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Bioeksperimen
Published by Universitas Muhammadiyah Surakarta
ISSN : 24601365     EISSN : 25272799     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Aim Bioeksperimen: Jurnal Penelitian Biologi is a peer-reviewed, open-access scientific journal dedicated to advancing knowledge across a broad spectrum of biological sciences through the publication of high-quality original research articles. The journal promotes rigorous experimental and exploratory research that advances fundamental and applied understanding in biological sciences. Bioeksperimen provides an international platform for researchers, academics, and practitioners to disseminate innovative empirical findings and scientifically robust investigations that contribute to the development of biological sciences at regional and global levels. The journal welcomes manuscripts that: 1. Present original experimental or exploratory research in biological sciences 2. Demonstrate strong methodological design and scientific rigor 3. Contribute novel data, findings, or biological insights 4. Address contemporary biological challenges and emerging scientific issues 5. Integrate laboratory, field, and computational approaches 6. Contribute meaningfully to the advancement of biological sciences All manuscripts must be original, unpublished, written in English, and must clearly demonstrate methodological soundness, data-driven analysis, and a significant contribution to biological sciences. Since March 2026, Bioeksperimen does not publish literature review articles, conceptual papers, or purely theoretical manuscripts. Focus The primary focus of Bioeksperimen: Jurnal Penelitian Biologi is on empirical biological research based on experimental, observational, or exploratory approaches. The journal prioritizes studies that: 1. Apply molecular, physiological, ecological, microbiological, or genetic techniques 2. Utilize omics-based and bioinformatics approaches to generate primary data 3. Conduct laboratory experiments, field investigations, or integrative biological exploration 4. Produce reproducible and evidence-based scientific findings Scope Bioeksperimen: Jurnal Penelitian Biologi publishes original research in the following areas: Main Topics: 1. Ecology and Environmental Sciences 2. Molecular Biology of Plants, Animals, and Microorganisms 3. Bioinformatics and Computational Biology 4. Omics-Based Studies (Genomics, Transcriptomics, Metagenomics, Proteomics, Metabolomics) 5. Basic and Applied Microbiology 6. Plant and Animal Physiology 7. Animal Behavior 8. Plant and Animal Systematics and Taxonomy 9. Genetics of Plants, Animals, and Microorganisms 10. Human Physiology
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 10 Documents
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Search results for , issue "vol. 12 no. 2: september 2026" : 10 Documents clear
Daily dynamics and structure of zooplankton communities as bioindicators in Merdada Lake, Dieng Plateau, Central Java, Indonesia Wahyu Febriani; Suwarno Hadisusanto
Bioeksperimen: Jurnal Penelitian Biologi Vol. 12 No. 2: September 2026
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v12i2.16113

Abstract

Prolonged anthropogenic disturbance contributes to the degradation of Merdada Lake. Tourism development, intensive water extraction, fertilizer and pesticide application, and the conversion of land into agricultural areas have accelerated soil erosion, sediment accumulation, nutrient loading, and siltation, which ultimately reduce water quality and influence zooplankton communities. This study assessed the suitability of zooplankton as a bioindicator by examining community composition, abundance, daily variation, ecological indices, and key environmental constraints as indicators of trophic conditions. Sampling was carried out randomly in May 2021 at five observation sites with three replicates, conducted during nighttime (7:00 PM), early morning (5:00 AM), and morning (9:00 AM). The zooplankton community consisted of several functional groups, including Cladocerans, Copepods, Nauplius, Protozoa, and Rotifers. Abundance ranged from 5.51 to 22.47 individuals per liter, with Rotifers, particularly Philodina sp., being the most dominant group. Peak abundance was recorded at night, reflecting diel movement patterns associated with vertical migration and food availability. Ecological index analysis revealed poor-moderate diversity (H’ = 1.76–1.90), moderate -high evenness (E = 0.72–0.80), and high dominance (C = 0.79–0.82), which indicates an unstable community structure and ecological. Variations in zooplankton structure and daily dynamics were strongly associated with light intensity, nitrate concentration, dissolved oxygen, and alkalinity. Overall, the findings demonstrate that zooplankton communities are sensitive to environmental changes and can serve as effective bioindicators of water quality in Merdada Lake. Overall, zooplankton communities are responsive to water quality conditions in Merdada Lake.
Effect of chicken feet collagen ointment (Gallus gallus domesticus L.) on incision wound healing in mice (Mus musculus L.) Zulfa Hafidzah; Rudy Agung Nugroho; Retno Aryani; Reni Kurniati; Eko Kusumawati
Bioeksperimen: Jurnal Penelitian Biologi Vol. 12 No. 2: September 2026
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v12i2.16402

