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Journal of Global Pharma Technology
Published by Universitas Udayana
ISSN : 09758542     EISSN : -     DOI : -
Core Subject : Health,
Medicine & Pharmacology
ournal of Global Pharma Technology is a monthly, open access, Peer review journal of Pharmacy published by JGPT Journal publishes peer-reviewed original research papers, case reports and systematic reviews. The journal allows free access to its contents, which is likely to attract more readers and citations to articles published in JGPT. JGPT publishes original research work that contributes significantly to the scientific knowledge in pharmacy and pharmaceutical sciences- Pharmaceutics, Novel Drug Delivery, Pharmaceutical Technology, Cosmeticology, Biopharmaceutics and Pharmacokinetics, Pharmacognosy, Natural Product Research, Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmaceutical Analysis, Pharmacology, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics, Biotechnology and Applied Computer Technology. For this purpose we would like to ask you to contribute your excellent papers in pharmaceutical sciences.
Arjuna Subject : Kedokteran - Onkologi
Articles 1,092 Documents
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Some Biochemical Tests of Broiler Chickens As Affected by Different Levels of Sodium Bicarbonate Kadhim S. Kadhim
Journal of Global Pharma Technology .
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Abstract

The objective of this research to investigate the effect of supplementation of sodium bicarbonate on some biochemical parameters of broiler chickens.  At 42 day-old, broiler chickens were randomly allotted to three dietary treatments, 50 chickes in each fed on diet contain (0%, 0.5% and 1%) of sodium bicarbonate. Then each group was divided in to sub groups, 25 animals in each. At day 42 (the end of the experimental period), chickes were given 5h fasting period and  blood plasma of chicks were collected  without anticoagulant and plasma were separated by centrifugation at 3000 rpm for ten minutes and stored in a deep freez (-20) until analysis serum Carietenini , HDL , Globulins , ALP , ALT , AST , cholesterol , Glucose and total protein were determined by full auto biochemistry technique . As a result , in broiler chickens supplementation of different levels of sodium bicarbonate caused important changes in some biochemical parameters .Significantly positive correlations (P ≤ 0.01) were detected for  HDL ,  ALP , ALT ,and  AST , while (P ≤ 0.05 ) for  Glucose and total protein . It seems, different levels of sodium bicarbonate were no Significant effect on blood Carietenini, Globulins and Cholesterol.Keywords: Sodium bicarbonate, Biochemical traits, Broiler chickens.
Expression relative of RANK, RANKL and OPG gene on Rat Femoral Fracture Healing Process in Delayed Union Model after Pulsed Electromagnetic Field exposure Luluk yunaini
Journal of Global Pharma Technology Volume 11 Issue 04.
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Pulsed electromagnetic field (PEMF) is application medical devices in healing bone fracture. Osteoblast and osteoclast cells have regulation in femoral fracture healing process. PEMF exposure has effect in cell nucleus activity. Genes that have critical role in bone remodelling are Receptor activator of nuclear factor kappa-B (RANK), receptor activator of nuclear factor kappa-B ligand (RANKL) and osteoprotegerin (OPG).This research aimed to analysis effect PEMF exposure in improve bone fracture healing process with delay union model, which its expression level in RANK, RANKL and OPG gene. Our research used experimental design. Sample for this research was callus from rat femoral fracture with delay union. qRT-PCR technique with Livak’s measurement were used to detection expression relative. Statistic analysis used two way ANOVA with a significant differences value p <0.05. The result showed that relative expression on RANK, RANKL and OPG have significance different in PEMF group and times (days) group. We found that the PEMF group can expedite the higher expression relative of RANK, RANKL and OPG than non PEMF group. The result has concluded using PEMF can improve the healing to normal union process on rat with delay union model.
