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All Journal Buletin Udayana Mengabdi Bumi Lestari Jurnal Bios Logos Biosaintifika: Journal of Biology & Biology Education Jurnal Widya Biologi SIMBIOSIS
Ni Nyoman Wirasiti
Program Studi Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Udayana
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Energy Environmental Science Immunology & microbiology Medicine & Pharmacology Public Health

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Respon Morfologis dan Ekspresi Gen Aquaporin pada Padi IR 64 yang Mengalami Cekaman Kekeringan pada Fase Reproduktif (Morphological Responses and Aquaporin Gene Expression in Rice IR64 under Drought Stress at the Reproductive Stage) Pharmawati, Made; Wirasiti, Ni Nyoman; Wrasiati, Luh Putu
JURNAL BIOS LOGOS Vol 7, No 2 (2017): JURNAL BIOSLOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.7.2.2017.18579

Abstract

Abstrak Cekaman kekeringan merupakan faktor pembatas penting bagi pertumbuhan dan produktivitas tanaman termasuk padi.      Penelitian ini bertujuan menganalisis respon padi IR64 terhadap cekaman kekeringan dengan pemberian polietilen glikol (PEG) pada fase reproduktif.  Penelitian juga bertujuan menganalisis ekspresi gen aquaporin akibat cekaman kekeringan.  Bibit padi ditanam dalam pot dan perlakuan PEG dengan konsentrasi 108g/L (-0.25MPa) dan 178g/L (-0.52 MPa) diberikan saat munculnya panikula. Perlakuan diberikan selama 2 minggu, kemudian tanaman disiram kembali.  Ekspresi gen diamati pada akhir perlakuan dengan semi kuantitatif real time PCR.  Ekstraksi RNA menggunakan RNeasy plant mini kit, sedangkan sintesis cDNA menggunakan Transcriptor First Strand cDNA Kit.  Hasil penelitian menunjukkan bahwa jumlah malai dan berat total malai berkurang akibat cekaman kekeringan.  Persentase gabah kosong mencapai 84,6% pada perlakuan PEG-0,52 MPa, sedangkan pada perlakuan PEG -0,25 MPa persentase gabah kosong sebesar 67,8%.  Pada kontrol persentase gabah kosong adalah 10,3%.  Ekspresi gen OsPIP2;7 sedikit menurun pada perlakuan PEG -0,52 MPa.Kata kunci: ekspresi gen, IR64, kekeringan, padi, PEG  Abstract Drought stress is one of the limiting factors of plant growth and productivity including rice.  The aim of this study was to analyze responses of IR64 rice to polyethylene glycol (PEG)-induced-drought stress at the reproductive stage.  This study also aimed to analyze the expression of aquaporin under drought stress.  Rice seedlings were grown in pot system and PEG treatment at concentration of -0.25MPa (108g/L) and -0.52 MPa (178g/L) were given when the panicles arose.  Treatments were conducted for 2 weeks, after that the plants were rewatered.  Gene expression was evaluated at the end of PEG treatment using semi quantitative real time PCR. RNA was extracted using RNeasy plant mini kit, while cDNA synthesis was done using Transcriptor First Strand cDNA Kit.  The results showed that the number and weight of rice ear were less in plant treated with PEG than in control.  The percentage of empty rice grain reached 84.6% at PEG -0.52 MPa, while at PEG -0.25 MPa the percentage of empty grain was 67.8%.  In control plant, the percentage of empty grain was 10.3%.  Drought stress did not alter the expression of OsPIP2;7.  Keywords: drought, gene expression, IR64, PEG, rice
Potential of Calliandra calothyrsus Leaf Extract to Maintain Estrogen Concentration and Uterine Thickness in Rats Setyawati, Iriani; Wirasiti, Ni Nyoman; Yuni, Luh Putu Eswaryanti Kusuma
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 2 (2021): August 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i2.31063