Abstract

Chicken feet (Gallus gallus domesticus L.) are a by-product of a chicken slaughterhouse that has the potential to be a source of collagen for wound healing therapy. Collagen plays an important role in skin tissue regeneration, protein synthesis, and connective tissue formation during the healing process. This study aimed to characterize chicken feet collagen using Fourier Transform Infrared (FTIR), evaluate the physicochemical properties of collagen ointment preparations, and analyze their effect on the percentage of incision wound closure, protein levels, and hydroxyproline in mice (Mus musculus L.). The study was conducted as a laboratory experiment with a complete randomized design, including negative controls, two positive controls, and three groups of collagen ointment at concentrations of 5%, 10%, and 15%. Collagen is extracted using the Acid Soluble Collagen (ASC) method and is formulated in the form of an ointment. FTIR characterization shows the presence of amide groups A, B, I, II, and III as characteristic of collagen. The ointment preparation meets physical and chemical requirements, including homogeneity, semi-solid consistency, and pH according to the skin. Collagen ointment increased the percentage of incision wound closure across all groups without a significant difference among treatments. Tissue protein levels declined progressively from the untreated control through the treated groups, reaching the lowest value at the 5% concentration (P1). Hydroxyproline levels, by contrast, did not follow a uniform pattern: the 5% ointment (P1) showed hydroxyproline levels comparable to the positive controls, whereas the 10% and 15% ointments (P2, P3) showed hydroxyproline levels comparable to the untreated negative control, suggesting that healing in the P2 and P3 groups remained in an active, collagen-synthesizing phase at the time of sampling. These results suggest that chicken feet collagen has the potential to be an active ingredient in topical preparations to support the healing of incision wounds.
Effect of topical collagen extract ointment from Oreochromis niloticus L. scales on incision wound healing in Mus musculus L. Liliana Devita Sari; Rudy Agung Nugroho; Retno Aryani; Reni Kurniati; Eko Kusumawati
Bioeksperimen: Jurnal Penelitian Biologi Vol. 12 No. 2: September 2026
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v12i2.16418

Abstract

Nile tilapia (Oreochromis niloticus L.) ranks among Indonesia’s most heavily farmed freshwater fish, and the scales discarded during its processing are a rich, underused source of type I collagen, a structural protein of the extracellular matrix that supports skin repair and could serve as a natural ingredient in wound-care ointments. This study evaluated the physical properties of an ointment formulated with tilapia scale collagen extract and examined its effect on incision wound healing in mice (Mus musculus L.). Six groups were compared: K- (untreated), K+1 (bioplacenton), K+2 (ointment base only), and three collagen-ointment concentrations, P1 (5%), P2 (10%), and P3 (15%). Collagen was isolated by the acid-soluble collagen (ASC) method and characterized by Fourier-transform infrared (FTIR) spectroscopy; wound closure, tissue protein content (Lowry method), and hydroxyproline content were assessed over 14 days. All three ointment formulations met the general pharmaceutical standards for topical preparations. Wounds in every group closed gradually, reaching a mean closure of 87.4-98.2% by day 14 without a statistically significant difference among treatments, whereas protein and hydroxyproline assays showed that the 15% collagen ointment produced the lowest tissue protein (397.0 ± 8.55 μg/mL) and hydroxyproline (82.05 ± 4.45 μg/mL) levels, consistent with an accelerated transition into the tissue remodeling phase.
Prediction of hemoglobin protein structure and function of the α4.2 deletion mutation and αα/-α4.2/βCD8 (-AA)/βN co-inheritance Nurul Fitri T Tagunu; Tri Ratnaningsih; Nur Imma Fatimah Harahap; Indra Lesmana; Niken Satuti Nur Handayani
Bioeksperimen: Jurnal Penelitian Biologi Vol. 12 No. 2: September 2026
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v12i2.16912

