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Biodiversitas Journal of Biological Diversity
ISSN : 1412033X     EISSN : 20854722     DOI : https://doi.org/10.13057/biodiv/d230231
The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was accredited by DGHE, Ministry of Education, R.I., since 2014 was indexed by Scopus, where DOAJ and Google Scholar was indexed first.
Articles 64 Documents
Search results for , issue "vol. 22 no. 6 (2021)" : 64 Documents clear
Potential of phosphate solubilizing fungi isolated from peat soils as inoculant biofertilizer Deni Elfiati; Delvian DELVIAN; Hamidah Hanum; Arida Susilowati; Henti Hendalastuti Rachmat
Biodiversitas Journal of Biological Diversity Vol. 22 No. 6 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220605

Abstract

Abstract. Elfiati D, Delvian, Hanum H, Susilowati A, Rachmat HH. 2021. Potential of phosphate solubilizing fungi isolated from peat soils as inoculant biofertilizer. Biodiversitas 22: 3042-3048. Phosphate-solubilizing fungi are the microbes that have the ability to dissolve insoluble phosphate and made it available for plants. Therefore, the purpose of this study was to obtain the phosphate-solubilizing fungi from peat soils. Peat soil samples were taken in a composite at a depth 0-20 cm from the peat ecosystem in Nagasaribu Village, Lintong Nihuta Sub-district, Humbang Hasundutan District, North Sumatra, Indonesia. Soil samples were isolated to obtain phosphate-solubilizing fungi using the Pikovskaya selective medium. The obtained isolates were tested for their ability to dissolve phosphate qualitatively by calculating the dissolution index values and quantitatively by calculating the available phosphorus on Pikovskaya medium by using four phosphate sources, namely Ca3(PO4)2, AlPO4, FePO4, and phosphate rock. Total of 12 isolates of phosphate-solubilizing fungi was obtained during the present study. Based on the results of qualitative and quantitative testing, all 12 isolates have the ability to release phosphate from the four tasted phosphate sources. The qualitative test obtains dissolution index values that vary from 2.55 to 4.25, while quantitatively, the isolates were able to dissolve phosphate in the value range from 17.77 ppm to 69.86 ppm. The top five fungal isolates with highest phosphate-solubilizing potential were FG5, FG8, FG9, FG11, and FG12. Based on molecular identification, these five isolates were identified as Aspergillus niger.
The genetic diversity of ayam ketawa (Gallus gallus domesticus, Linneaus, 1758) in Bangkalan District, Madura Island, Indonesia ABINAWANTO Abinawanto; TRI ZULISTIANA; RETNO LESTARI; ASTARI DWIRANTI; ANOM BOWOLAKSONO
Biodiversitas Journal of Biological Diversity Vol. 22 No. 6 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220617

Abstract

Abstract. Abinawanto, Zulistiana T, Lestari R, Dwiranti A, Bowolaksono A. 2021. The genetic diversity of ayam ketawa (Gallus gallus domesticus, Linneaus, 1758) in Bangkalan District, Madura Island, Indonesia. Biodiversitas 22: 3145-3155. Ayam Ketawa, a variation of Gallus gallus domesticus Gallus gallus domesticus, comes from  Sidendreng-Rappang (Sidrap) District, South Sulawesi, Indonesia. Apart from Sidrap, ayam ketawa farms are also found in Bangkalan District, on the island of Madura, Indonesia. Ayam ketawa from Bangkalan District often wins national level contests. Therefore, the research was carried out with the aim of knowing the genetic diversity of ayam ketawa in Kamal Subdistrict, Socah Subdistrict, and Burneh Subdistrict, based on bioacoustic, morphometric, and barcoding DNA analysis. The studied ayam ketawa consists of dangdut and slow types. The longest duration (6.00 ± 3.0) seconds was shown by ayam ketawa from Kamal Subdistrict, while the highest number of syllables (7.20 ± 5.80) came from Socah Subdistrict. The results of the Mann-Whitney test showed that eight morphological characters were significantly different (P <0.05) between ayam ketawa population in Kamal and Burneh subdistricts, namely body weight, femur length, shank length, shank circumference, wing length, comb height, third finger length, and breastbone length. Four morphological characters differed significantly (P <0.05) between ayam ketawa population in Kamal and Socah subdistricts, namely shank length, wing length, third finger length, and sternum length. Three morphological characters were significantly different (P <0.05) between the Burneh and Socah subdistricts population, namely body weight, femur length, and shank circumference. The analysis results of DNA Barcoding gene of the Cytochrome Oxidase subunit 1 (CO1) showed a genetic distance ranging from 0.025 to 1.872.
Genetic diversity of Pleurotus ostreatus (Jacq.) P. Kumm. strains in Java based on random amplified polymorphic DNA markers NURAENI EKOWATI; ARIS MUMPUNI; JUNI SAFITRI MULJOWATI; NUNIEK INA RATNANINGTYAS; ARDHINI RIN MAHARNING
Biodiversitas Journal of Biological Diversity Vol. 22 No. 6 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220658

