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Mahfut
Jurusan Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Lampung, Jalan Prof. Dr. Sumantri Brojonegoro No. 1 Bandar Lampung, Lampung 35144
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Analisis Kualitas Limbah Cair Pada Kolam Anaerob IV di Instalasi Pengolahan Air Limbah (IPAL) PT. Perkebunan Nusantara VII (Persero) Unit Usaha Bekri K, Mahfut
Biogenesis Vol 1, No 2 (2013)
Publisher : Jurusan Biologi UIN Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Among the industries in Lampung, PT. Perkebunan Nusantara VII (Persero) Bekri Business Unit is one of the Palm Oil Processing Plant (POPP) industry who spend a lot of waste water in the form of residual organic liquid materials. The rest of the liquid material can cause problems such as pollution so it needed special treatment before the waste water is discharged into the environment. This study was conducted to determine the quality of liquid waste in an anaerobic pool IV on Wastewater Treatment Plant (WWTP) PT. Plantation Nusantara VII (Persero) Business Unit Bekri so it can be determined whether the waste water treatment process can improve the quality of waste water for the better. Waste water quality measurement is done using the parameters of physics,chemistry, and heavy metals, namely pH, COD, BOD5, TSS, Cd, Cu, Pb, and Zn. The results showed that the quality of the wastewater in an anaerobic pool IV was good and meets the quality standards of palm oil mill effluent by the State Minister of Environment Resolution No. 03 / MENKLH /11/1991 and the Indonesian Government Regulation No. 20 of 1990 on Water Pollution Control. It also indicates that the effluent treatment system at the Wastewater Treatment Plant (WWTP) PT. Perkebunan Nusantara VII (Persero) Bekri Business Unit is very effective.Keywords: anaerobic pool, liquid waste, PTPN VII
Deteksi Odontoglossum ringspot virus Terhadap Anggrek Alam di Hutan Wonosadi, Gunung Kidul K, Mahfut; Setiadi Daryono, Budi
Biogenesis Vol 2, No 2 (2014)
Publisher : Jurusan Biologi UIN Alauddin Makassar

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Abstract

Wonosadi forest is managed forests by indigenous forest communities and is located in the hamlet of Duren and Sidorejo, Beji village, subdistrict Ngawen, Gunung Kidul regency, Yogyakarta. One of the biological richness contained in Wonosadi forest is very valuable natural orchids and need to be preserved. Management to the preservation of nature in the woods Wonosadi orchids can be done through natural orchid protection against infectious pathogens, including viruses. Odontoglossum ringspot virus (ORSV) is one type of orchid viruses infect most widely reported and has spread widely in the world, including in Indonesia. ORSV can cause severe damage to the orchid. Therefore, it is necessary to study to detect ORSV that infect wild orchids in the forest Wonosadi molecular basis for detecting the presence and pattern of infection ORSV as well as possible to reduce the severity of disease caused by infection with the virus. The purpose of this study is to assess the natural orchid ORSV infection in molecular Wonosadi forest that can be used as a database in an effort to protect against natural orchid Wonosadi forest. In the long term, the results of this study are very useful to support conservation efforts and determine appropriate conservation efforts for wild orchids in the forest Wonosadi. The initial phase of the study conducted a survey and collection of samples of wild orchids in the forest leaves that show symptoms of infection Wonosadi ORSV. Furthermore, molecular analysis performed with gene amplification Coat Protein ORSV using Reverse engineering Trancriptase Polymerase Chain Reaction (RT-PCR).Keywords: orchid soil, ORSV, RT-PCR, Wonosadi forest
Pengenalan Teknik Budidaya Kelengkeng Super Sleman Berbasis Lingkungan Mahfut, Mahfut; Wahyuningsih, Sri
Jurnal SOLMA Vol 8 No 2 (2019): Jurnal Solma
Publisher : Uhamka Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (624.307 KB) | DOI: 10.29405/solma.v8i2.3472

