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INDONESIA
LenteraBio
Published by Universitas Negeri Surabaya
ISSN : 22523979     EISSN : 26857871     DOI : -
Core Subject : Education, Agriculture,
Agriculture, Biological Sciences & Forestry Education
Arjuna Subject : -
Articles 281 Documents
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PENGARUH KROMIUM HEKSAVALEN (VI) TERHADAP TINGKAT KELANGSUNGAN HIDUP IKAN NILA (OREOCHROMIS NILOTICUS) AYU MARETHA OKA WIRES, EDELYNNA A.M.O.
LenteraBio: Berkala Ilmiah Biologi Vol 1, No 2 (2012)
Publisher : Universitas Negeri Surabaya

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Abstract

Chromium is a heavy metals, pollutants, and micronutrients that are important to the body. Type of chromium that has toxic effects to the organism is hexavalent chromium. This study aims to determine the effect of hexavalent chromium on the survival rate of tilapia (Oreochromis niloticus). Experimental studies conducted in the laboratory, using a Completely Randomized Design (CRD) with one treatment factor, namely the concentration factor. Concentrations of hexavalent chromium used is 0 ppm (control), 57,69 ppm, 59,94 ppm and 83,20 ppm. The results showed that hexavalent chromium significantly affect the survival rate of tilapia. The survival rate of tilapia in a row at a concentration of 57,69 ppm; 59,94 ppm, and 83,20 ppm for 54,78; 43,08, and 0,33. The conclusions of this study is that hexavalent chromium concentration of 57,69 ppm; 59,94 ppm, and 83,20 have a significant influence on the survival rate of tilapia
PENGARUH PEMBERIAN AIR REBUSAN AKAR DELIMA (PUNICA GRANATUM L.) TERHADAP MORTALITAS ASCARIS SUUM GOESZE. SECARA IN VITRO SANDIKA, BAYU
LenteraBio: Berkala Ilmiah Biologi Vol 1, No 2 (2012)
Publisher : Universitas Negeri Surabaya

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Abstract

Pomegranate is one of plants that has a potential to treat askariasis because it contains high alkaloids and tannins, especially in the bark and roots. This study aims to prove the anthelmintic power of pomegranate root decoction water based on the rate of death or the mortality of A. suum Goesze. The research was carried out by soaking worms in the decoction water of pomegranate root which consists of four different concentrations, namely 0%, 21.56%, 43.12% and 64.68%. The research was conducted in three replications using a completely randomized design (CRD), then the data that was obtained is transformed using arcsin and analyzed by one-way ANAVA. From the research result, it can be concluded that the decoction water of pomegranate root affects to the mortality of A. suum Goesze. significantly after 48 hours exposure at a concentration of 64.68% and the characteristic of a dead worm is having a soft body and a transparent cuticle.
KEANEKARAGAMAN BIVALVIA DAN PERANANNYA SEBAGAI BIOINDIKATOR LOGAM BERAT KROMIUM (CR) DI PERAIRAN KENJERAN, KECAMATAN BULAK KOTA SURABAYA AMANDA PUTRI, RESTU Amanda
LenteraBio: Berkala Ilmiah Biologi Vol 1, No 2 (2012)
Publisher : Universitas Negeri Surabaya

