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Journal of Global Pharma Technology
Published by Universitas Udayana
ISSN : 09758542     EISSN : -     DOI : -
Core Subject : Health,
Medicine & Pharmacology
ournal of Global Pharma Technology is a monthly, open access, Peer review journal of Pharmacy published by JGPT Journal publishes peer-reviewed original research papers, case reports and systematic reviews. The journal allows free access to its contents, which is likely to attract more readers and citations to articles published in JGPT. JGPT publishes original research work that contributes significantly to the scientific knowledge in pharmacy and pharmaceutical sciences- Pharmaceutics, Novel Drug Delivery, Pharmaceutical Technology, Cosmeticology, Biopharmaceutics and Pharmacokinetics, Pharmacognosy, Natural Product Research, Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmaceutical Analysis, Pharmacology, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics, Biotechnology and Applied Computer Technology. For this purpose we would like to ask you to contribute your excellent papers in pharmaceutical sciences.
Arjuna Subject : Kedokteran - Onkologi
Articles 81 Documents
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Search results for , issue "Volume 11 Issue 03" : 81 Documents clear
Extraction of Genomic DNA from Formalin Fixed - Paraffin Embedded Tissue for Forensic DNA Analysis: Influence of Proteinase K Maysaa Adil Hadi
Journal of Global Pharma Technology Volume 11 Issue 03
Publisher : Journal of Global Pharma Technology

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Abstract

A huge part of tissues preserved throughout the world used in diagnostic aim is formalin-fixed and paraffin embedded tissue (FFPET) which are a highly worthy supply for retrospective (genetic) application contain screening of mutations in cancer-critical genes in addition to detection of pathogens. The purpose of present study was to estimate the best method in the extraction of DNA from FFPET for utilizing in the forensic analysis by comparing three various extraction methods with and without proteinase K digestion and estimated the influence of these procedures of extraction in DNA quality, quantity and polymerase chain reaction (PCR) amplicon. In this study, the comparison was between three DNA extraction methods from five identically processed FFPET. The extraction methods, including DNA extraction kit, phenol: chloroform: isoamyl alcohol with and without proteinase K methods. Amplifiable DNA fragment size was assessed by amplification of exon 7 of CYP1A1 gene and the size of the fragment was (312 bp). Extractions by different methods used in this study were found suitable for amplification of fragment (312 bp) from all tissues used. The best amplification was after extraction without proteinase K and DNA extraction kit. In conclusion, amplification by PCR shows extremely affected by the method of extraction utilized. Pre-treatment of proteinase K was not a prerequisite for extractions of DNA from FFPET and the suitable extraction method was phenol: chloroform: isoamyl alcohol method without proteinase K as it was less time, less cost, yielding a good DNA quantity and quality.Keywords: Formalin Fixed-Paraffin Embedded Tissue, Forensic Analysis, Proteinase K.
Molecular Detection of Some Virulence Genes of Escherichia coli Isolated from UTI Patients in Kirkuk City, Iraq Ibraheem Salih Aljebory
Journal of Global Pharma Technology Volume 11 Issue 03
Publisher : Journal of Global Pharma Technology

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Abstract

This study was conducted at Azadi teaching hospital, Kirkuk hospital and children hospital in Kirkuk city, Iraq. A 150 urine samples were collected from both genders and different ages of patients with suspected urinary tract infections according to the clinical manifestations and symptoms diagnosed by the examining physician then, these samples have been examined by an optical microscopy and bacterial cell detection which was a clear evidence of the existence of pathogenic bacteria. The positive samples were cultured and were kept for the purpose of detecting genes encoding for certain virulence factors by a polymerase chain reaction (PCR) and also to test the antibiotic sensitivity of these isolates. Out of 150 samples, 93 of them were positive pathogenic bacteria. 51 (34%) of which infected with E. coli. 37(72.5%) out of 51 were from patients suffering from Cystitis and the other 14(27.4%) were from patients suffering from Pyelonephritis. The isolates were identified by selective media culture and biochemical test and API 20 E. Our study showed that females 39 isolates (76.4%) were infected more than males 12 isolate (23.5%), especially in the age group between (19-47 years). The DNA was extracted from Escherichia coli isolates by gene aid DNA isolation kit. Polymerase Chain Reaction was used for detecting the virulence genes (fimH, kpsMTII, papC and hlyA ). The result showed that 51 (100%) isolates were carrying fimH gene, 39 (76.4%) isolates were carrying kpsMTII gene, 23 (45%) isolates were carrying papC gene and 13 (25.4%) isolates were carrying hlyA gene.
Fixed Orthodontic Appliance Associated With Change in Bacterial Diversity During First Stage of Active Orthodonotic Treatment Ahmed S. Al-Janabi
Journal of Global Pharma Technology Volume 11 Issue 03
Publisher : Journal of Global Pharma Technology