Abstract

Calliandra calothyrsus Meissn. leaf extract is potential as phytoestrogens. It influenced male mice reproduction, rat estrous cycle, and ovarian histology in previous study. This research aimed to prove the C. calothyrsus leaf extract potential as phytoestrogen source and the effect on endometrial thickness where the embryo implantation take place in early pregnancy. This study used a Completely Randomized Design used 54 days old female rats (Rattus norvegicus). Rats were divided into K as control group (treated with 0.5% Na-CMC as placebo) and P1, P2, and P3 as three groups with C. calothyrsus leaf extract administration with doses of 17.5; 35; and 70 mg/ kg bw respectively. Treatments were given 1 ml/rat/day orally for 20 days. At day 21st, animals were euthanized to collect blood samples for estrogen hormone analysis. After the dissection, all uterus were collected and weighed. Histological preparation was done with paraffin method and Hematoxylin-Eosin staining. The effective dose was 70 mg/ kg bw that did not decrease the weight of the uterus and the body. This dose even maintained the normal diameter and thickness of uterine walls (endometrium, myometrium, and perimetrium layers) like control rats. The extract in this study could increase estrogen concentration in female rats. This research novelty is that C. calothyrsus leaf extract (70 mg/ kg bw) can be used as an alternative herbal suplement to maintain uterine wall thickness and estrogen concentration in productive women. With further clinical research, this extract is a good candidate as potential estrogen source to overcome women infertility or pregnancy difficulties due to problem of endometrial thickness and lack of endogenous estrogen.
PELATIHAN HIDROPONIK DI SMAN 1 DENPASAR, BALI M. Pharmawati; N. N. Wirasiti; I.G.A.S. Wahyuni; R. Kawuri
Buletin Udayana Mengabdi Vol 16 No 2 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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Abstract

Hidroponik merupakan salah satu teknik budidaya tanaman khususnya sayuran yang memiliki beberapa keuntungan antara lain tidak memerlukan lahan yang luas, nutrisi seimbang, bebas gulma serta produksi yang tinggi. Teknik budidaya sayuran secara hidroponik dapat memanfaatkan pekarangan rumah atau halaman sekolah. Oleh karena itu, diperlukan kegiatan pengenalan bertanam sayuran secara hidroponik. Tujuan kegiatan in adalah memperkenalkan dan melatih bertanam sayuran secara hidroponik bagi siswa SMAN 1 Denpasar. Metode yang digunakan terdiri dari metode ceramah, praktek dan diskusi yang meliputi pengenalan jenis-jenis tanaman sayuran, pengenalan berbagai teknik hidroponik serta teknik bertanam mulai dari persemaian, pembibitan, penanaman dan pemeliharaan. Sistem hidroponik yang dipilih adalah sistem hidroponik statis sederhana dengan menggunakan wick (sumbu). Kegiatan pertama di SMAN 1 Denpasar diikuti oleh 18 siswa dan dua guru pendamping. Evaluasi dilakukan melalui antusias dan keaktifan murid serta pertumbuhan tanaman yang dipraktekkan. Sumbu yang terbuat dari kain flannel mengakibatkan pertumbuhan tanaman yang lebih baik dibandingkan sumbu kompor. Kegitan kedua merupakan pengenalan teknik hidroponik aktif diikuti oleh 8 orang siswa dan 2 guru pendamping.
PELESTARIAN FLORA DI LINGKUNGAN PURA TAMAN AYUN MENUNJANG ATRAKSI NOMINASI WARISAN BUDAYA DUNIA Ni Nyoman Wirasiti; I Ketut Ginantra; A.A.G. Raka Dalem; I Ketut Muksin
Bumi Lestari Journal of Environment Vol 11 No 1 (2011)
Publisher : Environmental Research Center (PPLH) of Udayana University

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Abstract

Taman Ayun is a tourist destination which is proposed as a world cultural heritage. Thus, the data about Taman Ayun are important things to be collected to support the proposal. This study is expected to provide data on flora. Data on flora were gathered based on site observation on September 5, 2008. The data collection was also supported by other data collecting techniques such as interview and literature review. Result of the study showed that there were 125 plant species around the temple. As much as 16,8 % were classified as rare/threatened or endangered, 1,6% rare and protected, and the rest (81,6 %) were not protected and not rare species. The highest proportion of the plant (30 %) were decorative plant. Others were material for ceremony (upakara) (21%), food and vegetables (19 %). All of those were covering 70 % of the plant. The rest were for medicine, providing shaded spots, animal fodder, other use and has not been used.
JENIS POHON, PEMANFAATAN SERTA KEPERCAYAAN MASYARAKAT DESA PELIATAN, KECAMATAN UBUD, GIANYAR-BALI I W. Suarna; A. A. G. R. Dalem; N. N. Wirasiti
Bumi Lestari Journal of Environment Vol 6 No 1
Publisher : Environmental Research Center (PPLH) of Udayana University

