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INDONESIA
Life Science
Published by Universitas Negeri Semarang
ISSN : 22526277     EISSN : 25285009     DOI : https://doi.org/10.15294/lifesci
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Life Science publishes original and significant articles on all aspects of Life Sciences (Biology, Genetics, Biological Anthropology, Botany, Medical Sciences, Veterinary Sciences, Biochemical Genetics, Biometry, Clinical Genetics, Cytogenetics, Genetic Epidemiology, Genetic Testing, Evolution and Population Genetics, Immunogenetics and Molecular Genetics). The journal also covers ethical issues. It aims to serve as a forum for life scientists and health professionals.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 9 Documents
 1 
Search results for , issue "Vol 2 No 1 (2013)" : 9 Documents clear
PENGARUH VITAMIN E TERHADAP KADAR SGPT DAN SGOT TIKUS PUTIH GALUR WISTAR YANG DIPAPAR TIMBAL
Life Science Vol 2 No 1 (2013)
Publisher : Universitas Negeri Semarang

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Abstract

This study aimed to examine the effect of the vitamin E administration on levels of SGOT and SGPT blood serum male albino rats (Rattus norvegicus) wistar strain were exposed of lead per oral. The sample used 20 male rats were divided into 4 groups:I of a control, II of the treatment of lead 175 mg/rat, III of Vitamin E 1.44 mg/rat and lead 175 mg/rat, IV of Vitamin E 2.16 mg/rat and lead 175 mg/rat for 21 days. On day 22 measured levels of SGOT and SGPT and blood lead. SGOT and SGPT data were analyzed with one-way ANOVA, followed by BNT test. The results of one-way ANOVA showed antioxidant vitamin E giving a significant effect on the levels of SGOT and SGPT (p <0.05) rats. The BNT test SGOT levels showed a significant difference except in group II and III, the SGPT levels showed a significant difference except in group III and IV. From the results of this study concluded that the affects of vitamin E decrase levels of SGOT and SGPT blood serum of male albino rats (Rattus norvegicus) wistar strain were exposed to lead per oral.
DISTRIBUSI LONGITUDINAL BERBAGAI SPESIES IKAN DI SUNGAI DAMAR KABUPATEN KENDAL
Life Science Vol 2 No 1 (2013)
Publisher : Universitas Negeri Semarang

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Abstract

Damar River receives waste inputs from household industrial activity and agricultural activity that can effect on the distribution and the diversity of living things in it, including the fish. This research is exploration research, with the sampling was conducted at 5 stations in different locations. The samples were collected at 3 different sampling times with 2-week interval. Data was analysed using longitudinal distribution, based on the density of species per-station and per-period of sampling. The species diversity was also calculated using Shanon Diversity Index. There were 10 fish species that were distributed in the Damar River i.e. Anabas testudineus, Trichogaster trichopterus, Clarias batracus, Oreochormis niloticus, Arius caelatus, Periophtalmus malacensis, Scatophagus argus, Crenimugil heterocheilos, Pomadasys argenteus, and Sillago robusta. Species which was distributed along the Damar River was Anabas testudineus with the distribution center at river estuary. The Diversity Index value of Damar River is categorized as intermediate (1.88), thus it shows that the water quality criteria of Damar River is lightly polluted water.
KEANEKARAGAMAN FITOPLANKTON DI ALIRAN SUMBER AIR PANAS CONDRODIMUKO GEDONGSONGO KABUPATEN SEMARANG
Life Science Vol 2 No 1 (2013)
Publisher : Universitas Negeri Semarang

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Abstract

Condrodimuko Gedongsongo is one of the hot water sources where placed at north of Ungaran mountain in Kabupaten Semarang. The hot temperature and mineral content of the hot waters only tolerant phytoplankton that can be survive in this condition. The aim of this research was to know the species diversity of phytoplankton in the flow of hot waters in Condrodimuko Gedongsongo this condition. Selected sampling purpossive sampling at three stations, each station do three sampling period. The species number and the individual number of the phytoplankton were taken as data to conclude the diversity species of phytoplankton using Shannon-Wiener method. The result of the research was nine species of phytoplankton were found there are Cladopora sp, Gonatozygon sp, Microspora sp, Oscillatoria sp, Phormidium sp, Protococcus sp, Tribonema sp, Diatom sp dan Ophiocytium sp. The result of analysis on phytoplankton diversity of hot water sources at the Condrodimuko Gedongsongo Kabupaten Semarang the value 2,09 and is one of high diversity.
UJI RESISTENSI Mycobacterium tuberculosis TERHADAP OBAT ANTI TUBERKULOSIS (OAT) DENGAN METODE PENIPISAN
Life Science Vol 2 No 1 (2013)
Publisher : Universitas Negeri Semarang

