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Manilkara: Journal of Bioscience
Published by Universitas Terbuka
ISSN : -     EISSN : 29640164     DOI : https://doi.org/10.33830/manilkara
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience Veterinary
MANILKARA: Journal of Bioscience is designed to share research articles from academics, researchers, practitioners, and students on research in various aspects of biology and its applications. Papers for publication in this journal are selected through proper peer-review to ensure quality, originality, suitability, and legibility. This journal covers various disciplines in biology (microbiology, genetics, biochemistry, physiology, ecology, taxonomy, structure and development of animals/plants, and evolution); Biotechnology; and applied science in solving problems in the fields of biology (biomedical, agricultural, industrial, ethnobiological, and environmental). The journal is published biannually on February and August.
Arjuna Subject : Umum - Umum
Articles 5 Documents
 1 
Search results for , issue "Vol 1 No 1 (2022): August" : 5 Documents clear
Modifikasi Metode Preparasi Pewarnaan Akar untuk Deteksi dan Visualisasi Pembentukan Koloni Fungi Mikoriza Arbuskula (FMA) Susan Irmayanti; Inggit Winarni
MANILKARA: Journal of Bioscience Vol 1 No 1 (2022): August
Publisher : LPPM Universitas Terbuka

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (717.497 KB) | DOI: 10.33830/manilkara.v1i1.3166.2022

Abstract

The root staining method was used to detect the presence of Arbuscular Mycorrhizal Fungi (AMF) and to calculate the colonization of the AMF in the roots. Optimal root staining methods not only help in the study of plant-associated mycorrhizae, but also save time and money. Root staining aims to clarify and sharpen the picture of AMF associated with plants on the roots, making it easier to observe with a light microscope. This study aims to obtain an effective (fast, safe, and economical) and flexible method of preparation of root staining so that the visualization of AMF on roots becomes clear and contrasting. The method used in this research is descriptive qualitative which includes the cleanliness of the roots from cell contents, root texture, and color contrast, which consists of three treatments, namely P1 (control) using 10% KOH with 90°C heating and followed by the modified Philips & Hayman staining procedure (1970), P2 using 5% KOH by heating at 90°C followed by the modified Philips & Hayman (1970) procedure using 1% HCl, and P3 using 5% KOH heating at 90°C then stained followed the staining method of Philips & Hayman (1970) which was modified by the use of commercial vinegar as a substitute for HCl. All treatments used Trypan Blue dye. The results showed that the P2 and P3 treatments showed results that were not different from P1, the state of the roots is clean enough thereby detection and visualization of AMF could still be observed properly. The P3 treatment method with heating for 9 minutes can be an alternative method that is effective (fast, safe, economical) and flexible. It faster than common method because it takes 9 minutes for cleaning root cells, relatively safe with the use of commercial vinegar solution to replace HCl solution, and economical because it can reduce the need for KOH material up to 50%.
Aktivitas Antibakteri Ekstrak Etanolik Daun Jeruk (Citrus nobilis, Citrus sinensis, dan Citrus maxima) Terhadap Bakteri Staphylococcus aureus dan Pseudomonas aeruginosa Ifandari; einstivina nuryandani
MANILKARA: Journal of Bioscience Vol 1 No 1 (2022): August
Publisher : LPPM Universitas Terbuka

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (396.583 KB) | DOI: 10.33830/manilkara.v1i1.3168.2022

Abstract

Some bacteria that live on body surfaces such as Staphylococcus aureus and Pseudomonas aeruginosa can be pathogenic to human. These bacteria are known to be resistant to certain antibiotics. Therefore, it is necessary to develop other alternative materials as safe antibacterial agents. One of the natural ingredients that have potential as antibacterial is citrus leaf. The aim of this study was to determine the inhibitory power of citrus leaf extracts from three types of citruses (Citrus nobilis, Citrus sinensis, and Citrus maxima) against the growth of S. aureus and P. aeruginosa bacteria. This study used the paper disk diffusion method with a percentage of 12.5%, 25%, and 50% of the extract dissolved in 2% carboxymethyl cellulose (CMC). The negative control used 2% CMC, while the positive control used the antibiotic ciprofloxacin. The results showed that the antibacterial activity of the three extracts was significantly different from the negative control, but the activity between the three types of citrus leaf extract was not significantly different. C. nobilis leaf extract with a concentration of 50% had antibacterial activity to Staphylococcus aureus relatively higher than the other two citrus leaf extracts, while in P. aeruginosa, the antibacterial activity of C. maxima leaf extract was relatively higher than other citrus leaf extracts. These results indicated that citrus leaves extracts from C. nobilis, C. sinensis, and C. maxima had inhibitory effects on S. aureus and P. aeruginosa growth.
Keanekaragaman Makrozoobentos Sebagai Bioindikator di Perairan Lentik Kawasan Rawa Jombor Klaten Jawa Tengah Sandhie Budi Himawan; Yuni Tri Hewindati
MANILKARA: Journal of Bioscience Vol 1 No 1 (2022): August
Publisher : LPPM Universitas Terbuka

