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Green and Tropical Laboratory for Sustainability
Published by Universitas Muhammadiyah Malang
ISSN : -     EISSN : 30645069     DOI : https://doi.org/10.22219/gtlabs.vxiy.xxxxx
Core Subject : Science, Agriculture, Social, Engineering,
Agriculture, Biological Sciences & Forestry Chemical Engineering, Chemistry & Bioengineering Decision Sciences, Operations Research & Management Environmental Science Social Sciences
Green And Tropical Laboratory For Sustainability encompasses a broad range of research areas related to the Sustainable Development Goals (SDGs), including but not limited to: Laboratory-Based Studies on Tropical Ecosystems: Research on biodiversity, climate change, ecosystem restoration, and natural resource management within tropical regions. Development and Application of Laboratory Technologies for Sustainable Energy: Investigations into renewable energy sources, energy efficiency, and clean energy technologies that contribute to sustainable development. Laboratory Research on Waste Management and Pollution Control: Studies focused on waste treatment, recycling, circular economy practices, and pollution mitigation strategies. Laboratory Investigations into Food Security and Sustainable Agriculture: Research on organic farming, agroforestry, food safety, nutritional value, and sustainable agricultural practices. Laboratory Studies on Human Health and Environmental Interactions: Exploration of environmental health risks, infectious diseases, toxicology, and public health interventions. Research on Digital Governance for Sustainable Development: Studies examining the role of digital governance in enhancing sustainability efforts, including the application of digital tools and technologies in managing environmental resources, monitoring ecological impacts, and supporting policy-making processes. Social and Educational Research in the Context of Sustainability: Investigations into social dynamics, educational initiatives, and community engagement strategies that align with sustainability goals, particularly in tropical regions. This includes the integration of sustainability concepts into educational curricula and the promotion of social practices that support green and sustainable development.
Arjuna Subject : Umum - Umum
Articles 15 Documents
 1   2 
Tropical laboratory research to support sustainability issues: A systematic literature review H. Husamah; Abdulkadir Rahardjanto; Ahmad Adnan Mohd Shukri
Green and Tropical Laboratory for Sustainability Vol. 1 No. 1 (2024): August
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/gtlabs.v1i1.36286

Abstract

Background: The study of tropical laboratory research to support sustainability issues is very urgent to ensure that efforts to conserve and utilize natural resources in tropical areas can be carried out effectively and sustainably. Objectives: This SLR focuses on analyzing research trends in relation to “tropical laboratory research to support sustainability issues”. Methods: This SLR will provide a relatively complete analysis so that it can inspire research activities for researchers, especially in countries that do not yet have a high interest in this aspect. This investigation constitutes a SLR to systematically identify, assess, and analyze all specific research inquiries, topics, or areas. The sequence of inclusion and exclusion was using PRISMA. Data simulation uses "Analyze search results" which is available on the Scopus system. To enrich data and analysis, the data was exported to *CSV format (for visualizing data process with VOSviewer and RStudio) and *RIS (for synchronized with Mendeley). The final result of this process was 17 articles that met the criteria and were worthy of analysis. Results: We discuss the findings related to Tropical Laboratory Research to Support Sustainability Issues based on the established research questions. Conclusion: Publications related to tropical laboratory research for sustainability have shown significant fluctuations since 2004, with the majority of publications coming from the United States. While there is potential for future growth, it is important to increase funding support and collaboration between countries to make this research more effective in supporting sustainability.
Immunostimulanting effect of jackbean flour on non-specific immunity of mice in vitro and in silico Riris Dewi Retnowati; Moh. Mirza Nuryady; Elly Purwanti; Sri Wahyuni; Iin Hindun
Green and Tropical Laboratory for Sustainability Vol. 1 No. 1 (2024): August
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/gtlabs.v1i1.36289

Abstract

Background: Jackbean (Canavalia ensiformis) contains protein and secondary metabolite compounds, such as phenolics and flavonoids that can have potential as antioxidants, antibacterial, antimicrobial, and antiviral. Objectives: This study aims to determine the effect of giving jackbean flour as an immunostimulant on non-specific immunity of mice in-vitro and in-silico. Methods: This type of research is double methods research with a comparative method and uses an experimental design in the form of a Randomized Group Design and in-silico testing with the NCBI website. Mice with a total of 16 heads were divided into 4 treatment groups, namely the Negative Control group (P0), Dose 1 (P1) with a solution of jackbean flour as much as 2.3 g, Dose 2 (P2) as much as 4.6 g, Dose 3 (P3) as much as 9.2 g. The hemagglutination test was carried out on mice with a total of 16 mice. The hemagglutination test was carried out on the 15th day after giving the flour solution, then tested the content of active compounds in koro pedang in silico according to the literature review of previous research from the website which contains active compounds, namely canavanine, concanavalin A and B. Results: The results of in-vitro research show that the most influential dose for hemagglutination is P1 with a dose of 2.3 grams, characterized by almost all wells on the microplate there is concentrated clotting, and there is significance (p < 0.05) which means there is a difference in the effectiveness of jackbean flour solution as an immunostimulant from each dose. Conclusion: This study concluded that jack bean flour, especially at a dose of 2.3 grams, effectively enhanced non-specific immunity in mice, as evidenced by significant hemagglutination results. Therefore, jack bean flour has the potential to be a natural immunostimulant in improving immune health and fighting infections.
Identification histological structure of femur and antebrachium Oryctolagus cuniculus as a biology learning Aliffian Nisa’ Cholida; Sri Wahyuni
Green and Tropical Laboratory for Sustainability Vol. 1 No. 1 (2024): August
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/gtlabs.v1i1.36301

