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Journal of Biotropical Research and Nature Technology
Published by Pandawa Institute
ISSN : 29640431     EISSN : -     DOI : https://doi.org/10.47352/jbrnt.2964-0431
Core Subject :
Focus and Scope Journal of Biotropical Research and Nature Technology (J.Biotropica. Res. Nat. Technol.) is an open access, peer-reviewed journal dedicated to the publication of novel research concerned with the advancement of Biodiversity studies of Wetland tropical. It publishes original research articles, short communication, reviews or results of case study and projects base learning (PJBL) on the following subjects: Studies on Biodiversity topics such as: biodiversity patterns, conservation priority, wildlife management, conservation plan and ecosystem functioning under global change in Asia. Studies on Zoological topics such as: Animal Behavior, Comparative Physiology, Evolution, Ecology, Systematics and Biogeography. Studies on Botanical topics such as: plant genetics, biochemistry, cell biology, molecular biology, plant physiology and biosystematics. This includes comprehensive series of volumes dealing with the genome evolution of plants. Recent developments on plant metabolic. Studies Potential Biology topics such as: exploration of the applicable of probiotics, antimicrobials including antibacterial, antiviral, antifungal, antiprotozoal agents, and their derivatives in biomedical, agricultural, food, and cosmetic products. Manuscripts that are not in accordance with the subjects will not be considered to be published. We also discourage manuscripts written by a single author.
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Articles 59 Documents
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Phylogenetic and genetic diversity of marble goby (Oxyeleotris marmorata) from the Brantas River, East Java, Indonesia Wahyu Endra Kusuma; Bintang Rhafsyanjani; Dina Faizatin; Lunaya Zahrani; Ni Putu Anggun Larasati; Elok Amalia; Ifa Sufaichusan; Muhammad Dailami; Muhammad Nurfa’iq Hibatullah; Kiki Nur Azam Kholil
Journal of Biotropical Research and Nature Technology Vol. 5 No. 1 (2026): Journal of Biotropical Research and Nature Technology
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jbrnt.2964-0431.437

Abstract

The marble goby (Oxyeleotris marmorata) is a commercially important freshwater fish in Southeast Asia with considerable potential for aquaculture development. However, information regarding its genetic diversity and phylogenetic relationships remains limited, particularly in Indonesia. This study aimed to assess the genetic diversity and phylogenetic relationships of O. marmorata populations from the Brantas River, East Java, using mitochondrial cytochrome c oxidase subunit I (COI) sequences. Specimens were collected from Malang and Kediri, and the resulting sequences were analysed together with reference sequences obtained from GenBank. Genetic diversity was evaluated using haplotype diversity (Hd), nucleotide diversity (π), haplotype network reconstruction, genetic distance analysis, and phylogenetic inference. A total of five haplotypes (H1-H5) were identified from 37 sequences. Haplotype H1 was the most common and widespread haplotype, occurring in all Indonesian populations as well as in Thailand and Cambodia. In contrast, H2 was unique to Vietnam, whereas H3-H5 were restricted to Thailand. All individuals from the Brantas River shared a single haplotype, resulting in zero haplotype diversity (Hd = 0) and nucleotide diversity (π = 0). Phylogenetic and haplotype network analyses consistently recovered two mitochondrial lineages, while genetic distances among haplotypes ranged from 0.16% to 1.69%, indicating shallow intraspecific divergence. The absence of mitochondrial variation in the Brantas River populations may reflect historical population connectivity, reduced mitochondrial variability, or past demographic events such as population bottlenecks. These findings provide baseline genetic information to support future conservation, broodstock management, and domestication programs for O. marmorata.
Linking Untargeted Metabolomics to Functional Bioactivity in Nutmeg (Myristica fragrans Houtt.): Current Advances, Bioactivity Integration, and Analytical Challenges Susilo Susilo; Ratna Yuniarti; Titin Siswantining; Yasman Yasman
Journal of Biotropical Research and Nature Technology Vol. 5 No. 1 (2026): Journal of Biotropical Research and Nature Technology
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jbrnt.2964-0431.449

