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Jurnal Akademika Biologi
Published by Universitas Diponegoro
ISSN : -     EISSN : 26219824     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Jurnal Akademika Biologi diterbitkan oleh Departemen Biologi Fakultas Sains dan Matematika Universitas Diponegoro Semarang. Jurnal ini sebagai media publikasi hasil karya ilmiah lulusan S1 Departemen Biologi. Jurnal Akademika Biologi menerima artikel-artikel yang berhubungan dengan bidang ilmu biologi.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 9 Documents
 1 
Search results for , issue "Vol. 7 No. 1 Januari 2018" : 9 Documents clear
HEPATOSOMATIC INDEX (HSI) DAN DIAMETER HEPATOSIT MENCIT (Mus musculus L.) SETELAH PAPARAN EKSTRAK AIR BIJI PEPAYA (Carica papaya L.) Perwitasari Wahyuningtyas; Agung Janika Sitasiwi; Siti Muflichatun Mardiati
Jurnal Akademika Biologi Vol. 7 No. 1 Januari 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Papaya seeds were one part of the papaya plants that can be used as a traditional medicine. The purpose of this study was to test the effect of papaya seed extract (Carica papaya L.) to Hepatosomatic Index (HSI) and hepatocytes diameter of mice (Mus musculus L.). This study was an experimental study use Completely Randomized Design (CRD) that divided into 5 treatment groups with 3 replications. P0 test material in the form of aquadest, P1, P2, P3 in the form of papaya seeds extract test with dose respectively 1.4; 3.5; And 7 mg / mL. Treatment were administered orally for 21 consecutive days. The Body weights of mice were measured every 7 days. After treatment were terminated, the body weights of mice were measured and then sacrificed by dislocation of the neck and the liver were taken by surgery. Furthermore, the liver weights were measured and made histological preparations with HE staining. The determination of HSI were obtained from liver weights and body weights. The measurement of hepatocytes diameter were done by using photomicrograph at each incision 3 field of view. Data were analyzed by Analysis of Variance (ANOVA) at 95% confidence level. The analysis result of HSI in the treatment group showed that there were no significant difference (p>0,05). The analisys of hepatocytes diameter of the treatment group showed significant difference (p<0.05). It could be concluded that exposure of papaya seeds extract for 21 days was a reversible effects so that the hepatocytes diameter size of mice increases but didn’t changed the HSI.Keywords: Carica papaya, HSI, hepatocytes diameter
ISOLASI DAN IDENTIFIKASI MOLEKULER BAKTERI ANTAGONIS TERHADAP Vibrio parahaemolyticus PATOGEN PADA UDANG Litopenaeus vannamei DARI PRODUK PROBIOTIK DAN SEDIMEN MANGROVE DI REMBANG Bunga Fajriani; Anto Budiharjo; Sri pujiyanto
Jurnal Akademika Biologi Vol. 7 No. 1 Januari 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Shrimp is one of the main commodities in the aquaculture industry because it has high economic value and high demand product. The Litopenaeus vannamei shrimp have the advantage of being able to grow as fast as a tiger shrimp (3 g / wk), can be grown on a wide salinity range (0.5-45 ppt), lower protein requirement (20-35%) than tiger shrimp and stylirostris shrimp. Vibrio parahaemolyticus is a normal flora in the brackish waters environment which is pathogenic to shrimp commodities as well as in humans. The use of Probiotics as additional feed in the form of microbial cells intact widely used in shrimp farming as one effort to improve the quality of the environment and supress the growth of pathogenic bacteria. One of the probiotic products used is super PS. This study aims to obtain bacterial isolate probiotic products that can suppress the growth of Vibrio parahaemolyticus bacteria. Isolation is also done on mangrove sediments as a comparable type of bacteria that can suppress the growth of pathogens. Methods used include isolation of bacterial probiotic and mangrove sediments, antagonistic test, and molecular identification with PCR methods (Polymerase Chain Reaction). The isolation result obtained by seven isolate bacteria of probiotic product and eleven isolate of mangrove sediment bacteria. One selected bacterial isolate from isolation of probiotic product that is IP 7 which able to supress the growth of Vibrio parahaemolyticus with a diameter of clear zone 10.84 mm. The results of identification using the 16S rRNA gene sequence showed that IP 7 isolate had a similarity index of 92% with Lysinibacilllus cresolivorans.Keyword : Litopenaeus vannamei, Vibrio parahaemolyticus, probiotics, antibacterial, gen 16S rRNA.