Abstract

Background: Preparations are used as learning resources in histology practicum, for this reason, it is necessary to seek various ways to improve the quality, one of which is the manufacture of femoral and antebrachium preparations of Oryctolagus cuniculus. The rubbing preparations were obtained through the microtechnical method by boiling and rubbing the bones as thinly as possible. Objectives: The purpose of this study was to identify the histological structure of the femur and antebrachium tissue of Oryctolagus cuniculus which could be observed microscopically through bone rub preparations. Methods: This research method is descriptive. The research sample is taken from the femur and antebrachium Oryctolagus cuniculus. The data collection method was by direct observation of the preparations using a microscope and documented using an HP Realme camera directly from the microscope. The data analysis technique was carried out in a qualitative descriptive manner. The research was conducted at the Biology Laboratory of the University of Muhammadiyah Malang Results: Unstained femur and antebrachium preparations of Oryctolagus cuniculus show parts of the haversian system, namely Canalis havers, Osteocytes, Lacunae, Canaliculi, Lamella, and Canalis Volkmann. Conclusion: The research results can be used as learning resources or histology practicum media.
Neem leaves (Azadirachta Indica) and Jasmine flowers (Jasminum sambac): A toxic combination of Aedes aegypti Putri Ayu Irodah; Moh. Mirza Nuryady; N. Nurwidodo
Green and Tropical Laboratory for Sustainability Vol. 1 No. 1 (2024): August
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/gtlabs.v1i1.36335

Abstract

Background: The high number of cases of dengue hemorrhagic fever (DHF) in Indonesia indicates the lack of diseases prevention caused by mosquito vectors. Chemical control efforts can cause resistance to mosquitoes, so it is necessary to have natural insecticides derived from plants, one of which is neem and jasmine which has active ingredients as larvicides. Objectives: This research aims to analyze the effect of different concentrations of extracts from neem (Azadirachta indica) and jasmine (Jasminum sambac) leaves on the mortality of Aedes aegypti larvae. Methods: This research is true experimental research using 7 treatments, there are negative control group using distilled water and a positive control group using 1% abate and 5 treatment groups using combination of extracts from neem leaves (Azadirachta indica) and jasmine flower (Jasminum sambac) with concentration ratio 0%:100%, 25%:75%, 50%:50%, 75%:25%, dan 100%:0%. In this study, it is used Aedes aegypti larvae taken by landing collection in Nguling, Pasuruan. Results: The results of identification of larvae showed that the larvae taken were Aedes aegypti larvae in terms of morphological characteristics. The results showed that the combination of 50%: 50% concentration caused a lot of mortality in larvae and obtained LC50 from the extract of neem (Azadirachta indica) and jasmine flower (Jasminum sambac) was 253,57 µg / mL. Conclusion: Extracts from neem leaves (Azadirachta indica) and jasmine flowers (Jasminum sambac) can be used as an candidate alternative to Aedes aegypti larvicide.
Aedes aegypti mosquitoes in Malang Regency: What is the resistance status to malathion 0.8%? Jihan Ully Agustin; Siti Zaenab; Moh. Mirza Nuryady; Tutut Indria Permana
Green and Tropical Laboratory for Sustainability Vol. 1 No. 1 (2024): August
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/gtlabs.v1i1.36354

