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Ivandini Tribidasari A.
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Makara Journal of Science
Published by Universitas Indonesia
ISSN : 23391995     EISSN : 23560851     DOI : https://doi.org/10.7454/mss
Core Subject :
Makara Journal of Science publishes original research or theoretical papers, notes, and minireviews on new knowledge and research or research applications on current issues in basic sciences, namely: Material Sciences (including: physics, biology, and chemistry); Biochemistry, Genetics, and Molecular Biology (including: microbiology, physiology, ecology, taxonomy and evolution); and Biotechnology.
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Articles 880 Documents
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PURIFIKASI DAN PENCIRIAN ENZIM PROTEASE FIBRINOLITIK DARI EKSTRAK JAMUR MERANG
Makara Journal of Science
Publisher : UI Scholars Hub

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Purification and Characterization of Fibrinolytic Proteases from Mushroom Volvariela volvaceae Extract. Edible straw mushroom (V. volvaceae) has been known used for improvement of blood circulation due to its fibrinolytic content. The objective of the study is to purify and characterize fibrinolytic protease from straw mushroom extract. Purification were performed through several steps, i.e. precipitation using ammonium sulphate 75%, dialyzed membran (cut-off 10 kDa), and ion-exchange chromatography using DEAE Sepharose. The active fraction of DEAE-Sepharose contains two purified protein bands with molecular weight of 12.9 and 15.8 kDa. The active fraction has specific activity of 0.383 U/mg with 2.7 fold higher purification compared to its crude extract. Both crude and purified enzymes had optimum activity at temperature of 50 ºC and pH 7 in 10 minutes of incubation. Fibrin zymographic profile demonstrated that the enzyme hydrolyzed fibrin, as well as casein, indicating their potent fibrinolytic activity. The enzyme was strongly inhibited by phenilmethylsulphonyl fluoride and N-p-tosil-L-lysinchloromethyl keton. This suggested that it was a serine protease. In summary, these results showed that crude and purified protease of straw mushroom (V. volvaceae) has fibrinolytic activities that can be applied for alternative thrombolytic therapy
ISOLASI FRAGMEN cDNA DARI GEN PENYANDI AKTIN DARI Melastoma malabathricum
Makara Journal of Science
Publisher : UI Scholars Hub

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Isolation of cDNA Fragment of Gene Encoding for Actin from Melastoma malabthricum. M. malabathricum grows well in acidic soil with high Al solubility, thereby it can be used as a model plant for tolerance to aluminum and acid stresses. Actin is housekeeping gene used as an internal control for gene expression analysis. The objective of this research was to isolate and clone the cDNA fragments of MmACT encoding for actin of M. malabathricum. Total RNA was isolated and used as the template for cDNA synthesis by reverse transcription. Four cDNA fragments of MmACT, called MmACT1, MmACT2, MmACT3, and MmACT4, had been isolated and inserted into pGEM-T Easy plasmid. Nucleotide sequence analysis showed that the size of MmACT1 and MmACT2 is 617 bp, whereas MmACT3 and MmACT4 is 735 bp. The similarity among these four MmACT is about 78%-99% based on nucleotide sequence and about 98%-100% based on amino acid sequence. Phylogenetic analysis based on amino acid sequence showed that at 1% dissimilarity, the MmACT1, MmACT2, MmACT3 and the ACT5 Populus trichocarpha are clustered in one group, while the MmACT4 is grouped with ACT9 P. trichocarpa and ACT1 Gossypium hirsutum, and these two groups are separated from actin group of monocotyledonous plants. The sequence of MmACT fragments were registered in GenBank/EMBL/DDBJ database with accession numbers AB500686, AB500687, AB500688, and AB500689.
DETEKSI INTEGRITAS GENOMIK PISANG HASIL IRADIASI IN VITRO BERDASARKAN PENANDA MIKROSATELIT
Makara Journal of Science
Publisher : UI Scholars Hub

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Genomic Integrity Detection of In Vitro Irradiated Banana Using Microsatellite Marker. The research aims to detect genomic integrity of in vitro irradiated banana using microsatellite marker. These studies were done on banana cv. Pisang Mas irradiated by 15 Gy of gamma ray. The DNA was isolated from each accesion following Dixie. Amplification of DNA products were done by Perkin Elmer Gene Amp PCR 2400 using ten primers, and then electroforesis in agarose 1%. Finally a vertical polyacrylamide gel electroforesis was run and the products were visualized by silver staining. The result shown that among the primers tested, eight primers produced clear, discrete, and reproducible bands. Number of DNA band exhibited ranging from one to two, following the ploidy level of pisang Mas which is a diploid banana cultivar (AA). One band suggest homozygote allele while two bands showed heterozygote allele. Out of eight primers, six primers produced different allele among irradiated, in vitro, and in vivo control plant. Meanwhile, for the other two primers the allele were monomorph for all the accessions examined. Genomic modification was observed at all irradiated plants. The modification can happened at zygosity of certain allele that may change from heterozygote to homozygote or vice versa. While modification in allele size that underlying genomic instability could be caused by several genetic events such as deletion, insertion, and amplification of nucleotides.
PROPERTIES OF HIGH-ORDER HARMONIC GENERATION SIGNALS FROM SWITCHED-OFF ALIGNED MOLECULES
Makara Journal of Science
Publisher : UI Scholars Hub

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PENURUNAN NOISE FIGURE PERFORMANCE (FN) PADA HETEROJUNCTION BIPOLAR TRANSISTOR SI/SI 1-X GEX BERDASARKAN PENGATURAN STRIPE EMITTER AREA (AE) DAN FRACTION MOLE (X)
Makara Journal of Science
Publisher : UI Scholars Hub