Abstract

Abstract. Ekowati N, Mumpuni A, Muljowati JS, Ratnaningtyas NI, Maharning AR. 2021. Genetic diversity of Pleurotus ostreatus (Jacq.) P. Kumm. strains in Java based on Random Amplified Polymorphic DNA markers.  Biodiversitas 22: 3488-3493. Genetic variation in a fungal population can occur due to mutation and recombination, resulting in changes in the nucleotides that encode specific DNA sequences. Strains with a high genetic distance and good production capabilities can be used to develop genetic breeding. This study aimed to investigate genetic relationship among Pleurotus ostreatus strains cultivated in Java (Bogor, Cianjur, Tasikmalaya, Purwokerto, Yogyakarta, Tawangmangu, Malang, and Madiun) based on random amplified polymorphic DNA (RAPD) markers.  The research method consisted of DNA isolation and DNA amplification using six primers, i.e. OPA2, OPA3, OPA4, OPA7, OPA9, and OPA10. DNA band data were analyzed using NTSYSpc21 software to determine the level of genetic similarity, based on the Unweighted Pair Group Method with Arithmetic Average Algorithm (UPGMA). In all, 101 amplified DNA bands were obtained, with sizes ranging from 136 to 2320 bp and 96.0% of the bands were polymorphic. Based on cluster analysis, it shows that three clusters were formed. There were genetic variations and relationships among eight P. ostreatus strains in Java with a genetic similarity varying from 37-98%.
Soil macrofauna diversity in andisol after eight years of Mount Sinabung eruption in Sumatra, Indonesia mariani sembiring; HIDAYATULAH MUNAWAROH; MUKHLIS MUKHLIS; BENNY HIDAYAT; TENGKU SABRINA
Biodiversitas Journal of Biological Diversity Vol. 22 No. 6 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220603

Abstract

Abstract. Sembiring M, Munawaroh H, Mukhlis, Hidayat B, Sabrina T. 2021. Soil macrofauna diversity in andisol after eight years of Mount Sinabung eruption in Sumatra, Indonesia. Biodiversitas 22: 3024-3030. The eruption of Mount Sinabung resulted in volcanic ash covering the soil of various thicknesses. That will affect the population and diversity of macrofauna in it. This research aimed to determine the Andisol soil macrofauna in Karo District with various thicknesses of volcanic ash covering from Mount Sinabung. This research was conducted in May 2019. Plots were placed in four locations, Location I: processed land (0 cm), Location II: Land covered by thin ash (?2 cm), Location III: Land covered by medium ash (2-5 cm), Location IV: Land covered by thick ash (?5 cm). Sampling was conducted by using the Pitfall trap, Monolith squared, and Hand sorting methods. The research results indicated that the thicker the volcanic ash covering the soil surface, it would reduce soil moisture, soil water content, organic C, and soil pH, but on the other hand, increase the soil temperature. A total of 20 species were able to live on the Andisols affected by the eruption of Mount Sinabung.
Ethnobotanical study based on the five dimensions of basic life needs in Tidung Tribe of North Kalimantan, Indonesia Alfi Suciyati; I Gusti Putu Suryadarma; Paidi Paidi; Fadhlan Muchlas Abrori Abrori
Biodiversitas Journal of Biological Diversity Vol. 22 No. 6 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220623

Abstract

Abstract. Suciyati A, Suryadarma IGP, Paidi Abrori FM. 2021. Ethnobotanical study based on the five dimensions of basic life needs in Tidung Tribe of North Kalimantan, Indonesia. Biodiversitas 22: 3199-3208. The Tidung tribe is one of the original inhabitants of North Kalimantan, Indonesia. Research results related to the Tidung tribe, especially those related to ethnobotany, are still rare. This research aimed to make an inventory of plant species used by Tidung Tribe based on the five dimensions of basic life needs of Tidung Tribe. The five dimensions of basic life needs of Tidung Tribe is that plants are useful as food (ngakan), shelter (baloy), health/medicine (sihat), traditional ceremony (adat), and clothing (memana). Informants were obtained by conducting snowball sampling consisting of 22 family heads and as many as 65 people. There were three instruments in the data collection: questionnaires of list of plant species, quantitative plant assessment instruments, and semi-structured interviews. According to the inventory result, Tidung Tribe utilize 60 plant species in their daily life. Most of these plants are used as food (ngakan) and traditional medicine (sihat). The Availability Index and Frequency Use Index data showed that Arecaceae, Pandanaceae, Solanaceae, Zingiberaceae, and Poaceae families were the most widely available plants in the research location and most often used by the community.
Comparison of growth pattern, condition factor, gonadosomatic index of Manggabai (Glosogobius giuris) and Payangka (Ophieleotris aporos) in Limboto Lake, Gorontalo, Indonesia Hasim Hasim; Jali Tuheteru; Dewi Nuryanti Fazrin
Biodiversitas Journal of Biological Diversity Vol. 22 No. 6 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220646