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Abstract

A study on identification of trees species, their use as well as what the communitybelieve about them held in Peliatan Village, Ubud District, Gianyar Regency (Bali) onFebruary, 2005. The study was carried out by taking a walk along the roads or trails whichwere assessed for their potentials as tracking trails in this village while taking notes on thetree species observed. The trees then were determined their status, their use as well as whatthe community believe about them. Results of this study show that there were 148 kinds oftrees which have been identified on this village. From the use perspective, it was revealedthat these trees could give a wide range of benefits for the community of Peliatan as well asBali in general. Their use for decorations, provides shady spots, as well as uncultivated treeswere the highest in their proportion (22%). In addition, in this village they were alsoidentified 18% of trees which believed to provide a possitive or negative impacts to humans.Most of them (73%) were believed to be related to positive impacts to humans.
JENIS DAN MANFAAT FLORA PULAU DERAWAN KABUPATEN BARAU PROVINSI KALIMANTAN TIMUR Ni Nyoman Darsini; I .G. A. Sugi Wahyuni; A.A.K. Darmadi; Ni Nyoman Wirasiti
Bumi Lestari Journal of Environment Vol 8 No 1 (2008)
Publisher : Environmental Research Center (PPLH) of Udayana University

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Abstract

A study on identication of plants species available on Derawan Island, Berau Regency, East Kalimantan has been conducted based on exploration survey around the island between 2002-2005. The plants whicht have observed were then listed on a table, their scientific names wrre recorded, their status (whether being protected or not) were determined, and the benefits or use of the plants were also listed. From this study it can be found 45 species of 30 families. They may bring benefits for the locals as vegetables, source of medicine, providing shady spots, or decorative plants.
JENIS-JENIS DAN KARAKTERISTIK BURUNG YANG DITEMUKAN DI KAWASAN BEDUGUL DAN SEKITARNYA N. N. Wirasiti; N. M. Rai Suarni; A. A. G. Raka Dalem
Bumi Lestari Journal of Environment Vol 5 No 2
Publisher : Environmental Research Center (PPLH) of Udayana University

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Abstract

A study on birds (species and their characteristics) was carried out in Bedugul(Bali) between November and December 2004. Observation was made along transectlines which are set prior to the study. Species of birds observed were identified andrecorded. In addition, their distributions, status, source of food, reproductive seasons, aswell as migration patterns (whether migrant species or not) were determined. Thisstandard sampling strategy was also supported by casual observation and interview.Results of this study showed that Bedugul and surrounding area have a significantrole in conservation of Bali birds, because at least 56 species of birds have beenidentified in this area, which comprises of 18% of Bali birds. Conservation priorityshould be put on 8 species (14%) of the birds which are protected by law, as well as on 7% of endemic species (for Java and Bali). In addition, conservation effort should be alsogiven priority to 9% of birds that considered to be rare and potentially that be locallyextict. Habitat conservation should becoming focus of attention since it determines theavailability of food sources for those birds. Conservation on forest, plantation as well asshrubs should become focus of attention since these become habitats of more than a half(57%) of birds available in this area.
SUBSTITUTION OF COMMERCIAL FEED WITH MORINGA LEAF MEAL TO IMPROVE THE PERFORMANCE OF MALE RABBIT N.M.R. Suarni; N.G.A.M. Ermayanti; N.N. Wirasiti
JURNAL WIDYA BIOLOGI Vol 12 No 01 (2021): Widya Biologi
Publisher : UNHI Press

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Abstract

The aims of this study were to find out the effects of moringa leaf meal substitution in commercial feed on the performance of male rabbits (bucks). Twenty four four months old male rabbits with an average live weight of 1200g were used in this study. There were four treatments in this study including 0% (K0) as control and 15% (K1), 30% (K2), 45% (K3) substitution of moringa leaf meal in commercial feed and each treatment consisted of six replicates. The treatments were carried out for two months. The variables measured were including body weight, feed consumption, feed conversion. The results showed that there were significant differences (P <0.05) performance between all treatments (K1, K2, K3) and the control (K0). Substitution of moringa leaf meal up to 45% in commercial feed increase performance of male rabbit. It was concluded that the moringa leaf meal might be substituted in commercial feed up to 45 % to improve the performance of male rabbits (bucks). To get maximum performance of male rabbit rabbits it should be better substituted the commercial feed with moringa leaf meal is 35%.
FORMULASI EKSTRAK ETANOL DAUN MALAKA (Phylanthus emblica) DALAM SEDIAAN MASKER GEL PEEL-OFF Agustina Bayo Inggirik Mogan; Ni Luh Arpiwi; Ni Nyoman Wirasiti
SIMBIOSIS Simbiosis Volume 12 No. 1 2024
Publisher : Program Studi Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2024.v12.i01.p01