Abstract

The α4.2 deletion is typically characterized as a silent mutation. The clinical impact of this mutation can vary, particularly when inherited alongside other globin gene mutations, for example co-inheritance with β-thalassemia mutations, such as βCD8 (-AA)/βᴺ. This study aims to determine the α4.2 deletion breakpoint and analyze the structural and functional impact of α-thalassemia heterozygous mutation associated with α4.2 deletion and co-inheritance of αα / -α4.2/ βCD8 (-AA) / βN on the resulting hemoglobin protein. DNA sample was sequenced using the Sanger method by 1st BASE laboratory. Sequence alignment was performed via Clustal Omega, while hemoglobin structure prediction and analysis were conducted using UCSF Chimera (Chimera-1.19) and BIOVIA Discovery Studio. The results identified the α4.2 deletion breakpoint between nucleotide 26 of the X2 box and nucleotide 347 of the X1 box. Despite the mutations, the α4.2 deletion and the αα / -α4.2/ βCD8 (-AA) / βN co-inheritance resulted in a protein structure identical to normal residues, maintaining all stabilizing interactions. Therefore, we conclude that in silico predictions indicate no major structural deviations from wild-type hemoglobin A for both the α4.2 deletion and the co-inheritance of αα/-α4.2 with βCD8 (-AA)/βN. This is consistent with the molecular nature of α-thalassemia deletions, which primarily affect globin gene dosage rather than amino acid sequence, although this observation is limited to computational analysis and has not been experimentally confirmed.
The effect of coconut water on the in vitro growth of Phalaenopsis amabilis (L.) Blume plantlets Elvina Adinda Triansyah; Nintya Setiari; Yulita Nurchayati
Bioeksperimen: Jurnal Penelitian Biologi Vol. 12 No. 2: September 2026
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v12i2.16917

Abstract

Phalaenopsis amabilis is an Indonesian native orchid listed in Appendix II, therefore, appropriate cultivation strategies, including tissue culture techniques, are required to support its sustainable propagation. However, Murashige and Skoog (MS) medium does not contain sufficient natural growth-promoting substances; thus, organic supplementation such as coconut water is needed to enhance plantlet development. This study aimed to evaluate the effect of coconut water supplementation in MS medium and to determine the optimal concentration for promoting the growth of P. amabilis plantlets during the subculture stage. Plantlets were subcultured onto MS medium supplemented with coconut water at concentrations of 0%, 15%, and 30% using a Completely Randomized Design with five replications. Data were analyzed using ANOVA followed by Duncan’s Multiple Range Test at a 5% significance level. Observations were conducted for two months on leaf emergence time, leaf length and width, number of leaves, shoot emergence time, number of shoots, root emergence time, number of roots, and leaf color. The results showed that 15% coconut water was the optimal concentration for improving plantlet growth, particularly in new leaf length (8 mm), number of shoots (1,66 shoots), and number of leaves (1,33 leaves). whereas 30% coconut water increased leaf width (3,2 mm). Overall, supplementation with 15% coconut water effectively enhanced the vegetative growth of P. amabilis plantlets, so it can be applied to successfully cultivate this plant.
Colonization of Streptomyces enissocaesilis on the root system of Zea mays var ceratina Velma Agtavia; Yuliana Retnowati; Novri Youla Kandowangko; Jusna Ahmad; Abubakar Sidik Katili
Bioeksperimen: Jurnal Penelitian Biologi Vol. 12 No. 2: September 2026
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v12i2.17484