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Abstract

Background: some researchers have noted that fixed orthodontic appliance (FOA) have stirring on oral hygiene that lead to high cariogenic challenge. Moreover, based on the difficulty of maintaining oral hygiene, it can also affect germs under the gums by orthodontic devices, These variables would probably lead to the colonization of pathogenic bacteria, which are responsible for inflammation of the gingival, destruction of the periodontal support and changes in the enamel surface. Objective:  Isolation and identification of bacteria among orthodonotic patients at progressive time during first stage of active orthodonotic treatment and molecular identification of highly cariogenic bacteria Streptococcus mutans as well as an in vitro evaluation of antibiotic sensitivity/resistance for bacterial isolates.  Material and Methods: Sixty-five patients treated with fixed orthodontic appliance (FOA) their age between 12-25 years. Imprint swab samples were collected between brackets on the tooth surface monthly at zero day, 1st, 2nd and 3rd to be cultured aerobically and anaerobically. Bacterial isolated were identified in all age groups at progressive time. Molecular detection of S.mutans was performed using species specific primer Sm 479. The antibiotics sensitivity were done by use Kirby- Bauer disc diffusion method for bacterial isolates, the antibiotic selected was most common antibiotic used during orthodontic infection ( amoxicillin, amoxi-clav, cefotaxim, erythromycin and ciprofloxacin). Results:  A total 186 bacterial isolates were obtained from 65 sample of tooth swabs in zero day (immediately after orthodontic device appliance) and 183 bacterial isolates were obtain at first month after wearing orthodontic device, while 195 bacterial isolates were obtained at second month after wearing the device, in the last visit (3rd month after put orthodontic device) 202 bacterial isolates were obtained, and all samples give positive bacterial culture as shows in table (2). Also the result shows that Staphylococcus spp. was the most bacterial isolates appear in patients with fixed orthodontic appliance (FOA). The study indicated neglected statistically significant difference over progressive time except alpha and beta streptococci and P.aurginosa according to Chi-square test. The bacterial isolated during this study include Staphylococcus (S. epidermidis and S.aurus), Lactobacillus spp., Streptococcus (alpha, beta, and gamma), E.coli, Klibsilla spp., Enterobacter spp., and P.aurginosa. Species specific primer Sm 479F/R using polymerase chain reaction (PCR) indicated that S.mutans was detected and increased from 60% at zero day to 80% at 3rd month. The antibiotic sensitivity test recorded that ciprofloxacin and amoxi-clav shows high effective against bacterial isolates. Conclusion: The studies conclude that oral cavity colonized by large number of microorganisms that contribute in infection during orthodontic treatment. Staphylococcus (aurus and epidermidis) was the most common bacteria isolated from patients during initial stage of orthodontic treatment which appear in (81.5%) of the total isolates followed by Lactobacillus spp. and then streptococcus spp. which appear non-statistically significant difference (p ≥0.05) among progress with treatment. The most common gram negative bacteria in zero day is E.coli (20%), Klibsilla (18%), Enterobacter spp.(20.8) while pseudomonas aeriginosa (10.6) and also shows non-statistically difference except P.aurginosa, The flora exists in harmony with the host but this relation may be broken due to orthodontic device. The molecular detection of S.mutans by species specific primer shows increase in percentage from 60% in zero days to 80% in third month. All gram positive showed high level of susceptibility   to amoxi-clav followed by ciprofloxacin and cefotaxime while gram negative show high sensitive to erythromycin followed by ciprofloxacin and cefotaxime.Keywords: Fixed orthodontic appliance, Bacterial diversity, Antobiotic sensitivity, S. mutans.
Synthesis, Spectral and Antibacterial Activity of Mixed Ligand from Lanthanium (III) Complexes of Schiff Base Ligand with Some Amino Acids Shatha M. H. Obaid
Journal of Global Pharma Technology Volume 11 Issue 03
Publisher : Journal of Global Pharma Technology

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Abstract

In this work, lanthanium (III) complexes were synthesized using by Schiff base ligand (L) derived from benzaldehyde and o-aminoaniline with five amino acids (AA) from glycine (Gly), L-alanine (Ala), L-valine (Val), L-asparagine (Asp) and DL- phenylalanine (Phe). The Schiff base ligand has been characterized by elemental analysis, (MASS, FTIR, 1HNMR, 13CNMR, UV-VIS) electronic spectra. The structures of the new complexes have been described of analysis of elements, molar conductivity, (UV-Vis electronic, FT-IR, mass) spectra also magnetic moment. The molar conductivity values of the complexes indicat this every of complexes are electrolytes and other analytical studies reveal octahedral geometry for La (III) ion. The Schiff base ligand, five amino acids, La(III) salt and mixed ligand complexes were tested against four pathogenic bacteria such as G+- bacterial and such as G-- bacterial. These bacteria were found that compounds show, it has no effect on five amino acids and Schiff base ligand, while it has been shown to have a clear effect on lanthanum complexes.Keywords: Schiff base ligand, Amino acids, Mix ligand complexes, Spectral studies and antibacterial studies.
Monosodium Glutamate Effects on Inflammation Status in Male Rats: Molecular and Physiological Study Eman H. Al-Fadhili
Journal of Global Pharma Technology Volume 11 Issue 03
Publisher : Journal of Global Pharma Technology

