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Ivandini Tribidasari A.
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Makara Journal of Science
Published by Universitas Indonesia
ISSN : 23391995     EISSN : 23560851     DOI : https://doi.org/10.7454/mss
Core Subject :
Makara Journal of Science publishes original research or theoretical papers, notes, and minireviews on new knowledge and research or research applications on current issues in basic sciences, namely: Material Sciences (including: physics, biology, and chemistry); Biochemistry, Genetics, and Molecular Biology (including: microbiology, physiology, ecology, taxonomy and evolution); and Biotechnology.
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Articles 23 Documents
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Search results for , issue "Vol. 11, No. 2" : 23 Documents clear
PEMURNIAN DAN KARAKTERISASI XILANASE Streptomyces sp. SKK1-8 Meryandini, Anja; Widhyastuti, Nunuk; Lestari, Yulin
Makara Journal of Science Vol. 11, No. 2
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PENGARUH VARIASI INISIATOR DAN TEKNIK POLIMERISASI TERHADAP UKURAN PARTIKEL PADA KOPOLIMERISASI EMULSI STIRENA-BUTIL AKRILAT-METIL METAKRILAT Budianto, Emil; Ariyanti, Ariyanti
Makara Journal of Science Vol. 11, No. 2
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SEBARAN DAN LUAS HUTAN MANGROVE DI WILAYAH PESISIR TELUK PISING UTARA PULAU KABAENA PROVINSI SULAWESI TENGGARA Tarigan, M. Salam
Makara Journal of Science Vol. 11, No. 2
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Distribution and area of mangrove forest in Pising Bay coastal area North Kabaena Island Southeast Sulawesi. Researched of mangrove forest in Pising Bay, North Kabaena Island Southeast Sulawesi was carried out on April 2006. Data digital Landsat 7ETM+ parth/raw 113/064 was used for mangrove mapping. Classification method was done is multispectral supervised with artificial neural networks algortms. Analysis and data validation from field survey, it is estimated that distribution and areas of mangrove forest in Pising Bay, North Kabaena Island Southeast Sulawesi is 152.128 Ha. Mangrove in Pising Bay dominated by rhizophora apiculata, rhizophora mucronata; and the forest is relatively in good condition.
IDENTIFICATION OF SOME STRAINS OF DINOFLAGELLATES BASED ON MORPHOLOGY AND MOLECULAR ANALYSIS Thoha, Hikmah
Makara Journal of Science Vol. 11, No. 2
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Dinoflagellates are the important primary producers in aquatic environments. In oceans, they play interesting role in ecological functions such as red tide forming organisms, symbiont of coral reef or sea anemone and DSP (Diarrhetic Shellfish Poisoning) or PSP (Paralytic Shellfish Poisoning) producing organisms. Morphology and molecular analysis of dinoflagellates were conducted on November 2002 to March 2003. The phylogenetic studies based on 18S rDNA analyses, sequence have begun to appear more frequently in the literature, as attention has turned to relationships within the major eukaryotic lineages, particular importance for the taxonomy of the armored and unarmored genera of dinoflagellates (Gyrodinium sp., Cachonina sp., Gymnodinium sp., Amphidinium sp.), because many of the genera cause extensive plankton blooms, fish kills and other harmful events, were studied used to amplify 18S rDNA, present in the total DNA extracted from algal pellet. The amplify approximately 1400 bp of the nuclear-encoded LSU rDNA gene using terminal primeirs DIR, products were cheked by 1.0 % agarose gel electrophoresis, then cloning with TA cloning KIT. Sequencing were analyzed by the GENETIX Mac Software, Homology search by Blast and Phylogenetic analysis. Results of hylogenetic analysis of 18S rDNA are: Strain no. 10893 (un identified) from the genera, it is belonging Gymnodinium or Polarella. Strain no. 10795 is closely related other species Cachonina hallii. We tentatively named strain no 11151 and 11160 similar to Gyrodinium or Gymnodinium based on morphology, but these strain indepently position in this tree and is not a real of Gymnodinium sensu stricto. It is possible, we can establish the new genera for strain no. 11151; 11160 because this not cluster any other unarmored species.