Abstract

Streptomyces enissocaesilis is a microorganism with diverse biological activities; it is capable of colonizing plant root systems by forming mycelium and adhering to root surfaces.. This study aimed to analyze the colonization of Streptomyces enissocaesilis in the root system of Zea mays var ceratina based on root infection ability, metabolite profiles in growth media, and plant vegetative growth. Zea mays var ceratina was grown hydroponically using the Deep Flow Technique (DFT) for 30 days with inoculation (1.1 × 10⁸ CFU/g) and without inoculation of Streptomyces enissocaesilis as a control. Plant growth was monitored at 7-day intervals, while infectivity and metabolite profiles were assessed at the end of the growth period; metabolite screening in the growth medium was performed using LC-MS.The results showed that Streptomyces enissocaesilis was able to infect plant root tissue, as well as interact on the root surface, as indicated by filamentous structures resembling hyphae on the epidermis of treated plant roots. Analysis of the metabolite profile in growth media inoculated with Streptomyces enissocaesilis using LC-MS revealed peaks detected at retention times of 0.72 and 1.18 minutes. Inoculation of Streptomyces enissocaesilis during the vegetative growth of Zea mays var ceratina, based on plant height and root length data, showed significantly higher values compared to the control; however, no significant differences were observed for the parameters of leaf number, dry weight, and root-to-shoot ratio. Based on these data, inoculation with Streptomyces enissocaesilis has the potential to function as a Plant Growth Promoting Rhizobacteria (PGPR). Based on the research findings, Streptomyces enissocaesilis has the potential to be developed as a plant growth promoter. However, its effectiveness in increasing biomass and overall plant growth still requires further study.
Altitudinal morphometric variation of Polypedates leucomystax (Gravenhorst, 1829) in Yogyakarta, Indonesia Fredo Meical Cibro; Agung Budiantoro
Bioeksperimen: Jurnal Penelitian Biologi Vol. 12 No. 2: September 2026
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v12i2.17538

Abstract

Indonesia is one of the world's megabiodiverse countries due to its tropical climate and diverse geographic landscape. One of the species widely distributed in Indonesia is Polypedates leucomystax. Imogiri Nature Reserve and Merapi Valley represent two locations with contrasting altitudes, which ultimately drive adaptive responses and cause morphometric variation among P. leucomystax populations inhabiting the two areas. The aim of this study was to analyze the morphometric variations of P. leucomystax frogs in the Imogiri Nature Reserve and Merapi Valley and also to analyze the relationship between abiotic factors and differentiated morphometric characters. Morphometric character data were tested using the Mann-Whitney test (p≤0.05), followed by the Spearman correlation test was conducted with abiotic factors. The morphometric characters that differed significantly in males were STL, NS, EN, MBE, THIGHL, and MTI, whereas in females the significant characters were NS, MFE, MBE, IOD, UEW, and 1TL. In addition, temperature, humidity and altitude also have a correlation with the differentiated characters. In male frogs, altitude correlated with the characters EN, MBE, THIGHL, MTI and temperature correlated with MBE, THIGHL, MTI, while in female frogs altitude correlated with the characters NS, MFE, MBE, IOD, UEW, 1TL and temperature correlated with NS, MFE, MBE, IOD. Then humidity correlated with UEW. The conclusion of this study is that there are 6 variations in morphometric characters in male frogs and 6 characters in female frogs found in Merapi Valley and the Imogiri Nature Reserve. Furthermore, abiotic factors are also associated with differentiated morphometric traits.
Parasitoid hymenoptera community in cayenne pepper (Capsicum frutescens L.) and peanut (Arachis hypogaea L.) intercropping systems Amrul Jihadi; Abdul Halim Mursyidin; Bambang Supeno; Muhammad Sarjan; Dwi Noorma Putri; Anjar Pranggawan Azhari
Bioeksperimen: Jurnal Penelitian Biologi Vol. 12 No. 2: September 2026
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v12i2.17542

