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All Journal VALENSI JURNAL KIMIA SAINS DAN APLIKASI Jurnal Natur Indonesia The Journal of Pure and Applied Chemistry Research Jurnal Kimia Riset ALCHEMY Jurnal Penelitian Kimia Molekul: Jurnal Ilmiah Kimia Dinamika Journal : Pengabdian Masyarakat Jurnal Serambi Abdimas Jurnal Natural Darma Sabha Cendekia Molekul: Jurnal Ilmiah Kimia

Zusfahair Zusfahair

Jurusan Kimia Fakultas MIPA Universitas Jenderal Soedirman Purwokerto

Author-ID : 206720

Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Economics, Econometrics & Finance Energy Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Physics Other

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Isolasi, Pemurnian dan Karakterisasi Lipase Bakteri Hasil Skrining dari Tanah Tempat Pembuangan Akhir (TPA) Gunung Tugel Banyumas Zusfahair, Zusfahair; Setyaningtyas, Tien; Fatoni, Amin
Jurnal Natur Indonesia Vol 12, No 2 (2010)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

A bacterial lipase producer was isolated from garbage dump soil and was identified its genus. Lipase was extractedaccording to production time optimized, purified using ammonium sulfate fractionation and gel chromatograph.Determination of enzyme characteristic studied were influence of pH, temperature, various metals to lipaseactivity. The result of this research shows that the genus of isolated bacteria which produced lipase wasAcinetobacter sp., the lipase optimum production time is about 18 hours with the activity is about 115 unit/mL. Thehighest activity of lipase fractionation using ammonium sulfate is about 45% and the highest activity of purifyingwith filtration gel chromatograph column using Sephadex G-150 at 24 th fraction. Lipase from crude extract andpurifying product at this fraction has optimum pH 6 and optimum temperature is about 40 oC. Lipase to be classifiedas metalloenzyme that shows with decreasing the activity after added the EDTA. Metals ion, such as Cu 2+ and Zn2+were inhibited the lipase activity. Ca 2+ ion could increase lipase crude extract activity but inhibited the activity oflipase purifying product. Hg2+ ion could increase the activity of lipase purifying product.

Isolasi dan Karakterisasi Protease Ekstraseluler dari Bakteri dalam Limbah Cair Tahu Fatoni, Amin; Zusfahair, Zusfahair; Lestari, Puji
Jurnal Natur Indonesia Vol 10, No 2 (2008)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Protease has been used in large application industrial process such as detergent, leather, textil, softdrink, andmedicine. In order to find unique protease, many substances were explored as proteases of bacteria sources. Inthis study, tofu liquid waste was used as a source of bacteria producing proteases. Waste sample was growth inskim milk agar medium showing proteases activity, it was used to produce extracellular protease. The microbialcolonies were identified as Staphyllococcus sp. Protease was extracted with 5000 g centrifugation at 4 0C, andpurificated with ammonium sulphate precipitation continued with dialisis. Optimum production time, pH, metal ion,EDTA, specific activity, KM, and Vmaks were studied for enzyme characterization. Volume of crude enzyme was 300ml, with spesific activity of 3.55 U/mg. Protease obtained from 60% ammonium sulphate fraction had the highestspecific activity of 68.22 U/mg. Study on the protease characterization revealed that optimum temperature of thisenzyme was 400C. The optimum pH of the enzyme was found to be 8.0. The kinetic parameters K M dan Vmaks withcasein as substrate were 0.31% and 51.55 U/ml. Some inhibitory effect was observed in the presence of EDTA, Cu +2,Co+2, Zn+2, and enzyme activity was stimulated by Mg+2, indicating that this ion had a functional role in the molecularstructure of the enzyme.