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Abstract

The Kenjeran Water is one of the marine tourism in Surabaya. It is located in the fishing village of Tambak Deres. Diversity in the Kenjeran Water is very diverse, one of it is the types of bivalves. As a place for recreation, Kenjeran Water are susceptible to contamination due to the human activity comes from the domestic waste. Consequently, many heavy metals have been entering the water. The existence of marine organisms such as bivalves can be used as a bioindicator of the water quality. This study was a descriptive study, using survey and observation. Implementation began in November-December 2011 with the method of transect for each station, per 100 metre area of Kenjeran Water Surabaya, which is representative to serve as a habitat for bivalves and to measure the water quality. The result of this study indicated that The Kenjeran Water Surabaya have 6 species, among of them are Perna viridis, Trisidos tortuosa, Mactra chinensis, Mercenaria mercenaria, Anadara granosa and Anadara floridana. The highest indices of diversity and abundance were 1.978 and 37.702. The average levels of chromium in the water, sediment, and the meat was 0.052 mg/l; 22.71 mg/l; and 49.25 mg/l. Based on analysis of chromium contamination, the Kenjeran Coastal?s water were polluted with chromium on medium level. Its Bivalves are unfit for consumption. Based on this, the bivalves are a bioindicator for Cr polluted waters.
PENGARUH PEMBERIAN FILTRAT DAUN ALANG-ALANG (IMPERATA CYLINDRICA L.) TERHADAP PERTUMBUHAN MISELIUM JAMUR TRICHODERMA SP. YANG HIDUP PADA MEDIA TANAM JAMUR TIRAM PUTIH (PLEUROTUS OSTREATUS) KURNIA WATI, DIAN Kurnia
LenteraBio: Berkala Ilmiah Biologi Vol 1, No 2 (2012)
Publisher : Universitas Negeri Surabaya

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Abstract

The genus Trichoderma is cosmopolitan in soil, on decaying wood and vegetable matter. Infections in mushroom cultivation due to member of genus Trichoderma have come to be know as the ?Green Mould? dieases. Among the fungal pathogens, Trichoderma viridae are the most common dieases that can causes loss in Oyster mushroom (Pleurotus ostreatus) cultivation. If Trichoderma have been found on cultivated Oyster mushroom (Pleurotus ostreatus), the farmers  should throw it away the baglog, to prevant another baglog contaminated. This research aimed to investigate the effect of adding alang-alang (Imperata cylindrica L.) leves filtrate on the growth of Trichoderma sp. cultured on Pleurotus ostreatus media. This research use the Complete Randome Disign (CRD) with 5 treatment consentration. The concentration was:  10%,20%, 30%, 40% dan 0% (controlled). Each treatment replicated into five times. The result, which includes the width surface of mycellium colony of Trichoderma sp. and the width surface of mycellium colony of oyster mushroom (Pleurotus ostreatus), is descriptively analyzed. Based on the average width of both surface mycellium colony, it is known that there is significant different growth. The lowest width of Trichoderma sp. growth which found on the 40% concentration is 7,925 cm2. The highest width of oyster mushroom (Pleurotus ostreatus) growth which found on the 40% concentration is 2063,200 cm2.
PENGARUH PEMBERIAN FILTRAT BAWANG MERAH DENGAN BERBAGAI KONSENTRASI DAN ROOTONE-F TERHADAP PERTUMBUHAN STEK BATANG TANAMAN JAMBU BIJI (PSIDIUM GUAJAVA L) MAYASARI, EVA
LenteraBio: Berkala Ilmiah Biologi Vol 1, No 2 (2012)
Publisher : Universitas Negeri Surabaya

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Abstract

Psidium guajava L. has a grade potensial in domestic market. The aim of research was to find out the different of concentrations of shallots extract and Rootone-F on vegetative growth of Psidium guajava L. by stem cutting. Complete Randome Design was used as the research methode in with 4 different concentrstions and 6 replicates. The result revealed that the concentrations of shallot extract affected on the vegetative growth. Beside that the result showed that concentrations was 18%-20% showed the best result for all growth parameters observed i.e. number of shoots, number of leaves, leaves wide, and number of roots.
RESISTENSI PARAMAECIUM CAUDATUM TERHADAP LOGAM TEMBAGA (CU) DENGAN PEMBERIAN PAKAN PSEUDOMONAS FLUORESCENS PADA MEDIA JERAMI AJENG DIAH VITALOCHA, GALUH A.D.
LenteraBio: Berkala Ilmiah Biologi Vol 1, No 2 (2012)
Publisher : Universitas Negeri Surabaya

