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Jurnal Akademika Biologi
Published by Universitas Diponegoro
ISSN : -     EISSN : 26219824     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Jurnal Akademika Biologi diterbitkan oleh Departemen Biologi Fakultas Sains dan Matematika Universitas Diponegoro Semarang. Jurnal ini sebagai media publikasi hasil karya ilmiah lulusan S1 Departemen Biologi. Jurnal Akademika Biologi menerima artikel-artikel yang berhubungan dengan bidang ilmu biologi.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 10 Documents
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Search results for , issue "Vol. 6 No. 4 Oktober 2017" : 10 Documents clear
KERAGAMAN SECARA MOLEKULER BAKTERI ASAM LAKTAT PADA ILEUM DAN SEKUM AYAM BROILER YANG DIBERI PERLAKUAN PAKAN HASIL FERMENTASI Chrysonilia crassa Husnul Khotimah; Siti Nur Jannah; Rejeki Siti Ferniah
Jurnal Akademika Biologi Vol. 6 No. 4 Oktober 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Lactic Acid Bacteria (LAB) are microflora in the digestive tract that has positive roles in poultry’s health. The amount and diversity of LAB in the gastrointestinal tract are influenced by several factors, one of them is by the feeding factor. The study aims to analyze the LAB diversity in ileum and cecum after feeding of fermented Chrysonilia crassa molecularly. Analysis of LAB spesies diversity is needed to provide a baseline profile of microbial community database on the illeum and cecum digestive tract of broiler chicken between control (commercial feed) and treatment (feed by Chrysonilia crassa fermentation) by the method of Terminal Restriction Fragment Length Polymorphism (T-RFLP). The calculated values are the number of phyilotypes, relative abundance, Shannon-Wiener (H') diversity index, evenness (E), and similarity. Group of LAB detected in the control were Lactobacillus delbrueckii (180 bp), Lactobacillus sp. (187 bp), Lactobacillus plantarum (572 bp), uncultured bacterium (87 bp) and unidentified (50 bp, 582 bp). The result of this study showed that by giving the fermented feed by Chrysonilia crassa had resulted in the decreasing of LAB diversity in ileum (0.66), cecum (0.48) compared with commercial feed (control) in ileum (0.84), cecum (1.05). Keywords: broiler chicken, cecum, ileum, LAB, T-RFLP
PENGARUH BERAT MEDIA DAN JUMLAH BIBIT TERHADAP PERTUMBUHAN DAN PRODUKSI KENTANG (Solanum tuberosum L.) DI DALAM POLYBAG Ulfah Ghina Fathiyyah; Munifatul Izzati; Sri Haryanti
Jurnal Akademika Biologi Vol. 6 No. 4 Oktober 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Potatoes (Solanum tuberosum L) are one of the plants that become source of carbohydrates that help strengthening food. Potatoes are an annual crop that are not environmentally friendly because the potatoes can accelerate the rate of erosion. Therefore, a cultivation method is needed in polybag. Planting in polybag need to know the weight of media and seeds number for growth and production of potatoes running optimally. The purpose of this study is examine potato cultivation in polybag to improving potato growth and production. Parameters observed were plant height, wet weight of potato, tuber number, tuber weight and tuber weight/polybag. The treatments were given are media weight (V7: 7kg and V10: 10 kg) planting potatoes in land as a control and number of seeds (2 grains: 3grains and 4grains). The treatment in this research do with factorial completely randomized design in 9 different treatment and 3 repeat. Data was analyzed by Analysis of Variance (ANOVA) at 95% confidence level and continued with the test of Duncan Multiple Range Test (DMRT). The result showed that the interaction between media weight and number of seeds had significant effect on wet weight of plant. but media weight factor treatment had significant effect on plant height, wet weight of plant, tuber number and tuber weight . The optimal result is weight media 10 kg with 2 seeds number. The weight treatment of 10kg media with the number seeds of 2 resulted in the maximum growth and production.. Keywords: Potatoes, polybag, media weight, seeds number.