Abstract

Background: Dengue hemorrhagic fever (DHF) carried by the Aedes aegypti has become a health problem in Karangploso, Singosari, and Lawang Districts. The vector control program implemented by the government is fogging. Fogging contains an active material malathion which can hit adult mosquitoes. Excessive use of malathionic active ingredients can lead to vector resistance. Objectives: The purpose of this study is to analyze the resistance status of Ae. aegypti to malathion 0.8% in Karangploso, Singosari, and Lawang Districts, as well as community behavior in controlling dengue disease. Methods: Type of research is a descriptive observational study with cross sectional approach. Data was obtained through susceptibility tests in the laboratory and observations of public attitudes in controlling dengue disease through a Google form survey. The data was presented in tabular form and discussed descriptively. The results showed that the mortality percentage of Ae. aegypti mosquitoes in Karangploso District was 98%, Singosari 96%, and Lawang 98%, all of which were in the tolerant category. Results: The results of observations to the community showed that the community already knew the most common breeding sites for Ae. aegypti larvae is bathub and the way people control dengue fever using several types of household insecticides, namely spray, burn, and anti-mosquito lotions. Conclusion: The conclusion of this study is the resistance status of Ae. aegypti in the three sub-districts are classified as tolerant and the community already knows the most preferred breeding sites for the Ae. aegypti, but the way people control DHF is still using some household insecticides that may increase the status of Ae. aegypti becomes resistant.
In silico test of cytotoxic activity and toxicity of guava leaf compounds (Psidium guajava) as a drug for diabetes mellitus Aisha Azaria Nisa’ul Khoiro; Moh. Mirza Nuryady
Green and Tropical Laboratory for Sustainability Vol. 2 No. 1 (2025): August
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/gtlabs.v2i1.38845

Abstract

Background: Diabetes mellitus is a metabolic disorder caused by a lack of the hormone insulin. Diabetes mellitus is characterized by elevated blood glucose levels due to the inactivity of the GLP-1 protein, which leads to decreased insulin function. Guava leaves (Psidium guajava) are suspected to have antidiabetic effects, but the active compound responsible for lowering blood glucose levels is unknown. Objectives: The aim of this study was to determine the physicochemical properties and toxicity as well as the mechanism of active compounds of guava leaves using In Silico studies. Methods: The method in this research is a computer-based experiment in the form of Molecular docking, by comparing the active compounds in guava leaves with the target protein GLP-1. Results: The results of the In Silico test show that the Caryophyllene and Cadala compounds have binding affinities of -7.8 and -6.9 and the toxicity class for caryophyllene is class 6, which indicates that the compound is not toxic, while for cadala the toxicity class is included in class 5, which indicates that it may be harmful if swallowed. Conclusion: The compounds Cadala and Caryophyllene demonstrated that further testing is warranted to determine the proportions required to maintain their safety. Meanwhile, benzene was not considered a drug candidate because its carcinogenicity was shown to be active in toxicity tests, suggesting it cannot be used as a drug candidate for diabetes mellitus.
Information technology-based waste management at the Sukoharjo Pati landfill with an importance performance analysis approach Metadelphi Werdianto; Nany Yuliastuti; Mochamad Arief Budihardjo
Green and Tropical Laboratory for Sustainability Vol. 2 No. 1 (2025): August
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/gtlabs.v2i1.38857

Abstract

Background: Waste management is a crucial issue in achieving a clean and sustainable environment, necessitating the use of environmental information technology to improve its effectiveness. However, the effectiveness of this technology in waste management at final disposal sites still requires further analysis to meet public expectations and satisfaction. Objectives: This study aims to analyze environmental information technology-based waste management at the Sukoharjo Landfill, Pati Regency, using the Importance Performance Analysis (IPA) approach. Methods: The evaluation method is used to measure the effectiveness of waste management with data collected through surveys, interviews, observations, and literature studies. Data analysis was carried out quantitatively using the IPA method and qualitatively using descriptive-interactive techniques based on the stages of Miles and Huberman. Results: The results of the study show: (1) waste management includes all stages of management, namely planning, organizing, implementing, monitoring, evaluating, and following up; (2) environmental information technology is effective and satisfies the community, with 86.27% of respondents rating its performance as very good and 92.85% feeling satisfied; and (3) the IPA approach shows very high management performance (80.03%) and community expectations are met (87.13%). Conclusion: The results of the study show that the use of environmental information technology in waste management at the Sukoharjo Landfill has proven effective and is able to meet expectations and increase community satisfaction.
In silico test of cytotoxic activity and toxicity of bioactive compounds of Aloe vera extract as a candidate drug for diabetes mellitus Eva Ayuntya; Moh. Mirza Nuryady
Green and Tropical Laboratory for Sustainability Vol. 2 No. 1 (2025): August
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/gtlabs.v2i1.38873

