cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
-
Contact Email
-
Phone
-
Journal Mail Official
-
Editorial Address
-
Location
Kab. sleman,
Daerah istimewa yogyakarta
INDONESIA
Jurnal Sain Veteriner
Published by Universitas Gadjah Mada
ISSN : 012660421     EISSN : 24073733     DOI : -
Core Subject : Health,
Veterinary
Arjuna Subject : -
Articles 7 Documents
 1 
Search results for , issue "Vol 16, No 2 (1999)" : 7 Documents clear
HISTOLOGIC PICTURE OF BEEF DIGESTED BY PAYA PAPAIN ENZYME Yatri Drastini
Jurnal Sain Veteriner Vol 16, No 2 (1999)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8595

Abstract

The study was conducted to show the histologic picture of beed digested by different concentration of PAYA papain enzyme. Twenty pieces of beed of m. femoralis, 1 x 0.5 x 0.5 cm in size, were allocated into four groups. Group I was injected with aquadest as control; group II, III, dan IV were injected with PAYA 0.25%, 0.50%, and 0.75% of the weight of meat, respectively. The samples were heated in a waterbath 65°C to activate the enzyme for 0, 15, 30, 45, and 60 minutes. Samples were then put into 10% formalin solution to stop the enzyme. The were stained with haematoxyline-eosine (HE) for histologic picture. The result shows PAYA papain enzyme starts to disintegrate myofibril in the con­centration of 0.50% for 30 minutes. The higher is the con­centration, the more do changes of myofibril take place in the form of inflamation, disintegration, and lysis.
THE EFFECT OF INCUBATION TIME ON EXCYSTATION OF EIMERIA TENELLA SPOROZOITES IN VITRO Joko Prastowo; Mufasirin Mufasirin; Sumartono Sumartono
Jurnal Sain Veteriner Vol 16, No 2 (1999)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8596

Abstract

This purpose of the research is to know the effect of incubation time on excystation of Eimeria tenella sporo-zoites in vitro.Eimeria tenella oocystes treated in clorox were broken by glass beads. Sporocystes liberated from oocys were divid­ed in six groups (I - VI), each ghroup contained 1.68.106 sporocystes. All of the groups were treated by 0,75% taur-ocholat sodium and 0,25% trypsin. Those sporocystes were incubated at 41°C for 15, 30, 45, 60, 75 and 90 minutes respectively. The data collected was the percentage at the number of sporozoites found in each group.The result showed that the increase of the number of sporozoites was paralelly with the incubation time. The highest number of sporozoites excystation was found in the 90 minutes of incubation group.
THE EFFECT OF STORAGE LENGTH ON THE PATHOGENICITY OF EIMERIA TENELLA OOCYSTS Eryl Sri Rohayati
Jurnal Sain Veteriner Vol 16, No 2 (1999)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8598

Abstract

A research has been conducted to evaluate storage length effect on the pathogeniclty of E. tenella oocysts in broiler chickens.Two groups consist eight day old chickens, four chickens in group I and four chichkens in group II were used in this research. At the age of seventeen days, all the chickens in both groups were infected with 20,000 E. tenella oocysts. Group I was infected with Eimeria tenella oocyst that had been stored for 107 days at 4°C in the refrigerator and group II was infected with E. tenella oocysts that had been stored for two days at room temperature.The data collected were the caeca lesion score and the histopathology of the caeca. Of all chichkens in both groups that were killed on the fifth days after infection.The data of the caeca lesion score was analysed using the Rank test (Ferguson and Takane, 1989) while the data of caeca histopathology was analyzed descriptively.The result of the research on the caeca lesion score showed that there was a significant difference between group I and group II (P < 0,01). In group I the caeca lesion score was relative light (0) - (+1) if compared with group two (+4). The result of the research on the caeca histopathology showed that in group I the histopathological changes were relative light if compared with group II. In group II there was a great inflamation reaction and the coccidia stadia varied greatly.The conclusion of the research was when oocysts storage for 107 days will be influence the pathogenicity of the E. tenel1 a oocyst s.
DETECTION PROTECTIVE ANTIGEN WITH MONOCLONAL ANTIBODY OF PASTEURELLA MULTOCIDA DOMPU ISOLATE Sri Murwani; Widya Asmara
Jurnal Sain Veteriner Vol 16, No 2 (1999)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8599

Abstract

Monoclonal antibodies can be used as specific probes for molecular structure such as drugs, hormones, receptor for hormones, or any other biologycally derived or biology-cally active materials. The potential usefulness of mono­clonal antibodies is in almost every field of biology and medicine.The study was carried out to produced monoclonal anti­bodies against outer membrane protein (OMP) Pasteurella multocida isolat Dompu, which can be used to isolate imunogenic OMP fractions.Three monoclonal antibodies which specifically react with OMP fraction of Pasteurella multocida isolat Dompu were obtained previously. Those monoclonal antibodies were pro­duced in Balb/c mouse. The antibodies production were measured by ELISA and were purified by affinity chromatograPhy.The monoclonal antibody were injected intraperitoneally into group of five rats. Two additional groups of five rats were injected with immune mouse serum and normal mouse serum, which served as a positive and negative controls respectively. All rats were challenged with 1 ml lethal dose of Pasteurella multocida 24 hours after the antibody injec­tion.The outer membrane protein was separated 12,5% SDS-PAGE. The region of gel containing the appropiate antigens (PI, P2, P3) were excised. Protein antigen was mixed with incomplete Freund's adjuvant (IFA) and injected intramuscu-lary into group of five rats at dose 0,2 ml per mouse. Two additional groups were injected with gel in IFA and OMP and IFA as a positive and negative controls. Each treated mouse and control was immunized four times, at weekly interval. Two weeks after final immunization rat were bled and their antibodies productions were measured with ELISA. All mice were then challenged with lethal dose of Pasteurella multo­cida.The result indicated that eventhough immungenic the PI, P2, P3 OMP fraction individually were not able to induce protective antibody. 
IDENTIFICATION OF SERPTICAEMIA EPIZOOTICA (SE) ON SWAMP BUFFALOES (KALANG BUFALLOES) IN SOUTH KALIMANTAN Bambang Nganji Utomo; Tarmudji Tarmudji; A. Hamdan
Jurnal Sain Veteriner Vol 16, No 2 (1999)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8601