Abstract

Perubahan kenaikan temperatur udara sangat dirasakan masyarakat di sekitar Dusun Gejayan, Kecamatan Condong Catur, Kabupaten Sleman, D.I. Yogyakarta. Penanaman tanaman buah seperti Kelengkeng Super Sleman (KSS) menjadi alternatif dalam memecahkan permasalahan terkait mengurangi dampak pemanasan global melalui program penghijauan. Selain itu memiliki nilai tinggi baik dari sisi pemenuhan gizi maupun produk pemasaran di bidang ekonomi bagi masyarakat sekitar. Pengenalan teknik budidaya KSS berbasis lingkungan di Dusun Gejayan merupakan salah satu bentuk dukungan pembangunan yang berkesinambungan melalui program setiap daerah dalam menghasilkan suatu produk (one village one product). Kegiatan dilakukan melalui tiga tahapan, yaitu; penyuluhan, pelatihan, dan pendampingan secara berkala. Hasil yang diperoleh dalam penyuluhan sebagai kegiatan awal adalah terjadi peningkatan persentase pengetahuan peserta dengan nilai sebelum dan sesudah kegiatan adalah sebesar 69,72%. Hasil juga menunjukkan bahwa warga Padukuhan Gejayan secara umum telah mengalami perubahan paradigma terkait budidaya KSS dan memahami penerapan teknik budidaya KSS berbasis lingkungan. Dampak positif tidak hanya berapa dukungan penghijauan dan pemenuhan gizi warga, tetapi juga menambah perekonomian warga melalui penjualan buah dan bibit ke konsumen. Dengan dukungan penuh dari Kepala Padukuhan Gejayan, penanaman pohon KSS diharapkan terus berkembang dan menjadi suatu budaya di dalam lingkungan Padukuhan Gejayan.
Identification of dendrobium Natural Orchids in Liwa Botanical Garden based on leaf morphological characters Mahfut, Mahfut; Handayani, Tundjung Tripeni; Wahyuningsih, Sri; Sukimin, Sukimin
Jurnal Pemberdayaan: Publikasi Hasil Pengabdian Kepada Masyarakat Vol 5, No 1 (2021)
Publisher : Universitas Ahmad Dahlan, Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/jp.v5i1.2938

Abstract

Lampung Indonesia is one of the provinces on the island of Sumatra, which has a flora conservation area located in Liwa Botanical Garden, West Lampung Regency. One of the flora species in the area with a high level of diversity is the orchid plant. The Dendrobium is the largest orchid species, where the public widely hunts this orchid for an economic reason due to the beauty of its various flower shapes and colors. Given the high level of human encroachment, this can threaten the existence of natural orchid plants, causing loss of their original habitat and causing the extinction of orchid species. Based on data in Liwa Botanical Garden, there has been no further identification of natural orchids as a plant conservation effort. To protect and immediately save these natural orchids, it is necessary to identify the Dendrobium orchid species based on the leaf morphological approach. This research will be conducted in April-September 2020. Observations of leaf morphology include leaf shape, leaf tip shape, leaf base shape, leaf edge, leaf reinforcement, leaf arrangement, leaf surface texture, leaf symmetry, and leaf sitting based on guidelines for ornamental plant characterization books. Observations as secondary data were added to the character of the pseudobulb shape and orchid habitat. Observations as secondary data were added on the habitat character of pseudobulbs and orchids. The implementation of this activity is still in the period of the Covid-19 pandemic, so most of the activities are carried out online. The coaching and training phase was conducted through internal virtual discussions on Tuesday, July 7, 2020, while the coaching phase was conducted through meetings or verbal communication via telephone groups and WhatsApp. Based on the evaluation data, it is known that there is an increase in knowledge and understanding of training participants by 21.64 points. The percentage increase in value before and after training is 34.72%. Orchid-related activities at Liwa Botanical Garden were never carried out, thus imparting knowledge to Liwa Botanical Garden technicians and workers on the development of natural orchid identification methods based on the morphological character in local natural resource conservation in the 4.0 industrial revolution era.
Analisis Kualitas Limbah Cair Pada Kolam Anaerob IV di Instalasi Pengolahan Air Limbah (IPAL) PT. Perkebunan Nusantara VII (Persero) Unit Usaha Bekri Mahfut K
Biogenesis: Jurnal Ilmiah Biologi Vol 1 No 2 (2013)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v1i2.451

