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Ivandini Tribidasari A.
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Makara Journal of Science
Published by Universitas Indonesia
ISSN : 23391995     EISSN : 23560851     DOI : https://doi.org/10.7454/mss
Core Subject :
Makara Journal of Science publishes original research or theoretical papers, notes, and minireviews on new knowledge and research or research applications on current issues in basic sciences, namely: Material Sciences (including: physics, biology, and chemistry); Biochemistry, Genetics, and Molecular Biology (including: microbiology, physiology, ecology, taxonomy and evolution); and Biotechnology.
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Articles 880 Documents
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TROUBLESHOOTING IN EXPRESSION AND PURIFICATION OF RECOMBINANT SEVERE ACUTE RESPIRATORY SYNDROME-ASSOCIATED CORONAVIRUS NUCLEOCAPSID PROTEIN IN Escherichia coli BL21
Makara Journal of Science
Publisher : UI Scholars Hub

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EVALUASI SKEMA WATERMARKING CITRA BERBASIS SINGULAR VALUE DECOMPOSITION, KUANTISASI DITHER, DAN DETEKSI SISI
Makara Journal of Science
Publisher : UI Scholars Hub

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CRYSTAL STRUCTURE OF [Bis(DIMETHYLFORMAMIDE) Bis(2,2’-PYRIDYL)QUINOLINE)IRON(II)]Bis-(TETRAPHENYLBORATE)
Makara Journal of Science
Publisher : UI Scholars Hub

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The goal of this research is to obtain single crystal and structural information of iron(II) complex with 2,(2’- pyridyl)quinoline(pq) ligands. The reaction of iron(II) salt with 2,(2’-pyridyl)quinoline ligand and sodium tetraphenylborate in the molar ratio of 1:3:2 in methanol-N,N-dimethylformamide(dmf) solution results in an iron(II) complex. The formula of the [Fe(pq)2(dmf)2](BPh4)2 complex has been obtained from the iron(II) and C, H, N contents. Single crystal of [Fe(pq)2(dmf)2](BPh4)2 suitable for X-ray investigation was obtained by evaporation of the complex solution in N,N-dimethylformamide at room temperature after 24 hours. This compound crystallizes in monoclinic system with C2/c space group, a = 27.950(4), b = 14.169(7), c = 17.717(9) Å and β = 105.669(11)°. The structure consist of iron(II) is chelated by two pq ligands through the N atoms and two dmf molecules in a six-coordination environment. The charge of the [Fe(pq)2(dmf)2] 2+ cation is balanced by two tetraphenylborate (BPh4) - anions.
BIODIESEL FROM LOW GRADE USED FRYING OIL USING ESTERIFICATION TRANSESTERIFICATION PROCESS
Makara Journal of Science
Publisher : UI Scholars Hub

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Biodiesel from Low Grade Used Frying Oil Using Esterification Transesterification Process. The aim of this research was to obtain the optimal condition for the production of biodiesel from low grade used frying oil through the esterification transesterification process. The experiment was arranged in Completely Randomized Design with Duncan’s advanced test. Results showed that the biodiesel FFA content is ranged between 0.167 to 0.795% (P
PRODUKSI GAS HASIL BIODEGRADASI MINYAK BUMI: KAJIAN AWAL APLIKASINYA DALAM MICROBIAL ENHANCED OIL RECOVERY (MEOR)
Makara Journal of Science
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Gas Production Generated from Crude Oil Biodegradation: Preliminary Study on its Aplication in Microbial Enhanced Oil Recovery (MEOR). The objective of this study is to observe the capacity of gas production generated from crude oil degradation by the isolated bacteria. The gas in the MEOR could increase pressure in the reservoir, decrease oil viscosity, increase oil permeability-due to the increase of the porosity and viscosity, and also increase oil volume due to the amount of dissolved gas. A research on gas analysis of oil degradation by 6 isolated bacteria has been conducted. The bacteria isolates including Bacillus badius (A), Bacillus circulans (B), Bacillus coagulans (C), Bacillus firmus (D), Pasteurella avium (E) and Streptobacillus moniliformis (F). The trial on gas production, gas analysis and oil degradation analysis, was carried out by using SMSS medium. The test of gas production was done by using microrespirometer at 40°C. The result shows that B, C, D, E produce more gas than A and F. Gas of CO2, O2, CO, N2, CH4, and H2 were analyzed by using GC. The results show that only three gases were detected by GC i.e. CO2, N2, and O2. The concentration of CO2 and N2 gas increased while the concentration of O2 decreased over an 8th day of observation. CO2 gas producted by mix culture was higher than by the pure culture. On the 8th day of incubation, the production of CO2 gas by mix culture was 4,0452% while pure culture C and D only produced 2,4543% and 2,8729%. The mix culture increase simple hydrocarbon by 12.03% and the formation of a complex hydrocarbon by 3.07%. The mix culture (C-D) generated the highest concentration of CO2 gas as well as a synergistic concortium that has ability to degrade crude oil.
TINGKAT PENCEMARAN LOGAM BERAT DALAM AIR LAUT DAN SEDIMEN DI PERAIRAN PULAU MUNA, KABAENA, DAN BUTON SULAWESI TENGGARA
Makara Journal of Science
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The Degree of Heavy Metals Pollution in Seawater and Sediment in the Waters of Muna, Kabaena and Buton Island, Norteast of Sulawesi. Muna, Kabaena, and Buton are islands found in Northeast of Sulawesi. One of them, namely Kabaena Island is island which has high Nickel content. Some of mining industries has explored the Nickel content in this area. Tailing come from mining thrown to the sea, will increase the heavy metals content in seawater and sediment in the waters of that third island, remembering the position of that third island not so far. In order to marine pollution monitoring by heavy metals, Research Center for Oceanography has done some marine research in the waters of that third island in April 2006. The aim of this research is to know the impact of Nickel mining activities to heavy metals content in seawater and sediment in that third waters. Heavy metals monitored is Pb, Cd, Cu, Zn, and Ni. The results showed that the content of heavy metals Pb, Cd, Cu, Zn, and Ni in sea water still in line with the threshold value according to Kep MNLH No. 51, 2004 for sea biotas. The same condition also found in sediment, where the content of Pb, Cd, Cu, Zn, and Ni is low relative, and still in line with heavy metals content found in normal seawater.
OPTIMASI BIODEGRADABILITAS DAN UJI TOKSISITAS HASIL DEGRADASI SURFAKTAN LINEAR ALKILBENZENA SULFONAT (LAS) SEBAGAI BAHAN DETERJEN PEMBERSIH
Makara Journal of Science
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Optimization of Biodegradability and Toxicity Testing of Degradation Product from Linear Alkyl Benzene Sulfonate (LAS) Surfactant as Cleaning Detergent Agent. Linear Alkyl Benzene Sulfonate (LAS) is a surfactant used in laundry detergent as cleaning agent and toxic to aquatic organisms. Results shows, with the LAS concentration used (20 ppm) in medium, adaptation time and Acinetobacter sp. growth has shown better biodegradation ability than three other bacteria used Pseudomonas putida, Pseudomonas fluorescence, Bacillaria spp. Thus, Acinetobacter sp is used further for biodegradation process of LAS. Based on its biodegradation half-life using Acinetobacter sp, and with mixed culture (± 52.32% and ± 46.82% respectively) could be achieved in 4 (four) days, LAS could be categorized as a biodegradable compound. The toxicity assay is based on tetrazolium dye reduction with Rhizobium meliloti as indicator organism. LAS is more toxic than its intermediate product from biodegradation, with IC50 = 34.35 ppm, and the intermediate product, Ac and Cm, has IC50 = 446.19 ppm and 111.28 ppm respectively. Identification of intermediate products using IR and LC-MS analysis shows that the degradation product contains chemicals compounds with functional group as follows: benzene, benzoic acid, hydroxyl, and aliphatic carbons with large molecule weight. Until its half-time degradation time, LAS biodegradation process only occurs at the aliphatic carbon chain, and have not yet reached the stage of aromatic ring opening.
APPLICATION OF HYDROXYAPATITE IN PROTEIN PURIFICATION
Makara Journal of Science
Publisher : UI Scholars Hub

