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INDONESIA
JURNAL SAINS DAN TEKNOLOGI INDONESIA
Published by Badan Pengkajian dan Penerapan Teknologi
ISSN : -     EISSN : -     DOI : -
Core Subject : Science, Education,
Computer Science & IT Education
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Articles 12 Documents
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Search results for , issue "Vol. 14 No. 3 (2012)" : 12 Documents clear
MEKANISME PELEPASAN HORMON GONADOTROPIN (GtH-II) IKAN LELE (Clarias sp) SETELAH DI INDUKSI LASERPUNKTUR PADA TITIK REPRODUKSI P.S.W, kusuma; Marhendra, Agung P.W.; Aulanni’am, Aulanni’am
Jurnal Sains dan Teknologi Indonesia Vol. 14 No. 3 (2012)
Publisher : Badan Pengkajian dan Penerapan Teknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (99.672 KB) | DOI: 10.29122/jsti.v14i3.928

Abstract

Induction laserpuncture effectively stimulates gonad maturation and spawning, but the associated gonadotropin releasing hormone (LH / GtH-II) mechanism is still unknown. So the purpose of this study demonstrate the mechanism of the release of GTH-II on catfish (Clarias sp.) after induction laserpuncture at the reproduction acupoint. The test fish consisted of 54 males and 54 females aged 8-9 months from the F1 hybrid Sangkuriang type female and Paiton type male parents. Ourstudy employed an experimental method with completely randomized design. The treatment comprised 6 levels with 9 repetitions. We were to observe gonadotropin hormone (GtH-II) concentrations pre-spawn, spawn, post-spawning in the laserpuncture exposed. As a comparison investigations were also conducted to untreated (control). Blood sampling performed at 6 h after induction laserpuncture. Hormone levels using the Elisa test kits Fish Luteinizing Hormone (LH) BIOTECH CO. CUSABIO., LTD. by Catalog No.. CSB-E15791Fh. The test results significant effect (P<0.05) increased levels of GtH-II profiles between laserpuncture inductionwith control, which profiles GTH-II levels pre-spawn conditions higher 2.2468, as well as the higher spawn condition 1,5409 in comparison with controls. However, the condition post-spawning in induction laserpuncture lower value 1.1131 compared with controls. This suggests that induction laserpuncture at the reproduction acupoint can stimulate of GABA release from GABAergic neurons pre-spawn and spawn condition. GABA stimulates the hypothalamus to release GnRH neurons. GnRH neurons stimulates the pituitary to GtH-II release. GtH-II will affect the acceleration of gonad maturation and spawning.
UJI INOKULASI Fusarium sp UNTUK PRODUKSI GAHARU PADA BUDIDAYA A. Beccariana iskandar, dudi; suhendra, ahmad
Jurnal Sains dan Teknologi Indonesia Vol. 14 No. 3 (2012)
Publisher : Badan Pengkajian dan Penerapan Teknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (762.973 KB) | DOI: 10.29122/jsti.v14i3.924

Abstract

Gaharu (Agarwood) is one of the most valuable of non-timber forest products (NTFPs) commodities in Indonesia that has an important role in economic and income for the community surrounding forest. However, the intensive exploitation and uncontrolled harvest capacity has brought those two potential species tends to decrease, and therefore gaharu is listed in the list of the CITES’ Appendix II. One of the solutions to deal with those cases researchers have been developing gaharu plantation as well technique to produce gaharu products through inoculation. Theaim of this study was to find the effectiveness of four different Fusarium sp from different locations in Indonesia (Parung/F1, Banjarmasin/F2, Jambi/F3 and Gorontalo/F4) in order to produce gaharu. These Fusarium were inoculated to 10 year’s old of A. Beccariana at Penajam, East Kalimantan. The result show that inoculant from Gorontalo (F4) gave the best result and the highest potential to the production of gaharu compare to other Fusarium sp.

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