PENGARUH PEMBERIAN LIMBAH KOPI TERHADAP PERTUMBUHAN TANAMAN SELADA (Lactuca sativa L.) Nimas Disri Putri; Endah Dwi Hastuti; Rini Budi Hastuti
Jurnal Akademika Biologi Vol. 6 No. 4 Oktober 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Lettuce plant is one of type of vegetable plant that has a high nutrient content consisting of protein, fat carbohydrates, calcium, and vitamins. Lettuce production in Indonesia has increased due to high demand of lettuce on the market. High production is supported by the high cultivation of lettuce. This high lettuce cultivation affects the use of fertilizer, where inorganic fertilizers are more widely used today. The use of inorganic fertilizers should be reduced because of its negative impact on the environment. Organic fertilizer is an appropriate solution to reduce the use of inorganic fertilizers. One of the materials that can be used as organic fertilizer is coffee waste.Coffee waste contains one of the essential nutrients in plant growth that is Nitrogen. This study aims to analyze that effect of solid and liquid coffee waste on the growth of lettuce plants (Lactuca sativa L.). The research was conducted by using Completely Randomized Design (RAL) with single factor that is the concentration of coffee waste. The study consist of 7 treatments, namely 0g/kg of coffe waste, 10g/kg of solid waste,20g/kg of solid waste, 30g/kg of solid waste, 10g/100ml of liquid waste, 20g/100ml of liquid waste, and 30g/100ml with 5 repetitions. The parameters observed were plant height, leaf number, wet weight and dry weight of plant, root length, wet weight and dry weight of root,. Data analysis using ANOVA followed by Duncan’s Multiple Range Test at 95% confidence level. The results showed that coffe waste significantly affected and improved the growth of lettuce (Lactuva sativa L.). Liquid waste concentration 10g/100ml gives the best result to the growth of lettuce plant (Lactuca sativa L.).   Key words: lettuce, Lactuca sativa L., coffe waste
ISOLASI DAN IDENTIFIKASI MOLEKULER KHAMIR DARI MOLASE SERTA KEMAMPUANNYA DALAM PRODUKSI ETANOL Fauziah Citra Rahmawati; Endang Kusdiyantini; Anto Budiharjo
Jurnal Akademika Biologi Vol. 6 No. 4 Oktober 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

The need of petroleum is increasing while the availability is limited. Petroleum is an alternative energy source which is produced by fermentation using a sugar-containing substrate with the help of yeast. This research aims to isolate the yeast that can ferment molasses into ethanol and identify morphologically, biochemically, and molecular so the species can be known. The isolation is done into the PDA media by streak method. Morphological identification involves macroscopic and microscopic observation. Biochemical identification involves growth tests on 50% glucose concentration and sugar fermentation. Molecular identification is done by looking at its genome based on Internal Transcribed Spacer (ITS) DNA base order. From Molasses isolation was obtained four isolates of yeast, that are isolates 1C1, 1C2, 2C1, and 3C2. Isolates 2C1 which is used for ethanol fermentation produces 0,9876 g/cm3 or 9,5% of ethanol. 2C1 Isolates was amplified using PCR and Phylogenetic Analysis using Neighbor Joining method. Sequencing results show that Isolates 2C1 has 616 bp Query Length. Based on Homology equation through BLAST method and phylogenetic tree analysis, isolates 2C1 is a Debaryomyces Hansenii.Keywords: Identification, Yeast, Molasses, Molecular, Ethanol
DETEKSI MRSA (Methicillin Resistant Staphylococcus aureus) PADA PASIEN RUMAH SAKIT DENGAN METODE MALDI-TOF MS DAN MULTIPLEX PCR Dian Wahyu Kemalaputri; Siti Nur Jannah; Anto Budiharjo
Jurnal Akademika Biologi Vol. 6 No. 4 Oktober 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Methicillin Resistant Staplylococcus aureus (MRSA) is a strain of Staphylococcus aureus which has been resistant to methicillin antibiotics and β-lactam group. The mechanism of MRSA resistance occurs because the Staphylococcus aureus produces Penicillin Binding Protein (PBP2a) encoded by the mecA gene that has low affinity to methicillin. Nowadays, MRSA is tested by resistance test using Oxacillin 1 ug. This method requires pure isolate and bacterial culture, so that the results can be seen in 5 days. To find a rapid and precise diagnostic technique for detecting MRSA, mecA gene detection utilizing PCR multiplex technique as the alternative diagnostics. This study aims to find a quick and precise alternative diagnostic technique for MRSA examination, which is utilizing MALDI-TOF MS and multiplex PCR technique.Keywords: MRSA, Staplylococcus aureus, MALDI-TOF MS, Multiplex PCR.