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Abstract

The current study included the effect of Monosodium Glutamate (MSG) on male rats by measuring the level of  Physiological parameter by determining the level of  total serum  protein, albumin and by measuring the level of  Molecular parameter  by determining the level of IL-6, NF-κb, IKK and TNF-a. The study was conducted at in the Department of Biology, College of Science, University of Wasit from November, 2017 until April, 2018. Study was used nigh teen adult wister rats male weighing 150-250 g and 10-14 weeks age were used and then randomly divided into three group six animal in each group. The first group was control; while the second group was orally dose the MSG 100g/kg dissolved in distilled water and third group take orally dose from MSG 200g/kg dissolved in distilled water via orogastric gavages  for 30 days. The results were as follows:  weight gain significant increase (p ≤ 0.05) of for group that treatment with 100 mg/kg and 200 mg/kg when compare with control group and no a significant (p ≤ 0.05) in concentration of total serum protein, albumin and globulin in male rat that treated with MSG 100 mg /kg . Whereas serum total protein, albumin and globulin  show a significant decreases in rats that treated with  200 mg /kg of MSG  compere with control group, increases of Nf- κB treated with MSG as 100 mg/kg and 200 mg /kg when compere with control group, increases of IL-6 treated with MSG as 100 mg/kg and 200 mg /kg when compere with control group, increases of TNF-a treated with MSG as 100 mg/kg and 200 mg/ kg when compere with control group and decreases of IKK treated with MSG as 100 mg/kg and 200 mg/kg when compere with control group. Conclusion: MSG as food additive can cause weight increase, soluble total protein decrease significant, MSG increases gene expressionKeywords: MSG, Gene expression, Obesity
Study of the Relationship between Some Genetic Markers and Some Productive Traits of Arabi Sheep in the Steppe of Muthanna Hadi Awad Hassooni Al-Brkat
Journal of Global Pharma Technology Volume 11 Issue 03
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Abstract

The study carried out in the first research station to the College of Agriculture-Al-Muthanna University, 12 km south west of Muthanna district and laboratories specializing in molecular genetics for the period from 15/11/2017 until 30/4/2018 in order to determine the relationship between genetic markers BM1258 and INRA023 with genotypes (90/125,120/135,95/140 and 100/90) and (190/220.180/200 and 185/170) bp Respectively and the production of milk and Its components the following are the most important results obtained: The presence outweigh the significant (P≤0.05) for marker BM1258 in ewes carrying genetic site 90/125 bp in the total production of milk and the length of the milking season the ewes bearing on others local 120/135 , 95/140 and,100/90 bp .while outperformed significantly (P≤0.05) ewes 120/135 bp genetic location in the proportion of fat significantly milk ewes bearing two hereditary 90/125 and 95/140 bp, which varied among them. The presence outweigh the significant (P≤0.05) for marker INRA023 in ewes bearing site genetic 185/170 in the total production of milk and the length of milking the ewes carrying two 190/220 and 180/200 season, while the superiority of ewes same location genotype 180/200 in the percentage of milk fat. Affected the results and there is a significant difference (P≤0.05) for Marker INRA023 in ewes same location genotype 180/200 bp in the proportion fat solids and protein content.Keywords: Sheep, Genetic markers, BM1258, INRA023.
The Effect of Calcium Oxide Nanoparticles on Liver Enzymes in White Rats Shurooq Wesam Al-Shaibani
Journal of Global Pharma Technology Volume 11 Issue 03
Publisher : Journal of Global Pharma Technology