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Abstract

The purpose of this research is to find out the reaction of M. tuberculosis resistance to OAT whit dilution method. This research is exploration research with post randomized controlled design. Each sample made as suspension with Mc Farland 1 turbidity then experimented with PNB to detect Mycobacterium Other Than Tuberculosis (MOTT). Negative result of PNB experiment then resistance experimented. Resistance experiment grown in Lowenstein-Jeensen (L-J) medium as control and were mixed is L-J medium which had been added with each OAT that is Isoniazid 0,2 mg/L, Rifampisin 40 mg/L, Streptomisin 4 mg/L dan Etambutanol 2 mg/L. Experiment result of PNB shows that there are five negative sample and one MOTT positive sample. Resistance experiment with dilution shows that there are 5 sensitive samples to OAT. From the research it can be concluded that by dilution method is not really influencing M. tuberculosis resistance experiment to OAT.
SAPROBITAS PERAIRAN SUNGAI JUWANA BERDASARKAN BIOINDIKATOR PLANKTON
Life Science Vol 2 No 1 (2013)
Publisher : Universitas Negeri Semarang

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Sungai Juwana merupakan sungai terbesar dan terpanjang di Kota Pati. Berkembangnya kegiatan penduduk di Daerah Aliran Sungai (DAS) Juwana dapat mempengaruhi kualitas air sungai. Penelitian ini menggunakan rancangan eksplorasi dengan metode survai, dan penetapan stasiun pengambilan sampel dengan metode purposive sampling. Penempatan stasiun didasarkan atas perkiraan beban pencemar yang masuk ke sungai. Stasiun 1 berada hulu sebagai pembanding, stasiun 2 sumber limbah pabrik tahu dan pertanian, stasiun 3 sumber limbah industri kacang, stasiun 4 sumber limbah industri peleburan timah, stasiun 5 limbah ikan dari TPI serta pelabuhan, dan stasiun 6 berada di hilir sungai (dekat muara). Pengambilan sampel dilakukan sebanyak 3 kali dengan selang waktu 2 minggu. Hasil penelitian ditemukan 10 jenis plankton terdiri dari 3 jenis kelompok α-Mesosaprobik, 2 jenis kelompok β-Mesosaprobrik dan 5 jenis kelompok non saprobik, kemudian diinterpretasikan pada tabel hubungan antara koefisien saprobitas perairan dengan tingkat pencemaran perairan. Berdasarkan perhitungan dan analisis nilai koefisien saprobik dari stasiun 1 sampai hilir didapatkan nilai koefisien saprobik plankton berkisar antara -0,6 s/d -1. Berdasarkan kriteria tingkat pencemaran menunjukkan bahwa Sungai Juwana berada dalam kondisi tercemar sedang sampai dengan cukup berat.Juwana River is the largest and longest river in the city of Pati. The developing activities in the watershed Juwana can affect water quality. Exploratory study used a survey method, where the determination of sampling stations using purposive sampling. The settlement of the stations was conducted based on the estimated pollutant loads into the river, the first station was located in the upstream of the river, as comparison the second station was located nearby peanut industry, next the fourth station was located in the tinmelting industrial wastes, the fifth station was located in the fish market and nearby the Harbour, the last , sixth station was located in the downstream of the river. Sampling performed 3 times with an interval of 2 weeks. In this research, it has found that is 10 species of plankton consist of 3 species group α-Mesosaprobic, 2 species group β-Mesosaprobrik and 5 species group non saprobik, and than interpreted the relationship between the coefficient table saprobitas waters with levels of water pollution. Based on the calculation and analysis of the saprobic coefficient values station 1 to downstream obtainet plankton saprobic coefficient values ranged from -0.6 s / d -1. Based on these criteria indicate that the level of pollution in Juwana River is moderate to high.
PERKEMBANGAN OVARIUM BURUNG PUYUH YANG DIBERI VARIASI PENCAHAYAAN WARNA LAMPU SELAMA 16 JAM
Life Science Vol 2 No 1 (2013)
Publisher : Universitas Negeri Semarang

