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FORMULASI EKSTRAK ETANOL DAUN MALAKA (Phylanthus emblica) DALAM SEDIAAN MASKER GEL PEEL-OFF Agustina Bayo Inggirik Mogan; Ni Luh Arpiwi; Ni Nyoman Wirasiti
SIMBIOSIS Simbiosis Volume 12 No. 1 2024
Publisher : Program Studi Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2024.v12.i01.p01

The use of facial masks is useful for softening the skin, nourishing and opening clogged pores, helping prevent premature aging, and reducing the appearance of fine lines and wrinkles. Plants that can be used as active ingredients are malaka, or amla (Phyllanthus emblica L.), a natural source of antioxidants. This study aims to determine the content of phytochemical compounds in the ethanol extract of Malaka leaves, to analyze the antioxidant activity and IC50 of the ethanol extract of Malaka leaves, to determine the quality of the peel-off gel mask with the addition of the concentration of the ethanol extract of Malaka leaves and to find out which formulation is preferred by Probandus. The research design used a completely randomized design (CRD) using Malaka leaf extract with concentrations F0(0%), F1(1%), F2(2%) and F3(3%). The phytochemical content test of Malaka leaf extract showed the presence of flavonoids (0.467%) and tannins (10.97%). The antioxidant activity of the ethanol extract of Malaka leaves was 1.3314% and IC50 was 30 ppm. The organoleptic test results for all preparations did not change during 5 weeks of storage, pH 5.37-5.54, the viscosity of the mask preparation was 50000-126000 cps, the peel-off gel mask preparation was homogeneous and did not . Statistical analysis of the

KEANEKARAGAMAN CAPUNG (ORDO: ODONATA) DI KAWASAN WISATA SEMARA RATIH DESA TARO, GIANYAR-BALI Tresia Uliani Purba; Ni Made Suartini; Ni Luh Watiniasih
SIMBIOSIS Simbiosis Volume 12 No. 1 2024
Publisher : Program Studi Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2024.v12.i01.p02

Dragonfly is an insect with an interesting morphology and is often found in areas that are still beautiful and unpolluted, making it an environmental bioindicator.. This research was conducted to determine the diversity of dragonfly species and environmental factors that could influence their existence. The study was carried out in January-February 2023 using the exploratory method. Samples were taken from three station points in the morning and evening five times. The captured dragonfly samples were then identified by observing their morphological characters such as body shape, color pattern on the thorax and abdomen, wing venation, and wing color pattern. Environmental factors of temperature, humidity and altitude where measured every sampling site. The results obtain ten species of dragonflies were found, namely Copera marginipes, Agriocnemis pygmaea, Agriocnemis femina, Ischnura senegalensis, Orthetrum testaceum, Orthetrum sabina, Potamarcha congener, Neurothemis ramburii, Neurothemis terminata. The diversity index obtained was in the medium category with almost even distribution and no dominating species were found. Environmental conditions support the existence of dragonflies in Semara Ratih, Taro Tourism Village, Gianyar-Bali so that in the future it can be used as a tourist attraction in the area. Keywords: dragonfly, identification, morphological characters, habitat, Taro

ISOLASI DAN IDENTIFIKASI JAMUR ENDOFIT PADA TANAMAN STROBERI (Fragaria × ananassa Duch. ‘Rosalinda’) DI DESA CANDIKUNING KABUPATEN TABANAN, BALI Meniwati; Anak Agung Darmadi; Junita Hardini
SIMBIOSIS Simbiosis Volume 12 No. 1 2024
Publisher : Program Studi Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2024.v12.i01.p03

Strawberries (Fragaria × ananassa Duch.) are subtropical plants well adapted to Indonesia’s highlands. Strawberries cultivated in Candikuning Village, Tabanan Regency, Bali are Fragaria × ananassa Duch. 'Rosalinda'. The fruit is widely used because it produces bioactive compounds. Bioactive compounds can also be obtained from endophytic fungi that live in host tissues. The study aimsed to identify the genus of endophytic fungi on the leaves and petioles of strawberries and to find out the benefits of the endophytic fungi found (literature review). This study used a descriptive method, which was carried out from December 2022 to February 2023. The stages in this study were the isolation of endophytic fungi, purification, and macroscopic and microscopic identification. There were 10 isolates of endophytic fungi isolated from leaves and petioles. Nine isolates were identified Aspergillus, Cercospora, Alternaria, Geotrichum, Penicillium, Cladosporium, Cylindrocladium, Cunninghamella, and Phytophthora. One isolate was not identified (mycelia sterile). These endophytic fungi are useful as biological controllers against plant pathogens, medicinal ingredients, and insecticides. Keywords: Candikuning, endophytic fungi, identification, isolation, strawberries