KORELASI RAGAM AKTIVITAS TERHADAP KEBERHASILAN MAKAN TIGA JENIS KUNTUL DI CAGAR ALAM PULAU DUA TELUK BANTEN, SERANG Elfidasari, Dewi
Makara Journal of Science Vol. 11, No. 2
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Correlation in Activity Variation to Feeding Success on Three Species Herons at Pulau Dua Nature Reserve Banten's Bays, Serang. This research intent to know activity manner influence that did by three species herons for feeding success at Pulau Dua Nature Reserve, Banten Bay, Serang. Observational method that is utilized is direct observing with backed up by picture taking utilizes observation method is focal. Analysis draws to be done by scan sampling method's. Data analyzed by correlation terminologically Spearmann by programs SPSS. Result observationally to point out that activity type was done upon eating is step, strike, catch and devours prey. Result tests Spearmann's correlation proves that activity manner a large part gets positive correlation with feeding success for three species herons depend on different feeding location. Just activity stop and not active just that gets negative correlation with feeding success on three species herons
VARIABILITAS SUHU DAN SALINITAS DI PERAIRAN CISADANE Hadikusumah, Hadikusumah
Makara Journal of Science Vol. 11, No. 2
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The Temperature and Salinity Variabilities at Cisadane Estuary. The study was conducted at Cisadane Estuary at 18 oceanographic station in Transition Monsoon Season I, East Monsoon Season, and Transition Monsoon Season II from 2003 to 2005. The area of the study was located at the longitude of 106.58° - 106.70° E and the latitude of 5.96° - 6.02°S. The measurements of temperature, salinity, tubidity and light transmision used CTD (Conductivity, Temperature and Depth) Model SBE-19. The result shows that the temperature and salinity vertical profil variabilities at Cisadane Estuary underwent a change in the influence of Transition Monsoon Season I, East Monsoon Season, and Transition Monsoon Season II, for example it was obtained the leg time of the maximum salinity of Transition Monsoon Season II as the same as that of East Monsoon Season. Based on the horizontal and vertical distribution pattern analysis of the interaction between low salinity fresh water of Cisadane River and high salinity sea water of Java Sea, it was also influenced by the season variability and tide. The surface layer was much more influenced by the low salinity and the heat of sunray (seasonal variability) with the weaker intensity to the lower layer. The change of the heat energy by the increase of seasonal temperature occurred in September 2003 to May 2004 ((∆E = 600.6 ⋅ 105 Joule), July to November 2005 (∆E = 84.9 Joule). The decrease of the heat energy occurred in June to September 2003 ((-267.6 ⋅ 105 ), May ke October 2004 (∆E = 189.3 ⋅ 105 Joule) and October 2004 to July 2005 (∆E = -215.4 ⋅ 105 Joule).
PENGAMATAN KADAR MERKURI DI PERAIRAN TELUK KAO (HALMAHERA) DAN PERAIRAN ANGGAI (PULAU OBI) MALUKU UTARA Edward, Edward
Makara Journal of Science Vol. 11, No. 2
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Observation on Mercury Concentration in Kao Bay (Halmahera) and Anggai Waters (Obi Island), North of Maluku. Observation on merkuri concentration in territorial waters of Kao Bay and Anggai have been done in January 2005. The result showed that mercury concentration in seawater and sediment in territorial waters of Kao Bay and Anggai waters still lower and still in line with peaceful threshold value for waters biota life. This data indicate that waste of mercury coming from activity of gold mining by PT Nusa Halmahera Raya (NHR) and illegal gold mining by society not yet polluted both of that waters.