Abstract

Myristica fragrans Houtt. has been widely explored due to its high chemical composition and many functional features, nevertheless, the analytical framework applied to analyze this species has progressed unevenly. This study critically discusses the shift from conventional GC–MS-based phytochemical profiling to more integrated metabolomics techniques incorporating LC-HRMS, chemometrics, and bioactivity analysis in nutmeg research. A literature study was undertaken in five scientific databases using PRISMA guidance. Studies were categorized as MAIN and SUPPORT to distinguish between metabolomics-driven studies and studies providing complementary evidence of phytochemical and bioactivity data. The synthesis illustrates that most of the published work still depends on GC–MS analysis of volatile fractions and essential oils, but fully untargeted metabolomics investigations are rare. Recent developments have increased metabolite coverage, sample discrimination and helped identification of potential markers related to bioactivity interpretation, species authentication and quality assessment. Most studies still tend to report relationships between metabolites and bioactivity mostly as compositional or statistical associations, biomarker verification remains patchy across the literature and metabolite annotation is still often limited to MSI Level 2 or 3, although examples of higher-confidence identification are emerging. In sum, contemporary M. fragrans research can best be characterized as a shift from extended phytochemical profiling to more biologically interpretable metabolomics. Future advances will depend on increased standardization, more confident metabolite annotation, stricter chemometric validation, and wider integration of chemical signatures with functional biological evidence to enable more robust and translatable applications of nutmeg metabolomics.
Foliose Lichen Diversity and Substrate Occurrence in Bukit Baranahu, Bukit Tangkiling Nature Park, Central Kalimantan, Indonesia Risna Yanti Pasang Lolok; Vinsen Willi Wardhana; Frans Grovy Naibaho; Widya Krestina; Desimaria Panjaitan
Journal of Biotropical Research and Nature Technology Vol. 5 No. 1 (2026): Journal of Biotropical Research and Nature Technology
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jbrnt.2964-0431.450

Abstract

Lichen diversity in Indonesia remains unevenly documented, with Kalimantan still underrepresented in current lichenological records. Bukit Baranahu, located within Bukit Tangkiling Nature Park, Central Kalimantan, is a lowland conservation area with socio-ecological importances, but information on its biodiversity, including foliose lichens, remains unavailable. This study aimed to identify foliose lichen taxa and describe their substrate occurrence in Bukit Baranahu. Field collection was conducted from May to October 2023 using an exploratory cruise survey combined with purposive sampling along accessible paths from the foothill toward the upper part of the hill. Specimens were examined based on morphological, anatomical, and chemical characters, including standard spot-test reactions. A total of 22 foliose lichen specimens were recorded and assigned to 17 taxa belonging to Caliciales and Lecanorales. Caliciaceae was the richest family, mainly represented by Dirinaria, whereas Parmeliaceae and Physciaceae were represented by fewer taxa. This composition differed from several Indonesian lichen inventories in which Parmeliaceae was commonly reported as a dominant family. Foliose lichens were recorded on living trees, snags or dead standing wood, fallen logs, and rocks, with living trees contributing the highest proportion of substrate records. Several taxa occurred on larger-diameter trees and snags, suggesting that substrate size, bark condition, and substrate stability may support local foliose lichen establishment. These findings provide the first baseline account of foliose lichen diversity and substrate occurrence in Bukit Baranahu and contribute to lichenological documentation in the underexplored lowland forests of Central Kalimantan.
Accumulation of Manganese (Mn) in Tilapia (Oreochromis mossambicus) and Environmental Health Risk Assessment Mia Azizah; Muhamad Abdul Govin; Nina Ariesta
Journal of Biotropical Research and Nature Technology Vol. 5 No. 1 (2026): Journal of Biotropical Research and Nature Technology
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jbrnt.2964-0431.451

Abstract

Situ Tlajung Hilir in Gunung Putri District, Bogor Regency, has been reported to be polluted by industrial waste, which may cause heavy-metal accumulation in tilapia (Oreochromis mossambicus) consumed by the surrounding community. This study aimed to analyze the manganese (Mn) concentration in tilapia and the health risk to people consuming tu sing the Environmental Health Risk Assessment (EHRA) method. Fish samples were collected from three sampling points (A, B, and C) by purposive sampling, while anthropometric and consumption data were obtained through interviews with 30 respondents. Mn concentration was determined using Atomic Absorption Spectrophotometry (AAS) at a wavelength of 279.5 nm following wet digestion. The results showed Mn concentrations of 18.22–18.37 mg/kg (point A), 12.60–12.75 mg/kg (point B), and 6.25–6.29 mg/kg (point C), all exceeding the WHO 2004 standard of 1 mg/kg. The Risk Quotient (RQ) values ranged from 0.0187 (lowest) and 0.0645 (average) to 0.3116 (highest). Because all RQ values were < 1, the community of Situ Tlajung Hilir is still considered safe to consume tilapia under the exposure assumptions applied for up to 30 years ahead; however, these values may change if the rate and frequency of consumption increase.
Bioaccumulation of Heavy Metals Fe, Cu, and Mn in Mozambique Tilapia (Oreochromis mossambicus) from Situ Tlajung Hilir, Gunung Putri, Bogor Widya Kartika Laksmawati; Mia Azizah; Nurlela Nurlela; Sufrizal Ihsan Latief
Journal of Biotropical Research and Nature Technology Vol. 5 No. 1 (2026): Journal of Biotropical Research and Nature Technology
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jbrnt.2964-0431.452

