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INDONESIA
Jurnal Sain Veteriner
Published by Universitas Gadjah Mada
ISSN : 012660421     EISSN : 24073733     DOI : -
Core Subject : Health,
Veterinary
Arjuna Subject : -
Articles 14 Documents
 1   2 
Search results for , issue "Vol 30, No 1 (2012): JUNI" : 14 Documents clear
Factors Associated with Occurrence of Campylobacteriosis on Village Chicken in Mataram Anwar Rosyidi; Setyawan Budiharta; Doddi Yudhabuntara
Jurnal Sain Veteriner Vol 30, No 1 (2012): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (501.329 KB) | DOI: 10.22146/jsv.2484

Abstract

Campylobacter jejuni is responsible for about 90% of cases of campylobacteriosis in human with gastroenteritis. Efforts to prevent the incidence of campylobacteriosis in human should be started with anassessment on its level in poultry. This study aims to determine the factors associated with the presence of C. jejuni on village chicken in Mataram, Nusa Tenggara barat. Association between C. jejuni as the dependent variable with independent variables were analyzed using logistic regression. Prevalence of C. jejuni from a total of 216 samples in Mataram at chicken level was 35.6% and while at the farm level was 70.5% (31/44). Variables showed a positive association of C. jejuni occurrence in chicken consist of age of chicken more than 3 months, the number of chicken ownership, and cage with soil floor. While the variable showed a negative association to the incidence of C. jejuni was drinking water of chicken originated from wells. Model obtained using logistic regression in association with occurence of C. jejuni on village chicken in Mataram was Y = -2.033 + 0.271 (age of chicken more than 3 month) + 0.103 (number of chicken ownership) + 0.741 (cage with soil floor) - 0.890 (drinking water of chicken originated from wells)Keywords: Campylobacter jejuni, logistic regression, village chicken, drinking water, wells
Expression of Bone Morphogenetic Protein-2 forMeasuring Effectiveness of Freeze Dried Bovine Bone Xenografts (FDBBX) Biomaterials as Bone ConnectorMaterial Agus Purnomo; Dhirgo Adji
Jurnal Sain Veteriner Vol 30, No 1 (2012): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5524.212 KB) | DOI: 10.22146/jsv.2486

Abstract

Osteogenic activity  in  normal fracture  healing will start  early  at  around  1 week after  the  repair ,  but not necessarily occur in fracture healing with the aid of grafts. Demineralized freeze dried bovine bone xenograft (DFDBBX) is a bone graft material made from cows that are    widely produced recently to assist the process of bone  grafting  in  humans,  but  has  not  been  used  on  animals.  It  does  not  know  how  it  works  in  helping  to  repair  the bone.  T wenty male SD rats  weighing 150 g, were divided into  2 groups of 10 rats  each. Group I  was the  control group, the  rats  were fractured  and reconnected  immediately  using intramedullar  pin fixation,  Group II  was a group  of  rats  that  were    fractured  and  reconnected  immediately  using  pin  fixation  intramedular  plus  graft material of freeze dried bovine bone xenograft. Fracture of the left femur was made in diaphysis part of bone. Evaluation  of  joints  performed  regularly  at  1  week  ,  2  weeks,  4  weeks,  6  weeks  and  8  weeks  post  surgery respectively by selecting at random, each group of 2 rats. Rats at each observation were collected two  head / group,      rats    were  then  killed,  the  bone  parts  to  be  joined  was  taken  and  stored  in  10%  of  formalin  for histopathologic  examination.  Some  pieces  of  the  bones  were  used  for  the  analysis  of  bone  morphogenetic  protein 2 (BMP-2 ) by immunohistochemical method. The results were then analyzed descriptively to see the changes directly on the X-rays and tissue. Overall results of this study showed that the graft material of DFDBBX were osteocondusive  rather  than osteoinductive  properties.  Nevertheless,  the  biomaterial  is  useful  for  orthopedic purposes  primarily  of  handling  cases  with    partial  fracture  of  bone  tissue  which  is  lost.  Due  to  the  nature osteocondusive  of  DFDBBX  will  make  cells  to  grow  and  to  unify  the  separate  bone  fragments.Keywords  :  fracture,  osteogenic  activity ,  demineralized  freeze  dried  bovine  bone  xenograft,  bone morphogenetic  protein-2,  immunohistochemical  analysis
Immunocytochemistry Streptavidin Biotin: Early Detection of Viral Nervous Necrosis Virus in the mucous of the Ikan Kerapu Macan(Epinephelus fuscoguttatus) Putu Eka Sudaryatma; Artanti Tri Lestari; Ni Luh Sunarsih; Ketut Sri Widiarti; Sulis NurHidayah; Didik Srinoto Srinoto
Jurnal Sain Veteriner Vol 30, No 1 (2012): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (8307.831 KB) | DOI: 10.22146/jsv.2487

