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Jurnal Akademika Biologi
Published by Universitas Diponegoro
ISSN : -     EISSN : 26219824     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Jurnal Akademika Biologi diterbitkan oleh Departemen Biologi Fakultas Sains dan Matematika Universitas Diponegoro Semarang. Jurnal ini sebagai media publikasi hasil karya ilmiah lulusan S1 Departemen Biologi. Jurnal Akademika Biologi menerima artikel-artikel yang berhubungan dengan bidang ilmu biologi.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 8 Documents
 1 
Search results for , issue "Vol. 2 No. 2 April 2013" : 8 Documents clear
PELACAKAN FRAGMEN GEN PENYANDI ENZIM ß-KETOASIL-ACP SINTASE II (KAS II) DARI MESOKARP KELAPA SAWIT (ELAEIS GUINEENSIS JACQ. L.) Yohanes Chandrawijaya; Teuku Tajuddin; Hermin Pancasakti Kusumaningrum; Anto Budiharjo
Jurnal Akademika Biologi Vol. 2 No. 2 April 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

        The standard of quality is one of the determining values of crude palm oil as an international trade commodity. Better standard of quality for crude palm oil is a constant demand of the market. Quality improvement can be made by increasing the contents of oleic acid in the mesocarp of  E. guinensis. Among the uses of oleic acid are as follows: anti-carcinogenic agent, anti-oxidant, source of pro-vitamin A, and source of Vitamin E. Oleic acid is a form of non-saturated fatty acid encoded by KAS II genes. The expression profiling of KAS II is achieved through isolation of total RNA by Trizol reagent, RNA purification, using DNAse RNAse free, synthesis of cDNA using Reverse Transcriptation PCR approach, and amplification of KAS II genes with Nested PCR approach. The amplification process of KAS II genes is carried out using both internal and external primers. The first step of the external primer PCR is F-KAS-1 and R-KAS-1. Internal primer of PCR in the second step is F-KAS-2 and R-KAS-2. The results of this research are fragments of KAS II genes between 1500–2000 bp. These amplicons are suitable with primers designed at the approximation of 1796 bp. Selection of three amplicons at the annealing temperatures of 54oC, 55.9oC, and 58oC shows good DNA band visualizations. Annealing at 58oC shows the best result with high intensity DNA band and no smear. Further research is needed to determine the accuracy of the amplicons through sequencing step.  Keywords: KAS II, Elaeis guineensis, annealing, Nested PCR, RT-PCR
ISOLASI DAN KARAKTERISASI MORFOLOGI KOLONI BAKTERI ASOSIASI ALGA MERAH (RHODOPHYTA) DARI PERAIRAN KUTUH BALI Aninditia Sabdaningsih; Anto Budiharjo; Endang Kusdiyantini
Jurnal Akademika Biologi Vol. 2 No. 2 April 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Kutuh Beach in Bali is an area established as the Minapolitan algae with a high diversity. Microorganisms associated with marine organisms, usually have secondary metabolites that can be used as a source of drugs, antibiotics, enzymes, and cosmetics. The aimed of this research was to isolate and characterize colony morphology of bacteria associated with red algae based on colony morphology. Seven bacteria were isolated from three samples of red algae that were Kappapycus alvarezii, Gelidiella acerosa and Eucheuma spinosum. The isolates had pigmentation of beige, white and orange. Based on Gram's staining, seven isolates were Gram positive with bacilli and cocci in shape. Keywords : associated bacteria, morphological colony, pigmentation, red algae
UJI KEMAMPUAN PRODUKSI SITOKININ OLEH RHIZOBAKTERI Tri Wijiastuti; Agung Suprihadi; Baedah Rupaedah
Jurnal Akademika Biologi Vol. 2 No. 2 April 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Plant growth regulators have a role in the process of growth and development of a plant. One of the Plant growth regulators which play an important role in process of growth and development is cytokinin. Cytokinin are able to stimulate division and cell enlargement, accelerate formation of organs, delay aging of various types of plants, and increase number and size of leaves. The utilization of rhizobacteria which is capable in producing cytokinin as a basic component of biological fertilizer has a very important role in improving the growth of plants. This research aims to select the capability of rhizobacteria isolates which are able to produce cytokinin and determine morphological characteristics and biochemical characteristics of potential rhizobacteria isolates as a cytokinin producer. The research was conducted at the Laboratory Agromikrobiologi, Biotechnology Research Center, Agency for the Assessment and Application of Technology, Puspitek, Serpong. The research procedure was rhizobacteria cultivation and extraction of cytokinin from rhizobacteria culture, then detect cytokinin using the method of thin layer chromatography (TLC) and high performance liquid chromatography (HPLC). The results showed that the isolates which were capable to producing cytokinin was Jember 2.2 with a concentration of 92 mg L-1 and expected as Pseudomonas genus bacteria. Keywords : biofertilizers, rhizobacteria, cytokinin.