Hidrolisis Pati Ganyong (Canna edulis) dengan Amilase Bakteri Flavobacterium sp. PTBT I untuk Produksi Bioetanol Ningsih, Dian Riana; Zusfahair, Zusfahair; Fatoni, Amin
Jurnal Natur Indonesia Vol 15, No 2 (2013)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Bioethanol is an alternative energy of fuels produced from vegetable materials. Vegetable materials that can be used as rawmaterial for bioethanol is ganyong because it contains 22.60 g starch in 100 g ganyong. The production of bioethanol fromstarch material consisted of two steps, hydrolysis and fermentation. One of the steps to increase the value of bioethanolfrom starch of ganyong was hydrolysis process using thermostable amylase enzyme isolated from Flavoacterium sp.PTBT I bacteria was isolated from hot spring of Pancuran Tujuh Baturraden. The aim of this research was to use thermostableamylase to hydrolyze starch of ganyong and glucose produced to result bioethanol. The result of this research showed thatthe optimum condition hydrolysis starch of ganyong was using thermostable amylase acquired at substrate concentrationof 3% (b/v), and incubation time of about 75 minutes. The value of bioethanol increased with time of fermentation, from thefirst to fourth day, which was 0.8361; 2.2379; 5.7590 and 10.5787% (v/v), respectively.

Determination of Cu and Pb concentrations based on urease activity inhibition of Durio zibethinus L. seeds Zusfahair, Zusfahair; Fatoni, Amin; Ningsih, Dian Riana; Riapanitra, Anung
Molekul Vol 16, No 2 (2021)
Publisher : Universitas Jenderal Soedirman

The determination of heavy metal concentrations has been carried out using sophisticated instruments, and therefore a simple and reliable alternative method is needed as a comparison. The study aimed to determine Cu and Pb concentration of standard solution using the urease activity inhibition method ofÂ Durio zibethinusÂ L.Â Â seeds.Â Â The research started with urease extraction fromÂ DurioÂ D.Â zibethinusÂ L.Â seeds. The activity of the obtained extract was determined using the Nessler method. The optimum substrate concentration was also determined. Urease activity inhibition was carried out using various metal solution concentrations, which continued by plotting a log graph of urea concentration vs. %inhibition. The obtained graph would then determine the metal concentration in a synthetic water sample.Â The data was then compared to the measurement, determined by the Atomic Absorption Spectrophotometry (AAS) method.Â Results of the study showed that the urease activity ofÂ D. zibethinusÂ L.seeds was 296.774 U/mL. Urease activity was optimum at a urea concentration of 0.3 M. The comparisonÂ Cu, and PbÂ concentration determination using the urease inhibitory activity and AAS methods showed no significant difference at 95% confidence level. This research showed that urease ofÂ D. zibethinusÂ L. seed could be used to determine Cu and Pb's concentration based on its inhibiting activity.

Aplikasi Urease dari Biji Kacang Tolo (Vigna unguiculata ssp unguiculata L.) untuk Biosensor Urea zusfahair zusfahair; Dian Riana Ningsih; Amin Fatoni; Vika Aprilia Puspitarini
Jurnal Kimia Valensi Jurnal Kimia VALENSI Volume 5, No. 1, May 2019
Publisher : Syarif Hidayatullah State Islamic University

