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Biodiversitas Journal of Biological Diversity
Published by Universitas Sebelas Maret
ISSN : 1412033X     EISSN : 20854722     DOI : https://doi.org/10.13057/biodiv/d230231
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was accredited by DGHE, Ministry of Education, R.I., since 2014 was indexed by Scopus, where DOAJ and Google Scholar was indexed first.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 9 Documents
 1 
Search results for , issue "vol. 10 no. 2 (2009)" : 9 Documents clear
Partial Characterization of a Novel Reovirus Isolated from a Hypovirulent Strain (9B21) of Cryphonectria parasitica (Murrill) Barr SUPYANI SUPYANI
Biodiversitas Journal of Biological Diversity Vol. 10 No. 2 (2009)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d100203

Abstract

A filamentous fungus Cryphonectria parasitica (Murrill) Barr. is the causal agent of the chestnut blight disease. This fungus has some hypovirulent strains. These strains are known to be infected by viruses. To date, many species of viruses havebeen found to be associated with C. parasitica strains. The number genome segments, their relative size, titer and sequence homology as well as the effects on host fungal virulence and morphology were varied among the C. parasiticaassociating viruses. Cryphonectria parasitica mycoreovirus-1 (CpMYRV1) is a newly characterized reovirus isolated from C.parasitica. The viral genome consists of 11 segments of dsRNA termed S1 to S11. The aims of this research were to determine the complete sequences of the three shortest segments (S9-S11) of CpMYRV1, and to analyze the relationship of the dsRNA segments to the segments within the reoviruses genome. Sequence analyses showed that segments S9, S10, and S11 are 1072 bp, 975 bp, and 732 bp in size with single ORFs coding for 297, 247, and 101 amino acids respectively. Genome segments S9 to S11 have common terminal sequences, 5’GAUCA--- GCAGUCA3’. The deduced amino acid sequence coded by segment 9 showed a similarity to P11 of evolutionarily related Rosellinia anti-rot virus (Reoviridae), while S10 or S11 exhibited they were no significant sequence similarities to the known sequences published.Key words: Cryphonectria parasitica, CpMYRV1, molecular characterization.
Genetic Transformation of Transcription Factor (35S-oshox4) Gene into Rice Genome and Transformant Analysis of hpt Gene by PCR and Hygromycin Resistance Test ENUNG SRI MULYANINGSIH; REGY HERMAWAN; INEZ HORTENZE SLAMET LOEDIN
Biodiversitas Journal of Biological Diversity Vol. 10 No. 2 (2009)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d100202

Abstract

Global warming, climate change and crop extensification in marginal dryland areas are related to long dry season and waterdeficit. The water availability is an important factor in improving plant production. Application of drought tolerant rice cultivars is one of several options that might be used. Genetic engineering at the level of transcription factors is particularly promising strategy to develop drought tolerant rice varieties. Transcription factors regulate a wide range of target genes in which of them contribute to stress tolerance. HD Zip genes are transcription factor that potential in the adaptation of plants to some environment stresses including water deficit. HD-ZIP oshox4 (oryza sativa homeobox) gene controlled by 35S promotor is inserted into pCAMBIA 1300 vector with hpt (hygromycin) gene as a selectable marker. The aim of this research is to obtain transgenic rice plant from transformation with 35S-oshox4 plasmid, segregation analysis of marker gene (hpt) by PCR method at T0 and T1 generation, and hygromycin resistance analysis of seeds. Recombinant plasmid was transformed into rice genome of IRAT 112 and rojolele cultivars using Agrobacterium tumefaciens. The results showed thattransformation efficiency of IRAT 112 was 5.7-13.6% and 26-66,7% for rojolele. While regeneration efficiency for IRAT 112 is 4.7-43.7% and 23-44.1% for rojolele. The result of hygromycin resistance test at T1 seeds were obtained 14 lines cv. rojolele segregation Mendelian for hpt gene. The PCR analysis using specific primers for hpt gene at the parent (T0) from 14 lines showed that 7 lines contain the gene. At the second generation (T1), PCR analysis using hpt primers showed that 3 from 4 lines were followed Mendelian segregation pattern by the presence of specific band.Key words: HD-Zip oshox (oryza sativa homeobox), 35S-oshox4, hpt, Agrobacterium tumefaciens, PCR.
PCR-Based EST Mapping in Wheat (Triticum aestivum L.) TATIK CHIKMAWATI; MIFTAHUDIN MIFTAHUDIN; J. PERRY GUSTAFSON
Biodiversitas Journal of Biological Diversity Vol. 10 No. 2 (2009)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d100201

