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Journal of Global Pharma Technology
Published by Universitas Udayana
ISSN : 09758542     EISSN : -     DOI : -
Core Subject : Health,
Medicine & Pharmacology
ournal of Global Pharma Technology is a monthly, open access, Peer review journal of Pharmacy published by JGPT Journal publishes peer-reviewed original research papers, case reports and systematic reviews. The journal allows free access to its contents, which is likely to attract more readers and citations to articles published in JGPT. JGPT publishes original research work that contributes significantly to the scientific knowledge in pharmacy and pharmaceutical sciences- Pharmaceutics, Novel Drug Delivery, Pharmaceutical Technology, Cosmeticology, Biopharmaceutics and Pharmacokinetics, Pharmacognosy, Natural Product Research, Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmaceutical Analysis, Pharmacology, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics, Biotechnology and Applied Computer Technology. For this purpose we would like to ask you to contribute your excellent papers in pharmaceutical sciences.
Arjuna Subject : Kedokteran - Onkologi
Articles 93 Documents
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Search results for , issue "Volume 11 Issue 05." : 93 Documents clear
Various Technical Aspects in Herbal Tea Production from Alisma Plantago-Aquatica Leaf N. P. Minh
Journal of Global Pharma Technology Volume 11 Issue 05.
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Abstract

Alisma plantago-aquatica is abundant weed on thinned crops of rice. A. plantago-aquatica is resistant to flood, but being very susceptible to shade; therefore the thickened rice crops shade the weeds and detain their growth. Leaves green; lower ones only sessile, widely linear, and swimming; the other ones aerial, long-petiolate; their plates big, ovoid or widely ovoid, with cordate base usually, with pointed apex. The use of medicinal herbs as a source of antioxidants is significant because they are often utilized to heal chronic diseases. Objective of this study focused on the effectiveness of blanching temperature and time; oven drying temperature and storage condition to total phenolics (mg/g), total flavonoids (mg/g), ferric reducing/antioxidant power (FRAP, mg/g), radical-scavenging activity (DPPH, mg/mL) of the dried Alisma plantago-aquatica tea. Results showed that Alisma plantago-aquatica should be treated in hot water at 95oC within 8 seconds in the present of citric acid 1.0% and then being dried by drying oven at 45oC until 7.5% moisture. The final herbal tea could be preserved under vacuum in aluminum bag at 28oC to maintain total phenolics, total flavonoids, antioxidant power and radical-scavenging activity for 12 months.Keywords: Alisma plantago-aquatica, Phenolic, Flavonoid, Herbal, FRAP, DPPH, Blanching, Drying.
Molecular Detection of Some Virulence Genes of Salmonella Typhymurium and Salmonella Enteritidis Isolated from Patients Suffering Watery Diarrhea Lamees A. Abdul-Lateef
Journal of Global Pharma Technology Volume 11 Issue 05.
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Abstract

Salmonella enterica virulence factors can be divided into chromosomal, plasmid and bacteriophages encoded virulence factors. Many of the Salmonella virulence factors, such as adhesion, invasion, and toxin genes are clustered in certain areas of the chromosome known as “Salmonella pathogenicity islands” (SPI). A total of 200 clinical stool samples were collected during this study which obtained from patient Suffering from watery diarrhea who a dmintted to three main hospitals of Babylon Governorate: Merjan Medical city, Al-Hillah Surgical Teaching Hospital and Babil Teaching Hospital for Women and Children during a period of three months (from july 2018 to September 2018). Out of 200 specimens 35(17.5%) were detected by culture and vitek2 compact, 24 (12%) of them were confirmed by PCR using target gene, (17) Salmonella typhimurium and (7) Salmonella enteritidis. PCR was conducted to determine the some virulence genes of the isolates by using primers inv A, sdiA and sifA. The PCR amplification products were visualized by electrophoresis on 1% agarose gels for 35min at 70v. The sizes of the amplicons were determined by comparison to the 200 bp allelic ladder. Among isolates studies it was found that invA gene present in 13(54)% isolates of S. enterica ,(9) isolates of S. typhimurium and (4) isolates of S. enteritidis , sdiA gene it was found in all isolates (100)% while sifA gene it was found in all isolates only isolate number 18 not contained this gene 23(96)%.Keywords: Salmonella typhimurium, S. enteritidis, invA, sdiA and sifA.
The Role of Mobile Phones in the Transmission of Methicillin-Resistant Staphylococcus aureus (MRSA) among the Students and Staff of the College of Health and Medical Technology/ Kufa in Najaf, Iraq Oday Mitib Hadi
Journal of Global Pharma Technology Volume 11 Issue 05.
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Abstract