KARAKTERISTIK MORFOLOGI, BIOKIMIA, DAN MOLEKULER ISOLAT KHAMIR IK-2 HASIL ISOLASI DARI JUS BUAH SIRSAK (Annona muricata L.) Vivi Suryaningsih; Rejeki Siti Ferniah; Endang Kusdiyantini
Jurnal Akademika Biologi Vol. 7 No. 1 Januari 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Yeast was found in foods that contain lots of sugar as fruits. Yeast utilizes simple sugars in food to gain energy. Yeast has a role in the natural fermentation of the fruit that resulting of decay, discoloration, and cause the fruit not durable. The purpose of this research were to isolate the yeast from the soursop fruit and to identify the yeast in morphology, biochemistry, and molecular test based on Internal Transcribed Spacer (ITS). Isolation of  yeast  was  performed by growing on  medium PDA and chloramphenicol. Identification was done through biochemical test by looking at the capabilities in the fermentation of carbohydrate and the abilities to grow on a medium to high osmotic pressure. Molecular identification was done using Internal Transcribed Spacer (ITS) region. The result showed that isolate IK-2 yeast from the soursop fruit juice had a distinctive form round to oval, prominent elevation, the colour creamy white, form a buds, able to ferment glucose and sucrose, but not able to ferment lactose, as well as being able to grow on media with glucose level 50 %. Molecular analysis of the ITS region using ITS1 and ITS4 primers, and phylogenetic analysis using Neighbor Joining. The result of the Basic Local Alignment Tools (BLAST) showed that the isolate had 95% homology with Candida tropicalis. Key Words : Yeast, Soursop (Annona muricata L.), Internal Transcribed Spacer (ITS)
PRODUKSI INULINASE DARI UMBI DAHLIA (Dahlia variabilis) OLEH Pichia manshurica DUCC Y-015 DENGAN VARIASI WAKTU INKUBASI DAN KONSENTRASI GLUKOSA SEBAGAI SUMBER KARBON TAMBAHAN Fathika Fitrania; MG. Isworo Rukmi; w wijanarka
Jurnal Akademika Biologi Vol. 7 No. 1 Januari 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

The production of fructose from inulin by inulinase would only take one stage enzimatis reaction and yielding 95% fructose. Inulin obtained from the dahlia tubers and inulinase produced by Pichia manshurica DUCC Y-015. The production of inulinase  (E.C 3.2.1.7) can be influenced by the presence of glucose as  additional carbon source. The purpose of this research is to analyze influence of  variation glucose concentration and incubation time to production inulinase P. manshurica DUCC Y-015 in dahlia tubers substrate. Measurement of production enzyme covering activity inulinase and activity invertase. Both analyzed by DNS method and determined based on 1 µmole fructose resulting. The design used in this research were Randomized Factorial Block Design ( RAFBD ). Factor I in the form of glucose concentration 0% (G0); 0.25% (G1); 0.5% (G2) and factor II were incubation time 6 hours (T6), 12 hours (T12), and 18 hours (T18) with repetition 3 times. The data obtained were analyzed by ANOVA method. The result showed variation of glucose concentration and incubation time difference had no significant effect on production of inulinase from P. manshurica DUCC Y-015. The highest production of the inulinase was demonstrated by treatment G1T12 with  0,25% glucose and incubation time 12 hours with value of the activity of inulinase as much as 0,574 IU/mL. Keywords : Dahlia tubers,  Glucose, Inulinase, Incubation time, Pichia manshurica DUCC Y-015,
TINGKAH LAKU MAKAN DAN MINUM ITIK MAGELANG (Anas javanica) SETELAH PEMBERIAN CAHAYA MERAH DAN PUTIH SERTA KURKUMIN DALAM PAKAN Martha Raras Vegasari; Siti Muflichatun Mardiati; Enny Yusuf Wachidah Yuniwarti
Jurnal Akademika Biologi Vol. 7 No. 1 Januari 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Magelang duck was one of the most productive local poultry as an egg producer. Improvements in the management of aves cultivation could be done with the addition of curcumin and light. Curcumin and light were able to affect the behavior of feeding and drinking. This study aimed to analyze the increases of feeding and drinking behavior of magelang duck (Anas javanica) which was fed with curcumin with red and white lighting. This study used 12 female ducks that were 17 weeks old. The study consisted of 4 groups: A0B0 (white light and curcumin 0 mg), A0B1 (white light and curcumin 18 mg), A1B0 (red light and curcumin 0 mg), and A1B1 (red light and curcumin 18 mg). The parameters observed in this study were the frequency of feeding and drinking of magelang ducks (Anas javanica). This research was analyzed by descriptive method. The conclusion of the study was that the giving of red light and white light and curcumin in the curcumin has not potential to improve the duck feeding activity in the stationary phase, while giving white light has potential to improve the behavior of drinking of magelang ducks.Key words: feeding and drinking behavior, Magelang duck, curcumin, red and white light.