Opportunities for the Prevention of Hypertension by Nutrition Correction Tetiana Nehoda
Journal of Global Pharma Technology Volume 12 Issue 02 (2020) Feb. 2020
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AbstractIntroduction. There is much evidence of a relationship between salt intake and blood pressure levels. Limiting sodium chloride consumption significantly lowers blood pressure. The purpose of this study was to study the effectiveness of lowering blood pressure while limiting salt intake in men and women with high and normal blood pressure.Materials and methods. An annual study was conducted, a prerequisite of which was the selection of 2 groups: survey and comparison. They were comparable in terms of gender, age, professional composition and number of participants. To carry out a dietary examination required limiting the salt in the cooking to 0,5 g per serving.Results and discussion. After one year of dietary intervention, a significant decrease in systolic (SBP) and diastolic blood pressure (DBP) was found in men and women in the intervention group (p < 0,05) compared to the control group, which showed a natural decrease in blood pressure, a significant decrease in blood pressure was observed during the study period (p < 0,05). In addition, there was a significant decrease in body weight in the intervention group compared with the control group.Conclusions. Reduced salt intake significantly reduced SBP and DBP in men and women with high blood pressure over a longer follow-up compared with the control group.
Sperm Detection in Congenital Ejaculatory Duct Cyst Repair: A Case Report Ahmad Ricardo Silalahi
Journal of Global Pharma Technology Volume 10 Issue 12.
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Abstract

Ejaculatory duct cyst is a very rare case. We report a 26-year-old man with primary infertility complaining of aspermia since before marriage, precisely since the first wet dream. General physical examination and hormonal levels were in normal values. The cyst was visualized through abdominal TRUS, MRI and abdominal ultrasound (USG). The sperm detection test was performed before and after repaired by TURED. Before repair, the sperm was absent at Cowper’s gland secretion, post orgasmic urine and post massage prostate. After repair, sperm was detected in cyst aspiration fluid and useful to distinguish ED cyst between the Mullerian duct and utriculus cyst. In addition, after one month post repaired, the sperm concentration, motility and morphology increased. The improvement of sperm quality is useful to determine the infertility program whether IVF, IUI or natural pregnancy.Keywords: Ejaculatory duct cyst, Male infertility, Sperm detection.
Alleviation of Oxidative Stress and Suppression of Inflammatory Cytokines in STZ -induced Diabetes by Banana (Musa paradisiacal), Kiwi (Actinidiadeliciosa planch) and Olive (OleaeuropaeaL.), by-products, Extract: Comparative Study Hanan F. Aly
Journal of Global Pharma Technology Volume 11 Issue 6.
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The effect of peels (kiwi, banana and leaves (olive and banana)acetone extract 80%on streptozotocin (STZ) induced diabetic rats was investigated. Oral administration of 500 mg crude extract /kg body weight for 30 days post induction of diabetes, showed remarkable improvement in all biomarkers under investigation compared to standard drug. Olive leaves showed the highest percentage of improvement in glutathione (GSH), nitric oxide, (NO), glutathione peroxidase (GPX) glutathione –S-transferase (GST) ,catalase, lipid peroxide (MDA) , urea  ,creatinine, tumor necrosis factor- α(TNF-α) interleukin 1β(IL1-β)  (41.74,138.68, 48.77, 26.15, 27.84, 73.54, 97.92, 81.37, 52.96 and 40.24% , respectively).While, banana leaves and kiwi peels extracts showed the highest percentage of improvement in antioxidant enzyme paraoxonase (PON1) and transforming growth factor β(TGF β)reached  16.90 and 41.81%, respectively. From a histopathological point of view, treatment of diabetic rats with different extracts revealed regeneration and amelioration effects of extracts on the pancreas and renal tissues in diabetic rats and apparently normal. It could be concluded that the anti-hyperglycemic properties of plant extracts may offer a potential therapeutic source for the treatment of diabetes. Further work on isolation and characterization of active constituents in these wastes is needed.Keywords: Diabetes, Inflammatory cytokines, Antioxidant, Oxidative stress.