Abstract

Freshwater ecosystems located near urban, industrial, and residential areas are vulnerable to heavy metal contamination derived from anthropogenic activities, including industrial discharge, domestic wastewater, and surface runoff. These contaminants may accumulate in aquatic organisms and potentially affect food safety when contaminated fish are consumed. This study evaluated the concentrations of iron (Fe), copper (Cu), and manganese (Mn) in the edible muscle tissue of Mozambique tilapia (Oreochromis mossambicus) collected from Situ Tlajung Hilir, Bogor Regency, Indonesia. Fish samples were obtained from three purposively selected locations representing areas influenced by community activities, industrial operations, and domestic wastewater discharge. Heavy metal concentrations were determined using Atomic Absorption Spectroscopy (AAS) following wet digestion of dried muscle tissues. The concentrations of Fe, Cu, and Mn varied among sampling locations, with Fe showing the highest accumulation, followed by Cu and Mn. Based on dry weight measurements, Fe concentrations ranged from 66.14 to 621.39 mg kg⁻¹, Cu from 0.87 to 21.27 mg kg⁻¹, and Mn from 5.14 to 6.18 mg kg⁻¹. Although numerical differences were observed among sampling locations, statistical analysis indicated that these differences were not significant, suggesting high within-site variability and limited statistical power due to the small number of biological replicates. The elevated concentrations of metals in fish muscle indicate that O. mossambicus from Situ Tlajung Hilir has accumulated Fe, Cu, and Mn, possibly reflecting the influence of surrounding anthropogenic activities. However, direct identification of contamination sources requires further analysis of water, sediment, and physicochemical parameters. These findings provide preliminary evidence of heavy metal bioaccumulation in fish from an urban freshwater ecosystem and highlight the need for continuous environmental monitoring and future human health risk assessment.
Diversity of Fabaceae in the Universitas Palangka Raya Area, Central Kalimantan, Indonesia Ria Windi Lestari; Yuli Wulan Sari; Umi Novita Fitriah; Fadhila Aziz; Decenly Decenly
Journal of Biotropical Research and Nature Technology Vol. 5 No. 1 (2026): Journal of Biotropical Research and Nature Technology
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jbrnt.2964-0431.453

Abstract

The Fabaceae family (Leguminosae), belonging to the order Fabales, comprises approximately 630 genera and 18,000 species distributed worldwide. According to the modern phylogenetic classification proposed by the Legume Phylogeny Working Group (LPWG), Fabaceae is divided into six subfamilies: Cercidoideae, Detarioideae, Duparquetioideae, Dialioideae, Caesalpinioideae, and Faboideae. Members of this family include herbs, shrubs, and trees, with a wide range of ecological adaptations in tropical ecosystems. This study was conducted within the campus area of Universitas Palangka Raya to document the diversity of Fabaceae species, which has not been comprehensively recorded in this region. A field survey method was applied to collect and identify specimens based on morphological characteristics. A total of 18 Fabaceae species were recorded. Caesalpinioideae comprised 11 species, Faboideae 6 species, and Detarioideae 1 species. Growth forms included herbs, shrubs, and trees. Based on ecological status, 6 species were classified as native and 12 as introduced according to Plants of the World Online (POWO). This study provides baseline floristic data on Fabaceae diversity in the Universitas Palangka Raya area and contributes to taxonomic and biodiversity documentation in Central Kalimantan.
Physicochemical Characterization and Water Quality Feasibility across Three Source Water Types in the Ciputat Area, South Tangerang Vetty Megantari; Nurul Amilia; Nita Rosita; Wahyu Permata; Fitriah Hatiningsih
Journal of Biotropical Research and Nature Technology Vol. 5 No. 1 (2026): Journal of Biotropical Research and Nature Technology
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jbrnt.2964-0431.456