Penggunaan urease dalam analisis urea yang digabungkan dengan suatu transduser disebut biosensor urea.Tujuan penelitian adalah menentukan kadar urea dengan metode biosensor urea berbasis urease biji kacang tolo yang diamobilisasi pada matrik alginat dan dideteksi secara kolorimetri menggunakan indikator bromtimol biru. Penelitian dimulai dengan isolasi urease dari biji kacang tolo (Vigna unguiculata ssp unguiculata L.), kemudian diamobilisasi menggunakan metode penjebakan dengan natrium alginat, setelah mencampur larutan urease dengan natrium alginat, diteteskan dalam larutan CaCl2 sampai terbentuk urease alginat. Beads urease alginat direaksikan dengan urea menghasilkan ion amonium, selanjutnya ditambahkan indikator bromtimol biru dan perubahan warnanya diukur menggunakan spektrofotometer. Kinerja analitis biosensor urea ditentukan melalui penentuan waktu reaksi enzimatis, keberulangan analisis, keberulangan pembuatan dan pengujian senyawa penganggu dengan konsentrasi urea 4mM, serta penentuan linearitas, batas deteksi, dan batas kuantifikasi dengan konsentrasi urea 0.05; 1; 3; 7; dan 15 mM. Hasil penelitian menunjukkan beads urease alginat bisa digunakan berulang sampai 8 kali. Kinerja analitis beads urease alginat menghasilkan respon yang linier pada rentang 0.05-15 mM dengan koefisien korelasi sebesar 0.9981, batas deteksi sebesar 0.8 mM dan batas kuantifikasi sebesar 2.67 mM. Keberulangan pembuatan beads urease alginat menghasilkan nilai koefisien variasi sebesar 6%. Analisis tidak terganggu dengan keberadaan asam askobat 0.05 mM dan asam urat 0.4 mM. Kata kunci: Amobilisasi urease, beads alginat, biosensor, biji kacang tolo, spektrofotometri. The use of urease in the urea analysis which combined with a transducer is called urea biosensor. Research aimed to determine urea level using urea biosensor method based on urease from black-eyed pea that immobilized on alginate matrix and detected by colorimetric using bromothymol blue indicator. The research began with urease isolation from black-eyed pea (Vigna unguiculata ssp unguiculata L.), and then it immobilized utilizing the trapping method with sodium alginate, after mixing urease solution with sodium alginate, it is dripped in CaCl2 solution until alginate urease beads formed. Alginate urease beads reacted with urea to produce ammonium ion, then it’s added with indicator bromothymol blue, and the color changes were measured using a spectrophotometer. The analytical performance of urea biosensor is determined by enzymatic reaction time, repeated analysis, repeatability of fabrication and calibration of disturbing compound with concentration of urea 4 mM, also linearity, limit of detection and limit of quantification with concentration of urea 0.05, 1, 3, 7 and 15 mM. The results showed that alginate urease beads could repeatedly be used until eight times. The analytical performance of alginate urease beads including a linear response in the range of 0.05 mM-15 mM with the correlation coefficient of 0.9981, the detection limit of 0.8 mM and the quantification limit of 2.67 mM. The repeatability of fabrication alginate urease beads produced the coefficient of variation value of 6%. The presence of 0.05 mM ascorbic acid and 0.4 mM uric acid.was not disrupted the analysis. Keywords: urease immobilization, alginate beads, biosensor, black-eyed pea, spectrophotometry.

Extraction and Characterization of Urease from Durio zibethinus L Zusfahair Zusfahair; Amin Fatoni; Dian Riana Ningsih; Mardiyah Kurniasih
Jurnal Kimia Valensi Jurnal Kimia VALENSI Volume 7, No. 2, November 2021
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v7i2.21768

Urease is a biocatalyst that serves to hydrolyze urea into ammonia and carbon dioxide. Since it is an imported product, the price of urea is still high. Urease can be found in grains. One of the grains that has not been explored for its urease content is durian (Durio zibethinus L.) seeds. This study aims to determine the effect of germination time on the activity of urease from durian seeds and its characteristics including the effect of pH, incubation temperature, enzymatic reaction time, addition of EDTA and metals, and storage time on the activity of urease from durian seeds. The first step of this study was seed germination which was carried out in the dark for 0, 3, 5, 7, and 9 days. Durian seed sprouts were extracted by mashing them using a mortar and pestle. They were then homogenized using a stirrer and centrifuged in a cold state. The crude urease extract obtained was then tested for its activity using the Nessler method. The acquired data was tested statistically using ANOVA. The results showed that the activity of urease from durian seeds was optimum at 3-day germination time, pH 7, incubation temperature at 30 °C, and 15-minute enzymatic reaction time with an activity of 163.6 U/mL. Urease is a metalloenzyme with its inhibitor being the Cu2+ and Na+ metal ions and its activator being Ba2+ metal ion. Tukey's test analysis showed that the effect of urease storage time at 4 °C resulted in a stable urease activity for 8 days while at room temperature it decreased its activity significantly to 72.8%.

