cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
-
Contact Email
-
Phone
-
Journal Mail Official
-
Editorial Address
-
Location
Kota adm. jakarta pusat,
Dki jakarta
INDONESIA
JURNAL SAINS DAN TEKNOLOGI INDONESIA
Published by Badan Pengkajian dan Penerapan Teknologi
ISSN : -     EISSN : -     DOI : -
Core Subject : Science, Education,
Computer Science & IT Education
Arjuna Subject : -
Articles 12 Documents
 1   2 
Search results for , issue "Vol. 14 No. 1 (2012)" : 12 Documents clear
TRANSFORMASI PLASMID PTRLI DENGAN TEKNIK ELEKTROPORASI PADA ASPERGILLUS TERREUS DAN UJI STABILITAS TRANSFORMAN hardianto, dudi; gusnidar, tutus; singgih, marlia; musadad, amir; sumaryono, wahono
Jurnal Sains dan Teknologi Indonesia Vol. 14 No. 1 (2012)
Publisher : Badan Pengkajian dan Penerapan Teknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (664.551 KB) | DOI: 10.29122/jsti.v14i1.901

Abstract

Aspergillus terreus is a Saprophyte fungus that produces several secondary metabolites as lovastatin (anti-cholesterol drug) and itaconic acid (a polymer material). Lovastatin is one of the statin class of drugs that have efficacy as antihypercholesterolemic. Plasmid transformation is the introduction and incorporation of exogenous plasmid into cells orprotoplast. In this study, pTRLI plasmid (pTRI inserts containing lovE gene as a regulator gene in the biosynthesis of lovastatin) will be transformed by electroporation transformation. The purpose of this research is transformation of pTRLI plasmid into protoplasts of Aspergillus terreus by electroporation and obtain stable transformants. The research was initiated by isolation of pTRLI plasmid. Then pTRLI plasmid was determined purity and concentration by nanodrop. Furthermore, Protoplasts of Aspergillus terreus were isolated enzymatically by adding an enzyme which can degrade the cell wall of Aspergillus terreus which contains chitin and cellulose. PTRLI plasmid were transformed into protoplasts of Aspergillus terreus by electroporation. These transformants were grown in Czapek-Dox medium containing pyrithiamine agar and the number of transformants mg-1 of pTRLI plasmid was calculated. Transformants were selected to grow in Czapek-Dox medium containing piritiamin 1 mg l-1. The number of transformants produced 187 transformants mg-1 of PTRLI plasmid. Transformants are stable up to five generations by growing the transformants in Czapek-Dox medium agar containing piritiamin 1 mg l-1. The success of the transformation indicated by ptrA gene in transformants that can be amplified by PCR. The size of fragment DNA is 801 bp.
KELAYAKAN TEKNO-EKONOMI MIGRASI TEKNOLOGI PROSES PRODUKSI GULA KRISTAL PUTIH DARI SULFITASI KE DEFEKASI REMELT KARBONATASI Subiyanto, Subiyanto
Jurnal Sains dan Teknologi Indonesia Vol. 14 No. 1 (2012)
Publisher : Badan Pengkajian dan Penerapan Teknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (96.617 KB) | DOI: 10.29122/jsti.v14i1.906

Abstract

Sugar is a strategic commodity for Indonesia, such that the availibility and the price have been managed by the government. The national production of Indonesia sugar has not been enough to cover the consumption, such that the deficit must be imported. Indonesian sugar was produced by 61 factories, where 51 units are State-owned Company. Unfortunately, the quality of sugar produced by State – owned factory has been getting worse, such that some are not qualified to the Indonesian National Standards (SNI). One of the reasons is poor process technology adopted, which is mostly using sulfitation technology. The Government, therefore, encourages industry to migrate the process technology from sulfitationsystem to defecation remelt carbonatation (DRK) system. This study assesses the techno-economy feasibility of the migration. The results indicate that technology migration is technically feasible, but for the business, Government needs to give price incentive to the product. Furthermore, the study recommends that adoption of DRK technology should be prioritized to the factories with abundant stock of baggase.

Page 2 of 2 | Total Record : 12

 1   2 

Filter by Year

2012 2012


Reset
Filter By Issues
All Issue Vol. 17 No. 3 (2015) Vol. 17 No. 2 (2015) Vol. 17 No. 1 (2015) Vol. 16 No. 3 (2014) Vol. 16 No. 2 (2014) Vol. 16 No. 1 (2014) Vol. 15 No. 3 (2013) Vol. 15 No. 2 (2013) Vol. 15 No. 1 (2013) Vol. 14 No. 3 (2012) Vol. 14 No. 2 (2012) Vol. 14 No. 1 (2012) Vol. 13 No. 3 (2011) Vol. 13 No. 2 (2011) Vol. 13 No. 1 (2011) Vol. 12 No. 3 (2010) Vol. 12 No. 1 (2010) Vol. 11 No. 3 (2009) Vol. 11 No. 2 (2009) Vol. 11 No. 1 (2009) Vol. 10 No. 3 (2008) Vol. 10 No. 2 (2008) Vol. 10 No. 1 (2008) Vol. 9 No. 3 (2007) Vol. 9 No. 2 (2007) Vol. 9 No. 1 (2007) Vol. 8 No. 3 (2006) Vol. 8 No. 2 (2006) Vol. 8 No. 1 (2006) More Issue