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Syamsurizal
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Genetikawan Muda Indonesia (GeMI) Jl. Prof Dr Hamka, Air Tawar, Padang, Sumatera Barat, Indonesia, 27171 Email: tropicalgenetics.gemi@gmail.com Telepon: +628126709150
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INDONESIA
Tropical Genetics
Published by Genetikawan Muda Indonesia
ISSN : -     EISSN : 27984710     DOI : 10.24036/tg.v3i2.38
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
The scope of this journal is; Genetics Basics, Classical Genetics, Theoretical and Applied Genetics, Molecular Genetics, Cytogenetics, Bioinformatics, Genetic Diversity, Population Genetics, Genetic Engineering, Forensic Genetics, Behavioural Genetics, Advanced Genetics and Evolution, Clinical Genetics and Genetic Counselling Genomics, Biotechnology and Genetics Education.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 6 Documents
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Search results for , issue "Vol. 1 No. 2 (2021)" : 6 Documents clear
Pedigree analysis of diabetes mellitus in Minangkabau ethnic S. Syamsurizal; Siti Halifah; Elsa Badriyya
Tropical Genetics Vol. 1 No. 2 (2021)
Publisher : Genetikawan Muda Indonesia

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Abstract

Diabetes mellitus has a tendency to be inherited. The inheritance pattern of diabetes mellitus in the Minangkabau ethnic group follows the mitochondrial and autosomal inheritance pattern. Patients with diabetes mellitus in the Minangkabau ethnicity have a equal distribution between men and women. Patients with diabetes mellitus in this ethnic group generally know that they suffer from diabetes mellitus in old age or after other complications arise due to diabetes mellitus.
Genetic diversity of gamma ray aplication result on Mandarin’s SoE using ISSR markers Putri Kendari; Mahfut Mahfut
Tropical Genetics Vol. 1 No. 2 (2021)
Publisher : Genetikawan Muda Indonesia

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Abstract

Mandarin’s SoEare one of Indonesia's leading mandarinto substitute imported oranges. The quality of oranges can be improved through breeding programs, one of which is mutation breeding using gamma rays. The purpose of this study was to obtain information about the genetic variation of SoE mandarin resulting from gamma ray radiation using ISSR markers. PCR results with ISSR markers on tangerine plants produced by gamma ray radiation showed various patterns, namely the bands were the same, lost bands, and experienced the addition of new bands compared to control plants.
Primer design, in silico PCR and optimum annealing temperature for Escherichia coli detection in refillable drinking water samples Afifatul Achyar; Annisa Irna Putri; Dwi Hilda Putri; Yuni Ahda
Tropical Genetics Vol. 1 No. 2 (2021)
Publisher : Genetikawan Muda Indonesia

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Abstract

Refill Drinking Water Depots (DAMIU) in the community are easy to find at affordable prices, which is a concern in the feasibility of refill drinking water quality. E.coli is one of the pathogenic bacteria present in drinking water that has poor quality. When it enters the body it can cause symptoms of diarrhea, fever, vomiting and others. The purpose of this study was to create a specific primer for E.coli that can be used to detect E.coli and determine the optimum temperature of primer annealing. Researchers do a new primer design because the existing design does not necessarily produce the same results due to various factors of different experimental conditions. The primer design was carried out on an in silico-based and had to meet the criteria for a good primer because it would be used in vitro. E.coli gene sequences was aligned with Shigella sp. using Pairwise Alignment. Primer candidates were analyzed using NCBI's Primer3 and Geneious Prime tools. The result is that the first primer pair is forward 5'–ATGCAGTGGTTCCTTATCTCACA-3' reverse 5'- ATCCTTAATGGCACTGCGCT-3', amplifying the amplicons along 417 bp in the yraJ gene. The second primer pair, forward 5'-CAGAACGTTTTTCATTCAGCAGG-3' reverse 5'-GCCACTACCAGATCGAGTCA-3' amplifies the 573 bp amplicon in the rne gene. The optimum annealing temperature for both pairs of primers was 59.5oC.
Mapping and distribution of the telomeric sequences (T2AG3 repeats) in the marsupialia famili Macropodidae Rony Kunda; Mechiavel Moniharapon; Pieter Kakisina
Tropical Genetics Vol. 1 No. 2 (2021)
Publisher : Genetikawan Muda Indonesia