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Abstract

Paramaecium caudatum to high copper metal in the water, because it can be used as an indicator of environtmental pollution. This study aims to determine the level of resistance to copper metal Paramecium caudatum with Pseudomonas fluorescens feeding hay on the media. So it can be used as a landing bioindicator determination of pollution. This study is a study with a control experiment, four treatmants and six repetitions. The study design used was Randomized Complete. Design concentration of copper used in this study was 1 ppm, 2 ppm, and 3 ppm. Data collection is done by calculating the population of Paramaecium caudatum every day for 10 days after breeding in a solution of copper metal. Data analysis using quantitative descriptive statistics where the data is establised in kurva growth. The result showed Paramaecium caudatum resistance to metalic copper in this study were at a concentration of 2 ppm.
PENGARUH KOMBINASI KOMPOS TRICHODERMA DAN MIKORIZA VESIKULAR ARBUSKULAR (MVA) TERHADAP PERTUMBUHAN TANAMAN KEDELAI (GLYCINE MAX (L.) MERILL) PADA MEDIA TANAM TANAH KAPUR MEGA CHARISMA, ACIVRIDA Mega
LenteraBio: Berkala Ilmiah Biologi Vol 1, No 3 (2012)
Publisher : Universitas Negeri Surabaya

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Abstract

Soybean productivity should be increased because of the increasingly strong demand. One method is the large of plant area. Therefore calcareous soil can be used as an alternative grown medium, but the contrains in the calcareous soil should be overcome. On of alternative effort to overcome the deficiencies of nutrients in the calcareous soil is the addition of Trichoderma compost and vesicular arbuscular mycorrhizal (VAM). The purpose of this study was to determine the influence of the compost on the soybean grown on the calcareous soil. Experimental study was conducted using Randomized Completely Block Design (RCBD) by a factor of Trichoderma compost treatment (0 g, 15 g, 30 g and 45 g) with six replications. Measured parameters were the percentage of mycorrhizal infection, the percentage of active root nodules, plant height, root length, biomass of soybean. Data were analyzed using one-way ANAVA and continued to by the LSD if there were a significant different. The results showed that of compost consist Trichoderma and VAM affected significantly on the percentage of VAM infection, the percentage of active root nodules, plant height, root length, and biomass plants. The most optimal concentration of Trichoderma compost from all five parameters is 45 grams.
PENGARUH PEMBERIAN ASAM LAURAT TERHADAP APOPTOSIS SEL HELA SECARA IN-VITRO PRANATA EFFENDI, DIDIT Pranata
LenteraBio: Berkala Ilmiah Biologi Vol 1, No 3 (2012)
Publisher : Universitas Negeri Surabaya

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Abstract

Cervical cancer is the most woman cancer in the world. It caused by Human Papilloma Virus (HPV) which can inactivate some genes that have crucial role on cell cycle and apoptosis. Some cancer treatment such as surgery, chemotherapy and radiotherapy are proved ineffectively. We observed potentially of lauric acid as cancer herbal therapy. Lauric acid has anti free radical activity that important for cancer treatment. This study aim is to observe about the effect of laurid acid on HeLa cell apoptosis and expression of caspase 9 by using in-vitro. The apoptosis of HeLa cell is analyzed by caspase 9 protease enzyme absences by using immunocytochemistry with primary antibody anti caspase 9 and secondary antibody anti rabbit biotin. Percent apoptosis is measured by ratio between numbers of caspase 9 expression HeLa cell and total HeLa cell number times by 100%. The number of cell is counted by microscopy observation using difference color of cell on 5 areas. The brown color show the caspase 9 expression HeLa cell and violet color show the live HeLa cell. The result show that 0,0125 µl/ml of lauric acid giving posses highest caspase 9 expression and percent apoptosis on HeLa cell in-vitro.The apoptosis of HeLa cell is increase along with increasing of lauric acid concentration.
PERTUMBUHAN MISELIUM DAN PRODUKSI TUBUH BUAH JAMUR TIRAM PUTIH (PLEUROTUS OSTREATUS) DENGAN MEMANFAATKAN KULIT ARI BIJI KEDELAI SEBAGAI CAMPURAN PADA MEDIA TANAM SUHARNOWO,
LenteraBio: Berkala Ilmiah Biologi Vol 1, No 3 (2012)
Publisher : Universitas Negeri Surabaya