Abstract

Abstract. Hasim, Tuheteru J, Fazrin DN. 2021. Comparison of growth pattern, condition factor, gonadosomatic index of Manggabai (Glosogobius giuris) and Payangka (Ophieleotris aporos) in Limboto Lake, Gorontalo, Indonesia. Biodiversitas 22: 3388-3393. Manggabai and Payangka are endangered native fishes in Limboto Lake due to continuous degradation in the lake. In addition, silting lake along with its area that continues to narrow can threaten the life of both fishes although the government has stipulated restoration of Limboto Lake as the ecosystem of the fishes. However, the current information on the fishes remains considerably limited in terms of making it as a conservation strategy. This study aims to analyze the relationship between the total length and weight of Payangka and Manggabai. Furthermore, it analyzes the condition factors and the gonadosomatic index of the two fishes. The research method integrates survey and laboratory techniques. The fishes are collected by using trap made of bamboo and motorcycle tires that are installed for three days and the catches are 60 fishes including 30 Manggabai and 30 Payangka. Then the total length and weight of the fish and their gonad weight were measured. Data analysis used mathematical formulation of the relationship between total weight and length, condition factor, and gonadosomatic index. The result of the analysis shows that the growth pattern of the Manggabai was negative allometric whereas the Payangka was positive allometric. The condition factor for both types is generally less than 1.7. The gonadosomatic index of Manggabai ranges from 4.10-20.00% and Payangka ranges from 0.05-1.98%. These conditions indicate that differences in growth pattern, condition factor, and gonadosomatic index are different forms of response to environmental conditions and in the spawning season of both types of fish.
Phenotypic characters and identification CYPs (Cyclophilin) gene in Cucumis melo L. cv. Gama Melon Parfum WIKO ARIF WIBOWO; SIGIT DWI MARYANTO; BUDI SETIADI DARYONO
Biodiversitas Journal of Biological Diversity Vol. 22 No. 6 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220601

Abstract

Abstract. Wibowo WA, Maryanto SD, Daryono BS. 2021. Phenotypic characters and identification CYPs (Cyclophilin) gene in Cucumis melo L. cv. Gama Melon Parfum. Biodiversitas 22: 3007-3014. Cucumis melo L. cv. Gama Melon Parfum is a new cultivar with a very strong fragrance as its main character. As a new cultivar with unique characters, it is necessary to characterize phenotype and molecular related to the fragrant aroma. The research aimed to study the phenotypic of the fruit of C. melo cv. Gama Melon Parfum (F3) and to identify the CYPs gene as one of the genes that act in encoding volatile compounds. Analysis of qualitative characters was based on International Plant Genetic Resources Institute (IPGRI) and Plant Variety Protection (PVP), phenotypic characters in melons observed by abiding the Rules for Registration of Varieties from the Indonesian Minister of Agriculture Decree No. 700/Kpts/OT.320/D/12/2011, while analysis of quantitative characters was using the ANOVA methods and software PKBT-STAT-2. The methods of molecular characterization included RNA isolation, cDNA synthesis used Reverse Transcriptase-PCR, amplification of DNA target used PCR, visualization of DNA target used electrophoresis, and DNA sequencing. Analysis of the in silico approach was carried out on the CuGenDB Melon database. Analysis of protein sequences and classification was obtained from InterPro. Phylogeny analysis using MEGA-X Software. The results were 18 qualitative characters and 11 quantitative characters were stable and uniform, whereas the molecular characterization of the genes was predicted Cyclophilin with peptidylprolyl isomerase (PPIase) activity and located in chromosome 1 (17059021-17058899).
Development of phenotypic markers and contrast genotype candidates of target minerals related to cassava Sylvia Nosya Pratama; Sudarsono SUDARSONO; Sintho Wahyuning Ardie; NURUL KHUMAIDA; Dewi Sukma
Biodiversitas Journal of Biological Diversity Vol. 22 No. 6 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220606