KEANEKERAGAMAN NGENGAT DI WANA WISATA GONOHARJO, LIMBANGAN, KENDAL, JAWA TENGAH Nanang Kamaludin; Mochamad Hadi; Rully Rahadian
Jurnal Akademika Biologi Vol. 2 No. 2 April 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Moths is insect in Order Lepidoptera of Nocturnal habit. Moths help to polinate the flower were blossom in the night. The relationship of moths and plants can be used as bio-indicators of environmental changes in the Gonoharjo forest habitats. The objective of this study are to compare the abundance and diversity of members of moth (Lepidoptera) in various habitats and to determine the status of rare and endemic species that are found in Wana Wisata Gonoharjo. This study used light traps method for moth in four different habitats, i.e., secondary forests, riparian area and forests of pine forest and coffee plantation. The research was conducted over two months during October-November 2012. The analysis used were relative abundance, diversity index, Huctheson test, evennes index and similarity index. Threety nine moth species from 10 families were found in 4 habitats of Wana Wisata Gonoharjo. The highest number of moth species found in secondary forest (30 species) and the lowest one found in the pine forests (6 species). Statistically, there were differences within the types of habitat diversity. Evennes indices in all habitats of Wana Wisata Gonoharjo were relatively high. In general, type of habitat affects species diversity of moths. Keyword : Diversity, moth and Gonoharjo forest.
PERTUMBUHAN ISOLAT RHIZOBAKTERI PELARUT FOSFAT DARI TANAMAN PADI DI MAYONG, JEPARA PADA MEDIA LIMBAH RUMAH PEMOTONGAN HEWAN DAN AIR KELAPA Rutty Wulandari; Agung Suprihadi; Budi Raharjo
Jurnal Akademika Biologi Vol. 2 No. 2 April 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

The farmers are now turning towards the use of biofertilizer. The biofertilizer is living microbes applied to the soil in order to help facilitate or provide certain nutrients for plants. Previous study found that B4 isolate has proven capable of solubilizing phosphate so that it could be used as an agent  that was inoculated in biofertilizer. The fertilizer can be formulated by modifying the alternative growth media in the liquid form that has potential, which is slaughterhouse waste and coconut water. Both  media contain organic matters which support the growth of bacteria. This study aimed to test the potential of slaughterhouse waste and coconut water as a medium for the growth of B4 isolate. Methods  of research conducted by Randomized Block Design, in which consists of five treatments namely P1 (100% coconut water), P2 (25% slaughterhouse waste & 75% coconut water), P3 (50% slaughterhouse waste & 50% coconut water), P4 (75% slaughterhouse waste & 25% coconut water), and P5 (100% slaughterhouse waste). The measured variable was the number of bacteria in the organic waste media during the 48 hours incubation period. Data on the number of bacteria was analysed by ANOVA  test  then  continued  with  Duncan  and  LSD  further  tests.  The  results  showed  that P4 treatment significantly different from other treatments, with the highest number of bacteria (1,9 x 1011CFU/mL) at 18 hours incubation. The density of bacterial population in the five formula medias every 3 hours during the 48 hours incubation period showed significantly different, except at 0, 3, 12, and 45 hours incubation. Key words: biofertilizer, phosphate, slaughterhouse waste, coconut water
KEANEKARAGAMAN JENIS LICHENES EPIFIT PADA HUTAN KOPI DAN HUTAN CAMPURAN DI NGLIMUT GONOHARJO KENDAL Senjha Mutiara Asih; Jumari J; Murningsih M