Abstract

Mapping expressed sequence tags (ESTs) to hexaploid wheat is aimed to reveal the structure and function of the hexaploid wheat genome. Sixty eight ESTs representing 26 genes were mapped into all seven homologous chromosome groups of wheat (Triticum aestivum L) using a polymerase chain reaction technique. The majority of the ESTs were mapped to homologous chromosome group 2, and the least were mapped to homologous chromosome group 6. Comparative analysisbetween the EST map from this study and the EST map based on RFLPs showed 14 genes that have been mapped by both approaches were mapped to the same arm of the same homologous chromosome, which indicated that using PCRbasedESTs was a reliable approach in mapping ESTs in hexaploid wheat.Key words: wheat, EST, PCR, homologous.
Population Status of Cacatua sulphurea parvula and Trichoglossus euteles in Alor, East Nusa Tenggara WAHYU WIDODO
Biodiversitas Journal of Biological Diversity Vol. 10 No. 2 (2009)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d100205

Abstract

The aim of this survey was to know the abundance status of the Yellow-crested Small Cockatoo (Cacatua sulphurea parvula) and Yellow-headed Lorikeet (Trichoglossus euteles) in Alor, East Nusa Tenggara Province. There were four observation sites in the survey, namely: (i) Air Mancur preparatory Village (in and around of Tuti Adigae Natural Tourism Park, East Alor Sub-district), (ii) Tanglapui Village (Kampong Irawuri) (East Alor Sub-district), (iii) Probur Village (Kampong Wormanem) (Southwest Alor Sub-district), and (iv) Bota Village (Northwest Alor Sub-district). The survey was used for the bird encounter rates per unit time method. The bird population was estimated by abundance score value. Fifty species of birds or 64.9% of 77 species of birds occurring in Alor were recorded during field work. The yellow-headed lorikeet observed was more abundant with five abundance score and for the numbers per 10 hours observation were 58.06. There was no yellow-crested small cockatoo observed during the survey. However, the yellow-crested small cockatoo was presumed to be present in the fields by the direct observation of old nest site of Canarium trees.Key words: parrot, population, Cacatua sulphurea parvula, Trichoglossus euteles, Alor
Abundance and Diversity of Mould Inhabiting Muara Layang Estuary Sediment, Bangka Belitung Islands SEKAR LARASHATI; MUHAMMAD ILYAS
Biodiversitas Journal of Biological Diversity Vol. 10 No. 2 (2009)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d100204

Abstract

Estuary basin is a fertile area which has important role for sustaining many organisms from estuary and sea. Mould andother saprobic microorganisms have important role to decomposing organic material in estuary water. A study on diversity and abundance of mould inhabiting Muara Layang estuary sediment, Bangka Belitung Islands has not been conducted before. The objective of this study is to investigate the abundance and diversity of mould inhabiting Muara Layang estuary sediment, Bangka Belitung Islands. The mould isolation was based on sample dilution method with Rose BengalChloramphenicol Agar mould isolation media. The abundance of mould was counted by measuring the average Colony Forming Unit (CFU)/ml of all mould colonies which grown on isolation media by Total Plate Count (TPC) method. The diversity of isolated mould was identified based on phenotypic characters by observing both of macroscopic and microscopic mould morphology. The result showed that the growth average of mould colonies is about (5-27.5) x102 CFU/mL. The result of mould identification showed that eight mould genera, Aspergillus (6 species), Chaetomium, Eupenicillium (3 species), Gliocladium, Paecilomyces, Penicillium (3 species), Scopulariopsis, Trichoderma (3 species), one group identified in class level (Coelomycetes), and three groups of unidentified sterile mould isolates were isolated.Key words: estuary, mould, sediment, abundance, diversity.
Characterizing Social Interactions and Grouping Patterns of Sumatran Orangutans (Pongo abelii) in the Gunung Leuser National Park, Sumatra JITO SUGARDJITO
Biodiversitas Journal of Biological Diversity Vol. 10 No. 2 (2009)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d100207