Abstract

Identification of Septicaemia Epizootica (SE) was carried out on swamp buffaloes at the districts of Hulu Sungai Tengah (HST) and Hulu Sungai Utara (HSU) and those which were slaughtered in the abbatoars of Banjarmasin, Banjarbaru and Amuntai, South Kalimantan. This study was conducted to find out whether Septicaemia Epizootica was present among the swamp buffaloes population. Thirty two samples of swab nasopharynx from the field (HSU and HST) and 60 samples from the abbatoars were collected and checked toward Pasteurella multocida. And 45 samples of sera were examined for antibody with Elisa method. Based on bacterio­logical examination there was no Pasteurella multocida could be identified from the samples and from Elisa examination was also negative from Septicaemia Epizootica.
ISOLATION, IDENTIFICATION AND SENSITIVITY TEST OF BACTERIA FROM DIARRHEA CASES IN DOG AT THE VETERINARY CLINIC, FACULTY OF VETERINARY MEDICINE, GADJAH MADA UNIVERSITY Yuriadi Yuriadi; Ida Tjahajati
Jurnal Sain Veteriner Vol 16, No 2 (1999)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8602

Abstract

A study was conducted to find out bacteria which probably play an important role in causing diarrhea in dog at the veterinary clinic Gadjah Mada University. Sample were collected from diarrhea cases of dog, during the periode of September to December 1997, by rectal swabbing. Samples were then sent to the laboratory for bacterial isolation and identification.The result indicated that E. coli was found from 50% of the sample, Streptococcus sp 41.9%, Staphylococcus sp 33.3% of sample, Enterobacter sp 16.7%, Bacillus sp 8.3%.Some of E. coli isolates were pathogenic to mice. The pathogenic E. coli were resistant to penicillin, ampicillin, streptomycin, erythromycin, doxycycline, besides sensitive to neomycin, gentamycin, kanamycin, polymyxin B, chloram-phenicol and sulfisoksale.
The Susceptibility Of Burkholderia Pseudomallei Strains To Various Chemotherapeutics : a review Jeanne Indriana
Jurnal Sain Veteriner Vol 16, No 2 (1999)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8604

Abstract

...

Page 1 of 1 | Total Record : 7

 1 

Filter by Year

1999 1999


Reset
Filter By Issues
All Issue Vol 43, No 2 (2025): Agustus Vol 43, No 1 (2025): April Vol 42, No 3 (2024): Desember Vol 42, No 2 (2024): Agustus Vol 42, No 1 (2024): April Vol 41, No 3 (2023): Desember Vol 41, No 2 (2023): Agustus Vol 41, No 1 (2023): April Vol 40, No 3 (2022): Desember Vol 40, No 2 (2022): Agustus Vol 40, No 1 (2022): April Vol 39, No 3 (2021): Desember Vol 39, No 2 (2021): Agustus Vol 39, No 1 (2021): April Vol 38, No 3 (2020): Desember Vol 38, No 2 (2020): Agustus Vol 38, No 1 (2020): April Vol 37, No 2 (2019): Desember Vol 37, No 1 (2019): Juni Vol 36, No 2 (2018): Desember Vol 36, No 1 (2018): Juni Vol 35, No 2 (2017): Desember Vol 35, No 1 (2017): Juni Vol 34, No 2 (2016): Desember Vol 34, No 1 (2016): Juni Vol 33, No 2 (2015): Desember Vol 33, No 1 (2015): JUNI Vol 32, No 2 (2014): DESEMBER Vol 32, No 1 (2014): JUNI Vol 31, No 2 (2013): DESEMBER Vol 31, No 1 (2013): JULI Vol 30, No 2 (2012): DESEMBER Vol 30, No 1 (2012): JUNI Vol 29, No 2 (2011): DESEMBER Vol 29, No 1 (2011): JUNI Vol 28, No 2 (2010): DESEMBER Vol 28, No 1 (2010): JUNI Vol 27, No 2 (2009): DESEMBER Vol 27, No 1 (2009): JUNI Vol 26, No 2 (2008): DESEMBER Vol 26, No 1 (2008): JUNI Vol 25, No 2 (2007): DESEMBER Vol 25, No 1 (2007): JUNI Vol 24, No 2 (2006): DESEMBER Vol 24, No 1 (2006): JUNI Vol 23, No 2 (2005): DESEMBER Vol 23, No 1 (2005): JUNI Vol 22, No 2 (2004): DESEMBER Vol 22, No 1 (2004): Juli Vol 21, No 2 (2003): DESEMBER Vol 21, No 1 (2003): JULI Vol 20, No 2 (2002): Desember Vol 20, No 1 (2002): Juli Vol 19, No 2 (2001): DESEMBER Vol 18, No 1&2 (2000) Vol 18, No 2 (2000) Vol 18, No 1 (2000) Vol 17, No 1 (1999) Vol 16, No 2 (1999) Vol 16, No 1 (1998) Vol 15, No 1&2 (1996) Vol 14, No 2 (1995) More Issue