Jurnal Akademika Biologi Vol. 2 No. 2 April 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Wana Wisata Nglimut has the hilly of topograpy land and the high biodiversity. One of which them are many groups of Lichenes. This research aims was to determine the diversity of lichenes species and the diversity of thallus lichenes form in coffee plantation and mixture forest of Wana Wisata Nglimut Gonoharjo. The research was done in coffee forest and mixture forest of Wana Wisata Nglimut Gonoharjo, Kendal, Central Java. Lichenes sampling was done in coffee plantation and mixed forests of Wana Nglimut Gonoharjo, Kendal, Central Java. Sampling was done on by square plot, plot determination of the square plot using purposive method. Results of research on diversity lichenes in Wana Wisata Nglimut Gonoharjo Kendal undertaken in mixed forests and coffee plantation, obtained 42 species of lichenes. Nine species lichenes were found in the coffee plantation, and 41 Lichenes are found in mixed forest, and 8 species found in the coffee plantation and mixed forests. The results showed that the crustose type was the type of thallus lichenes that most commonly found in coffee forest whilest foliose type was lichenes that most commontly found in mixture forest.Keywords: Lichenes Epiphytes, Diversity, Wana Wisata Nglimut
UJI TOTAL GLUKOSA DAN AKTIVITAS ANTIOKSIDAN BEBERAPA PRODUK PANGAN FUNGSIONAL BERBAHAN DASAR UBI JALAR UNGU (Ipomoea batatas L.) var AYAMURASAKI Edysri Hermayudha P; Munifatul Izzati; Endang Saptiningsih
Jurnal Akademika Biologi Vol. 2 No. 2 April 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Functional food is food with high nutrition and important to illness terapy. Functional food product based of purple sweet potato are an alternative to replace primary food. This research purpose to know the quantity of total glucose, antioxydant activity, and antosianin decreaseful colour in a few of functional food product based of purple sweet potato. This research take place in food technology laboratoryum Unika Semarang on Desember until January 2013. This research use RAL single factor of food making process include milk, ice cream, wet noodle, dry noodle, and flour look for quantity of total glucose, antioxidant activity, and antosianin decreaseful colour. Method of research include analyse quantity of total glucose assay fenol method, antioxidant activity assay Diphenyl Pycril Hydriazil (DPPH), and observation in antosianin decreaseful colour product. The result of research show that the best food product is purple sweet potato flour. That product has quantity of total glucose 2,805 g/100 g, IC50  - 49951 %, and antosianin colour product take no damage because it still look purple. Keyword: the functionality food product, total glucose quantity, antioxydant activity IC50, antosianin decreaseful colour
STRUKTUR KOMUNITAS MIKROARTROPODA BRYOFAUNA TERESTRIAL DI TIGA KETINGGIAN YANG BERBEDA DI ZONA MONTANA GUNUNG UNGARAN Andrei Febrian; Rully Rahadian; Lilih Khotim Perwati
Jurnal Akademika Biologi Vol. 2 No. 2 April 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Bryofauna is the animal which live in mosses, including microarthtropod. Bryofauna microarthtropod is quite diverse but study about this fauna is rarely done, especially in Indonesia. Bryofauna has unique and specific niche, because it depends on the existence of mosses. The objective of this research is to compare the community structure of bryofauna microarthropod in three different altitude in montane zone, including: bryofauna diversity, abundance, richness and dominance. This research was conducted from April to December 2013. Sampling was conducted at three stations in different altitude, i.e. 1335 m asl, 1660 m asl and 2040 m asl. Sampling was done in purposive way, that means sampling was done only in location contained with mosses. Sampling used quadrat method, with size 1x1m, then the moss samples was taken on quadrat with size 10x10 cm in the 1x1 m quadrat. All samples was extracted using Tullgren Funnel. Bryofauna were identified in Ecology and Biosytematic Laboratorium, UNDIP dan Entomology Laboratorium Zoology Unit, LIPI. Bryofauna Microarthropod that found in montane zone were 7 class, 19 ordo, 44 sub-ordo/family. The dominants taxa on the montane zone are Oribatida and Mesostigmata. The highest bryofauna abundance were found in altitude of 2040 m asl. The highest bryofauna diversity and richnees was found in altitude of 1335 m asl. The highest bryofauna eveness were found in altitude of 1660 m asl. Bryofauna diversity is significantly different on each station. Differences in bryofauna microarthropod community strcuture were influenced by the presence of mosses at each altitude. Keywords : Community structure, bryofauna, microarthropod, montane zone

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