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Ayyub Harly Nurung
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Editor's Address: 3rd Floor Laboratory Faculty of Pharmacy, 2nd Campus of UMI: Jl. Urip Sumoharjo km. 5 , Makassar, South Sulawesi, Indonesia
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INDONESIA
Journal Microbiology Science
Published by Universitas Muslim Indonesia
ISSN : 2808392X     EISSN : 28083911     DOI : https://doi.org/10.56711/jms
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology
Journal Microbiology Sciences (J. Mic. Sciences) receives writing in the form of research results, scientific studies and brief descriptions relating to Microbiology, such as Molecular and Microbial Epidemiology Clinical Microbiology Medical Microbiology Pharmaceutical Biotechnology and others related to Microbiology Sciencee
Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi dan Bioteknologi Terapan
Articles 5 Documents
 1 
Search results for , issue "Vol 3, No 2 (2023)" : 5 Documents clear
Antibacterial Activity of Compound TL-4 IFATL-05 Isolate Endophytic Fungi from Tunjuk Langit Root (Helminthostachys zeylanica (L.) Hook) Against Bacterial Gastrointestinal Tract Infection Yuridu, Fatur Rahmansyah; Herwin, Herwin; Fitriana, Fitriana
Journal Microbiology Science Vol 3, No 2 (2023)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v3i2.983

Abstract

Tunjuk Langit Plant (Helminthostachys zeylanica (L.) Hook) contains a lot of secondary metabolite compounds, one of which is found in the root which contain the flavonoids ugonin and quercetin. The purpose of this study was to determine the antibacterial activity of compound 4 isolates IFATL-05 endophytic fungi from Tunjuk Langit (Helminthostachys zeylanica (L.) Hook) root against bacterial gastrointestinal tract infection. The results of purification of endophytic fungi with IFATL-05 code on macroscopic examination showed blackish-white fungus color, round with scalloped colony shapes, wavy edges and drop-like elevation angles. The process of separating the compounds using KLTP with n-hexane:ethyl acetate (1:1) as an eluent resulted in four bands of active compounds in UV lamps of 254 and 366 nm (TL-1, TL-2, TL-3 and TL-4). Test results for compound 4 isolates of IFATL05 endophytic fungi from Tunjuk Langit root (Helminthostachys zeylanica (L.) Hook) using the Bioautographic TLC method using n-hexane:ethyl acetate (6:1) as eluent on Escherichia coli, Salmonella thypi, Shigella dysenteriae and Vibrio cholerae with Rf value = 0.90
Phytochemical Screening And Activity Test of Maja Fruit Extract (Aegle Marmelos L.) on Growth Salmonella typhi and Vibrio cholerae Yusuf, Nur Annah Achriana; Rannah, St.; Salasa, Alfrida Monica
Journal Microbiology Science Vol 3, No 2 (2023)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v3i2.974

Abstract

Maja fruit (Aegle marmelos L.) contains secondary metabolites that have antibacterial activity in the form of steroids, flavonoids and saponins, which are not used optimally. The target of this study was to observe the antibacterial activity of Maja fruit (Aegle armelos L.) extract on Vibrio cholera and Salmonella typhi. The extraction method formed by maceration and using 96% ethanol solution then the antibacterial activity was tested on the agar diffusion technique. The results of the study show that the range of inhibition zone diameter (mm) is gradual for the Maja Fruit (Aegle marmelos L.) extract test at concentrations of 2%, 4%, 8%. For Salmonella typhi was 13 mm;16,3 mm;19 mm but ciprofloxacin as a positive control was 57mm and DMSO as a negative control did not show any inhibition zone. However, the diameter of the inhibition zone in Vibrio cholera was found to be in the range of 13 mm;18.3 mm;19.6 mm and the positive control ciprofloxacin was 32.3 mm , but the negative control did not have an inhibiting zone. The results of statistical tests showed that Maja fruit (Aegle marmelos L.)  extract had antibacterial activity on Vibrio cholerae and Salmonella typhi at the maximum concentration of 2% w/v (P <0.05).
Antibacterial Activity of Extra Ethanol Kopasanda Leaves (Chromolaena Odorate L.) Against Pathogenic Bacteria of Urinary Tract Infection by TLC-Bioautography and Agar Diffusion Wirawan, Alif Yusra; Kosman, Rachmat; Herwin, Herwin
Journal Microbiology Science Vol 3, No 2 (2023)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v3i2.979