Diagnosis and Management of Interventional Endovascular Carotid-cavernous Fistula: a Retrospective Study Luh Putu Lina Kamelia
Journal of Global Pharma Technology Volume 12 Issue 09 (2020) Sept. 2020
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Carotid-cavernous fistula (CCF) is a consequence of incremental intracavernous pressure. The direction of drainage is usually multidirectional. Initial DSA (digital subtraction angiography) solves in obtaining its information along with advanced technology nowadays. Aim: The study aims to analyze clinical characteristics, cerebral angiographic features, and management of endovascular embolization of CCF. Methods: The study is a retrospective analysis of each patient diagnosed with CCF. They were treated with endovascular embolization from January 2019 to April 2010 at the Brain Center Dr. Wahidin Sudirohusodo (DWS) General Hospital Makassar. We performed DSA on all patients before the endovascular procedure with catheterized access through the right femoral artery. Result: Angiograms of all patients showed typical CCF lesions, 8 patients then received endovascular management by coiling method, 2 were treated with a detachable balloon, while 1 received an Onyx injection. All patients experienced gradual improvement for orbital symptoms. Conclusion: Endovascular embolization-followed cerebral DSA is a useful diagnostic and therapeutic management to improve patients' clinical symptoms with CCF. Keywords: Carotid-cavernous fistula, Digital subtraction angiography, Embolization, Balloon, Onyx.
Hospital-Acquired Urinary Tract Infection, Microbial Causative Agents and Antibiotic Resistance Pattern in Southern Iran: A Prospective Study Farzaneh Dehghan
Journal of Global Pharma Technology .
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Background Objective: Antimicrobial resistance is a problem in hospital-acquired urinary tract infection (UTI), and is the most frequent nosocomial infection. This study was carried out to determine the prevalence of Hospital-acquired Urinary Tract Infection (HAUTI)  a etiological microorganisms and their antimicrobial resistance to commonly used antibiotics. Methods: This cross-sectional study was performed in a referral state general hospital in Bandar abbes, Southern Iran between January, 2014 and December, 2015. All cases   admitted to the different wards   of Shahid Mohammadi Hospital and recognized as symptomatic and asymptomatic   HAUTIs, after 48 h of admission, and those who were readmitted to the hospital because of a UTI up to 48 h after discharge from the hospital were included as the targeted population. According to the checklist, the risk factor and demographic information of these patients were recorded. The identification of isolated microorganisms was done by standard microbiological test, then antimicrobial susceptibility testing of all bacterial isolates was performed by disk diffusion method and statistical analysis was done using SPSS software version 20.0.Results In this study, 1.57% of the 19,768 hospitalized patients   acquired   HAUTI. The most commonly isolated gram negative bacteria  are Escherichia coli 26.7%(82), followed by Acinetobacter baumannii  15.3%(47) and among gram positive organisms, it was Candida spp 23.5%(72). Gram negative bacteria were most resistant to Ceftriaxone, non-fermented bacteria were most resistant to Cefazol in, Cotrimoxazole and Nalidixic acid, Staphylococcus spp were resistant to Ampicillin, Enterococcus fecalis was resistant to Gentamicin and Nalidixic acid while Streptococcus agal actie did not show any resistance pattern .Conclusions The results of this study are indicative of a big challenge about the prevalence of multi-drug resistant bacteria in the studied hospital that limits therapeutic options, if infection con­trol policy and continuous monitoring of ESBL bacteria are not used.Keywords: Antibiotic resistance, Hospital-acquired Urinary Tract Infection.
Green Chemistry, Characterization and Study of Medical Application Silver/ Polymer Nanocomposite Khalidah Khalaf Jabbar
Journal of Global Pharma Technology Volume 10 Issue 12.
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Over the last few years, the green consistency over nanoparticles (NPs) the usage of drive into extracts has emerged as a hopeful methodology because of the fabrication of metal NPs (especially silver, copper, and riches NPs), so kind of it entails an easy, fast, low-cost, then environmentally pleasant bioprocess. The consistency atop nanoparticles through the use of the biochemical rejoinder regarding propulsion dust reply since page banish as regards but facile, quick & eco-friendly microwave assisted adjustment of doublet polymers polymethyl methacrylate & poly butyl methacrylate and joining nanocomposites Ag/Polymethyl methacrylate (Ag/PMMA) then Ag/ Poly butyl methacrylate (Ag/PBMA). The synthesized nanoparticles had been characterized through FT-IR, UV-visible, X-Ray Diffraction (XRD) afterward Transmission Electron Microscopic (TEM) studies. Nanocomposites, Scanning Electron Microscopy (SEM) and TG/DTA.XRD studies but TEM pictures evolved average particle quantity concerning synthesized nanoparticles 20 nm. Thermal lookup rose that standard hot regarding Ag/PMMA then Ag/PBMA nanocomposites reduced as it should be among pursuance with city related to Ag nanoparticles of the polymer matrix.Keywords: Green synthesis, Metal nanoparticles, Metal/ Polymer Nanocomposite, Medical Application
Detection of Fima and Fimh Genes in Klebsiella pneumoniae Isolated from Necrotizing Enterocolitis Patients in Infants Luma Abdalhady Zwain
Journal of Global Pharma Technology Volume 11 Issue 05.