Abstract

Background: Diabetes mellitus is a disease characterized by hyperglycemia and metabolic disorders associated with absolute or relative deficiency in insulin action and/or secretion. Aloe vera contains several compounds with antihyperglycemic effects, and Aloe vera can mimic insulin and lower blood glucose levels. Objectives: The aim of this research is to determine the predicted physicochemical properties, toxicity and mechanisms of active compounds in Aloe vera. Methods: The method used is a computer-based experimental Molecular Docking, by predicting physicochemical, toxicity tests, bioactivity tests, protein and ligand preparation, score analysis, validation of molecular docking results, and 3D molecular visualization analysis by comparing three active Aloe vera compounds with the target protein GLP-1. Results: In silico results showed that sinapic acid, catechin, and quercitrin have good physicochemical, pharmacokinetic, and toxicity properties. In silico testing of the active Aloe vera compounds showed binding values of less than 10 with the same amino acid residues in the catechin and quercitrin compounds that bind to the active site of the GLP-1 protein. Conclusion: The active compounds contained in Aloe vera, sinapic acid, catechin, and quercitrin, can be predicted to be used as candidates for diabetes medication.
In silico study: Candidate compounds of Jatropha curcas L. plant as breast cancer inhibitors Desti Amalia Ramadhayanti; Moh Mirza Nuryady
Green and Tropical Laboratory for Sustainability Vol. 2 No. 1 (2025): August
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/gtlabs.v2i1.38880

Abstract

Background: Cancer is a disease caused by the growth of abnormal cells in the body, which can grow and attack any part of the body. When someone has cancer, the microRNAs in the body play a role in preventing cancer from multiplying. Therefore, inhibiting microRNAs could be a solution for developing cancer drugs. One plant that can be used as a natural chemotherapy agent is Jatropha curcas L., also known as the castor oil plant. Objectives: Jatropha curcas plants can be used as an alternative to petroleum, namely in the manufacture of biodiesel and as a raw material for treating diseases including cancer, skin diseases, respiratory diseases, and infectious diseases. Methods: The research method is the 3D structure of Sirtulin1 protein (pdb id: 4I5I), β-sitosterol and 2-(Hydroxymethyl)-2 nitro-1,3-propanediol prepared with the PyRx program. Molecular docking was analyzed by the interaction of β-sitosterol and 2-(Hydroxymethyl)-2 nitro-1,3-propanediol with Sirtulin1 protein using Autodock 4.2 software. and visualized with discovery studio version 4.1. Data analysis used descriptively. Results: The results of the study of β-sitosterol and 2-(Hydroxymethyl)-2 nitro-1,3-propanediol with sirtulin1 protein produced 12 amino acid residues in the form of SER275, ASN465, SER442, ASP272, GLU467, PHE321, CYS380, ARG384, ILE387, PHE321, PHE366, PHE388. The presence of this bond indicates that the test ligand can bind to the amino acids present in the Sirtulin1 protein. Conclusion: Based on the docking results, it is known that the bonds that occur in the 2-(Hydroxymethyl)-2 nitro-1,3-propanediol ligand and the Sirtulin1 protein are Conventional Hydrogen Bond and Carbon Hydrogen Bond bonds. Hydrogen bonds are the strongest bonds among covalent bonds so they are more widely distributed and produce pharmacological activity. Thus, compounds that have amino acid residues in the form of hydrogen bonds are stronger and produce high inhibitory activity. The presence of these bonds indicates that the test ligand can bind to the amino acids in the Sirtulin1 protein.
Effect of the 10% nitric acid and 10% chromic acid ratio on the quality of maceration preparations Ariella Junes Egalita; Farida Indirahma; Auberta Myra Ardelia; Dhiga Agung Sasongkojati; Aliffian Nisa Cholida; Sri Wahyuni
Green and Tropical Laboratory for Sustainability Vol. 2 No. 2 (2025): December
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/gtlabs.v2i2.39208

Abstract

Background: Maceration is a microtechnique used to examine xylem vessel anatomy by softening plant tissues in specific solutions so that tracheary elements can be observed clearly. However, the optimal ratio of acid solutions for producing durable and well-stained maceration slides is still rarely reported for Piper betle. Objectives: This study compared two ratios (1:1 and 1:5) of 10% nitric acid and 10% chromic acid to evaluate their effects on the quality of maceration preparations from green betel (Piper betle) stems. Methods: An experimental design was applied. Stem and petiole samples were processed through boiling, acid soaking, staining with safranin and methylene blue, graded alcohol dehydration, and fixation/clearing using xylol. Observations were conducted under 400× magnification, and findings were summarized descriptively Results: The 1:1 ratio produced more uniform staining and more resilient preparations than the 1:5 ratio. Slides prepared with the 1:5 ratio tended to be more fragile and exhibited less optimal staining, likely due to excessive chromic acid. Methylene blue staining was weak in both treatments, consistent with the dye’s high water solubility and possible incomplete rinsing after acid treatment. Conclusion: Maceration was effective for observing xylem anatomical structures in P. betle. A 1:1 ratio of 10% nitric acid to 10% chromic acid is recommended to obtain better slide quality and durability for microscopic observation.

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