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Media Veteriner
Published by Institut Pertanian Bogor
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Articles 3 Documents
 1 
Search results for , issue "Vol. 5 No. 3 (1998): Media Veteriner" : 3 Documents clear
Ultrasonography I: Development of Ovarian Follicles in Boer Goats Superovulated With pFSH Containing 40 % pLH Suyadi Suyadi
Media Veteriner Vol. 5 No. 3 (1998): Media Veteriner
Publisher : Media Veteriner

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Abstract

Response of oestrus in Boer goats and development of ovarian follicle to superovulatory treatment were studied. All goats (n= 17) received subcutaneous progesterone ear implant for 11 days to synchronize oestrus cycle. Forty eight hours before implant removal and the following 3 days. 16 AU pFSH containing 40 % pLH was injected 6 times (4, 4; 2, 2; 2, 2) in 12 h intervals. Goats received PGF2a twice synchronized with FSH treatments to induce luteolysis (5 mg PGF2a of each) 24 h after implant removal. Animals were checked for oestrus with an aproned buck 3 times a day. During standing oestrus animals were naturally mated 2 or 3 times. Follicular development and ovulation were monitored 2 times a day using transrectal ultrasonography equipped with a 7.5 MHz linear-array transducer. This evaluation began from the time of implant removal until 48 h after the end of oestrus. Eighty eight per cent of animal showed oestrus with mean of the onset of oestrus 33 h after implant removal and oestrus duration of 30 h. Eighty two percent of does had corpora lutea with number of 11,0 and persistent follicles of 2.1 at laparoscopic examination on day 6 after the end of oestrus. The maximum number of follicles with diameter ≥ 2 mm recorded at the observation of 36 and 48 h after implant removal, whereas those with ≥ 6 mm in diameter were 48 - 60 h. The number of follicles with diameter 2 - 3 mm and 4 - 5 mm during observation period were constant. The ovulation of follicles did not occur spontaneously but they ovulated in several periods. The pFSH+ 40 % pLH preparation yielded a sufficient result for superovulation means in Boer goats. For monitoring the response of ovaries to the superovulatory treatment, ultrasonography was a simple and effective method.
Semen Production in Sambar Stag and Response to Oestrus Synchronization in Sambar Hind Gono Semiadi; Paul David Muir; Thomas Neville Barry; Geoff Asher
Media Veteriner Vol. 5 No. 3 (1998): Media Veteriner
Publisher : Media Veteriner

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Abstract

Studies on the production of spermatozoa and oestrus synchronization in sambar deer (Cervus unicolor) were conducted using nine stags and 12 hinds. Prior to the semen collection via electroejaculator stimulation, stags were sedated using a combination of fentanyl citrate, azaperone and xylazine hydrochloride (Fentazin®, Parnell Lab. NZ). Collected semen were evaluated for its volume, motility and concentration. Collected semen were evaluated for its volume, motility and concentration, placed into straw and then kept in frozen, chilled and fresh container. In hinds, the synchronization was conducted using intravaginal Controlled Internal Drug Release type G (CIDR-G®, 9 % w/w, 300 mg progesterone; Agricultural Division, CHH Products Group Ltd, Hamilton, NZ), followed by i.m injection of 250 IU PMSG. The results showed that stags and hind did not responp very well to all treatments. Mean of ejaculated semen volume was low (0.91 ml) and some ejaculated semen had 80 % motility. Any semen collection should be conducted based on the individual performance, with the age time in hard antler condition as the main factors. Half of the hinds responded to the synchronization 59.5 hrs after the injection of PMSG.
Maturing Singly Versus in Groups on Ovine Oocyte Meiosis Endang Tri Margawati
Media Veteriner Vol. 5 No. 3 (1998): Media Veteriner
Publisher : Media Veteriner

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Abstract

The effect of maturing systems (single vs groups) of ovine oocytes was studied to examine the oocyte development in vitro. Oocytes were aspirated from follicle ovaries collected at local abattoirs Cibinong West Java, using a 18-G needle. Aspiration medium consisted of H 199 + 2 % FCS + 50 μg/ml Heparin and the maturating medium (IVM) consisted of B 199 - 10 % FCS + 10 μg/ml FSH + 10 μg/ml hCG + 1 μg/ml Estradiol. The oocytes collected were divided into three groups and treated separately as follows: T1) oocytes were matured singly in 50 μl IVM medium. T2) every five oocytes was matured in 50 μl IVM medium and T3) every 10 oocytes was matured in 50 μl IVM medium. All oocytes were maintained in incubator with 5 % CO, and high humidity at 38 °C for 20 h. The resulting ova were stained in 1 % lacmoid then examined for meiosis division under a microscope. There was a signiticant effect among treatments on the proportion oocytes reaching metaphase II (P

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