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Sixty-four clinical isolates were collected from the children's protection hospital by Medical city in Baghdad for children infected with Necrotizing Enterocolitis where included (41) samples belong to stool and (14) samples belong to blood and (9) samples belong to urine for the period of 29/1/2018 to 4/4/2018. All the samples were cultured on MacConkey agar and Blood agar for the purpose of diagnosis.  All isolates were identification depending on macroscopic, microscopic, biochemical tests and definite with Vitek-2 compact system, forty three isolates were obtained from all samples, (26) isolates as K. pneumoniae (60.46%) and 8 isolates as E. coli (18.60%) and 4 isolates as P.aeruginosa (9.30%) and 2 isolates were K.oxytoca (4.65%) and 2 isolates were E. cloacae (4.65%) and 1 isolates was P. hauseri (2.32%). The results showed that K. pneumoniae was the predominant in the samples which taken from infants infected to Necrotizing Enterocolitis. In these study, oame of virulence genes have been investigated for K.pneumoniae included both fimA and fimH gens which encodes for the fimbriae adhesion protein that belonging to type 1 fimbriae named Fim protein, and the results showed presence of fimA and fimH genes (100 %).Keywords: Klebsiella pneumoniae, Isolation, Identification, Necrotizing, Enterocoliti, fimA, fimH.
Genotype Distribution Pattern, Subgenotipe and Subtipe of Hepatitis Virus B in Generalpopulation from Six Munipios in Timor-Leste Da Silva H
Journal of Global Pharma Technology .
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Hepatitis B virus infection (HBV) remains a serious health problem in Timor Leste, but data on its prevalence is still very limited. According to some reports, the prevalence of HBsAg in the general population in Timor-Leste ranges from 6.9% - 8.1%. Previous research on blood donors in the city of Dili also indicated the biodiversity of the HBV genotype and HBsAg subtype. This study aims to determine the distribution patterns of genotypes, subgenotypes and subtypes of HBV in the general population in six municipios present in Timor-Leste. This study was conducted using a Cut Lintang design to find out, genotype distribution pattern, subgenotype and HBV subtype. HBsAg examination is done by immunochromatography method. Determination of genotype, subgenotype and HBV subtype was done by analysis of nucleotide sequence of S. gene. The results of 683 subjects from six Munisipio in Timor Leste obtained 69 positive HBsAg. From 69 samples of HBsAg tested positive, genotype C predominant (72,8%), followed by genotype B (25,7%) and genotype A (1,5%). From the subgenotype A1, B3, B7, C1, C2, C7 and C10 there were 3 dominant subgenotypes, subgenotipe C2 (25,8%), followed by subgenotipe C10 (19,7%) and B3 subgenotype (16 , 6). All the major subtypes of HBsAg were found in Timor-Leste, subtype adr (71.2%), followed by ayw subtype (24.2%), adw subtype (3.1%) and ayr subtype (1.5%). Conclusions from this study were three genotypes of HBsAg in the general population of 6 municipios present in Timor-Leste ie genotypes A, B, and C, Subgenotypes A1, B3, B7, C1, C2, C7 and C10, subtype adr, adw, ayw ayr. Genotype C, subgenotype C2, C10 and B3 and subtype adr are the most common genotypes, subgenies and subtypes found in the general population at 6 Municipio in Timor-Leste.Keywords: Genotype, Subgenotype, Hepatitis B Virus Subtype, Timor-Leste.

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