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Journal of Global Pharma Technology
Published by Universitas Udayana
ISSN : 09758542     EISSN : -     DOI : -
Core Subject : Health,
Medicine & Pharmacology
ournal of Global Pharma Technology is a monthly, open access, Peer review journal of Pharmacy published by JGPT Journal publishes peer-reviewed original research papers, case reports and systematic reviews. The journal allows free access to its contents, which is likely to attract more readers and citations to articles published in JGPT. JGPT publishes original research work that contributes significantly to the scientific knowledge in pharmacy and pharmaceutical sciences- Pharmaceutics, Novel Drug Delivery, Pharmaceutical Technology, Cosmeticology, Biopharmaceutics and Pharmacokinetics, Pharmacognosy, Natural Product Research, Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmaceutical Analysis, Pharmacology, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics, Biotechnology and Applied Computer Technology. For this purpose we would like to ask you to contribute your excellent papers in pharmaceutical sciences.
Arjuna Subject : Kedokteran - Onkologi
Articles 4 Documents
 1 
Search results for , issue "Volume 16 Issue 03 (2024) March 2024" : 4 Documents clear
REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION-A REVIEW Sri, K. Bhavya; Banu, Shaheen; Sumakanth, Mogili
Journal of Global Pharma Technology Volume 16 Issue 03 (2024) March 2024
Publisher : Journal of Global Pharma Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The reverse transcription polymerase chain response is straightforward, powerful, and exquisite innovation in the discipline of molecular biology. It helps in determining the gene expression and amplifying the gene of interest in numerous fields used to locate and quantify the quantity of a given DNA collection. It entails three steps: reverse transcription, PCR amplification, and electrophoresis. Among PCR, the maximum commonly used PCR technique is reverse transcription polymerase chain reaction abbreviated as RT-PCR. it is way utilized in molecular biology & genetic research that allows the detection & quantification of mRNA. in this, RNA template is first converted into a complementary DNA by use of a reverse transcriptase enzyme and is further used for exponential amplification the use of the PCR method. Many genes are recognized to have their own promoter regions that inform cells where to begin transcribing the unique gene. RT-PCR first starts by using the usage of reverse transcriptase enzyme to make DNA out of RNA that has been extracted from cells or tissue samples. The RNA is in the shape of a polymer. The opposite transcriptase copies the RNA into an open-ended DNA strand, preventing it when it encounters a unique non-coding location known as a "poly-A tail" for each 20 to 23 nucleotides. The PCR method then begins by blending the newly made DNA with known primers to expand or reflect a selected piece of DNA. The pattern is then heated and cooled more than one instance, which causes the DNA to split into exclusive string sizes, brief and long. the quick string represents the newly copied RNA that can be visible as amplification, while the longer strand represents each the amplified RNA and all other non-amplified DNA portions collectively. The difference in the duration of DNA strands is what offers reverse transcriptase PCR its name. RT-PCR approach is performed in a thermocycler for the huge production of genetic fabric. It includes several wells, thermos regulators, a detector, and a gadget hooked up with appropriate software. Keywords: Amplification, Thermocycler, Gene expression, cDNA, Molecular biology, RT-PCR, Template.
EXPLORING POST-COMPRESSION PARAMETERS IN MARKETED VILDAGLIPTIN TABLETS Siva, P Krishna
Journal of Global Pharma Technology Volume 16 Issue 03 (2024) March 2024
Publisher : Journal of Global Pharma Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

This study investigates the post-compression parameters of tablets produced by five prominent pharmaceutical brands. Tablets play a crucial role in drug delivery, and their quality is essential for ensuring therapeutic efficacy. The post-compression phase is a critical step in tablet manufacturing, influencing key parameters such as weight variation, hardness, friability, and disintegration. This research aims to assess and compare these parameters for tablets from five selected brands, confirming their adherence to quality standards. The tablets from Brand A, Brand B, Brand C, Brand D, and Brand E underwent a rigorous testing protocol to evaluate hardness using a calibrated hardness tester, friability through Roche friabilator, and disintegration employing a disintegration tester. The study utilized standardized testing methods to ensure consistency and reliability across all assessments. Results revealed that all five brands successfully met benchmarks for weight variation, hardness, friability, and disintegration time. The tablets demonstrated robust mechanical strength, minimal friability, and prompt disintegration of active pharmaceutical ingredients. Keywords: Post-compression, Hardness, Friability, Disintegration.
EXPLORING POST-COMPRESSION PARAMETERS IN MARKETED VILDAGLIPTIN TABLETS Siva, P Krishna
Journal of Global Pharma Technology Volume 16 Issue 03 (2024) March 2024
Publisher : Journal of Global Pharma Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

This study investigates the post-compression parameters of tablets produced by five prominent pharmaceutical brands. Tablets play a crucial role in drug delivery, and their quality is essential for ensuring therapeutic efficacy. The post-compression phase is a critical step in tablet manufacturing, influencing key parameters such as weight variation, hardness, friability, and disintegration. This research aims to assess and compare these parameters for tablets from five selected brands, confirming their adherence to quality standards. The tablets from Brand A, Brand B, Brand C, Brand D, and Brand E underwent a rigorous testing protocol to evaluate hardness using a calibrated hardness tester, friability through Roche friabilator, and disintegration employing a disintegration tester. The study utilized standardized testing methods to ensure consistency and reliability across all assessments. Results revealed that all five brands successfully met benchmarks for weight variation, hardness, friability, and disintegration time. The tablets demonstrated robust mechanical strength, minimal friability, and prompt disintegration of active pharmaceutical ingredients. Keywords: Post-compression, Hardness, Friability, Disintegration.
REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION-A REVIEW Sri, K. Bhavya; Banu, Shaheen; Sumakanth, Mogili
Journal of Global Pharma Technology Volume 16 Issue 03 (2024) March 2024
Publisher : Journal of Global Pharma Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The reverse transcription polymerase chain response is straightforward, powerful, and exquisite innovation in the discipline of molecular biology. It helps in determining the gene expression and amplifying the gene of interest in numerous fields used to locate and quantify the quantity of a given DNA collection. It entails three steps: reverse transcription, PCR amplification, and electrophoresis. Among PCR, the maximum commonly used PCR technique is reverse transcription polymerase chain reaction abbreviated as RT-PCR. it is way utilized in molecular biology & genetic research that allows the detection & quantification of mRNA. in this, RNA template is first converted into a complementary DNA by use of a reverse transcriptase enzyme and is further used for exponential amplification the use of the PCR method. Many genes are recognized to have their own promoter regions that inform cells where to begin transcribing the unique gene. RT-PCR first starts by using the usage of reverse transcriptase enzyme to make DNA out of RNA that has been extracted from cells or tissue samples. The RNA is in the shape of a polymer. The opposite transcriptase copies the RNA into an open-ended DNA strand, preventing it when it encounters a unique non-coding location known as a "poly-A tail" for each 20 to 23 nucleotides. The PCR method then begins by blending the newly made DNA with known primers to expand or reflect a selected piece of DNA. The pattern is then heated and cooled more than one instance, which causes the DNA to split into exclusive string sizes, brief and long. the quick string represents the newly copied RNA that can be visible as amplification, while the longer strand represents each the amplified RNA and all other non-amplified DNA portions collectively. The difference in the duration of DNA strands is what offers reverse transcriptase PCR its name. RT-PCR approach is performed in a thermocycler for the huge production of genetic fabric. It includes several wells, thermos regulators, a detector, and a gadget hooked up with appropriate software. Keywords: Amplification, Thermocycler, Gene expression, cDNA, Molecular biology, RT-PCR, Template.

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