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Abstract

Marsupial and monotreme mammals very important in vertebrate phylogeny studies between reptile-mammal divergence 310 million years ago (mya) and the eutherian (placental) mammal radiation 105 mya. They have many features including their distinctive chromosomes, which in marsupials are typically very large and well conserved between species. Monotreme genomes are divided into several large chromosomes and many smaller chromosomes, with a complicated sex chromosome models that forms a translocation chain in male meiosis. In Macropus genera members, telomeres have been shown to be involved in the fusion and inversion of chromosomes, but for Kangaroo, the telomere sequence remains consistent and does not change during the chromosomal fusion event, but this is not the case in mice. The ancestor of marsupials (plesiomorphic), has a karyotype of 2n=14, but for kangaroos and wallabies have a karyotype of 2n=22, based on fews curently research, we asserted that this karyotype is derived from the karyotype of marsupial ancestors who have a karyotype of 2n=14. Based on molecular genetic studies have shown that W. bicolor (swamp wallaby) is more appropriately grouped into the Macropus genera, not as a “sister” of Macropus genera. In addition Macropus and W. bicolor there has been a division of chromosomes due to chromosome fusion, but W. bicolor has fewer chromosomes than other members of Macropodidae, i.e (2n=10 for females, and 2n=11 for males).
Maternal genetic variation of Betawi population Wolly Candramila; Bambang Suryobroto
Tropical Genetics Vol. 1 No. 2 (2021)
Publisher : Genetikawan Muda Indonesia

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Abstract

Betawi identity was assessed based on the diversity of maternal lines using genetic markers in the D-loop region of mitochondrial DNA. The results of the cleavage by the restriction enzymes DdeI, HaeII, HaeIII, and HinfI showed five patterns of genetic variants which were later named Haplotype I, II, III, IV, and V. Haplotype I had the highest frequency of 75.6%, followed by Haplotype III 11.5%, and Haplotype II 10.3%. Haplotypes IV and V were only found in 1 individual each and were assumed to be individual mutations. The highest haplotype in the Betawi population showed the same restriction enzyme cleavage pattern with all comparison samples from the Sunda and Malay populations. Meanwhile, Haplotype II and III were strongly suspected as the result of the divergence of Haplotype I and can also be found in other ethnic groups in Indonesia. This finding confirms Jakarta as a location for ethnic mixing which then produces a new local culture as Betawi Culture.
Molecular assessment of native fish diversity in UNESCO heritage site, Tasik Raban, Malaysia using DNA barcoding: DNA Barcoding of Native Fishes in Tasik Raban, Malaysia Ramizah Abdull Rahman; Adibah Abu Bakar; Nurul Fizatul Nabilah Osman; Syazwan Saidin; Intan Faraha A. Ghani
Tropical Genetics Vol. 1 No. 2 (2021)
Publisher : Genetikawan Muda Indonesia

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Abstract

Despite the fact that freshwater fishes have been studied for over a century, Malaysia's conservation status and management are still in their infancy. The poor progress of freshwater fish taxonomy and conservatory management is primarily due to a lack of interest and funding. There are still numerous unsolved taxonomic issues of freshwater fishes in Malaysia and this had a negative impact on national ichthyological research. As a result, the current research aims to aid the success of the molecular DNA barcode project, particularly in inland reservoirs such as Tasik Raban, Perak which is located in the UNESCO Heritage Site. The Cytochrome c oxidase subunit 1 (COI) marker was used in this project to ensure that native fishes were taxonomically and molecularly barcoded and ready to be accessible through internet databases. Such public references can aid in raising awareness about the management of local fish variety. Taxonomy and molecular characterization data can be utilized to plan future conservation efforts, particularly for depleted, unknown, or cryptic native species.

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