85%linolenic acid (ALA), 2.80% eicosatrienoic acid (ETA), 0.73% eicosapentaenoic acid (EPA) and 2.41% docosahexaenoicacid (DHA). Saponification and iodine number of head fish oil is 248.24 mg KOH/g oil and 227.16 g Iod/100 g oil.
Populasi Mikrob di Rizosfer dan Pertumbuhan Caisim (Brassica juncea) di Tanah Dikontaminasi Insektisida Organoklorin setelah Aplikasi Konsorsia Mikrob dan Kompos Hindersah, Reginawanti; Rachman, Wahyuda; Fitriatin, Betty Natalie; Nursyamsi, Dedi
Jurnal Natur Indonesia Vol 15, No 2 (2013)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (79.076 KB) | DOI: 10.31258/jnat.15.2.115-120

Abstract

Organochlorine insecticide hed been banned for agriculture but nowadays its residue was still found in some agriculturalsoil. One of ways to lower organochlorine residue in soil is by using degrading-organochlorine microbial consortiaaccompanied by compost application. However, application of exogenous microbes might affect bacterial and fungalpopulation in soil and hence plant growth. The pot experiment has been set up to verify the influence of Pseudomonasmallei and Trichoderma sp. and compost on total bacteria and fungi on rhizosphere of caisim (Brassica juncea L.) grownon Andisols which were contaminated with organochlorine insecticide of Heptachlor, endosulfan, dieldrin, and DDT.Experimental design was Factorial Randomized Block Design with three replicates. The first factor was microbial consortiaof P.mallei and Trichoderma sp., and the second one was the kind of compost. The result showed that effect of microbialconsortia on total bacteria population was determined by the kind of compost. Total bacterial population in rhizosphere ofcaisim grown with cow manure compost and microbial consortia was more increased. However, microbial consortia as wellas any kind of compost did not influence total fungal population in caisim rhizosphere. Amount of leaf increased due tomicrobial and compost application however plant yield did not.
Sintesis Tiga Peptida Bergugus Pelindung sebagai Prekursor Komponen Vaksin Influenza Universal Subroto, Toto; Hardianto, Ari; Kahari, Abdul Alim; Pradnjaparamita, Tika
Jurnal Natur Indonesia Vol 15, No 2 (2013)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (533.702 KB) | DOI: 10.31258/jnat.15.2.84-91

Abstract

Current highly effective conventional vaccine to halt the spread of bird flu has not been invented yet because of susceptiblemutation of influenza virus. In spite of undergoing mutation which causes the amino acid sequence change, influenzaviruses maintain conservation at ectodomain of M2 protein, especially M2e(2-16) (SLLTEVETPIRNEW). The use ofconserved epitope M2e(2-16) in epitope-based vaccine potentially produces universal influenza vaccine. In designingepitope-based vaccine, the M2e(2-16) needs to be coupled with T helper epitope, P25, which is subsequently mentioned asM2e(2-16)-K-P25 (SLLTEVETPIRNEWGKKKL IPNASLIENCTKAEL). The M2e(2-16)-K-P25 was synthesized usingconvergent solid phase peptide synthesis strategy because of the size of the sequence. In this strategy, four peptideprecursors of M2e(2-16)-K-P25; SLLTEVETP (F1), IRNEWGK (F2), KLIPNASLI (F3), and ENCTKAEL (F4); were synthesizedin advance. After the precursors ready, coupling reaction was performed to obtain M2e(2-16)-K-P25. In the previousresearch, F3 has been obtained in high purity through Fmoc/tBu solid phase peptide synthesis method. In this conductedresearch, the three remaining precursors; F1, F2, and F4; were synthesized by the same method. Each peptide was analysedby thin layer chromatography, HPLC, and mass spectroscopy methods. F1, F2 and F4 were successfully synthesized andeach of them was detected at 1490.0, 1874.8 and 1881.9 amu, respectively. However, F1 was not possible to purify becauseof its insolubility in various solvents.
Hidrolisis Pati Ganyong (Canna edulis) dengan Amilase Bakteri Flavobacterium sp. PTBT I untuk Produksi Bioetanol Ningsih, Dian Riana; Zusfahair, Zusfahair; Fatoni, Amin
Jurnal Natur Indonesia Vol 15, No 2 (2013)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (205.385 KB) | DOI: 10.31258/jnat.15.2.92-98

Abstract

Bioethanol is an alternative energy of fuels produced from vegetable materials. Vegetable materials that can be used as rawmaterial for bioethanol is ganyong because it contains 22.60 g starch in 100 g ganyong. The production of bioethanol fromstarch material consisted of two steps, hydrolysis and fermentation. One of the steps to increase the value of bioethanolfrom starch of ganyong was hydrolysis process using thermostable amylase enzyme isolated from Flavoacterium sp.PTBT I bacteria was isolated from hot spring of Pancuran Tujuh Baturraden. The aim of this research was to use thermostableamylase to hydrolyze starch of ganyong and glucose produced to result bioethanol. The result of this research showed thatthe optimum condition hydrolysis starch of ganyong was using thermostable amylase acquired at substrate concentrationof 3% (b/v), and incubation time of about 75 minutes. The value of bioethanol increased with time of fermentation, from thefirst to fourth day, which was 0.8361; 2.2379; 5.7590 and 10.5787% (v/v), respectively.
Isolation and Characterization of Phytase from Chicken Manure Bacteria Irawan, Mega Pratiwi; Nurachman, Zeily
Jurnal Natur Indonesia Vol 15, No 2 (2013)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (339.527 KB) | DOI: 10.31258/jnat.15.2.99-105

Abstract

Cereals in animal feed contain anti-nutrients of phytic acid that has capability of chelating proteins and cations. Phytasecan be employed to reduce phytic acid through hydrolyzing phytic acid into free phosphate group and lower derivate ofinositol phosphate. The aim of the study was to isolate and characterize phytase obtained from chicken manure bacteria.The study included the screening of phytase-producing bacteria from chicken manure, the homology analysis of bacterium,and the determination of phytase activity. Phytase activity was measured from concentration of free phosphate. The resultsshowed that one phytase-producing isolate obtained from chicken manure grew in the medium containing 5% rice branextract at 37°C for 5 d. Based on a phylogeny is tree analysis of the genes related to 16S rRNA, the isolate was identified asAcinetobacter sp. TZ1. The extracellular phytase expressed by Acinetobacter sp. TZ1 exhibited optimum reactions at pH5 and 50°C. The enzyme showed activity of 64,6 nmol mL–1min–1 and specific activity of 236 nmol min–1mg–1. Relativemolecular mass of phytase TZ1 was ~35 kDa. Phytase obtained is potential to improve animal feed quality by hydrolyzingphytic acid.

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