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INDONESIA
Media Veteriner
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Articles 4 Documents
Search results for , issue "Vol. 5 No. 4 (1998): Media Veteriner" : 4 Documents clear
The Effect of Sublethal Concentration of Phosphamidon on Tissue Damage of Nile Fish (Oreochromis niloticliS Trew.) T Efrizal; Heru Setijanto; Djamar Tumpal F. Lumbanbatu; Yuhara Sukra
Media Veteriner Vol. 5 No. 4 (1998): Media Veteriner
Publisher : Media Veteriner

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Abstract

The effect of sublethal concentrations of phosphamidon on tissue damage of nile fish (Oreochromis niloticus Trew.) were studied at the Laboratory of Aquatic Animal Physiology Faculty of Fisheries and at the Laboratory of Anatomy F aculty of Veterinary Medicine Institut Pertanian Bogor. Renewal test method with 40 % of the media renewed everyday was used for the examination of the sublethal toxicity. Each test consisted of 30 nile fish in 15 1 water. The concentrations of phosphamidon used in this study was 0 mg/l (control group), 3.7 mg/l, 7.4 mg/l, dan 11.0 mg/l, respectively, and all groups were repeated three times. Histopathologically, the gill yielded secondary lamella damages, necrosis, hipertrophy, fusion lamella and degeneration of cartilaginous supportive tissue. The intestine showed necrosis of mucous cells, hipertrophy, bleeding in the lamina propria, and proliferation of connective tissue. In the hepatopancreas organ there were bleeding, infiltration of leucocytes and proliferation of connective tissue.
Native Virus Challenge Test Against Vaccinated Chickens With Commercial Active and Inactive IBD Vaccines Retno Damajanti Soejoedono
Media Veteriner Vol. 5 No. 4 (1998): Media Veteriner
Publisher : Media Veteriner

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Abstract

Vaccination trial were conducted on two groups of broiler day-old-chick (DOC) using active and inactive IBD vaccines. First Group was further divided into two groups: one received active IBD vaccination at 7 days of age, and the other received oral vaccination at 7 and 21 days of age, respectively. Both subgroups were then challenged against native IBD isolate, K-5, at 21 days of age. The second Group was arranged similar to the first group, but the second Group received inactive IBD vaccination subcutaneously, at 21 days of age. At 35 days of age, all chickens were challenged against native IBD isolate, K-5. The group which received active vaccine showed pathological change of the bursa, correlated to the bursa/body weight indices less than 0.70 (20 %) and the bursa lesion score (BLS) was 1.56. This pathological change was more obvious after second application of the vaccine. The group which received active and inactive vaccines revealed immune responses with mild lesion in the bursa. This im-munity could not protect the chickens after challenged with K-5 isolate, correlated to the bursa/body weight indices less than 0.70 (100 %) and BLS was 4.0. This results show that K-5 isolate belong to different subtype or variant.
Infection of Transmissible Gastroenteritis Virus in Pig Indrawati Sendow
Media Veteriner Vol. 5 No. 4 (1998): Media Veteriner
Publisher : Media Veteriner

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Abstract

TransmissIble gastroenteritis (TGE) virus belong to corona virus which cause acute diarrhoea in under two years old young pig, especially young piglets. The pigs experience severe loss of body fluid which can lead to death. To prevent the disease, vaccination is a must, but the vaccine available at present is not effective. It is quite difficult to diagnose TGE clinically and diarrhoea could be due to other agents, i.e. bacterial, parasite and another viral infections. A comprehensive conclusion of laboratory examination, pathological finding, and clinical signs in the field can lead to definitive diagnose of TGE. A review about the disease, causative agent, characteristic of the agent, clinical signs, epidemiology, diagnose, control and prevention, and the occurrence in Indonesia based on serological result is presented.
Relation Between Encapsulation of Streptococci of Serological Group B and Adherence Properties of The Bacteria to Deae-Sephacel Christoph Lammler; I Wayan Teguh Wibawan; Fachriyan H Pasaribu
Media Veteriner Vol. 5 No. 4 (1998): Media Veteriner
Publisher : Media Veteriner

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Abstract

Determination of surface charge of group B streptococci by ion exchange chromatography on DEAE-Sephacel revealed that bovine and human group B streptococcal isolates with protein surrface antigens alone, or bacteria with protein antigen in combination with polysacharide antigens, adhered strongly to the gel matrix. In contrary, cultures with polysacchaide antigens alone showed no comparable adherence properties. Removal of neuraminic acid from bacterial surface enhanced, but pronase treatment reduced the adherence values. The importance of type specific capsular sialylation for group B streptococcal surface charge could be confirmed with group B streptococci of serotype III and their transposon mutagenized asialocapsular mutants. In contrary to the encapsulated parent strains the asialo capsular mutants adhered strongly to the gel matrix. Comparable differences were observed with unencapsulated group B streptococcal variant strains and its isogenic encapsulated parent strains. The capsule material seemed to mask the surface proteins responsible for the adherence to the gel matrix. The determination of surface charge of group B streptococci by ion exchange chromatography might help to understand the importance of capsular sialylation for individual isolates of this bacterial species.

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