KEMAMPUAN AKUMULASI TIMBAL (Pb) PADA AKAR MANGROVE JENIS Avicennia marina (Forsk.) DAN Rhizophora mucronata (Lamk.) DI LAHAN TAMBAK MANGUNHARJO SEMARANG Titi Wulandari; Rini Budi Hastuti; Endah Dwi Hastuti
Jurnal Akademika Biologi Vol. 7 No. 1 Januari 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

The waters in Mangunharjo are close to industrial activities and human settlements. The high activity in the Mangunharjo area, can flow a variety of waste that can cause pollution, one of which pollution Heavy Metal Pb. This study aims to analyze the ability of roots and leaves Rhizophora mucronata and Avicennia marina in accumulating heavy metal pollutant Lead (Pb). The research was conducted in the pond area of Mangunharjo Subdistrict, Semarang. The design of this research using Randomized Completely Randomized Design 2x1: Factor I namely Type (Avicennia marina and Rhizophora mucronata) at tree stage, Factor II that is root organ with two sampling within 30 days. The research parameters included Pb metal content on mangrove root, and Pb metal content in water and sediment. The result of this research can know the level of Pb metal in water and the environmental sediments of Avicennia marina are 0.36-0,9475 mg / l and 0,941-1,455 mg / kg, respectively. The concentration of Pb metal in water and sediment at the root of Rhizhophora mucronata was 0.175-0,243 mg / l, respectively. Types and Organs do not interact because of their significance of 0.732 (> 0.05). The ability of mangroves to absorb heavy metals in aquatic environments can be determined by calculating Bio Concentration Factors (BCF). BCF values for mangroves Avicennia marina and Rhizophora mucronata ranged from 0.0566 to 0.1068, with the highest values at the root Rhizophora mucronata (0.2879-0.0638). Keywords: Accumulation, Avicennia marina, Rhizophora mucronata, Bio Concentration Factor, significance
AKTIVITAS ENZYM SELULASE YANG DIHASILKAN OLEH BAKTERI Serratia marcescens PADA SUBSTRAT JERAMI Khrisna Lazuardi Budi; W Wijanarka; Endang Kusdiyantini
Jurnal Akademika Biologi Vol. 7 No. 1 Januari 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Cellulose (EC 3.2.1.4) is enzyme complex consisting of some enzymes which together decomposing cellulose into glucose by hydrolizes the β-1,4 bond in cellulose. The purpose of this study is to determine cellulose activity which produced by Serratia marcescens in different substrate concentration and at the time of incubation T4, T8, T12. This research uses Randomized Block Design (RBD) factorial pattern with two factors. The first factor was variation of straw substrate which had been delignificated (V0, V1, V2, V3). The second factor is the variation of time incubation (T4, T8, T12). Each factor is repeated 3 times. The data obtained were analyzed using Analysis  of  Variance (ANOVA)  (α  =  0.05). The result  shown that  variation concentration  of straw,  and the interaction (combination) between the straw substrate and the incubation time substrate was not significantly different. The result treatment of incubation time was significantly different of the cellulase activity. The result of anova analyzed is obtained that F count(α = 0.05) value from straw substrate, interaction (combination) between the straw substrate and the incubation time substrate, and incubation time was 0.53; 2.18; 8.00. F table(α = 0.05) value of straw substrate, interaction (combination) between the straw substrate and the incubation time substrate, and incubation time was 2.99; 2.20; 3.39. The result of anova, is continued by BNT 5% test. The result of BNT test shown that the highest incubation time of cellulase activity was in incubation time 12 hours with the average value 0.26 U/mL. Key Word : cellulose,  Serratia marcescens,  straw substrate, incubation time