Abstract

Abstract. Pratama SN, Sudarsono, Ardie W, Khumaida N, Sukma D. 2021. Development of phenotypic markers and contrast genotype candidates of target minerals related to cassava. Biodiversitas 22: 3049- 3056. Cassava leaves have various macro and micro-nutrients with varying levels, such as magnesium, iron, and zinc. The differences in genotypes of cassava may result in different mineral content in cassava leaves. This study aims to determine the estimator phenotypic markers and contrast genotype candidate selection related to target minerals. This study used a one-factor treatment composed of 12 cassava genotypes and arranged in a randomized block design (RBD) which each treatment was repeated 3 times. The observation was conducted on the leaves' qualitative and quantitative characters such as plant growth, leaf morphology, and leaf mineral content characters. The observation was performed at 3 and 6 months after planting. The results showed that the quantitative and qualitative characters that could be used as estimator for mineral content in cassava leaves are the Soil Plant Analysis Development (SPAD)- Chlorophyll value and leaf color. Genotypes G6-1-15-4-3, G6-2-15-1-1, G6-2-15-3-3 (lowest) and Malang (G4D0), G4D1-222, G3D2-413 (highest) could be categorized as contrast genotypes based on SPAD-value and mineral content. The strong positive correlation between Mg and Fe content with SPAD suggested that SPAD is a possible marker for high Mg and Fe content in leaves. Adversely, a weak relationship between SPAD and Zn content in leaves showed that SPAD is a weak marker for Zn content in leaves. Further research related to molecular markers development can be carried out based on these results.
Short Communication: Ethnobotanical and ecological studies of medicinal plants in a mangrove forest in Mempawah District, West Kalimantan, Indonesia Yanieta Arbiastutie; Farah Diba; Masriani Masriani
Biodiversitas Journal of Biological Diversity Vol. 22 No. 6 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220619

Abstract

Abstract. Arbiastutie Y, Diba F, Masriani. 2021. Short Communication: Ethnobotanical and ecological studies of medicinal plants in a mangrove forest in Mempawah District, West Kalimantan, Indonesia. Biodiversitas 22: 3164-3170. The mangrove forest Polaria Tanjung Pagar in Mendalok Village, Mempawah District, West Kalimantan, Indonesia, is a mangrove forest maintained by the local community. Currently, this mangrove forest has become one of the tourist attractions, not only visited by local and national, but also foreign tourists. This study aims to identify plants in Polaria Tanjung Pagar mangrove forest Kalimantan with the potential for medicinal uses. The research used a survey method in the mangrove forest by conducting an inventory of the medicinal plants found in the mangrove forest. Observations were made using a transect line with the total area understudy of 0.012 ha and sampling intensity of 0.4%. The investigation was based on cross-checking the traditional use of medicinal plants by the surrounding communities and the scientific literature. The results showed 13 types of medicinal plants (including tree and understorey plants) from 6 different families, i.e., Acrostichum aureum, Aegiceras corniculatum, Avicennia marina, Avicennia alba, Bruguiera parviflora, Bruguiera gymnorrhiza, Bruguiera cylindrica, Ceriops decandra, Rhizophora apiculata, Rhizophora stylosa, Rhizophora mucronata, Xylocarpus moluccensis, and Sonneratia caseolaris. The community structure of the mangrove forest had moderate diversity for tree species and low diversity for understorey plant species, with an H' valued at 2.39 and 0.25, respectively.
Ethnobotanical survey of medicinal plants used by the Mamanwa tribe of Surigao del Norte and Agusan del Norte, Mindanao, Philippines Olga Nuneza; BIVERLY RODRIGUEZ; Juliet Grace Nasiad
Biodiversitas Journal of Biological Diversity Vol. 22 No. 6 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220634

Abstract

Abstract. Nuneza OM, Rodriguez BC, Nasiad JGM. 2021. Ethnobotanical survey of medicinal plants used by the Mamanwa tribe of Surigao del Norte and Agusan del Norte, Mindanao, Philippines. Biodiversitas 22: 3284-3296. Traditional knowledge on medicinal plants plays an important role in public healthcare and development of drugs. In the Philippines, studies on ethnomedicinal plants have increased throughout the years. However, documentation on the ethnobotanical knowledge in Mindanao is few and mostly focused on other well-known tribes. This ethnobotanical study was carried out to document medicinal plants used by the Mamanwas tribe in ten Indigenous Cultural Communities (ICCs) of Surigao del Norte and Agusan del Norte, Philippines. Ethnomedicinal information on the plant parts used, different modes of preparation, and mode of utilization was gathered from 143 local informants through informal interviews and semi-structured questionnaires. Seventy-eight plant species under 70 genera and 42 families were documented to treat various ailments in their communities. Family Asteraceae held the dominance with ten species that were reported to have medicinal uses. Out of the 78 species, trees constituted the largest proportion with 32 plant species that cover 41%. In terms of plant parts used, leaves are frequently used constituting 46%. As to mode of preparation, decoction (40%) ranked the highest followed by poultice (18%) and heating over fire (14%). Documentation of the valuable ethnomedicinal knowledge will help preserve the vanishing tradition of cultural communities in terms of medicinal plant utilization.

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