Abstract

Kerapu macan  (Epinephelus fuscoguttatus)    is  one of the  kerapu  fishes  that  has been successfully  bred by the  farmers.  The  survival  rate  of  the  fish  reaches  up  to  the  40%  at  the  fish  hatchery  of  the  home  industry  of  in  Bali. The disease in which may cause significant mortality in kerapu fish, especially for the larvae and juveniles is viral nervous necrosis (VNN). We therefore developed and applied the immunological diagnostic approach of immunocytochemistry technique of streptavidin biotin (SB) for early detection of  VNN. Mucous samples of VNN  infected-  kerapu  fish  in  vivo  were   firstly  detected  by  reverse  transcriptase-polymerase  chain  reaction  (R T -PCR)  for  the  presence  of  DNA  VNN.  If  it  is  RT -PCR-positive  VNN,  the  mucous  samples  were  then  tested  by  SB. Results of the present study indicated that the VNN virus could be detected with SB technique within only 24 hours post infection.  It  was concluded that  rapid  and accurate SB technique  is  suitable and appropriate to  be applied  for  routine  control  and  prevention  national  program  in  the  Fish  Quarantine  for  Indonesia  because  no  need of  fish  sacrification,  and  scientific,  law  and  international    accepted,  and  even  no  hazardous  environmental contamination.Keywords:  kerapu  macan  (Epinephelus  fuscoguttatus),VNN,  mucous,  SB,  early  detection
Herd Immunity Against Rabies Among Dogs in Ambon Astri D T agueha D Tagueha; Setyawan Budiharta; Heru Susetyo
Jurnal Sain Veteriner Vol 30, No 1 (2012): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (501.329 KB) | DOI: 10.22146/jsv.2488

Abstract

The objectives of this study were to reveals factors associated with level of herd immunity against rabies. Four hundred and eighteen blood samples were collected from five subdistricts and 14 villages using stratified and  household  cluster  methods. The  result  in  ELISA  test  is    ≥  0,5  IU/ml,  dog  titers  sample  was  stated  as protective  to  rabies.  The result  showed  that  the  prevalence  of  protective  antibody  titers  was very  low  i.e.  3.35%. Linear regression  analysis reveals  subdistrict of    Nusaniwe, subdistrict of Baguala, dog owner education, post-vaccination  period  of  0  -  6  months, sex,  and  dogs  kept  as  a  guard  house  contribute  to  protective  level  of  antibody titers. Unweighted logistic regression revealed that subdistrict of Baguala (OR = 0.05), subdistrict of Sirimau (OR = 0.09), dog kept as a guard house (OR = 3.96), dog owner education (OR = 12.29), and post-vaccination period  of  0  -  6  months  (OR  =  27.08)  are  significantly  associated  with  dog's  immunity .  V accination  programs  need to  be  improved  by  considering  the  four  factors  that  emerged  in  these  two  regression  results.Keywords  :  rabies,  level  of  dog's  immunity ,  ELISA,  Ambon,  post-vaccination

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