IDENTIFIKASI JENIS PIGMEN DAN UJI POTENSI ANTIOKSIDAN EKSTRAK PIGMEN BAKTERI Rhodococcus sp HASIL ISOLASI DARI SEDIMEN SUMBER AIR PANAS GEDONG SONGO Muhamad Fikri Zulfikar; Endang Kusdiyantini; Siti Nur Jannah
Jurnal Akademika Biologi Vol. 6 No. 4 Oktober 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Pigments are the coloring agents commonly used in the pharmaceutical, cosmetic, and food industries. This compound has the ability as antibacterial, anticancer, antibiotic and antioxidant. Pigment can be obtained synthetic and natural, natural pigments can be produced by plants, animals and microbes, one of which microbes is Rhodococcus sp. This study was conduct to identify the pigment found in Rhodococcus sp and measure the antioxidant activity of pigment produced by Rhodococcus sp. Growth and pigments production Rhodococcus sp was  grown on NB (Nutrient broth) medium with 96 hours incubation. This Growth was measured by dry weight cell. Identification of pigment using Thin Layer Chromatography and spectrophotometer UV-Vis. Measured of antioxidant activity Rhodococcus sp pigment using Carotene Bleaching methods. The results of pigments obtained from the identification by separation of pigment samples by thin layer chromatography resulted in 2 fractions on silica gel plate GF 250 with Rf values of 0.75 and 0.90. and analysis of UV-Vis spectrophotometers obtained optimum wavelengths 395 and 420 nm is beta-carotene pigments. Rhodococcus sp pigment measured its antioxidant activity with carotene bleaching method with the antioxidant activity value 51,24%.Keywords: Pigment, Rhodococcus sp, Pigment identification, antioxidant activity
PERILAKU MAKAN DAN MINUM PADA ANAK ITIK MAGELANG (Anas plathyrhynchos) PASCA TETAS DARI INDUK YANG DIBERI PERLAKUAN KURKUMIN, CAHAYA PUTIH DAN CAHAYA MERAH Fajar Sara Kusumawati; Silvana Tana; Enny Yusuf Wachidah Yuniwarti
Jurnal Akademika Biologi Vol. 6 No. 4 Oktober 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

This study aims to examine the effect of white light treatment, red light, curcumin with white light and curcumin with red light on the parent to the eating and drinking behavior of magelang ducks (Anas plathyrhynchos). This study uses 16 magelang ducklings consisting of 4 treatments. The observations in this study were from 4 groups namely A.1B1 which is the duckling of the original treatment given white light, A1B2 which is the duck of the parent treatment given red light, A2B1 which is the duck of the parent treatment given curcumin dose 18 mg / kg and white light, and A2B1 which is a duck from the parent treatment given curcumin dose 18 mg / kg and white light. Parameters measured in this study were data of eating behavior and data of drinking behavior of magelang ducks (Anas plathyrhynchos). This research is analyzed by descriptive analysis. The conclusions of this study are eating and drinking behavior in post-hatch children duck treated from the parent who was given a white light that is decreased the frequency of eating and drinking in the afternoon in the second week, eating and drinking behavior in post-hatch ducks after treatment of the mother given red light that is decreased the frequency of eating and drinking in the morning in the second week, eating and drinking behavior in post-tetas duck treated from the parent who was given curcumin and white light that decreased the frequency of eating in the afternoon while the frequency of drinking tends to decline throughout the day in the second week as well as eating and drinking behavior in post-tetas ducklings treated from the parent who was given curcumin and red light that increased the frequency of eating throughout the day at week II and decreased the frequency of drinking during the day on week II.Key words: feeding behavior, drinking behavior, magelang duck, curcumin, red light and white light
PRODUKSI ENZIM INULINASE Pichia manshurica DUCC-Y015 DENGAN PENAMBAHAN SUBSTRAT TEPUNG BENGKOANG (Paschyrhizus erosus) Adzar Rofiqoh; W Wijanarka; Susiana Purwantisari
Jurnal Akademika Biologi Vol. 6 No. 4 Oktober 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Bengkoang (Pachyrhizus erosus) tubers has a high inulin content. Inulin bengkoang flour can be used as substrate to produce inulinase enzyme. The inulinase enzyme can be produced by Pichia manshurica DUCC-Y015. This research aims to determine the ability of Pichia mansurica DUCC-Y015 in producing inulinase enzyme with the addition of several variations of substrate concentration of bengkoang flour in its production medium. Determination of inulinase activity was done by DNS method. This research used a Completely Randomized Design (RAL) with 4 treatments: B0 (control), B1 (1 g bengkoang flour), B2 (3 g bengkoang flour) and B3 (5 g bengkoang flour). Each treatment was repeated 3 times. The inulinase activity of each treatment was 0.029 IU/mL, 0.033 IU/mL, 0.053 IU/mL and 0.015 IU/mL. The addition of variation substrate concentration bengkoang flour in the production medium did not affect the inulinase activity of Pichia manshurica DUCC-Y015Kata Kunci: Pachyrhizus erosus, inulinase, Pichia manshurica DUCC-Y015.
