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Journal of Biodiversity and Biotechnology
Published by Universitas Sebelas Maret
ISSN : 27755223     EISSN : 28083229     DOI : https://doi.org/10.20961/jbb
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Journal of Biodiversity and Biotechnology is a scientific journal focused on biodiversity and biotechnology published twice a year (June and December) managed and published by Universitas Sebelas Maret. This journal covers original research in the following areas: biodiversity and environment, biotechnology, agriculture biotechnology, food biotechnology, medical biotechnology, bioinformatics, molecular biology, biochemistry, bioinformatics, phyto-pharmacy, herbal medicine, and animal science. Other topics are welcome if it discusses about biodiversity or biotechnology.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Agronomi dan Ilmu Tanaman
Articles 6 Documents
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Search results for , issue "Vol 3, No 2 (2023)" : 6 Documents clear
Effect of Auxin and Cytokinin on Orchid Subculture Resulting from Coelogyne Crosses Sri Hartati; Ahmad Yunus; Iistikharoh Iistikharoh
Journal of Biodiversity and Biotechnology Vol 3, No 2 (2023)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v3i2.80626

Abstract

The black orchid or Coelogyne is one of the native orchids of Kalimantan, widely cultivated in Indonesia. Several species of Coelogyne orchids have been crossed to obtain new superior-quality plants. Modifying the media, such as giving PGR, is necessary for good plant multiplication results. This research was conducted by changing MS growing media with ZPT BA and NAA. This experiment aimed to determine the effect of ZPT BA and NAA on the growth of orchid subcultures from a cross between Coelogyne pandhurata and Coelogyne rumphii. The study used a completely randomized design (RAL) with 2 factors, namely ZPT BA and NAA, each having a concentration of 0 ppm, 0.2 ppm; 0.4 ppm, and 0.6 ppm with 16 treatment combinations and 4 replications. They were implemented at the Laboratory Plant Physiology and Biotechnology, Faculty of Agriculture, UNS Surakarta, from November 2020 to March 2021. The variables observed included the number of shoots, plant length, plant weight, number of leaves, number of roots, root length, when roots appeared, and when shoots appeared. The results showed that BA 0.6 ppm gives the best output on the growth of the number of shoots, and NAA concentration of 0,2 ppm gives the best results on the variable number of roots. The combination of BA 0.6 ppm and NAA 0.2 ppm gave the best results on plant weight growth.
Seroprevalence, and Risk Factors of Post-Vaccination Antibodies for Foot and Mouth Disease in Tanah Laut District, South Borneo in 2022-2023 Adrin Ma'ruf; Heru Susetya; Tri Untari; Elfa Zuraida
Journal of Biodiversity and Biotechnology Vol 3, No 2 (2023)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v3i2.74595

Abstract

Foot and Mouth Disease (FMD) caused by the Foot Mouth Disease virus (FMDV) is an animal disease that spreads quickly and causes huge economic losses. The Indonesian government has established the vaccination program as a form of national FMD management in 2022. This study was conducted to determine the success and post-vaccination seroprevalence of FMD in Tanah Laut District, South Borneo, and to analyze the factors that influence the success of vaccination. The research method is observational analytic with a cross-sectional study approach. Samples were sera of cows and goats that had been vaccinated, then tested serologically with SP ELISA serotype O and NSP ELISA. Result analyzed by chi-square test (X2), odds ratio (OR), relative risk (RR), and spatial analysis (average nearest neighbor).The seroprevalence of immunity to FMD in Tanah Laut Regency was 95.94%. Factors that influence the formation of antibodies after FMD vaccination were the type of animal (OR = 3.781), age (OR = 6.106), sex (OR = 2.801), rearing system (OR = 3.848), feed (OR = 3.448), type of vaccine (OR = 5.508), and number of vaccinations (OR = 0.109). Spatial analysis shows a clustered pattern (Nearest Neighbor Ratio = 0.005457). The seroprevalence of immunity to FMD in Tanah Laut Regency was 95.94%. Goat immune seroprevalence has a higher value when compared to cattle. Factors that influence the formation of FMD vaccination antibodies are animal type, age, sex, feed, rearing system, type of vaccine, and number of vaccinations.
Preparation of Biopesticide Powder from Jicama (Pachyrhizus erosus) Leaf Extract to Eradicate Whitefly Pests (Bemisia tabaci G.) on Eggplant Plants (Solanum melongena L.) Laila Silvy Fatmawati; Natasya Dian Andini; Dr. Margono
Journal of Biodiversity and Biotechnology Vol 3, No 2 (2023)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v3i2.83481