KUALITAS SPERMATOZOA DAN BERAT ORGAN REPRODUKSI MENCIT (Mus musculus) SETELAH PEMBERIAN EKSTRAK BIJI KLABET (Trigonella foenum-graecum L.) Maulidya Rosa Putri Utama; Dwi Ariani Yulihastuti; Sang Ketut Sudirga
SIMBIOSIS Simbiosis Volume 12 No. 1 2024
Publisher : Program Studi Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2024.v12.i01.p04

Indonesia is one of the countries known to have the highest biodiversity in the world. There are 18 types of herbs that have the potential as antifertility in men, one of which is the klabet plant or fenugreek. Klabet is believed by ordinary people to have properties to increase the production and quality of spermatozoa. According to previous research klabet has toxic effects that can inhibit fertility due to the content of phytochemicals in it. This study aims to determine the effect of fenugreek seed extract on the quality of spermatozoa and the weight of the reproductive organs of mice. The mice used were 20 male mice weighing about 25-30 grams divided into 4 treatments namely control (P0), 56 mg/kgBB (P1), 112 mg/kgBB (P2), 168 mg/kgBB (P3) with 5 repetitions. Giving treatment for 24 days. The method used is RAL (Complete Randomized Design) and the resulting data is analyzed using ANOVA. The parameters studied were testicular weight, epididymis weight, motility, morphology and number of male mouse spermatozoa. The results showed that there was a real difference (p≤0.05) between the control and treatment of the klabet seed extract on the quality of spermatozoa and the weight of the testicle but not significantly different (p≥0.05) to the weight of the epididyimis. Concentration of mice spermatozoa decreased by 10.60%, progressive spermatozoa motility decreased by 55.61%, nomal morphology decreased by 57.92%, while testicular weight decreased by 38.46%. The most effective dose of fenugreek seed extract used as an antifertility agent was a dose of 168 mg/kgBW (P3).

INTERPRETASI EKOWISATA OLEH PEMANDU WISATA DI KAWASAN PARIWISATA UBUD Ashira Istighfarah; Anak Agung Gde Dalem; I Ketut Ginantra
SIMBIOSIS Simbiosis Volume 12 No. 1 2024
Publisher : Program Studi Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2024.v12.i01.p05

Tourism is one sector whose development which very intensively developed by the government. One form of sustainable tourism is ecotourism. Tour guides in ecotourism activities have an important role in connecting tourists with interpreted tourist attractions. Interpretation is a tool for connecting what tourists should get in an environment with what should be displayed. Interpretation is very important in educating tourists so they understand, respect, and support nature conservation, as well as provide satisfaction to tourists. Therefore, research on the interpretation of ecotourism is very important for tourism in the Ubud tourism resort. This research was conducted from January 17- May 3, 2023. The objective of this study is to determine the type of interpretation, the means of interpretation and the quality of the interpretation of tour guides. The methods used were observation, interviews, and questionnaires distribution. The interviewed guides were 16 informants and 30 tourists as respondents. This research found that there were 8 types of interpretation utilised by tour guides in the Ubud tourism resort: guided tour, point duty, roving interpretation, presentation, demonstrations, living history, interactive, and lecture or talk. There are 8 types of facilities used by guides in the Ubud tourism resort, print, self-guiding program signs, interpretive panels, visitor center, video, slideshow, observation hides, and handphone. The interpretation quality of the tour guides in the Ubud tourism resort was very good with a score of 92.21 %. Tourist satisfaction with the tour guide and the interpretation given by the tour guide was very good with a score of 85.16%. Keywords: Attractiveness, Bali, Tourist Destinations, Tourists, Villages

MORFOLOGI DAN TIPE STOMATA DELAPAN SPESIES BAMBU KOLEKSI KEBUN RAYA EKA KARYA BALI Faizun Nadhiyah Nitawaro; Made Pharmawati; Ni Made Gari; Arief Priyadi
SIMBIOSIS Simbiosis Volume 12 No. 1 2024
Publisher : Program Studi Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2024.v12.i01.p06