Background: During every phone call the mobile phone, come into close contact with strongly contaminated human body areas with hands to hands, and hands to other areas like mouth, nose, and ears [10]. Mobile phones touch faces, ears, lips, and hands of different users thus they may be serves as reservoirs of different pathogens [11].  This study was designed to determine the role of the mobile phones in the transmission of methicillin-resistance Staph. aureus. Methodology: The cross-sectional study was done in the college of health and medical technology/ Kufa- Najaf- Iraq. Three hundred swab samples from mobile phones were collected from the students and staff of the college from November- 2015- February-2016 and divided into three groups categorized according to the site of collection (from the mobile phones) as follows: earpiece, keypad or touch screen, and mouthpiece. All samples were cultured, and the resulting isolates were identified and subjected to antimicrobial susceptibility test by standard procedures (Kirby-Bauer method).Methicillin-resistant Staph. aureus was detected by using polymerase chain reaction (PCR) based on mecA gene.   Results: Keypad or touch screen was occupied the highest percentage (58.74%) of bacterial contamination among other regions i. e. earpiece (26.7%) and mouthpiece (14.83%).Coagulase-negative Staph. aureus was predominant (41.83%) bacterial genus among other Gram-positive genera. Other Gram-positive bacteria had different ratios; there were 28.37%, 19.71%, 8.65%, 0.96% and 0.048 for Micrococcus spp., Bacillus spp. Methicillin-sensitive Staph. aureus (MSSA), Methicillin-resistant Staph. aureus (MRSA), and Enterococcus faecalis, respectively. On the other hand, Gram-negative bacteria gave different percentages, ranging from 53.57% for E. coli to 3.57% for Salmonella spp. Molecular detection was revealed the presence of mecA gene in only two isolates of Staph. aureus. Conclusion: Mobile phones may serve as vehicles of transmission of methicillin-resistance Staph. aureus.Keywords: Mobile phones, Methicillin Resistant Staph. aureus,  Meca gene.
Application of H-Point Standard Addition Method in Kinetic-Spectrophotometric Determination of Phenylephrine in Nasal Drops and Tetracycline in Capsule Faeza H. Zankanah
Journal of Global Pharma Technology Volume 11 Issue 05.
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Abstract

A new simultaneous spectrophotometric-kinetic method was developed to determine phenylephrine (PHEN) and tetracycline (TETR) via H-point standard addition method (HPSAM). The proposed procedures rely on the measurements of the difference in the rate of charge-transfer (CT) reaction between each of PHEN and TETR as electron donors with p-Bromanil (p-Br) as an electron acceptor. Different experimental factors which affect the extent of the complex formation were investigated by monitoring the value of absorbance at 446 nm. Time pair of 50 -100 sec was selected and employed, among different examined pairs since it results in the highest accuracy for HPSAM-plot. Linear calibration graphs in the concentration ranges of 10.0-40.0 and 10.0–50.0 mg/L were obtained for PHEN and TETR, respectively and the proposed method was applied successfully in the determination of PHEN and TETR in their pure form and in commercial formulations.Keywords: Spectrophotometry, Kinetic H-point standard addition method, Phenylephrine, Tetracycline, Simultaneous analysis.
Radon Concentration Measurements and Physiochemical Parameters of Sawa Lake Water-Samawa City, South of Iraq Ahmed Augab Sharrad
Journal of Global Pharma Technology Volume 11 Issue 05.
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Abstract

Radon concentration measurements were carried out for 85 water samples collected from Sawa Lake in Samawa city, Southern part of Iraq, in order to obtain the levels of radon concentration in this area and for enhancing the national radon survey. For the measurements, a continuous radon monitoring detector (RAD7) was used. The period of assessment was two months (from December, 1st  2018 to February, 1st  2019) during the winter season. Radon activity concentrations were ranged from (0.111 Bq/l) to (0.965 Bq/l), while the average value was (0.396 Bq/l), the annual effective dose (AED) of inhalation was estimated from the measured radon concentrations and is found to be ranged from (0.080784 µSvy-1) to (0.917136 µSvy-1) with a mean value of (0.325764 µSv/y). Physiochemical parameters such as pH, EC, TDS, resistivity, and turbidity were determined samples and the correlation between these parameters and radon concentrations was conducted. The results have revealed that the radon concentration and the associated annual effective dose does not pose any kind of health hazard to the population and tourists, and were found to be well within the reference levels as reported in the ICRP 2000.Keywords: Radon, Sawa Lake, Samawa City, Physiochemical Parameters, RAD7Detector.
Molecular Detection of Lysinibacillus Fusiformis Isolated from Milk Samples of Cow in Iraq Aseel Mohammed Hamzah
Journal of Global Pharma Technology Volume 11 Issue 05.
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Abstract

Raw milk  infected by bacteria can start from exceptional resources: air, milking devices, feed, soil, dangs and hays.The strategies of  nourishing and lodging cows may impact the germs pleasant of milk. Bacterial contaminants can cause infection, or deterioration of drain and its auxiliary items and the destructive impacts on the grade and security of dairy items as a result of oxygen consuming spore-forming bacteria got from crude drain were characterized by isolation of Lysinibacillus fusiformis from 90 milk samples of cow at 21.6l%, the bacteria were identified by routein bacteriological methods and Molecular distinguishing proof of the confines was carried out by 16S rRNA sequencing and the bacterial confines were taxonomically classified as Lysinibacillus fusiformis. The groupings were stored in NCBI GenBank with the accession number KY038703, KF916675. and KF916675.1 with identity of 100%, 96 and 99% respectively. Keywords: Raw milk, Lysinibacillus fusiformis, 16S rRNA PCR sequencing.
Synthesis and Characterization of Some New Quinolinic acid Derivatives Haneen Nazar Wahab Al-Issawi
Journal of Global Pharma Technology Volume 11 Issue 05.
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Abstract