Abstract

Among the industries in Lampung, PT. Perkebunan Nusantara VII (Persero) Bekri Business Unit is one of the Palm Oil Processing Plant (POPP) industry who spend a lot of waste water in the form of residual organic liquid materials. The rest of the liquid material can cause problems such as pollution so it needed special treatment before the waste water is discharged into the environment. This study was conducted to determine the quality of liquid waste in an anaerobic pool IV on Wastewater Treatment Plant (WWTP) PT. Plantation Nusantara VII (Persero) Business Unit Bekri so it can be determined whether the waste water treatment process can improve the quality of waste water for the better. Waste water quality measurement is done using the parameters of physics,chemistry, and heavy metals, namely pH, COD, BOD5, TSS, Cd, Cu, Pb, and Zn. The results showed that the quality of the wastewater in an anaerobic pool IV was good and meets the quality standards of palm oil mill effluent by the State Minister of Environment Resolution No. 03 / MENKLH /11/1991 and the Indonesian Government Regulation No. 20 of 1990 on Water Pollution Control. It also indicates that the effluent treatment system at the Wastewater Treatment Plant (WWTP) PT. Perkebunan Nusantara VII (Persero) Bekri Business Unit is very effective.Keywords: anaerobic pool, liquid waste, PTPN VII
Deteksi Odontoglossum ringspot virus Terhadap Anggrek Alam di Hutan Wonosadi, Gunung Kidul Mahfut K; Budi Setiadi Daryono
Biogenesis: Jurnal Ilmiah Biologi Vol 2 No 2 (2014)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v2i2.475

Abstract

Wonosadi forest is managed forests by indigenous forest communities and is located in the hamlet of Duren and Sidorejo, Beji village, subdistrict Ngawen, Gunung Kidul regency, Yogyakarta. One of the biological richness contained in Wonosadi forest is very valuable natural orchids and need to be preserved. Management to the preservation of nature in the woods Wonosadi orchids can be done through natural orchid protection against infectious pathogens, including viruses. Odontoglossum ringspot virus (ORSV) is one type of orchid viruses infect most widely reported and has spread widely in the world, including in Indonesia. ORSV can cause severe damage to the orchid. Therefore, it is necessary to study to detect ORSV that infect wild orchids in the forest Wonosadi molecular basis for detecting the presence and pattern of infection ORSV as well as possible to reduce the severity of disease caused by infection with the virus. The purpose of this study is to assess the natural orchid ORSV infection in molecular Wonosadi forest that can be used as a database in an effort to protect against natural orchid Wonosadi forest. In the long term, the results of this study are very useful to support conservation efforts and determine appropriate conservation efforts for wild orchids in the forest Wonosadi. The initial phase of the study conducted a survey and collection of samples of wild orchids in the forest leaves that show symptoms of infection Wonosadi ORSV. Furthermore, molecular analysis performed with gene amplification Coat Protein ORSV using Reverse engineering Trancriptase Polymerase Chain Reaction (RT-PCR).Keywords: orchid soil, ORSV, RT-PCR, Wonosadi forest
APLIKASI FILOGENETIK DI DUNIA BIOLOGI KESEHATAN: MELACAK PANDEMI PATOGEN Mahfut Mahfut
Teknosains Vol 14 No 2 (2020)
Publisher : Fakultas Sains dan Teknologi Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/teknosains.v14i2.15406

