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kPERHIMPUNAN MIKROBIOLOGI INDONESIA (SeKretariat PERMI), Gedung 10.2 Indonesian Life Sciences Center (ILSC), Zona Bisnis Teknologi Puspiptek, Jalan Raya Serpong - Bogor Gunung Sindur, Jawa Barat 16340, Indonesia. Email: microbiology.indonesia@gmail.com
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Microbiology Indonesia
Published by Perhimpunan Mikrobiologi Indonesia
ISSN : 19783477     EISSN : 20878575     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology
Microbiology Indonesia provides a unique venue for publishing original researches in microbiology (espesially from Indonesian reseachers), and ensures that authors could reach the widest possible audience. Microbiology Indonesia publishes a wide range of research disciplines on bacteria, archaea, fungi, protozoa, and virus as well as biotechnology related to microbiology. Topics include (but are not limited to): -methods in microbiology, -bioprocess, -environmental microbiology, -food microbiology, -plant-microbe interaction, -animal-microbe interactions, -microbial community, -microbial genetics, -virology, -comparative and functional microbial genomics, -and gene expression in microbes.
Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi dan Bioteknologi Terapan
Articles 6 Documents
 1 
Search results for , issue "Vol. 12 No. 2 (2018): June 2018" : 6 Documents clear
Lactobacillus fermentum LLB3 improves antioxidant activity of bitter melon (Momordica charantia)
Microbiology Indonesia Vol. 12 No. 2 (2018): June 2018
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (466.353 KB) | DOI: 10.5454/mi.12.2.5

Abstract

Oxidative stress plays a major role in the pathogenesis and progression of diabetes. Functional foods with an antioxidant effect can repress oxidative stress. Momordica charantia (bitter melon) contains substances with antidiabetic properties such as charantin, vicine, and polypeptide-p, as well as other unspecific bioactive components such as antioxidants. Lactobacillus fermentum LLB3 isolated from bamboo shoot pickle  was used to ferment bitter melon juice. The aim of this study was to evaluate changes in antioxidant activity of bitter melon juice during fermentation. Study has been carried out by fermenting bitter melon juice with L. fermentum LLB3. The free radical scavenging activity of the phenolics were done using 2,2-diphenyl- 1-picrylhydrazyl (DPPH).  Antioxidant activity of bitter melon juice increased during 24 hours fermentation. In addition, the sugar content and pH  decreased compared with the baseline value. The fermentation of bitter melon juice by L. fermentum LLB3 increased its antioxidant activity. These result suggest that fermented bitter melon juice is a promising agent for diabetes management.
Purification and Characterization of Glucose Oxidase of Aspergillus niger IPBCC.08.610 ANINDA INDRIANI; LAKSMI AMBARSARI; DEWI SESWITA ZILDA
Microbiology Indonesia Vol. 12 No. 2 (2018): June 2018
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (575.093 KB) | DOI: 10.5454/mi.12.2.2

Abstract

Glucose oxidase was an enzyme which catalyzed β-D-Glucose to gluconic acid and hydrogen peroxide. Glucose oxidase from Aspergillus niger IPBCC.08.610 was isolated, purified and characterized. The enzyme was purified by ammonium sulphate precipitation and dialysis. The specific activity and yield of dialysis fraction were 19.766 U/mg and 4.744%. The optimum pH and temperature were 6 and 30oC. The stability of enzyme at optimum pH and temperature was decreasing 50% at 25 minutes. The km and vmax values for enzyme were 27 mM and 0.986 U/mg.
Influence of Temperature Variations on Growth of Nostoc (Cyanobacteria) HS-5 and HS-20 Isolated from Indonesian Hot Springs NINING BETAWATI PRIHANTINI; CAHYA GUSLYANI; RATNA YUNIATI; WELLYZAR SJAMSURIDZAL
Microbiology Indonesia Vol. 12 No. 2 (2018): June 2018
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1761.766 KB) | DOI: 10.5454/mi.12.2.1