Abstract

Intercropping systems, an ecological engineering strategy, have the potential to increase the abundance of parasitoid Hymenoptera in agroecosystems. This study aimed to analyze the diversity, relative abundance, and composition of parasitoid Hymenoptera in different intercropping systems of cayenne pepper and peanuts, based on differences in the peanut plant composition. The study was conducted using a randomized block design (RBD) with four treatments and five replicates. Sampling was performed using yellow pan traps and pitfall traps. Data were analyzed using the Shannon-Wiener diversity index (H'), evenness (E), dominance (D), and species richness (R). The results show that the parasitoid Hymenoptera community consisted of 79 individuals belonging to 12 families and 29 morphospecies. The highest relative abundance was found in Trichopria spp. (24 individuals; 30.37%). The Diapriidae family was the most abundant, with 33 individuals (41.77%). The TS2 treatment (ratio 1:3) resulted in the highest diversity (H' = 2.36) and species richness (R = 4.84). However, ANOVA indicated that neither parasitoid abundance (F3,12 = 2.599; P > 0.05) nor morphospecies richness (F3,12 = 3.207; P > 0.05) differed significantly among treatments. These findings suggest that cayenne pepper–peanut intercropping may support parasitoid communities, although the effects of different peanut proportions require further investigation under broader ecological conditions.
Water quality assessment and characterization of pathogenic Escherichia coli in the Bangkahulu River, Bengkulu, Indonesia Rangga Ardiansyah; Risky Hadi Wibowo; Abdi Wira Septama; Sipriyadi; Enny Nugraheni; Rudy Hermawan
Bioeksperimen: Jurnal Penelitian Biologi Vol. 12 No. 2: September 2026
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v12i2.18135

Abstract

The Bangkahulu River is one of the main rivers that is very important in Bengkulu Province, which is currently facing significant human-induced pressures. This study aims to evaluate the water quality of the Bangkahulu River, as well as to isolate, characterize, and profile the hemolytic Escherichia coli isolated from the upstream to downstream areas. Water samples were taken from five stations using a purposive sampling method. Physical, chemical, and microbiological parameters were analyzed according to the Indonesian national standards (SNI), while E. coli was isolated and identified through morphological characterization, Gram staining, and biochemical tests. Hemolytic activity of the isolates was tested on blood agar. The results showed that Biological Oxygen Demand (BOD), Chemical Oxygen Demand (COD), Total Suspended Solids (TSS), and Total Dissolved Solids (TDS) exceeded the Class I water quality standards as regulated by Government Regulation Number 22 of 2021. The Pollution Index values range from 2.15 to 6.32, indicating a light to moderate level of contamination. Out of 35 identified E. coli isolates, 18 showed ß-hemolytic activity, 12 showed γ-hemolytic activity, and 2 had hemolytic activity. The prevalence of ß-hemolytic isolates suggests the presence of potentially active phenotypic strains in the aquatic environment. The dominance of β-hemolytic isolates points to strains with phenotypic traits related to potential virulence, although hemolytic activity alone is not enough to determine pathogenicity. This finding provides basic information about the physical and chemical pollution levels of the Bangkahulu River and serves as a foundation for further knowledge and research on the importance of continuously monitoring water quality and implementing effective pollution control strategies to reduce potential public health risks from microbial contamination and physical and chemical pollution, ensuring the sustainable use of river water resources.
Contribution of riparian vegetation to offset transportation-derived CO₂ emissions Ilham Ikhlasul Amal; Eka Sulistiyowati
Bioeksperimen: Jurnal Penelitian Biologi Vol. 12 No. 2: September 2026
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v12i2.19482

Abstract

Riparian vegetation has high potential for carbon absorption and storage. The study aimed at exploring thestudy the potential of riparian vegetation in reducing carbon from the transportation sector. The study was conducted at Boyong River and its triburatory Trasi River from February - March 2026. Location selection was carried out purposively in open and closed canopy areas. Vegetation data were collected using square belt plots, CO2 emissions estimated with traffic counts, and carbon storage estimated with allometric equations (trees) and destructive sampling (herbs). Data were analyzed to obtain IVI, H’, and E indices, and the correlations among variables using Canonical Correspondence Analysis ( CCA).The results show that closed areas are dominated by trees, particularly Cocos nucifera, Leucaena leucocephala, and Swietenia macrophylla. Open areas are dominated by herb, especially Mimosa pudica, Alternanthera philoxeroides, and Typhonium blumei. Transportation emissions were higher on weekends then weekdays. The percentage of CO2 absorption in closed areas (21.65% and 27.95%) was higher than in open areas (2.01% and 16.12%). Carbon storage in trees was greater in closed areas, while herbaceous carbon storage was greater in open areas. The CCA analysis indicated that closed areas are associated with the stability of tree and shrub communities, higher tree carbon storage, and lower temperatures. Meanwhile, open areas are associated with herb community stability, higher herb carbon storage, and higher temperatures. In conclusion, closed areas have greater support for carbon absorption and storage then open areas.

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