Abstract

Phylogenetics is known as a study related to biological science. Phylogenetics provides facilities in the fields of human epidemiology, ecology, and evolutionary biology. In evolutionary science, this field of science is used to integrate spatial data in detecting the journey of living things over time. In health biology, the development of this science is also used to detect the spread of disease/pathogens from a region to the global direction. Phylogenetic analysis is able to describe the origin of the disease, the pattern of distribution, and speciation. In the pandemic analysis, several diseases that have been analyzed are the Chikungunya virus outbreak in 2007, Ebola and H3N2 influenza virus in 2014, and the SARS-CoV-2 virus in 2020. The study results reveal that the SARS-CoV-2 virus has three variants of lineage groups, namely types A, B, and C. The Covid-19 virus type A was first discovered in Wuhan City, China. Meanwhile, the type A virus mutation is seen in the United States and most of these viruses have also been found in corona positive patients from Australia. The Covid-19 type B virus was detected in patients living in East Asia, while the Covid-19 type was concentrated in the European region.
SELEKSI KETAHANAN ANGGREK TERHADAP Odontoglossum ringspot virus (ORSV) Irni Yuni Minarni; Mahfut Mahfut; Sri Wahyuningsih; Tundjung Tripeni Handayani
Teknosains Vol 15 No 2 (2021): Mei-Agustus
Publisher : Fakultas Sains dan Teknologi Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/teknosains.v15i2.20020

Abstract

Anggrek (Orchidaceae) merupakan salah satu tanaman hias yang digemari karena bentuk dan warna bunganya yang bervariasi serta dapat digunakan sebagai bunga potong, tanaman pot, atau elemen taman. Penyakit infeksi masih menjadi kendala utama dalam budidaya tanaman anggrek di Indonesia. Odontoglossum ringspot virus (ORSV) merupakan salah satu jenis virus yang paling banyak dilaporkan menginfeksi tanaman anggrek dan tersebar luas di dunia, termasuk di Indonesia. Infeksi menyebabkan perubahan warna dan bentuk daun serta bunga. Penelitian ini dilakukan dengan menginjeksi virus secara mekanis pada anggrek Phalaenopsis amabilis atau Dendrobium Salaya Fancy. Penelitian ini bertujuan untuk mengetahui gejala dan terjadinya penyakit, serta ketahanan anggrek terhadap ORSV. Hasil penelitian menunjukkan bahwa setiap anggrek memiliki gejala penyakit yang parah, tingkat keparahan penyakit pada kedua tanaman anggrek sekitar 80% serta reaksi Phalaenopsis amabilis sangat rentan dan respon Dendrobium Salaya Fancy toleran terhadap ORSV. Hal ini membuktikan bahwa Phalaenopsis merupakan jenis virus anggrek yang paling mudah terinfeksi dibandingkan Dendrobium.
Study of orchid resistance induced by Ceratorhiza sp. against ORSV infection based on peroxidase activity Anggi Anggreiny; Tundjung Tripeni Handayani; Mahfut Mahfut
Bioscience Vol 5, No 2 (2021): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202152112960-0-00