Abstract

Kopasanda  (Chromolaena odorata L.) leaves are one of the plants (herbs) containing various types of secondary metabolites, including saponins, tannins, alkaloids, and flavonoids, which function as antibacterial agents used as wound medicine for the community. This study aims to determine the antibacterial activity of the ethanol extract of Kopasanda (Chromolaena odorate L.) leaves using the TLC-bioautography and diffusion methods. The results of the antibacterial activity screening test obtained the active extract at a concentration of 0.1% against Escherichia coli and Staphylococcus aureus. The results of the antibacterial activity test using the TLC-Bioautography method with the eluent N-Hexane:Ethyl Acetate (4:1) showed results from Staphylococcus aureus bacteria with Rf values = 0.90, 0.78, 0.67, 0.52, 0.38, 0.27, 0.14, 0.05, and Escherichia coli bacteria with Rf values = 0.90, 0.52, 0.38, 0.27, 0.14, 0.05. Based on the results of testing the antibacterial activity with the agar diffusion method, the largest diameter of the inhibition zone at a concentration of 10% was obtained for Escherichia coli bacteria with an inhibition zone diameter of 13.59 mm, and Staphylococcus aureus with an inhibition zone diameter of 15.52 mm.
Antibacterial Activity of Ethanol Extract of Qust Al Hindi Plant Root (Saussurea Lappa) Against Bacteria Staphylococcus aureus and Escherichia coli by TLC-Bioautography Muthia, Farah; Sukmawati, Sukmawati; Fitriana, Fitriana
Journal Microbiology Science Vol 3, No 2 (2023)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v3i2.980

Abstract

The Indian Costus plant (Saussurea lappa) possesses the ability as an antibacterial, which contains alkaloid compounds, whereas this study aims to determine the antibacterial activity of ethanol extract of Indian Costus plant root (Saussurea lappa). The extraction process of Indian Costus plant root simplicia (Saussurea lappa) was carried out using the Soxhlet method with 96% ethanol as a solvent, and then evaporated with a rotavapor to obtain a viscous extract. Screening tests were carried out by giving ethanol extract of Indian Costus plant root (Saussurea lappa) with a concentration of 0.1% to 2 test bacteria, namely Staphylococcus aureus and Escherichia coli. Thereafter, the Thin Layer Chromatography-Bioautography test was carried out, followed by identification of chemical compounds. The screening test results obtained showed that the ethanol extract of Indian Costus plant root (Saussurea lappa) provided activity against 2 test bacteria at a concentration of 0.1%. The Thin Layer Chromatography-Bioautography test results indicated that there were 3 spots that were formed with Rf values of 0.67, 0.60, 0.54, 0.45, 0.43 providing activity on Staphylococcus aureus and Escherichia coli bacteria. The chemical identification of ethanol extract of Indian Costus plant roots (Saussurea lappa) yeilded positive result for the presence of alkaloids. Based on the activity of the ethanol extract of Indian Costus plant root (Saussurea lappa), it is known that it has potential as an antibacterial.
Antibacterial Activity of Ethanol Extract of Purple Kencana Leaves (Ruellia tuberosa L.) Using TLC-Bioautography and Agar Diffusion Methods Sari, Felisa Ananda; Herwin, Herwin; Handayani, Selpida
Journal Microbiology Science Vol 3, No 2 (2023)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v3i2.982

Abstract

Kencana Ungu Leaf (Ruellia tuberosa L.) is a traditional plant that has antibacterial activity. The purpose of this study was to determine the antibacterial activity of the ethanol extract of Kencana Ungu leaves (Ruellia tuberosa L.) using TLC-Bioautography and Agar Diffusion methods. Simplisia from Kencana Ungu leaves (Ruellia tuberosa L.) was extracted by maceration method and then evaporated to obtain a thick extract. Preliminary testing, namely the antibacterial screening test, active extract at a concentration of 0.5% against Escherichia coli, Shigella dysenteriae, and Salmonella thypi. The results of the antibacterial activity test using the TLC-Bioautography method using chloroform: methanol (7:1) as an eluent showed results with an Rf value of 0.89 which inhibited the growth of Escherichia coli, Shigella dysenteriae, and Salmonella thypi bacteria. While the results of testing the antibacterial activity using the Agar Diffusion method obtained the largest diameter of the inhibition zone at a concentration of 20% for Escherichia coli bacteria with an inhibition zone diameter of 12.15 mm, Shigella dysenteriae with an inhibition zone diameter of 11.72 mm, and Salmonella thypi with an inhibition zone diameter of 12.67 mm

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