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (447.108 KB) | DOI: 10.33830/manilkara.v1i1.3394.2022

Abstract

Organisms that live permanently on the bottom of lentic or still waters, such as macrozoobenthos, are very susceptible to changes in water quality that can have an impact on their composition and abundance. Because of their tolerance to environmental changes, these organisms are often used as an indicator in physical and chemical changes in the aquatic environment. Rawa Jombor or Jombor swamp is a lentic freshwater in Krakitan Village, Bayat District, Klaten, Central Java. In addition to tourism, the surrounding community uses Rawa Jombor in their daily activities for floating market and fish cages. The existence of these various activities can produce waste and garbage which then settles to the bottom of the waters and has an impact on reducing water quality. This study aims to examine the diversity and distribution of macrozoobenthos. Water quality was measured by taking samples at three locations, namely station I (littoral zone), station II (limnetic zone), and station III (benthic zone). Sampling at each station used a Van Veen grab sediment sampler. The Macrozoobenthos Diversity Index was determined using the Shannon & Wiener formula. The diversity index values for stations I, II, and III are 1.579; 1.566; and 1.785, respectively, with moderate levels of community diversity. 14 types of macrozoobenthos were identified, of which Tubifex tubifex was the most abundant species in station 1 and station 2. Meanwhile at station 3 is dominated by Anentome Helena. Based on the analysis of organism diversity and the results of water quality inspection in our study, it shows that the water quality in the Jombor swamp is classified as moderately polluted.
Potensi Ekosistem Terumbu Karang di Taman Wisata Perairan Gili Sulat dan Gili Lawang Lombok Timur Akhyar, Lalu Ainul; Prasetyo, Budi
MANILKARA: Journal of Bioscience Vol 1 No 1 (2022): August
Publisher : LPPM Universitas Terbuka

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1251.001 KB) | DOI: 10.33830/manilkara.v1i1.3401.2022

Abstract

Coral reefs are one of the ecosystems that play an important role in coastal areas but are vulnerable to change. Ecologically, coral reefs act as habitats for various kinds of reef fish, in addition to functioning as beach protectors from the crashing waves of currents as well as a source of germplasm. The diversity of coral genera and other biota is one of the important indicators in supporting the sustainability of water tourism activities, especially snorkeling and diving. Areas that have relatively high diversity of coral reefs have more resilience in their role in protecting coastal areas so that their existence needs to be maintained in order to avoid extinction. This study aims to identify the names of coral genera and measure the percentage of coral cover in the Gili Sulat and Gili Lawang Water Tourism Parks (WTP). The method used in this research is the Point Intercept Transect (PIT) method and the Time Swim method. The results showed that 10 genera had been identified, namely the genus Acropora, Euphyllia, Fungia, Galaxea, Lobophyllia, Montastrea, Montipora, Pachyseris, Plerogyra, and Porites. The largest coral genera were Porites and Acropora which were spread on transects 1 and 7. The average coverage of the two corals was 32.34% in Gili Sulat and 39.64% in Gili Lawang.
Variabilitas Morfologi Jagung di Kecamatan Ciseeng, Kabupaten Bogor dan Kecamatan Setu, Kota Tangerang Selatan Diki; Susi Sulistiana; Arif Cahyani Ilyas
MANILKARA: Journal of Bioscience Vol 1 No 1 (2022): August
Publisher : LPPM Universitas Terbuka

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (738.556 KB) | DOI: 10.33830/manilkara.v1i1.3546.2022

Abstract

Penelitian ini mengukur dan membandingkan variabilitas morfologi dari dua populasi jagung varietas Golden di Kabupaten Bogor dan Kota Tangerang Selatan dengan mengukur parameter morfologi tanaman jagung yaitu: jumlah tongkol panen, tinggi tanaman, tinggi letak tongkol, panjang tongkol, diameter tongkol, kadar air saat panen dan bobot kering 100 biji. Pupuk yang digunakan berbeda, untuk tanaman jagung di lokasi kota Tangerang Selatan menggunakan pupuk organik yaitu pupuk kandang kambing, sedangkan lokasi kabupaten Bogor menggunakan pupuk anorganik yaitu pupuk urea, TSP, KCl (NPK). Untuk menganalisis data variabilitas menggunakan Anova. Berdasarkan hasil pengamatan, bahwa secara umum terdapat perbedaan dalam morfologi jagung diantara kedua lokasi tersebut. Morfologi jagung di lokasi kota Tangerang Selatan dengan kabupaten Bogor berbeda terutama dalam hal, variabel tinggi tongkol, diameter tongkol, bobot 100 biji, bobot tongkol, dan kadar air. Perbedaan hasil tersebut diduga karena penggunaan pupuk yang berbeda.

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