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The precursors, Na2HPO4.2H2O and CaCl2.2H2O are used for synthesizing pure hydroxyapatite which less of carbonate content. The high temperature of sintering, about 700o C of temperature, is treated to minimize the carbonate group on hydroxyapatite surface. Carbonate content of hydroxyapatite which is sintered in 700o C is less than 110o C. It indicates an increasing temperature of sintering will increase crystallinity and decrease carbonate content of hydroxapatite. This method gave better way to resulting hydroxyapatite crystal. The patterns of band of SDS PAGE in resulting of Protein Purification by DEAE matrix is also appear in using hydroxyapatite matrix. The other band also appear in purification by hydroxyapatite matrix, it showed that hydroxyapatite not only has DEAE matrix characteristic as anion exchange but also has kation exchange characteristic. These patterns proof that hydroxyapatite could be used in protein purification as matrix as cation an anion exchange. Additionally, apatite matrix is abiding and can be used repeatedly more than one hundred times without contamination.
KARAKTERISTIK GELOMBANG DAN ARUS DI ERETAN, INDRAMAYU
Makara Journal of Science
Publisher : UI Scholars Hub

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The Wave and Current Characteristic at Eretan, Indramayu. Erosion process occurs in many places, but each of these processes have their respective characteristics. The purpose of research for understanding the dynamics of the process that caused the coastline of the interaction between air, sea and land. Research has been conducted in Eretan, Indramayu in 2006. The results show the average wave height (H1/3) in February obtained higher (60.4 cm) compared with August (23.6 cm) and in May obtained a much lower (5.7 cm). Current velocity in February and in August obtained dominant raised due to by field of wave and the influence of tidal currents do not exist. Instead current velocity obtained in May was raised by the dominant tidal currents and very small currents generated by the wave field. The influence of the wave field to the velocity and direction of flow will distribute sediment transport in the area of wave breack (breack water) to the coastline. Characteristic wave has a period of 3, 5, and 7 second is the trigger shoreline retreat 1.5 m/year.
DETEKSI PEMALSUAN CITRA BERBASIS DEKOMPOSISI NILAI SINGULIR
Makara Journal of Science
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Image Fakery Detection Based on Singular Value Decomposition. The growing of image processing technology nowadays make it easier for user to modify and fake the images. Image fakery is a process to manipulate part or whole areas of image either in it content or context with the help of digital image processing techniques. Image fakery is barely unrecognizable because the fake image is looking so natural. Yet by using the numerical computation technique it is able to detect the evidence of fake image. This research is successfully applied the singular value decomposition method to detect image fakery. The image preprocessing algorithm prior to the detection process yields two vectors orthogonal to the singular value vector which are important to detect fake image. The result of experiment to images in several conditions successfully detects the fake images with threshold value 0.2. Singular value decomposition-based detection of image fakery can be used to investigate fake image modified from original image accurately.

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