Abstract

Orchid is one of the popular ornamental plants that widely grown in Indonesia. However, the process of orchid cultivation is often hampered by virus infections. The virus that often infects the orchids is Odontoglossum ringspot virus. Viruses that enter orchid cells and replicate will activate the orchid’s defense response. This defense response is characterized by the increase of peroxidase activity. The peroxidase enzyme works in the formation of lignin to thicken cell walls and prevent viruses from entering other cells. In addition, the orchid defense response can also be activated through induced systemic resistance by inoculation of Ceratorhiza sp. as endophytic mycorrhizae. In this study, Factorial Complete Randomized Design (CRD) was used with 2 factors. Factor 1 is the type of orchid (Phalaenopsis amabilis and Dendrobium discolor) and factor 2 is the type of treatment (inoculation of mycorrhizae, virus, and mycorrhizae-virus). The orchid’s resistance level is determined by the analysis results of peroxidase activity using spectrophotometer. The results obtained indicate that all treatment combinations strongly influence the increase of peroxidase activity. Peroxidase activity of Phalaenopsis amabilis is 1.42 [(Umg) / min] and Dendrobium discolor is 1.64 [(Umg) / min] in average. Peroxidase activity on Dendrobium discolor was higher than on Phalaenopsis amabilis. This indicates that Dendrobium discolor has a higher level of resistance when compared to Phalaenopsis amabilis.Proses budidaya anggrek seringkali terkendala oleh infeksi virus. Adapun virus yang paling banyak menginfeksi anggrek adalah ORSV. Virus yang masuk ke dalam sel anggrek dan bereplikasi akan mengaktifkan respon pertahanan anggrek. Respon pertahanan ini ditandai dengan peningkatan aktivitas peroksidase. Respon pertahanan anggrek juga dapat diaktifkan melalui induksi resistensi sistemik dengan inokulasi Ceratorhiza sp. sebagai mikoriza endofit. Dalam penelitian ini digunakan Rancangan Acak Lengkap (RAL) Faktorial dengan 2 faktor. Faktor pertama adalah jenis anggrek (Phalaenopsis amabilis dan Dendrobium discolor) dan faktor kedua adalah jenis perlakuan (inokulasi mikoriza, virus, dan mikoriza virus). Tingkat ketahanan anggrek ditentukan dari hasil analisis aktivitas peroksidase menggunakan spektrofotometer. Hasil yang diperoleh menunjukkan bahwa semua kombinasi perlakuan sangat berpengaruh terhadap peningkatan aktivitas peroksidase. Aktivitas peroksidase Phalaenopsis amabilis rata-rata 1,42 [(U/mg)/ menit] dan Dendrobium discolor 1,64 [(U/mg)/ menit]. Aktivitas peroksidase pada Dendrobium discolor lebih tinggi dibandingkan dengan Phalaenopsis amabilis. Hal ini menunjukkan bahwa Dendrobium discolor memiliki tingkat ketahanan yang lebih tinggi jika dibandingkan dengan Phalaenopsis amabilis.
Chili Cultivation Technique Using Fermentation of Liquid Organic Fertilizer as Catfish Waste Utilization in Tasik Madu Village, Merbau Mataram Mahfut Mahfut; Yulianty Yulianty
Pelita Eksakta Vol 2 No 2 (2019): Pelita Eksakta Vol. 2 No. 2
Publisher : Fakultas MIPA Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/pelitaeksakta/vol2-iss2/71