Abstract

The character of social interactions and grouping patterns of Sumatran orangutans (Pongo abelii) have been studied in Ketambe research station of the Gunung Leuser National Park, Sumatra. A total number of 141 groupings and 47 incidences of interactive behavior were observed during the course of study. The character of groups (feeding group or travel-band) and the type of food trees (fig tree or fruit tree) appear to influence the interactive behavior of individual orangutans. Intolerance behavior has been characterized by feeding group in large fig trees, whereas tolerance and sexual behavior were shown mostly in travel-band.Key words: feeding group, travel-band, social interaction, Sumatran orangutan, Pongo abelii.
The Occurrence of Hyperhydricity on Several Carnations (Dianthus carryophyllus L.) Cultivars during Low Temperature Storage KURNIAWAN BUDIARTO
Biodiversitas Journal of Biological Diversity Vol. 10 No. 2 (2009)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d100209

Abstract

The incident of hyperhydricity was a common problem in propagated carnation (Dianthus caryophyllus L.) during in vitroculture. Due to its possible relations with the decrease in phenotypic performance of plantlets, the observation on theoccurrence of hyperhydricity was conducted on several in vitro conserved carnation cultivars. The research was conductedfrom July 2007 to August 2008 at The Indonesian Ornamental Crops Research Institute, Cianjur, West Java. A completefactorial experiment with 24 replications was designed to accomplish the combination of two factors. The first factor was six commercial carnation cultivars, namely light pink candy, malaga, opera, white candy, liberty and pink maladi, while theseconds dealt with type of conservation media, i.e. ½MS+DMSO 3%, ½MS+DMSO 3%+3% sucrose and control (½MS+3% sucrose). The results showed that the percentage of hyperhydric plantlet and plantlet viability after in vitro conservation were varied among carnation cultivars. Single treatment of sucrose had the least capacity in inducing plantlet resistance to low temperature conditions during in vitro conservation. Supplemental DMSO postponed the occurrence of hyperhydricityand with the existence of sucrose, higher plantlet viability were achieved.Key words: carnation (Dianthus caryophyllus L.), cultivar, hyperhydricity, plantlet viability, in vitro conservation.
Cultivation Practices and Knowledge of Local Rice Varieties among Benuaq Farmers in Muara Lawa District West Kutai, East Kalimantan-Indonesia MEDI HENDRA; EDI GUHARDJA; DEDE SETIADI; EKO BAROTO WALUJO; YOHANES PURWANTO
Biodiversitas Journal of Biological Diversity Vol. 10 No. 2 (2009)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d100208

Abstract

This research aim to study how Benuaq society around Muara Lawa District, manage agriculture system and internal knowledge about rice varieties. This research use ethnobotanical approach: collecting ethnobotanical data of rice varieties and traditional system of swidden cultivation. Data was obtained by using direct participatory technique by interviewing the people (key informants) about their practice and perception. The interviews were unstructured open-ended discussion onknowledge and farming activities including about diversity in rice varieties. Subsequently, quantitative data from questionnaires was combined with depth-interview data from key informants. The Benuaq indigenous systems, practices, and cultivation preferences held by the Benuaq are guarded toward maintaining their rice diversity. The Benuaq systems of upland rice cultivation, site selection for umaq establishment, plant species for fertility indicator, and cultivation of wide ranges of upland rice varieties indicate their sophisticate knowledge in agriculture. Unfortunately there has been degradation in the indigenous knowledge among the young Benuaqs.Key words: Benuaq, ethnobotanical, indigenous, upland rice.
Reef Fish Status in Northern Acehnese Reef Based on Management Type EDI RUDI; SAYYID AFDHAL ELRAHIMI; TASRIF KARTAWIJAYA; YUDI HERDIANA; FAKHRIZAL SETIAWAN; SHINTA T. PARDEDE; STUART J. CAMPBELL; JERKER TAMELANDER
Biodiversitas Journal of Biological Diversity Vol. 10 No. 2 (2009)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d100206

Abstract

The objectives of this study were to provide reliable data and information on reef fish in the northern Aceh region of Wehand Aceh islands based on management types. This research use underwater visual census techniques at 29 sites aroundWeh Island and Aceh Islands. Reef fish abundance in area that were protected under the management of Panglima Laot ofSabang Weh Island (32505 no.ha-1) and biomass (748 kg.ha-1) were also significantly higher than in Aceh Islands which is open access areas (abundance=9539 no.ha-1; biomass=396 kg.ha-1). Fish species from the family Pomacentridae (damsel fish) were highest in abundance and biomass at northern Acehnese reef. Population of carnivorous fish such as parrotfish (Family Scaridae) was low in all study sites which indicated that northern Acehnese reef is lack of reef resilience both in managed and open access area. Higher numbers and abundance of reef fish in marine managed areas, such as Panglima Laot in Sabang, could be considered for further conservation purpose of marine resources.Key words: Coral reef, reef fish, management type, northern Acehnese reef

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