Preparation of some non-homogeneous cyclic compounds starting with Quinolic acid .A different N-heterocyclic substituted derivative of quinolone has been synthesized. The nitrogen atoms have been alkylated first to form N-carboxymethyl followed by cyclization reaction to form heterocyclic/substituted aryl groups. All the synthesized compounds have been identified using FT-IR, 1H NMR spectrum.Keywords: Quinolinic acid, 1, 3-Oxazole, Imidazol, 1, 2, 4-Triazole, 1, 3, 4- thiadiazol, diazetidin, diazepines.
Synthesis and Characterization of New (2-(((5-(3, 5-Dinitrophenyl)-1, 3, 4-Thiadiazol-2-yl-2,5 Dihydroxy Benzalidine))) Metal Complexes Ali T. Bader
Journal of Global Pharma Technology Volume 11 Issue 05.
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New  complexes  compounds of some metal ions such as [VO(IV)), Co(II) Ni(II),Cu(II),Zn(II) and Cd(II)] are synthesized with  ( 2-(((5-(3, 5-dinitrophenyl)-1, 3, 4-thiadiazol-2-yl-2,5dihydroxy benzalidine. The ligand Schiff base was synthesized by condensation of 2-amino -5-(3,5dinitro phenyl )1,3,4-thiadiazole with 2,5dihydroxy benzaldehyde .The suggested structures of new complexes are characterized by elemental micro analysis (C.H.N), FT-IR, UV-Vis spectra, Thermal gravimetric analysis (TGA-DTA), flame atomic absorption, molar conductivity, magnetic susceptibility and Mass Spectra.  The suggested theses complexes according to the results data the probable coordination geometries are octahedral complexes   excepted C1 was pyramidal. The all complexes were found to be non-electrolyte.Keywords: 1, 3, 4-thiadiazole, Schiff base and metal ions
Genotyping of Human Cytomegalo Virus Envelop Glycoprotein B in Iraqi Immunocopromised and immunosupresser Patients Raghad Harbi Mahdi Al-Azzawi
Journal of Global Pharma Technology Volume 11 Issue 05.
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Envelope glycoprotein B is a surface protein of CMV which plays an important role in the pathogenesis of the virus, polymorphism in the gB gene may interfere with transmission of infection. This study was aimed to investigate the HCMV glycoprotein B genotypes in immunosupressed and immunocompromised Iraqi patients. A total of 362 Iraqi patients were collected from immunosupressed (pregnant women and infants) and immunocompromised (renal transplant and malignancies patients) that have been tested for the presence of acute infection of HCMV, during the period from November 2014 to February 2015. While another 20 individuals with negative serum IgM/IgG were included as negative control. The blood samples were positive for CMV-IgM and both IgM /IgG by ELISA and ELFA were found to be present in 85 (23.48 %) out of 362 blood samples. Five PCR confirmed isolates were chosen randomly for DNA sequences and similarity. Searches were carried out with the Basic Local Alignment Search Tool (BLAST) in National Center of Biotechnology Information (NCBI). The data showed that the glycoprotein B type 2 was the most common genotypes among the studied immunocoprimised and immunosupressed Iraqi patients. The nucleotide sequences of the glycoprotein B UL55 region from HCMV in this study have been deposited in the Gen Bank sequence database of HCMV under the following accession numbers: M17209.1, KP745721.1 and X17403.1.
Effect of Cutting Frequency and Potassium Levels on Seed Yield and its Components for Berseem (Trifolium alexandrium L.) Mahmood Abbas Abd Salama
Journal of Global Pharma Technology Volume 11 Issue 05.
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A study was conducted at one locations in the AL-Jazeera region at north of Ramadi city for winter season (2017) to investigate the affect number of cutting and  different levels of potassium on seed yield and  its components in berseem. Four cutting treatments (once ,twice, three and four times ) with potassium levels included  (0,80,160 and 240 kg/he.-1).Were applied in a split plot design with three replication in which potassium levels were the main plots and cutting treatments were sub-plots . The results showed that significant effect on seed yield and its components, added potassium at levels (160) kg/ha produced significant higher means for all components higher seed yield (38.96 no. of seeds/flower, 2.90g. and 2.06 ton/ha.-1) was obtained from treatments received (160)kg/ha.-1 of potassium . Number of cutting stage had significant effect on seed yield and all components in winter season plants cut once gave higher seed yield (1.74 ton/ha.-1 )the 1st cut showed be don in 1st week of march  . It was concluded that addition of potassium at a rate of (160) kg/ha.-1 and cut the crop four times when the 1st cut showed be done in 1st week of March gave higher seed yield of berseem.Keywords: Berseem, Cutting treatments, Potassium levels, Head length, Grain yield. 

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