Abstract

The research aims to know the effect of variation temperature to the growth of Nostoc HS (Hot Spring)-5 and HS-20. Strain of Nostoc HS-5 was isolated from Ciseeng hot spring which has habitat temperature range of 30-43 °C, and Nostoc HS-20 was isolated from Pancar Mountain hot spring which has temperature range of 46-69 °C. The research was done by measuring biomass weight and chlorophyll content on day-1, 2, 3, 4, 7, 10, 14, 17, and 21. The temperatures used were 20 °C, 35 °C, and 50 °C. The growth medium used was Bold Basal Medium (BBM) with pH 6.6. Each treatment was made in four replications. Non-parametric statistical analysis used were the Friedman test (a=0.05) and Spearman test (a=0.01).The result showed there were significant differences on the biomass weight of Nostoc HS-5 and HS-20 grown at temperature of 20 °C, 35 °C, and 50 °C. The average amount of biomass highest weight for Nostoc HS-5 and HS-20 occurred in both strains were grown at 35 °C.  Besides that, there was no correlation between the weight of biomass and chlorophyll content of Nostoc HS-5 and HS-20.
The Addition of Dextrose to the Preparation of Culture Media from Earthworm Juice (Lumbricus rubellus) for the Production of Saccharomyces cerevisiae FNCC 3012 Inoculum
Microbiology Indonesia Vol. 12 No. 2 (2018): June 2018
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (32.6 KB) | DOI: 10.5454/mi.12.2.3

Abstract

Saccharomyces cerevisiae is one of the commonly used yeast for bioethanol production. It can be produced by growing it in a culture medium containing nitrogen and carbon. Earthworms (Lumbricus rubellus) are high in protein and have a relatively low price that can be used as a source of nitrogen for S. cerevisiae FNCC 3012. Dextrose is used as a carbon source. The objectives of the study were to determine the comparison of the growth rate of S. cerevisiae FNCC 3012 to the earthworm juice and commercial media (malt extract, meat extract, and yeast extract). The highest yield of S. cerevisiae FNCC 3012 cells were produced on the earthworm juice medium with 1% dextrose addition. The maximum specific growth rate (μmax) and the saturation constant (Ks) about 3,4.h-1 and 3,1 g.l-1, respectively.
Cloning and expression of NS2B/NS3 protein of DENV3 Indonesia strain in Saccharomyces cerevisiae expression system for the development of Dengue vaccine ASRI SULFIANTI; SABAR PAMBUDI; ISMA NUR AZIZAH; DODDY IRAWAN SETYO UTOMO; ABINAWANTO ABINAWANTO
Microbiology Indonesia Vol. 12 No. 2 (2018): June 2018
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3936.725 KB) | DOI: 10.5454/mi.12.2.4

Abstract

NS3 protein is 618 amino acids (aa) in length containing serine protease and helicase domains required for DENV replication. Alignment of consensus amino acid sequences from all four DENV serotypes demonstrated that this protein is more conserved (78%) among the different dengue serotypes, which elicits a strong cellular immune response after viral infection in humans and animal models. Present study, a central hydrophilic region of NS3 cofactor, NS2B (NS2BH) with full length of NS3 genes DENV3 Indonesian strain were amplified from CDNA following PCR, and inserted to PYES2/CT shuttle vector. The recombinant plasmid was transformed and expressed in Saccharomyces cerevisiae expression system. As result, detection with Anti-His detector and Anti-NS3 shown NS2BH/NS3 was expressed as 83 KDA protein band. We found that addition of NS2BH on NS3 full length construction plasmid increase the yield and activity of protein expression in S. cerevisiae. In future study, our recombinant NS2B/NS3 protein can be used as recombinant protein in Dengue vaccine development.
ITA REGISTRATION FORM AND BACK COVER
Microbiology Indonesia Vol. 12 No. 2 (2018): June 2018
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (970.339 KB) | DOI: 10.5454/mi.12.2.%p

Abstract

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