Eka Karya Bali Botanic Garden is one of the ex-situ plant conservation centers in the island of Bali. There are various collections of plants, one of which is bamboo. Indonesia is estimated to have 176 species of bamboo which is more than 12% of the world's bamboo species. The Eka Karya Bali Botanical Garden has a collection of 58 bamboo collections. Several genera among the existing bamboo collections, namely Bambusa, Gigantochloa, Schizotachyum and Neololeba need to be characterized morphologically. This study aimed to analyze the morphological and types of stomata on eight bamboo species (Neololeba atra, Bambusa maculata, Bambusa ooh, Giganthochloa apus, Giganthochloa aya, Giganthochloa taluh, Schizostachyum lima, dan Schizostachyum silicatum) from the “Eka Karya” Botanical Garden collection. The morphological characters observed were bamboo clumps, stems color, branches, reed sheaths color, and leaves. For observation of stomatal characteristics, the type and density of stomata were observed by making a replica of the lower epidermis of the leaf and observing it under a microscope with a magnification of 400x. The results showed that the morphological characters of the eight bamboo species had dense clumps and lateral branches but there were differences in the color of the stems, sheath, upper and lower surfaces of the leaves, while the type of stomata was the same, namely the type Gramineae. Keywords: Bamboo, stem, leaf, morphology, stomata

LAMA WAKTU PENGHENTIAN KONSUMSI SOJU TERHADAP KADAR ALKOHOL HATI, MORFOLOGI HATI, DAN GAMBARAN HEMATOLOGI TIKUS PUTIH Tiffany Angelita Putri Mileva; Ni Wayan Sudatri; I Made Sara Wijana
SIMBIOSIS Simbiosis Volume 12 No. 1 2024
Publisher : Program Studi Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2024.v12.i01.p07

Alcohol consumption behaviors such as soju can increase metabolic syndrome disease. The elimination of alcohol in the body can determine the last time a person consumed alcohol through post-absorption assessment of alcohol. This study was conducted to determine changes in liver macroscopic morphology, liver alcohol levels and hematological features after stopping soju administration in white rats. The research design used was a complete randomized design with 4 treatments, namely the examination time P(0) 0 days, P(1) 3 days, P(2) 7 days, and P(3) 10 days. The methods used in this study are liver weight weighing, observation of macroscopic morphological conditions of the liver, examination of alcohol levels with gas chromatography-flame ionization detector and hematological examination with haematology analyzer. The results showed that stopping soju for 10 days had a significant effect on reducing liver weight and reducing fatty liver, decreased alcohol levels, red blood cell count, PCV percentage and hemoglobin levels that returned to the normal range. While the number of white blood cells does not show any influence and the value is below the normal range. Keywords: alcohol, liver, blood profile, soju, white rat

DETEKSI DAN IDENTIFIKASI PATOGEN JAMUR TERBAWA BENIH PADA MALAPARI [Pongamia pinnata (L.) Pierre] Salma ‘Ariqotul Faridah ML; Ni Luh Arpiwi
SIMBIOSIS Simbiosis Volume 12 No. 1 2024
Publisher : Program Studi Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2024.v12.i01.p08

The existence of malapari as a potential bioenergy plant relies on the natural populations in coastal forests, which are decreasing due to erosion and human activities. The provision of high-quality malapari seedlings, mostly through generative reproduction, is necessary to enhance its population in nature. However, one of the obstacles encountered during the seed germination process is the low germination capacity or ability of seeds due to poor seed quality. The presence of seed-borne pathogens affects seed health. This study aims to identify seed-borne pathogenic fungi and seed germination capacity in three malapari accessions. The research was carried out at the Plant Taxonomy (Mycology) Laboratory, Biology Program, Faculty of Mathematics and Natural Sciences, Udayana University, from January to February 2023, using malapari seeds obtained from three accessions, namely Tangguwisia, Umeanyar, and Seririt. The seeds were sterilized, followed by the blotter test method and direct sowing method on Potato Dextrose Agar media, then incubated at room temperature. Isolates that grew were reisolated on fresh PDA media to obtain pure cultures for subsequent observation and identification at the genus and/or species level. The results revealed the presence of Aspergillus flavus, Aspergillus niger, Rhizoctonia solani, Penicillium sp., Fusarium sp., Colletotrichum sp., and Alternaria sp. The percentage of germination capacity was highest in Seririt (38%), followed by Umeanyar (35%), and Tangguwisia (31%). Keywords: Aspergillus, blotter test, fabaceae, germination percentage, viability

KUALITAS AIR SERTA PERILAKU MASYARAKAT DALAM MENGELOLA LIMBAH PADAT DAN AIR LIMBAH DI SUNGAI AYUNG, KELURAHAN KESIMAN PETILAN KOTA DENPASAR I G H Purnama; M A H Suryadhi Suryadhi; N L M A S S B Rahmi
SIMBIOSIS Simbiosis Volume 12 No. 1 2024
Publisher : Program Studi Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2024.v12.i01.p09