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Abstract

Calcium is the five elements that found on the crust of the earth. It`s the essential mineral for all organisms because the important role in the functions of the life cells , and its essential mineral for binding the bone and teeth by producing calcium phosphate. The decreasing of calcium element in the body leads to weakness, dental caries, and muscle cramps. Present study was designed to evaluate the effect of calcium oxide- nanoparticles on the serum levels for liver enzymes of white rats. Two group of White rats ( 3 rats each group) received calcium oxide- nanoparticles which were orally administered with 50 and 100 (mg/kg) from of body weight of calcium oxide- nanoparticles suspension daily for 10 day. Serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) were measured at start and at the end of the study.  Changes of the specific parameters indicated that liver were significantly affected in both experimental groups , the changes between the levels of total alanine aminotransferase , aspartate aminotransferase and alkaline phosphatase indicate that calcium oxide- nanoparticles induces liver damage and may be lead to hepatic toxicity in the experimental rats.Key words: CaO-Nanoparticles, (ALT, AST and ALP), White rats.
Synthesis and Characterization of [1-phenyl-3-naphthoyl-2-thiourea] and Its Metal Complexes with [Co (II), Ni (II), Cu (II), Zn (II), and Cd (II)] ions and Study Antibacterial Activity and Corrosion Inhibitor Basim Ibrahim Al-Abdaly
Journal of Global Pharma Technology Volume 11 Issue 03
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Abstract

The formation of Co (II), Ni (II), Cu (II), Zn (II) and Cd (II)-complexes (C1-C5) respectively were studies with new thiourea ligand [1-phenyl-3-naphthoyl-2-thiourea] from 2-naphthoic acid in three steps. The suggested structures of the ligand and its complexes have been determined   by using C.H.N.S analyzer, thermal analysis, FT-IR, U.V-Visible, 1HNMR, 13CNMR, conductivity measurement, magnetic susceptibility, and atomic absorption. According to these studies, the ligand coordinates as  a bidentate with metal ions through  oxygen atom of  carbonyl, and  sulfur atom of thione  and the ratio of  metal to ligand (M:L) as (1:2) and all the complexes were octahedral structures except copper-complex was distorted octahedral structureKeywords: Thiourea ligand, antibacterial activity, Corrosion inhibitor.
Measurement of Pollution with Heavy Metals in Water and Sediment Dalmage Lake-Middle of Iraq Esraa A. Elewi
Journal of Global Pharma Technology Volume 11 Issue 03
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Abstract

The current study aims at determining the quality of the water and the sediments of the Dalmage Lake in terms of pollution. Where some physical characteristics and concentrations of some heavy elements were measured by three different stations from Dalmage Lake which is located in middle of Iraq between Qadisiyah and Al-Kut provinces, from September 2017 to January 2018 . The studied properties included temperature, pH, electrical conductivity and measurement of concentrations of heavy metals (cadmium, lead, copper, cobalt and iron) in the water and sediments of the Dalmage Lake. The results of the study showed that the temperature of the air is compatible with the temperature of water in all stations of the study where the temperature of air between (18 - 35.3)0c and water temperature (12-25)0c. The electric conductivity was recorded between (9.89 - 16.81) ms/cm and the pH values ranged from (7, 72 - 8, 16). As for the concentrations of heavy elements in the water of Dalmage Lake, the study showed that the cadmium concentration ranged between (0.034 - 0.082), lead (0,041 - 0,753), copper (0.039-0,354) Cobalt (0.050 - 0.189) and iron (0.405 - 0.490) mg / L. While in the sediment of Dalmage Lake the pH values (7, 41 - 8, 09) were the electrical conductivity (12.97 - 17.13) ms/cm and the concentrations of heavy metals were recorded for cadmium (0,093-0,010), lead (1,202 - 1,708), copper (0,180 -), Cobalt (0.157-0.521) and Iron (35,170 - 115,971) mg/L. The study showed that the water of Dalmage Lake has a basic character. This corresponds to the Iraqi water which is characterized by basal quality. The electrical conductivity is high because the water of the lake is sality, and the study showed an increase in concentration of heavy elements, this means that the lake is polluted by its because of incompatibility with values of WHO and local standards .Keywords: Pollution, Dalmage Lake, Heavy Metals, Sediment.
Using Tobacco Leaves as Adsorbent for the Orange-G Dye Removal from its Aqueous Solutions Rawaa Abbas Mohammed
Journal of Global Pharma Technology Volume 11 Issue 03
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Abstract

The removal of commercial orange G dye from its aqueous solution by adsorption on tobacco leaves (TL) was studied in respect to different factor that affected the adsorption process. These factors including the tobacco leaves does, period of orange G adsorption, pH, and initial orange G dye concentration .Different types of isotherm models were used to describe the orange G dye adsorption onto the tobacco leaves. The experimental results were compared using Langmuir, and frundlich adsorption isotherm, the constants for these two isotherm models was determined. The results fitted frundlich model with value of correlation coefficient equal to (0.981). The capacity of adsorption for the orange G dye was carried out using various kinetic models like pseudo first order-kinetic, pseudo second order –kinetic, Elovich, and inter particle diffusion model, the rate constants for these models were evaluated. The data suggested that tobacco leaves are suitable sorbent for the orange - G dye removal from its solution.Keywords: Adsorption, Orange G, Tobacco leaves.

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