Abstract

The use of synthetic pesticides in Indonesia is increasingly widespread because they are considered effective in killing pests. However, synthetic pesticides leave residues that can cause environmental pollution and human health problems. Therefore, another alternative is needed, namely switching to using environmentally friendly vegetable pesticides. One of the alternatives is biopesticide powder from jicama leaf extract as a whitefly pest controller. Pomace leaves contain active compounds that are toxic to whitefly, namely rotenone which is a group of flavonoid compounds. The method of making this biopesticide powder consists of 2 stages, the first is the preparation of jicama leaf extract through a maceration process for 2 days with the ratio of leaf powder and solvent, namely 40 g, 60 g, 80, and 100 g of jicama leaf powder in 500 ml of 96% ethanol. The second stage is the processing of jicama leaf extract into biopesticide powder through the encapsulation process, namely mixing the extract with maltodextrin using a weight ratio of extract maltodextrin, namely 1 : 2 (w : w). The analysis carried out was the test of biopesticide effectiveness against whitefly. After testing, it was found that the biopesticide powder that was effective in eradicating whitefly pests was a biopesticide powder with a ratio of 100 grams of jicama leaf powder in 500 mL of 96% ethanol with a dissolution dose of 20 g/L in water, which was able to kill whitefly with a mortality percentage of 100% on day 3.
Novelty, Distinctiveness, Uniformity, and Stability Testing for Three Potential Lines of Black Rice Nandariyah - Nandariyah; Yuyun Nitasari; Bambang Pujiasmanto; Endang Yuniastuti
Journal of Biodiversity and Biotechnology Vol 3, No 2 (2023)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v3i2.80676

Abstract

Black rice Cempo Ireng is a local variety with a long plant life, high plant posture, and low productivity. Plant breeding by gamma irradiation was carried out to correct these weaknesses. The results of the gamma irradiation process included a selection process for each generation and produced potential lines numbers such as 8, 51, and 52. The characterization was based on the NDUS (Novelty, Distinctiveness, Uniformity, and Stability) test. The purpose of this study was to characterize potential lines as one of the requirements for the release of plant varieties. The study was conducted in the paddy fields of Pakahan Village, Jogonalan District, Klaten Regency, from February-July 2022. The method used was a single factor randomized complete block design with three replications. Data were analyzed by T test with 5% level. The results of the study showed that the three potential lines have differences with the parent and the Sembada varieties. Three potential lines can be deemed to be new, dictinct, uniform, and stable.
DNA Damage in Prader Willi Syndrome Against Obesity Diennisa Mursalin
Journal of Biodiversity and Biotechnology Vol 3, No 2 (2023)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v3i2.77619

Abstract

Prader Willi Syndrome is a multisystem genetic disorder caused by deletions of paternal chromosomes (75%), maternal chromosomal dysomi (25%), and translocations (1%). This syndrome has a main characteristic, namely the state of obesity in individuals sufferers where. This is due to deficiency of Growth Hormone levels due to central hypothalamic-limbic disorders that occur in this syndrome. This syndrome has a characteristic course of obesity in every phase of life. The obesity that is characteristic of this syndrome causes diverse complications because it causes DNA damage through chronic inflammation caused by an increase in pro-inflammatory cytokines due to the buildup of adipose tissue. Thus, individuals with PWS can experience various metabolic diseases that can increase their morbidity and mortality.
Application of ZA Fertilizer on Growth and Production of Red Ginger (Zingiber officinale var. Rubrum) Albriansyah Zinka Ancoro Putro; Supriyono Supriyono; Sulandjari Sulandjari; Djoko Purnomo
Journal of Biodiversity and Biotechnology Vol 3, No 2 (2023)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v3i2.74442

Abstract

Red ginger is one of the export commodities of spices in Indonesia which plays a significant role in the country's foreign exchange earnings. In order to meet market demand, ginger cultivation needs to be fertilized both organically and inorganically to increase production. ZA fertilizer for plants serves to stimulate vegetative growth. This study aims to determine the response of red ginger to the application of ZA fertilizer has a significant effect on the growth and yield of red ginger and to obtain the optimum dose of ZA fertilizer for the growth and yield of red ginger. This research was carried out in March - August 2021 until it was completed in Pelem Hamlet, Wonorejo Village, Jatiyoso District, Karanganyar Regency, Central Java Province with coordinates 7°43'24.7”S 111°05'31.2”E. The experimental design was a Completely Randomized Complete Block Design (RCBD). This study consisted of 4 treatments with 6 replications so that 24 experimental units were obtained, while the treatments in this study were as follows: P0: Control without ZA fertilizer, P1: Treatment 225 Kg ZA/ha or 22.5 g/m2, P2: Treatment 450 Kg ZA/ha or 45 g/m2, and P3: Treatment 675 Kg ZA/ha or 67.5 g/m2. The results showed that ZA fertilizer had no significant effect on the yield of red ginger, but on growth observations, P2 treatment with a dose of 450 Kg ZA/ha can have a significant effect on the increase in the number of plants per plot. The dry weight of the stover was strongly correlated with the weight of fresh rhizomes with correlation values (r) 0.508.

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