UJI AKTIVITAS ENZIM FITASE YANG DIHASILKAN OLEH Aspergillus niger DAN Neurospora sp. PADA KONDISI FERMENTASI YANG BERBEDA Devi Ayu Ningsih; Endang Kusdiyantini; Budi Raharjo
Jurnal Akademika Biologi Vol. 6 No. 4 Oktober 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Phytase (E.C 3.1.3.8) is an important enzyme to overcome high phytic acid in livestock feed since this enzyme has the ability to hydrolyze phytic acid contained in feed material into inositol, glucose and organic phosphorus compound. This phytase enzyme can be produced by Aspergillus niger and Neurospora sp. The purpose of this study is to obtain the condition of fermentation by Aspergillus niger dan Neurospora sp. which is optimal in the production of phytase enzyme.The treatment of fermentation condition includes different pH, temperature and addition of C source (glucose). The production of enzyme was carried out in media containing glucose KCl, MgSO4.7H20, K2HPO4, NaOH with the addition of Palm Oil Mill Effluent. Mold was grown in liquid medium and measured the activity of phytase enzyme using spectophotometry. This study used a Complete Randomized Design factorial pattern with two factors. The first assaying ANOVA used a variation in the concentration of Palm Oil Mill Effluent with pH while, the second assaying used a variation in the concentration of Palm Oil Mill Effluent with temperature, and third assaying used a variation of glucose concentration with incubation times. Each factor was repeated 3 times. The data obtained were then analyzed using Analysis of Variance (ANOVA). The result of observation showed optimal phytase enzyme activity for A. Niger at pH 5 onthe temperature of 350C as much as 0.241 and 0.258 U U/ml while Neurospora sp. at pH 6 on the temperature of 350C as much as 0,273 and 0,253 U/ml. The addition of glucose experienced optimal phytase activity for Aspergillus niger as much as 0,099 U/ml at incubation time in 96 hours and Neurospora sp. as much as 0,152 U/ml in 48 hours will then decrease at subsequent incubation time.  Keywords: phytase, Palm Oil Mill Effluent, pH, A. niger and Neurospora sp.
PERILAKU BERTELUR DAN SIKLUS HIDUP Aedes aegypti PADA BERBAGAI MEDIA AIR Indira Agustin; Udi Tarwotjo; Rully Rahadian
Jurnal Akademika Biologi Vol. 6 No. 4 Oktober 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Aedes aegypti is the main vector that transmits dengue fever. The spread of Aedes aegypti mosquitos in Indonesia is very wide. This mosquito has its breeding site in clear water areas such as in the bath water containers, flower pots, pet containers as well as in the used goods that can hold stagnant water. However, the environmental conditions which are constantly changing due to the rampant pollution cause Ae. Aegypti to continue to adapt to its breeding environment. This research aims to find out the egg laying behavior, life cycle and mediums preferences selected by Aedes aegypti mosquitos in different water mediums. Stages of the research which are conducted include: preparation of research tools and materials, observation on Ae. Aegypti mosquitos’ preferences, observation on egg hatchability and life cycle of Ae. Aegypti in various water mediums. The result shows that from all contaminated water mediums which are tested, Ae. Aegypti mosquitos have the preference of laying eggs in Eichhornia crassipes water mediums, followed by leachate water mediums, whereas in tofu wastewater mediums and laundry waste, there were no eggs found. Meanwhile, in another study, it is known that the egg hatchability and life cycle of Ae. Aegypti mosquitos in contaminated water mediums such Eichhornia crassipes immersed water mediums, and leachate water are not significantly different from controlled water mediums (uncontaminated). The result of the research is expected to be the reference for relevant institute in expanding the eradication of Ae. Aegypti mosquitos to places that have been unusual for eradication.Keywords: Aedes aegypti, Eichhornia crassipes immersed water, Leachate, laundry waste, tofu wastewater

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