Based on the development plan for Kesiman Petilan Village, Ayung River, starting from the Bridge on Jalan Sulatri to the Balitex Bridge on Jalan WR. Supratman – Denpasar City, will be arranged into a new water tourism spot. This new tourist location will contain water sports, restaurants, fishing spots and so on. The study aims to analyze the health risks of the bacteriological quality of water passing through the two segments of the Ayung River, as well as to examine the content of heavy metals Lead (Pb), Cadmium (Cd), in sediments and fish caught in the river, as well as to determine the behavior of the surrounding community in managing solid waste and waste water. The study uses a quantitative descriptive design. The results showed that overall, the content of Coliform and Escherichia coli in the Ayung River segment was above 10,000 MPN/100mL, far exceeding the quality standard allowed in Bali Governor Regulation Number 16 of 2016 concerning Environmental Quality Standards and Environmental Pollution Standard Criteria, especially in water, for water recreation tourism in Class II, which is 1000MPN/100mL, so that the water of the Ayung River is bacteriologically polluted. Likewise, the heavy metal content in river sediments exceeds the permissible standard. The content of heavy metals in caught fish and shrimp is still below the Indonesian National Standard (SNI) 7387 of 2009 concerning the maximum limit of heavy metal contamination in food. In addition, people's behavior in disposing of solid waste and household waste water around the Ayung River still needs to be improved so as not to pollute the environment and the Ayung River. Therefore, efforts are needed to further deepen public knowledge and awareness in preserving the Ayung River. Keywords: bacteriological, heavy metal, garbage, liquid waste, behavior, Ayung River

AKTIVITAS TEH (AIR SEDUHAN ) DAUN KELOR SEBAGAI HEPATOPROTEKTOR PADA TIKUS PUTIH JANTAN YANG DIINDUKSI FORMALIN DOSIS TOKSIK Ida Bagus Suaskara; Ni Gusti Ayu Ermayanti; Martin Joni; I Ketut Ginantra; Anastesya .
SIMBIOSIS Simbiosis Volume 12 No. 1 2024
Publisher : Program Studi Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2024.v12.i01.p10

An attempt to avoid formalin side effects of toxin dose contained in food is to administer hepatoprotectant. Moringa is one of the food sources that functions as a hepatoprotector. The purpose of this study was to determine the activity of Moringa leaf tea (steeping water) as an antioxidant and hepatoprotector in male white rats induced with formalin doses of toxicity. The method used in this study was a randomized group design (RGD). The liver damage barrier test used a formalin-induced male white rat model (0.100 ml/BB) as a negative control, tea (steeping water) of Moringa leaves was given at a dose of 50, 75, 100 mg/BB once a day and the administration of aquades as a positive control for 7 days. The formalin-induced group of mice showed a significant increase in SGPT, SGOT and MDA values compared to positive controls. Orally administering Moringa leaf tea (steeping water) can weaken or decrease the increase in SGPT, SGOT and MDA values in male rats compared to formalin-induced significantly. So that giving tea from steeping water Moringa leaves can be an alternative as drinking water after meals. Keywords: toxic, SGOT, SGPT, MDA

Home Page

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Editorial Team

Contact

Reviewer

Google Scholar

Contact Name
Ni Wayan Sudatri

Contact Email
wayan_sudatri@unud.ac.id

Phone
+6281805550907

Journal Mail Official
simbiosisbiologi@unud.ac.id

Editorial Address
Jln Pratu Made Rampug Gg Mekarsari 14 Batubulan, Gianyar, Bali

Location
Kota denpasar,

Bali

INDONESIA

Abstract

Abstract

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Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Ni Wayan Sudatri

Contact Email wayan_sudatri@unud.ac.id

Phone +6281805550907

Journal Mail Official simbiosisbiologi@unud.ac.id

Editorial Address Jln Pratu Made Rampug Gg Mekarsari 14 Batubulan, Gianyar, Bali

Location Kota denpasar, Bali INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Ni Wayan Sudatri

Contact Email
wayan_sudatri@unud.ac.id

Phone
+6281805550907

Journal Mail Official
simbiosisbiologi@unud.ac.id

Editorial Address
Jln Pratu Made Rampug Gg Mekarsari 14 Batubulan, Gianyar, Bali

Location
Kota denpasar,

Bali

INDONESIA