RESPON PEMBERIAN PUPUK UREA DAN PUPUK KANDANG SAPI TERHADAP PERTUMBUHAN DAN KANDUNGAN MINYAK ATSIRI TANAMAN JAHE MERAH [Zingiber officinale (L.) Rosc var. rubrum ] Linda Saputri; Endah Dwi Hastuti; Rini Budi Hastuti
Jurnal Akademika Biologi Vol. 7 No. 1 Januari 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Red Ginger plant is one of the export commodities and has benefits as perfume industry, cosmetics, health, and spices. Currently, the volume of demand for ginger products continues to increase, but the demand has not been met because of low productivity so that the need for intensification and provision of fertilizer. The purpose of this research is to study the effect of urea and cow manure on the growth of red ginger plants and to study the type of urea fertilizer and cow manure that gives maximum effect to growth and the essential oil content of Ginger Red plant. Observed parameter include plant height, leaves numbers, plant wet weight, and essential oil content. The study used Completely Randomized Design (RAL) with 4 treatments and 3 replications. Intended treatments are without fertilizer, with cow manure, with urea fertilizer, and with combination of urea fertilizer and cow manure. Data were analyzed with Analysis of Variance (ANOVA) at 95% significant level. Red ginger plants gave cow manure 150 g / polybags tend to produce plant height, plant wet weight, and higher essential oil content. Key Words : Growth, red ginger, urea fertilizer, cow manure
ISOLASI, UJI AKTIVITAS, DAN OPTIMASI INHIBITOR α-AMILASE ISOLAT KAPANG ENDOFIT TANAMAN BINAHONG (Anredera cordifolia) (Ten.) Steenis Diani Ajeng Prahesti; Sri Pujiyanto; MG Isworo Rukmi
Jurnal Akademika Biologi Vol. 7 No. 1 Januari 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Diabetes mellitus (DM) is a hyperglycemia disease caused by insensitivity of cells to insulin that causes the presence of glucose in blood and urine. The α-amylase inhibitors is a compound that inhibit carbohydrates breakdown into glucose by amylase enzyme. Binahong (Anredera cordifolia) has been used by Indonesian people since long time ago to treat various diseases such as for anti-diabetic. The aims of study were to obtained the endophytic fungal isolated from Binahong plant, and find out of their ability of α-amylase inhibitor activity by treatment of carbon source and pH of production medium. The isolating endophytic fungal from binahong roots, stems, and leaves in medium PDA. The α-amylase inhibitory test was using starch substrate and the α-amylase inhibitory measure using spectrophotometric method. The study was used one factor CRD i.e. carbon source including sucrose, lactose, and maltose. The best carbon source then continued to test with variation pH 5,6, and 7. The data obtained were then analyzed by Analysis of Variance (ANOVA). Five endophytic fungal isolated from binahong were only one isolated i.e DT should high potensial in inhibiting α-amylase 91,43%. The best carbon source for inhibitory α-amylase i.e sucrose with the percentage of 58,91%, while pH 5 is the best pH medium production continous treatment carbon source sucrose with the percentage 37,83%. The result of statistical test showed that sucrose as the best carbon source just than lactose and maltose, and pH 5 as the best medium pH for the production α-amylase inhibitor. Key words : α-amylase inhibitor, endophyte fungi, Anredera cordifolia, diabetes mellitus

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