Abstract

This study aims to characterize the physicochemical quality and assess the water quality feasibility across three source Water Source Type used in parallel within the Ciputat area, South Tangerang, namely tap water from public facilities and residential areas (Type I, n = 4), tap water from university buildings at Universitas Islam Negeri Syarif Hidayatullah Jakarta (Type II, n = 8), and drinking water from refill stations (Type III, n = 8), evaluated against the Ministry of Health Regulation (Permenkes) No. 2 of 2023. In situ parameters included pH, temperature, dissolved oxygen (DO), turbidity, total dissolved solids (TDS), and salinity. Laboratory analyses encompassed iron (Fe) and manganese (Mn) determination via atomic absorption spectrophotometry (AAS) across all three Water Source Types, as well as ammonia (N-NH3) and phosphate (P-PO4) analysis using UV-Vis spectrophotometry specifically for Water Source Types II and III. The results indicated that the turbidity of all samples (3.70–23.81 NTU) exceeded the maximum permissible limit of less than 3 NTU across all three water sources. Whereas, based on the WHO guideline, drinking water turbidity should not exceed 5 NTU [1]. The campus tap water was dominated by acidic pH levels (5.40–7.50), with six out of eight buildings falling below the minimum threshold of 6.5. Iron levels in all samples were below the method detection limit, whereas manganese levels exceeded the quality standard of 0.1 mg/L exclusively in Water Source Type I (0.336–0.853 mg/L), which is consistent with geogenic sources in shallow groundwater. The N-NH3 and P-PO4 concentrations in all samples complied with the specific parameter standards of Permenkes No. 2 of 2023. In conclusion, no matrix satisfied all parameters simultaneously. Hence, corrective interventions must be tailored to the dominant characteristics of each respective water source.
Interaction Modeling of Red Ginger (Zingiber officinale Var. rubrum) in Ruminant Feed Wafer as Antihypertension Through in Silico Luluatul Fajriyah; Erlix Rakhmad Purnama
Journal of Biotropical Research and Nature Technology Vol. 5 No. 1 (2026): Journal of Biotropical Research and Nature Technology
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jbrnt.2964-0431.467

Abstract

The increasing demand for animal products requires the development of feed innovations that not only meet nutritional needs but also improve animal health. Red ginger (Zingiber officinale Var. rubrum) is known as a feed ingredient rich in nutrients and bioactive compounds. The content of secondary metabolites such as flavonoids, alkaloids, terpenoids, phenols, and essential oils has the potential to provide therapeutic effects against several diseases, one of which is hypertension. Hypertension is known as high blood pressure in a condition above the normal threshold (>140/90 mmHg) which can affect the performance of the circulatory system in ruminants, such as environmental stress, an unbalanced diet, and other metabolic disorders. Thus, it can cause a decrease in metabolic efficiency, organ dysfunction, and even death. This study aims to evaluate the interaction potential of red ginger (Zingiber officinale Var. rubrum) bioactive compounds against the ACE receptor through an in silico molecular docking approach using captopril as a reference ligand. The initial step involved exploring the bioactive compounds in red ginger, compound structure identification, Lipinski’s druglikeness test, docking, and visualization. The results showed that of the seven compounds in red ginger, when compared to captopril (control) as an antihypertensive bioactive compound candidate of -5.7 kcal/mol, the α -Cur cumene or L4 had the lowest binding affinity value compared to the other ligands at -6.8 kcal/mol when interacting with the ACE protein, thus having the potential to be further developed as a bioactive compound candidate antihypertensive in ruminant animal feed, thus indicating its potential as a candidate ACE inhibitory compound for further computational and experimental investigation.
Comparative Study of Water Chemical Quality in the Air Telang Protected Mangrove Forest During the West and East Monsoons Dewi Rosanti; Indra Yustian; Sarno Sarno; Syaiful Eddy
Journal of Biotropical Research and Nature Technology Vol. 5 No. 1 (2026): Journal of Biotropical Research and Nature Technology
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jbrnt.2964-0431.468

Abstract

A study on the differences in water chemical quality in the mangrove area of ​​the Air Telang Protection Forest during the western and eastern rainy seasons was conducted from October 2024 (eastern monsoon) to April 2025 (western monsoon). The aim was to compare the water chemical quality, namely BOD, COD, DO, and pH in the area during the western and eastern monsoons, which support aquatic biota that depend on mangrove forests as spawning, feeding, and nursery grounds. This study used a survey method, with sampling carried out at five stations in different vegetation zones, namely the emerging sand (station 1), Sonneratia zone (station 2), Avicennia zone (station 3), Nipah degraded zone (station 4), and Rhizophora zone (station 5). The parameters observed were BOD, COD, DO, and pH. Samples were taken 3 times at each station. Based on the discussion above, it can be concluded that water quality during the east monsoon is better than during the west monsoon, based on chemical parameters such as BOD, COD, DO, and pH. However, during the west monsoon, only pH is tolerable for aquatic biota. This can be used as a basis for environmental management around mangrove ecosystems for the conservation of aquatic biota, as part of silvofishery (aquaculture) development.

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