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Abstract

Epidermal of soya seed is very abundant but has not maximal utilized yet. One alternative that can be done is to utilize waste as additional media on the white oyster mushroom growing media. This research aim is to compare difference between mycelium growth and fruiting body production of Pleurotus ostreatus on different additional component media, and to get optimal percent combination of growing media. This research design is Completely Randomize Design (CRD) with 5 repetitions. The treatment on this research is bran and epidermal of soya seed with percent combination respectively: 80% : 20%, 50% : 50%, 20% : 80% and 100% epidermal of soya seed. This research parameter is mycelium growth and fruiting body production of Pleurotus ostreatus. This parameter is measured once tree day for 4 observation time. The data is analyzed by one way Anova with 5% significant level. The significant data will be continued by DMRT analysis. The result show that there is significantly different of mycelium growth Pleurotus ostreatus which be planted with different additional component on growing media combination. The growth average of mycelium on different percent of media combination between bran and epidermal of soya seed respectively 80% : 20%, 50% : 50%, 20% : 80% is 2,99 cm; 3,10 cm dan 2,86 cm. On the other result show that there is not significantly different of fruiting body prodution Pleurotus ostreatus which be planted with different additional component on growing media combination. This research suggest that epidermal of soya seed has a potential to substitute for bran and also is better compare with it as Pleurotus ostreatus growing media
KEMAMPUAN TANAMAN GENJER (LIMNOCHARIS FLAVA (L.)BUCH.) MENYERAP LOGAM BERAT TIMBAL (PB) LIMBAH CAIR KERTAS PADA BIOMASSA DAN WAKTU PEMAPARAN YANG BERBEDA HARYATI, MAHARANI
LenteraBio: Berkala Ilmiah Biologi Vol 1, No 3 (2012)
Publisher : Universitas Negeri Surabaya

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Abstract

The Paper Industry produces minor product in the form of sewage especially liquid sewage in a very large volume which contains of heavy metals Pb.  Furthermore,  this sewage is usually discharged into the water  which causes pollution. An effort is required to minimize the content of Pb metal before being discharged into waters, including the use of Genjer plants as the agent of phytoremediation. This study aims to determine the ability of Genjer plants to absorb the Lead metals (Pb) from liquid paper waste. The research is accomplished by doing an experiment that uses a Randomized Block Design (RGD) with two treatment factors, the biomass plant and length of exposure time. The treatment factor of plant biomass is 0 g, 50 g, 100 g and 150 g. The factor of exposure time is for 7 days, 14 days and 21 days. The parameters which are observed include the levels of Pb in the liquid paper waste, in roots of Genjer plants and leaves of Genjer plants. The analysis of Pb levels uses ICP that is done at the Energy Studies and Engineering Laboratory, Sepuluh November Institute of Technology (ITS), Surabaya. The data are statistically analyzed using 2-way Anava and further test, Duncant Multiple Range Test (DMRT). The result shows that biomass of Genjer plants and exposure time affect to the ability of Genjer plants to absorb Pb metals in liquid paper waste paper. The most optimal decrease of Pb levels occurs in the treatment of biomass Genjer 150 grams with exposure time for 21 days which is equal to 0.4688 mg / l (80.09%). Genjer plants can absorb and accumulate Pb. The highest accumulation of Pb in the root is 1.1546 mg / l (26.1%), and in the leaves is 0.1120 mg / l (21.9%), both occur at 150 g biomass treatment and exposure time of 21 days. Thus the Genjer plant can be used as fitoremediator heavy Lead metal (Pb) in the liquid paper waste.

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