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Reduction of Noise Figure (Fn) on Bipolar Heterojunction Si/Si1-x Gex based on Arrangements Stripe Emitter Area (Ae) and Mole Fraction (x). This paper investigates the influence of stripe emitter area (Ae) and fraction mole (x) concerning about noise figure performance (FN) at HBT SiGe. It takes the second generation of HBT SiGe IBM with Ae; 0.18 x 10 μm2 developed as its model. When the alterations of Ae are from 0.18 x 10 μm2 , 0.12 x 10 μm2 and 0.09 x 10 μm2 , at the same time the fraction mole (x) is raised twice, it makes positive relation to RB and β parameter, but negative relation to RC, fT, and fmax parameter. A model of HBT SiGe with x: 0.1 and Ae: 0.18 x 10 μm2 produces 0.57dB of Fn minimum, the value is less than the other of which has 0.64dB for Ae: 0.12 x 10 μm2 and 0.69 dB for Ae: 0.09 x 10. Then if fraction mole(x) is reduced for 50%, it causes nonlinear Fn raising which are 77%, 79% and 89% from the previous value. Noise figure (Fn) had a negative relationship with emitter stripe area (Ae), whereas the mole fraction (x) has a positive relationship; so the noise figure (Fn) can be made low by minimizing emitter stripe area (Ae) and raise the mole fraction (x).
IDEAL MAKSIMAL DAN PRIMA DARI GELANGGANG POLINOM MIRING ATAS DAERAH BILANGAN BULAT GAUSS
Makara Journal of Science
Publisher : UI Scholars Hub

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Maximal and Prime Ideals of Skew Polynomial Ring Over the Gauss Integers Domain. Let R be any ring with identity 1, σ be an automorphism of R and δ be a left σ-derivation. The skew polynomial ring over R in an indeterminate x is the set of polynomials anx n + an-1x n-1 + . . . + a0 where ai∈ R with multiplication rule xa = σ (a) x + δ(a) for all ai∈ R. In this paper, R is Gauss integers, i.e Z + Zi, where i 2 = -1, σ is the automorphism of R with σ(a + bi) = a - bi where a,b∈ Z, the ring of integers, and δ is the zero σ-derivation. We will show maximal and prime ideals of this skew polynomial ring.
COMPUTATION OF NATURAL CONVECTION IN A POROUS PARALLELOGRAMMIC ENCLOSURE WITH A MAGNETIC FIELD
Makara Journal of Science
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Detailed numerical calculations are presented in this paper for natural convection in a porous parallelogrammic enclosure with a magnetic field. The inclined walls are maintained isothermally at different temperatures. The top and bottom horizontal straight walls are kept adiabatic. To simplify the effort in matching the grid mesh with the inclined walls of the parallelogrammic region/geometry, the computational domain is mapped onto a rectangular shape using a non-linear axis transformation. Transport equations are modeled by a stream-vorticity formulation then expressed in the new coordinate system and solved numerically by a finite difference method. Based upon the numerical predictions, we found the convection modes within the enclosure depended upon the Rayleigh number and the inclination angle. As the value of magnetic field is made larger, the strength of the heat transfer is progressively suppressed. Tuning the inclination angle decreases the heat transfer performance.
PERSONALIZATION SISTEM E-LEARNING BERBASIS ONTOLOGY
Makara Journal of Science
Publisher : UI Scholars Hub

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TOKSISITAS AKUT DAUN Justicia gendarussa Burm
Makara Journal of Science
Publisher : UI Scholars Hub

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Acute Toxicity of Justicia gendarussa Burm. Leaves. Preminelary experiment showed that ethanolic extract of gandarusa leaves (Justicia gendarussa Burm.) could decreased uric acid blood level on rats. The aim of this experiment was to determine of the value LD50 and liver function based on activities of aminotransferase. Animals test which were used in this experiment were 50 males and 50 females white mice. They were divided into 5 groups. Group 1 as control group was given aquadest. Group 2-5 were treated by ethanolic extract of gandarusa leaves with dosage 4, 8, 16, and 32 g/kg bw. The LD50 value was determined by the amount of death in group during 24 hours after giving a single dose of test substance. The result showed that the highest dose was practically non toxic with LD50 value of 31.99 g/kg bw (male groups) and 27.85 g/kg bw (female groups). Measurement of aminotransferase activity was done by using colorimetric method. The result of ANOVA analysis for liver function showed that the giving test substance 4 g/kg bw –16 g/kg bw was not significantly different between treated groups and control group.
KERAGAMAN GENETIK PANDAN ASAL JAWA BARAT BERDASARKAN PENANDA INTER SIMPLE SEQUENCE REPEAT
Makara Journal of Science
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Genetic Diversity of Pandanus from West Java based on ISSR Markers. The genetic diversity of 10 species of Pandanus from West Java was examined using inter simple sequence repeat (ISSR) technique. Samples were collected from different localities in West Java. Two primers (ISSR2 and ISSR7) were selected as reliable amplifying ISSR markers. The two primers amplified 19 band position, with amplification size ranged from 200 to 1500 bp. Genetic distance for samples ranged from 0.267 to 0.957. Genetic distance was high (0.957) between P. pseudolais and P. spinistigmaticus, and between P. kurzii and P. pseudolais, where as genetic distance was low (0.267) between P. scabrifolius and P. bidur.

Page 37 of 88 | Total Record : 880

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