Peningkatan Pendapatan Melalui Pembuatan Nata de Coco di Kelurahan Bancarkembar Purwokerto Utara Senny Widyaningsih; Zusfahair Zusfahair; Purwati Purwati
Dinamika Journal : Pengabdian Masyarakat Vol 1, No 4 (2019)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.dj.2019.1.4.895

Peningkatan pendapatan melalui pembuatan nata de coco telah dilakukan di Kelurahan Bancarkembar Kecamatan Purwokerto Utara Kabupaten Banyumas. Sasaran kegiatan ini adalah para pedagang jajanan sekolah. Para pedagang cenderung menggunakan bahan yang murah namun berbahaya sebagai bahan utama jualannya. Hal ini dikarenakan para penjual kebanyakan merupakan masyarakat yang berpenghasilan rendah. Upaya peningkatan pendapatan sangat diperlukan oleh mereka. Salah satu solusinya adalah dengan membina dan melatih mereka membuat makanan sehat yang dibuat dari bahan yang murah. Nata adalah salah satu makanan yang sehat yang dibuat dari bahan yang murah. Produk nata yang paling mudah pembuatannya adalah nata de coco yang dibuat dari air kelapa dengan metode fermentasi menggunakan bakteri Acetobacter xylinum. Kegiatan ini dilakukan melalui dua tahap. Tahap pertama adalah memberikan informasi melalui penyuluhan dan peragaan. Tahap kedua adalah mempraktekkan cara pembuatan nata de coco. Teknologi pembuatan nata ini dapat diterapkan pada para penjual makanan di lingkungan sekolah dasar di Kelurahan Bancarkembar Kabupaten Banyumas.

Formulation of M/A-Type Ointment Dosage From Ethanol Extract of White Plumeria Leaves (Plumeria alba l.) Against Candida albicans Dian Riana Ningsih; Zusfahair Zusfahair; Dwi Kartika; Mei Lianasari
The Journal of Pure and Applied Chemistry Research Vol 7, No 3 (2018): Edition September-December 2018
Publisher : Chemistry Department, The University of Brawijaya

Candida albicans is one of fungi that can cause various infections. This fungal infection is known as candidiasis and some people often suffer from candidiasis. The sweaty and moisty skin is a factor that allows the growth of fungi causing skin diseases. The plants that can be used as antifungal is white plumeria leaves (Plumeria alba L.). Ethanol extract of white plumeria leaves contains alkaloid and saponin active compounds, to ease its use and to get the maximum effect, then white plumeria leaves are formulated into ointment dosage form. This research is to know the antifungal activity of extract, to formulate ointment and to know the characteristic of the extract. The research begins with determination of minimum inhibitory concentration (MIC) of extract against C. albicans, then formulation of ointment dosage. The physical properties of the ointment is tested including homogeneity test, pH, dispersive power, protection power, adhesion and hedonic and antifungal activity. The method used in determining MIC and the activity test of dosage is agar-well diffusion method. The MIC value of extract was 5 ppm with a 1.22 mm inhibition zone. Extract has a form of semisolid, white colour, distinctive odor, homogeneous, protective, pH of 5.07-5.59, dispersive power of 5,09-5,78 cm, adhesion of 1.00-2.33 seconds and antifungal activity of ointment at day 0 for concentrations of 5, 10 and 15 ppm respectively are 2.93; 5.2 and 7.87 mm and at day 15 for concentrations of 5, 10 and 15 ppm respectively are 3.68; 4.87 and 5.82 m

PEMANFAATAN PROTEASE DARI KULIT NANAS (Ananas comosus, L) DALAM DEGUMMING BENANG SUTERA Zusfahair Zusfahair; Amin Fatoni; Dian Riana Ningsih
Jurnal Kimia Riset Vol. 1 No. 1 (2016): Juni
Publisher : Universitas Airlangga