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Burung puyuh merupakan salah satu unggas yang sedang dikembangkan dan ditingkatkan produksinya. Untuk meningkatkan produksinya, berbagai pemeliharaan telah dilakukan. Salah satunya dengan memberikan program pencahayaan. Tujuan penelitian ini adalah untuk mengetahui perkembangan ovarium burung puyuh yang diberi variasi warna cahaya lampu dan warna cahaya yang paling optimal terhadap perkembangan ovarium. Burung puyuh (Coturnix-coturnix japonica) betina sebanyak 25 ekor berumur lima hari diaklimasi selama satu minggu. Selanjutnya dikelompokan menjadi lima perlakuan, masing-masing kelompok terdiri atas lima ekor puyuh yaitu kelompok kontrol menggunakan cahaya matahari, kelompok I cahaya lampu bening, kelompok II merah, kelompok III biru, dan kelompok IV kuning. Perlakuan cahaya diberikan selama 16 jam per hari (06.00-22.00 WIB) selama 30 hari. Selanjutnya burung puyuh dibedah dan diamati perkembangan ovarium yang meliputi berat badan burung puyuh, berat ovarium dan struktur makroskopis ovarium. Pemberian variasi warna lampu pencahayaan selama 30 hari dengan 16 jam ternag/hari berpengaruh terhadap perkembangan ovarium burung puyuh dan pencahayaan warna lampu merah merupakan pencahayaan yang paling optimal. Quail is one of poultry that are being developed and enhanced its production. To increase production, a variety of maintenance has been carried out. One of them is by giving the program the lighting. The purpose of this research is to know the development of quail ovarian given color variations of light and color the most optimal light towards the development of ovaries. Quail (Coturnix-coturnix japonica) females as much as 25 five-day-old tail diaklimasi for one week. Next up are grouped into five treatment groups, each consisting of five quail tail which is the control group using sun light, the light group I group II, red, clear, blue, group III and Group IV yellow. Light treatment is given for 16 hours per day (6 am-10 pm) for 30 days. Quail Furthermore dissected and observed the development of the ovary which include weight, the weight of the quail ovary and ovarian makroskopis structure. Provision of lighting lamp color variation for 30 days with 12 hours of light/day affect the ovarian development of quail and lighting color red lights is the most optimal lighting.
POPULASI JULANG EMAS (Aceros Undulatus) DI GUNUNG UNGARAN JAWA TENGAH
Life Science Vol 2 No 1 (2013)
Publisher : Universitas Negeri Semarang

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Penelitian ini bertujuan mengetahui Populasi Julang Emas di Gunung Ungaran. Data populasi diperoleh dengan menggunakan Metode Variable Circular Plot (VCP) dengan jarak radius pengamatan 1 Km (Luas VCP 0,535 Km²). Penelitian dilakukan pada bulan Juli- Agustus 2011 dan Januari 2012. Pengambilan data dimulai pukul 06.00- 16.00 WIB. Hasil penelitian menunjukanpopulasi Julang Emas di Gunung Ungaran adalah 15 ekor/Km². Populasi Julang Emas di Gunung Ungaran tersebar merata di empat stasiun dengan kepadatan populasi distasiun Medini (19,21 ekor/Km²), stasiun Banyuwindu (15,66 ekor/Km²), stasiun Gajah Mungkur (12,48 ekor/Km²) dan stasiun Gunung Gentong (10,5 ekor/Km²). Perbandingan rasio kelamin antara jantan dan betina yaitu 1:1 menunjukkan populasi Julang Emas di Gunung Ungaran berada pada musim tidak berbiak.The objective of this study was to determine the Wreathed Hornbill population at Mount Ungaran. Population data obtained using VCP (Variable Circular Plot) with 1 km diameter of circular plot (0.535 Km²). The study was conducted from July-Agustus 2011 and January 2012. The Observation started at 06:00 am to 04:00 pm. The results showed that the population of Wreathed Hornbill in Mount Ungaran was 15 individual/Km². The population of Wreathed Hornbill distributed was four station that were Medini station (19 individual/Km²), Banyuwindu station (15 individual/Km²), Gajah Mungkur station (12 individual/Km²) and Gunung Gentong station (10 individual/Km²). Sex ratio between male and female was 1:1, that showed the Wreathed Hornbill was not in breeding season.
MIKRO ANOTOMI INSANG IKAN SEBAGAI INDIKATOR PENCEMARAN LOGAM BERAT DI PERAIRAN KALIGARANG SEMARANG
Life Science Vol 2 No 1 (2013)
Publisher : Universitas Negeri Semarang