Abstract

The use of facial masks is useful for softening the skin, nourishing and opening clogged pores, helping prevent premature aging, and reducing the appearance of fine lines and wrinkles. Plants that can be used as active ingredients are malaka, or amla (Phyllanthus emblica L.), a natural source of antioxidants. This study aims to determine the content of phytochemical compounds in the ethanol extract of Malaka leaves, to analyze the antioxidant activity and IC50 of the ethanol extract of Malaka leaves, to determine the quality of the peel-off gel mask with the addition of the concentration of the ethanol extract of Malaka leaves and to find out which formulation is preferred by Probandus. The research design used a completely randomized design (CRD) using Malaka leaf extract with concentrations F0(0%), F1(1%), F2(2%) and F3(3%). The phytochemical content test of Malaka leaf extract showed the presence of flavonoids (0.467%) and tannins (10.97%). The antioxidant activity of the ethanol extract of Malaka leaves was 1.3314% and IC50 was 30 ppm. The organoleptic test results for all preparations did not change during 5 weeks of storage, pH 5.37-5.54, the viscosity of the mask preparation was 50000-126000 cps, the peel-off gel mask preparation was homogeneous and did not . Statistical analysis of the
HUBUNGAN ANTARA RIWAYAT KETURUNAN DAN FAKTOR RISIKO TERHADAP KEJADIAN ASMA: THE RELATIONSHIP BETWEEN HEREDITARY HISTORY AND RISK FACTORS FOR ASTHMA Prita Jaya, Made Ayu Mutiara; Junitha, I Ketut; Wirasiti, Ni Nyoman
SIMBIOSIS Simbiosis Volume 13 No. 2 2025
Publisher : Program Studi Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2025.v13.i02.p03

Abstract

ABSTRACT Asthma is a disease caused by chronic inflammation of the respiratory tract, which causes shortness of breath and difficulty breathing. This disease arises due to genetic factors or heredity. Genetic diseases may not be inherited again in the next generation, but can recur due to several risk factors that trigger asthma. This study aims to analyze the relationship between hereditary history and risk factors on the incidence of asthma. Sampling was carried out using a purposive sampling technique, data collection was carried out by measuring the variables studied using questionnaires and interviews, and data analysis was carried out using correspondence analysis. The results of the study show that not all asthma patients have a family history of asthma, and non-genetic risk factors that cause asthma recurrence consist of age, gender, body mass index (BMI), home location, smoking habits, and environmental factors consisting of fatigue, cold weather, dust allergies, mental influences, air pollution, animal dander allergies, and food allergies. Keywords: Allergies, asthma, genetics, gender, shortness of breath, age
Co-Authors A. A. G. R. Dalem A.A.S. A. Sukmaningsih AGUNG EKO CAHYA KUSUMA PUTRA Agustina Bayo Inggirik Mogan Anak Agung Gde Raka Dalem Anak Agung Ketut Darmadi Andi Ratu Bulqis I GUSTI AYU MANIK ERMAYANTI I Gusti Ayu Manik Widhyastini I Gusti Ayu Sugi Wahyuni I Ketut Ginantra I Ketut Junitha I KETUT JUNITHA I Ketut Junitha I KETUT MUKSIN I W. Suarna I.G.A.S. Wahyuni Ida Bagus Bajing Agastya IRIANI SETYAWATI Kezia Artanauli Purba LUH Puru ESWARYANTI KUSUMA YUNI Luh Putu Wrasiati MADE PHARMAWATI N.G.A.M. Ermayanti Ni Luh Arpiwi Ni Made Rai Suarni NI NYOMAN DARSINI Ni Putu Putri Wulandari Ni Putu Sukma Widyantari Ni Wayan Sudatri Prita Jaya, Made Ayu Mutiara Retno Kawuri Sally Nabila Rizal Septiasari, Ni Putu Senshi Tyara Tyara Yoseph Raymond Irawan