AbstrakProtease dalam bidang industri tekstil dapat berperan pada proses degumming benang sutera. Salah satu protease yang dapat digunakan untuk degumming benang sutera dapat diisolasi dari kulit nanas. Penelitian ini bertujuan untuk mengetahui potensi protease dari kulit nanas dalam proses degumming benang sutera. Isolasi protease dari kulit nanas dilakukan dengan ekstraksi menggunakan buffer fosfat dilanjutkan dengan sentrifugasi untuk memisahkan debrisnya. Ekstrak protease dari kulit nanas selanjutnya digunakan dalam proses degumming benang sutera pada suhu dan waktu perendaman tertentu. Benang sutera hasil proses degumming diamati secara visual, menggunakan mikroskop cahaya dan mikroskop electron (SEM). Hasil penelitian menunjukkan bahwa proses degumming benang sutera yang optimal dilakukan pada suhu 50 °C, dan waktu inkubasi selama 4 jam. Benang sutera yang dihasilkan dengan protease ini lebih lembut dan berkilau, jika dibandingkan dengan benang sutera yang diolah secara tradisional menggunakan sabun dan pemanasan. Kata kunci: degumming, protease, kulit nanas, sutera AbstractProtease could be used in the textile industry for degumming of silk fabric. One of prospective proteases for silk degumming that of from a pineapple peels. This study was performed to determine the potential of protease from pineapple peels for silk degumming. The protease was extracted from pineapple peels using phosphate buffer, continued by separating the crude protease from the debris using centrifugation. The crude protease was then used to degum the raw silk, by soaking it in certain of incubation time and temperature. Silk degumming results observed visually, also by light microscope and electron microscope. The results showed that the optimum degumming process is at 50 ° C incubation for 4 hours. In comparison to the traditional silk degumming using soap and heating, the protease based degumming showed a softer and shiny silk fabric. Keyword: degumming, protease, pineapple peel, silk.

Pelatihan Kreasi Jajanan di Kelurahan Bancarkembar Kecamatan Purwokerto Utara Kabupaten Banyumas Mardiyah Kurniasih; zusfahair zusfahair; purwati purwati
Serambi Abdimas Vol 1 No 02 (2020): Jurnal Serambi Abdimas
Publisher : Universitas Jenderal Soedirman

Kegiatan pelatihan untuk anggota kelompok PKK (Pemberdayaan Kesejahteraan keluarga) adalah bentuk kepedulian kepada para perempuan dimana saat ini dituntut untuk selalu aktif kreatif dan kritis dalam memenuhi tuntutan zaman yang semakin global. Tujuan kegiatan ini adalah memotivasi anggota kelompok PKK khususnys di RT 03 RW 01 dan RT 03 RW 02 Kelurahan Bancarkembar Kecamatan purwokerto Utara Kabupaten Banyumas untuk membuat rumah industri yang dapat meningkatkan pendapatan keluarga. Kegiatan yang dilakukan adalah pelatihan pembuatan kreasi jajanan dan pendampingan rumah industri. Kegiatan ini telah terlaksana dengan lancar. Para peserta sangat antusius dengan kegiatan yang dilakukan oleh tim pengabdian. Melalui kegiatan ini terbentuk beberapa rumah industri di kelurahan Bancarkembar Kecamatan purwokerto Utara Kabupaten Banyumas. Rumah industri yang terbentuk antara lain rumah industri martabak mini, onde-onde dan kreasi coklat.

Co-Authors A.A. Ketut Agung Cahyawan W Ahmad Nurdin AMIN FATONI Anung Riapanitra Apriliadina, Inessa Putri Ari Asnani Aryanti, Eva Bilalodin Bilalodin Dadan Hermawan Darul Santri Pertiwi Dian Riana Ningsih Dian Riana Ningsih Dian Riana Ningsih Dian Riana Ningsih Dian Riana Ningsih Dian Riana Ningsih Dian Riana Ningsih Dian Riana Ningsih DIAN RIANA NINGSIH Dian Riana Ningsih Dian Riana Ningsih Dian Riana Ningsih Dian Riana Ningsih Dian Riana Ningsih Dian Riana Ningsih Dian Riana Ningsih Dian Riana Ningsih Dian Riana Ningsih Diyu Mantari Dwi Kartika Elok Dwi Putri Lestari ELY SETIAWAN Fajar Nuradha Febrina Nur Habibah Indah Permatawati Irmanto Irmanto Istinganatun Khoeriyah Luthfia, Adilla Mardiyah Kurniasih Mardiyah Kurniasih Mei Lianasari Mekar Dwi Anggraeni Muslihah, Niken Istikhari Niken Istikhari Muslihah Nuraini, Aprilia Nafi Puji Lestari PUJI LESTARI Puji Lestari Puji Lestari Purwati Purwati Purwati Purwati Purwati Purwati Purwati Purwati Purwati Purwati Senny Widyaningsih Senny Widyaningsih Senny Widyaningsih Setiawan, Ely Sulistyowati, Aris Suyata Suyata Tien Setyaningtyas Uyi Sulaeman Vika Aprilia Puspitarini

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