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PerairanKaligarangmerupakanperairanyangsangatpentingbagikehidupanpendudukdiKotaSemaranSelain masyarakat, ndutri-industri di sepanjang perairan tersebut juga menggunakan air PerairanKaligarang untuk keperluan proses produksi, sekaligus sebagai tempat pembuangan limbah padaakhir proses produksinya.Ikan sebagai salah satu biota air sering dijadikan sebagai salah satuindikator tingkat pencemaran yang terjadi di dalam perairan.Penelitian ini menggunakan rancanganeksplorasi karena melihat dari tingkatan pencemaran logam berat dengan menggunakan ikansebagai indikator. Pengambilan sampel diambil setiap 2-3 minggu sekali dalam jangka waktu selama1 bulan pada bulan November-Desember 2012. Data dalam penelitian ini adalah data kualitatifberupa tingkat kerusakan mikroantomi insang ikan yang terindikasi logam berat kemudiandihubungkan dengan faktor lingkungan meliputi Suhu, pH, BOD, COD.Berdasarkan pengamatanmikroanatomi insang ikan yang terindikasi logam berat terjadi kerusakan mikroanatomi sampai ketingkat nekrosis. Berdasarkan kriteria tingkat pencemaran menunjukkan bahwa Perairan Kaligarangberada dalam kondisi tercemar sedang sampai dengan cukup berat.Kaligarang river is the river which is very important to the life of residents in the city of Semarang. In additionto the community, industry was-industry along the river is also used Kaligarang River water for the productionprocess, as well as the disposal of waste at the end of the production process. Fish as one of the aquatic biota isoften used as an indicator of the level of contamination that occurred in the waters. This study used exploratorydesign as seen from the level of heavy metal pollution using fish as indicators. Sampling was taken every 2-3weeks for a period of one month in November-December 2012. The data in this study was qualitative data in theform of the damage fish gills mikroantomi indicated heavy metals were then linked to environmental factorsinclude temperature, pH, BOD, COD.Based on observations of fish gills mikroanatomi indicate mikroanatomidamage to the level of necrosis. Based on these criteria indicated that the level of pollution in a state Kaligarangriver polluted with moderate to heavy.
INDUKSI PERBANYAKAN TUNAS Rosa damascena Mill. DENGAN PENAMBAHAN AUKSIN DAN SITOKININ
Life Science Vol 2 No 1 (2013)
Publisher : Universitas Negeri Semarang

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Penelitian ini bertujuan untuk mengetahui optimasi auksin dan sitokinin pada induksi perbanyakan tunas mawar damaskus. Penelitian perbanyakan tunas menggunakan rancangan acak lengkap faktorial terdiri dari dua faktor yaitu variasi konsentrasi BA (1, 2, 3, dan 4 ppm) dan konsentrasi NAA (0,1; 0,3; 0,5 dan 0,7 ppm). Tunas lateral diinduksi pada media Murashige & Skoog dengan penambahan kombinasi BA dan NAA (16 kombinasi) selama enam minggu, yang diukur berdasarkan dua parameter yaitu rentang waktu muncul tunas pertama dan jumlah tunas. Data dianalisis menggunakan anava dua jalan dan uji Duncan. Hasil penelitian menunjukkan BA, NAA dan kombinasinya berpengaruh terhadap rentang waktu muncul tunas pertama, tetapi hanya BA yang berpengaruh terhadap jumlah tunas. Hasil analisis data menunjukkan bahwa kombinasi BA 3 ppm+NAA 0,3 ppm optimal untuk induksi rentang waktu muncul tunas pertama yakni 4 hari MST, sedangkan jumlah tunas terbanyak dihasilkan oleh BA 1 ppm dengan 7 tunas.This research aimed to know the optimation of auxin and cytokinin on damask rose multiple shoot induction. Shoot multiplication did by fledged random design factorial consist of two factors those were various BA concentration (1, 2, 3, and 4 ppm) and NAA concentration (0.1; 0.3; 0.5 and 0.7 ppm). Axillary shoots were induced on Murashige & Skoog medium supplemented with various BA and NAA combinations (16 combinations) for six weeks, measured based on two parameters, shoot initial timing and shoot numbers. Data was analized by two way anova and DMRT. The result showed that BA, NAA and it’s combination were significantly influenced on shoot initial timing induction, but only BA was significantly influenced on shoot numbers. Based on data analysis, 3 ppm+NAA 0,3 ppm BA combination was optimal for shoot initial timing induction in 4 days after plant and 1 ppm BA gave the highest shoot numbers with 7 shoots per nodal.

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