ISOLASI DAN PENGKLONAN FRAGMEN cDNA DARI GEN PENYANDI MULTIDRUG RESISTANCE ASSOCIATED PROTEIN DARI Melastoma affine Suharsono, Suharsono; Firdaus, Syarifin; Suharsono, Utut Widyastuti
Makara Journal of Science Vol. 11, No. 2
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Isolation and Cloning of cDNA Fragment of Gene Encoding for Multidrug Resistance Associated Protein from M. affine. M. affine can grow well in acid soil with high level of soluble aluminum. One of the important proteins in the detoxifying xenobiotic stress including acid and Al stresses is a multidrug resistance associated protein (MRP) encoded by mrp gene. The objective of this research is to isolate and clone the cDNA fragment of MaMrp encoding MRP from M. affine. By reverse transcription, total cDNA had been synthesized from the total RNA as template. The fragment of cDNA MaMrp had been successfully isolated by PCR by using total cDNA as template and mrp primer designed from A. thaliana, yeast, and human. This fragment was successfully inserted into pGEM-T Easy and the recombinant plasmid was successfully introduced into E. coli DH5α. Nucleotide sequence analysis showed that the lenght of MaMrp fragment is 633 bp encoding 208 amino acids. Local alignment analysis based on nucleotide of mRNA showed that MaMrp fragment is 69% identical to AtMrp1 and 63% to AtMrp from A. thaliana. Based on deduced amino acid sequence, MaMRP is 84% identical to part of AtMRP13, 77% to AtMRP12, and 73% to AtMRP1 from A. thaliana respectively. Alignment analysis with AtMRP1 showed that MaMRP fragment is located in TM1 and NBF1 domains and has a specific amino acid sequence QCKAQLQNMEEE
ADSORPTION OF WATER AND BENZENE VAPOUR IN MESOPOROUS MATERIALS Paulina, Paulina
Makara Journal of Science Vol. 11, No. 2
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Mesoporous materials have attracted the attention of many researchers due to the potential applications promised by the materials. This article discusses adsorption of water and benzene vapour in mesoporous materials (mesoporous silica: MCM-41, MCM-48 and their modification). MCM-41 and MCM-48 were synthesized hydrothermally at 100 o C using cethyltrimethylammonium chloride or dodecyltrimethylammonium bromide for MCM-41 (C16) or MCM-41 (C12) respectively and a mixture of cethyltrimethylammonium bromide and Triton X-100 for MCM-48 as templates. Their modifications were conducted by silylation of MCM-41 (C16) and MCM-48 with trimethylchloro silane (MCM16- TMCS and MCM48-TMCS) and t-butyldimethylchloro silane (MCM16-TBDMCS and MCM48-TBDMCS). Results showed that MCM-41 and MCM-48 materials had hydrophobic features which were shown in the small amount of water adsorption at low P/P0 . The hydrophobicity of samples used in this study decrease in the sequence: MCM-41 (C16) > MCM-48 > MCM-41 (C12). The hydrophobicity increased when MCM-41 and MCM-48 were silylated with TMCS or TBDMCS. All unsilylated MCM materials show higher affinity to benzene at low P/P0 than the silylated samples. The results of water and benzene adsorption showed that silylated samples are promising candidates as selective adsorbents for organic compounds.
KOMPAKTIBILITAS KOMPOSIT ISOTROPIK Al/Al2O3 DENGAN VARIABEL WAKTU TAHAN SINTER Widyastuti, Widyastuti; Siradj, Eddy S; Priadi, Dedi; Zulfia, Anne
Makara Journal of Science Vol. 11, No. 2
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Compactibility of Al/Al2O3 Isotropic Composite with Variation of Holding Time Sintering. The requirement of component with structural ability, light weight and also strength is increasing base on Metal Matrix Composites (MMCs) by aluminum as matrix (AMCs). A structural ability is connected to composites compactibility which is depend on quality of interfacial bounding. Powder metallurgy is one of method to produce composite with powder mixing, compacting and sintering. Volume fractions reinforced and sintering time can influence composites compactibility. Volume fractions reinforced variable can produce different reinforcement effect. Beside that, on sintering enables the formation of new phase during sintering time. In this research, Al/Al2O3 isotropic composites are made with aluminum as matrix and alumina (Al2O3) as reinforced. Volume fraction reinforced used 10%. 20%. 30% and 40%. Sintering temperature and compaction pressure are each 600o C and 15 kN. The tests that applied are compression and metallographic test. The result that obtained is optimum compactibility of Al/Al2O3 composite reached at holding time 2 hour. During sintering, new phase can occur that is aluminum oxides (alumina), with unstable properties. The best volume fraction reinforced and holding time sintering are 40% and 2 hours.

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