Abstract

Cabai (Capsicum annuum L.) adalah tanaman yang termasuk ke dalam familia Solanaceae. Selain budidaya lele, budidaya tanaman cabai juga menjadi program produk unggulan anggota Kelompok Tani Sumber Rejeki II, Dusun Tasik Madu, Desa Talang Jawa, Kecamatan Merbau Mataram. Pemakaian pupuk oleh warga dalam budidaya tanaman cabai sebatas menggunakan pupuk kandang saat awal penanaman dan pupuk kimia sebagai tambahan. Informasi mengenai pembuatan pupuk organik cair hasil fermentasi menggunakan pemanfaatan limbah organik dari air kolam hasil budidaya lele konsumsi sangat bermanfaat untuk warga Kelompok Tani Sumber Rejeki II karena aplikasi pupuk cair organik tersebut sangat efektif dalam pertumbuhan tanaman cabai dan dapat menurunkan biaya produksi. Progam Pengabdian Kepada Masyarakat (PKM) DIPA BLU Yunior Universitas Lampung tahun 2017 ini dilakukan dalam tiga tahapan, yaitu; penyuluhan, pelatihan, dan pendampingan melalui kerjasama mitra dengan salah satu warga, Ketua Sumber Mulya Farm, Bapak Muslim. Hasil yang diperoleh dalam penyuluhan sebagai kegiatan awal adalah terjadi peningkatan pengetahuan peserta mengenai pembuatan pupuk organik cair hasil fermentasi limbah kolam lele. Hasil akhir program kegiatan PKM ini adalah aplikasi pemanfaatan limbah budidaya lele dalam pemeliharaan dan peningkatan produksi tanaman cabai.
Co-Authors Admi Syarif Alfina Amrani Alhuda Niftakul Ahyar Alir, Rahmat Fajrin Amanto Amanto Amnah, Alifah Zul Anbiya, Lulu Anggi Anggreiny Arie Setya Putra Armalia, Inayah Arsitalia, Metari Asadudin, David Asriah Nurdini M. Asrini Puspitasari Bambang Irawan Bambang Irawan /E21207042 Budi Setiadi Daryono Budi Setiadi Daryono BUDI SETIADI DARYONO Cut Muthiadin Della Apriyanti Derlian Ella Tamara Desti Deria Rahmadani Dian Alfiah Dwi Septiani Eka Nuraini Tohari Endah Susiyanti Endang Nurcahyani Endang Nurcahyani Eti Ernawati Eti Ernawiati Eti Ernawiati Eti Ernawiati Fadhilla, Nurul Fania Nur Izzati Fania Nur Izzati Febriansyah, Muhammad Fiska Humaizah Hajrah Hajrah, Hajrah Hasriadi Mat Akin Hasti Maulidya Fassya Heni Erlita Sari Heni Erlita Sari Heningtyas, Yunda Heri Satria Hermawan, Isna Ainun Insani, Mutiara Intan Poespita Windiyani Irni Yuni Minarni Ismail, Ikhwan Isna Rasdianah Aziz Kamisah Delilawati Pandiangan Kendari, Putri Khoirul Umur Kishy Dhea Herlanda Krisna Rendi Awalludin Kurnia Muludi Leni Agustin Lili Chrisnawati Lili Chrisnawati Lili Chrisnawati, Lili Lis Andriani Lulu Anbiya Lulut Dwi Sulistyaningsih M Kenedi M Said Hasibuan Mai Sari Mailinda Angraeni Marinda Sari Sofiyana Marjunus, Roniyus Maulidya Ananda Metari Arsitalia Mitha Valentina Treesya Panjaitan Mitha Valentina Treesya Panjaitan Mochammad Imron Awalludin Muh. Khalifah Mustami Muhammad Irfan Ardiansyah Muslimin Muslimin Nabela H.N. Harfiani Nur Qolbi, Hana Nurcayani, Endang Nurul Fadhilla Putri Kendari Rochmah Agustina Rochmah Agustrina Rochmah Agustrina RR. Ella Evrita Hestiandari Sahira Josy Arifannisa Sarah Sarah Sari, Heni Erlita Sari, Mai Sari, V. Dwi Anggita Sembiring, Rinawati Sijabat, Veronica Elizabeth Siti Zubaidah Sri Ratna Sulistiyanti Sri Wahyuningsih Sri Wahyuningsih Sri Wahyuningsih Sri Wahyuningsih Sri Wahyuningsih Suhara, . Sukimin Sukimin Sukimin Sukimin Sukimin Sukimin Suryafly, Fanny Dhea Susamto Somowiyarjo Susamto Somowiyarjo Susiyanti, Endah Tengku Riza Zarzani N Treesya Panjaitan, Mitha Valentina Tri Joko Tripeni Handayani, Tundjung Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani Tundjung Tripeni Handayani, Tundjung Tripeni Upreti, Brij Mohan Valentina Dwi Anggita Sari WAHYUDI Wandono, Petrus Tri Aji Widi Aryani WOLLY CANDRAMILA Yulia Rahma Syari Yulia Yulianty Yulianty Yulianty Yulianty Yulianty Yulianty Yulianty Yulianty Zelfi Julita Dwi Putri