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Iman Rusmana
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kPERHIMPUNAN MIKROBIOLOGI INDONESIA (SeKretariat PERMI), Gedung 10.2 Indonesian Life Sciences Center (ILSC), Zona Bisnis Teknologi Puspiptek, Jalan Raya Serpong - Bogor Gunung Sindur, Jawa Barat 16340, Indonesia. Email: microbiology.indonesia@gmail.com
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Microbiology Indonesia
Published by Perhimpunan Mikrobiologi Indonesia
ISSN : 19783477     EISSN : 20878575     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology
Microbiology Indonesia provides a unique venue for publishing original researches in microbiology (espesially from Indonesian reseachers), and ensures that authors could reach the widest possible audience. Microbiology Indonesia publishes a wide range of research disciplines on bacteria, archaea, fungi, protozoa, and virus as well as biotechnology related to microbiology. Topics include (but are not limited to): -methods in microbiology, -bioprocess, -environmental microbiology, -food microbiology, -plant-microbe interaction, -animal-microbe interactions, -microbial community, -microbial genetics, -virology, -comparative and functional microbial genomics, -and gene expression in microbes.
Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi dan Bioteknologi Terapan
Articles 6 Documents
 1 
Search results for , issue "Vol. 12 No. 3 (2018): September 2018" : 6 Documents clear
Bacterial Population and Chemical Characteristics of Fermented Mandai Cempedak with Starter Induction
Microbiology Indonesia Vol. 12 No. 3 (2018): September 2018
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (722.65 KB) | DOI: 10.5454/mi.12.3.3

Abstract

Traditionally fermented foods can be improved by introducing starter and hygienic production. The study observes the changes in population of lactic acid bacteria (LAB), pH, polyphenolic levels, and antioxidant activity of spontaneous and L. casei induced mandai cempedak fermentation at 37 °C for seven days. The hygienic process included two steps boiling of inner skin of cempedak at 80-90 °C for 15 minutes. LAB and non-LAB growth were quantified with plate count. Phenolic substances were spectrophotometrically quantified. Gallic acid (GAE), tannic acid (TAE), and catechin (CE) were used as standards. DPPH method was employed to measure antioxidant activity. LAB dominated bacteria population during the course of fermentation. The LAB grew from 3,3±0,5 to 8,8±0,6 log cfu/ml for spontaneous fermentation and from 3,3±0,4 to 9,0±0,5 log cfu/mL for starter induced fermentation. The population of BAL in spontaneous and L. casei induced fermentation grew in almost similar fashion and can be approached by linear regression. The degree of acidity increased during the course of fermentation and achieving pH 3,5 at the sixth day of fermentation. Fermentation increased the phenolic contents both in spontaneous and L. casei induced fermentation, and resulting in enhancing the antioxidant activity. The phenolic contents, except total tannins, were higher in starter induced fermentation, thus lowering IC50 inhibitions of DPPH reduction. Hence, L. casei produced fermented products with better antioxidant activity in comparison to spontaneously fermented products. From these parameters, L. casei was successfully used as starter for mandai cempedak and optimum fermentation at 37 °C was 6 days.
The Growth of Leptolyngbya HS-16 and HS-36 on 35oC with pH Variation
Microbiology Indonesia Vol. 12 No. 3 (2018): September 2018
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1108.082 KB) | DOI: 10.5454/mi.12.3.1

Abstract

The observation of Leptolyngbya growth on temperature 35oC with initial pH variation had been done. The study was descriptive research. The study aimed to determine the best initial growth pH for Leptolyngbya HS (Hot Spring)-16 and HS-36. Leptolyngbya HS-16 was isolated from Pancar Mountain hotspring, while Leptolyngbya HS-36 was isolated from Maribaya hot spring.  The acidity (pH) of Pancar mountain and Maribaya hot spring was 7. Each strain was grown in Blue Green medium number 11 (BG-11) with variation initial pH (6, 7, 8 and 9) and incubated on 35 oC. Parameters was wet biomass weight of Leptolyngbya in each strain. Observation were made on 15 days with 11 sampling. From the observation, the average of wet biomass weight of Leptolyngbya HS-16 was obtained at pH 6 (0,0295 g/L), pH 7 (0,0404 g/L), pH 8 (0,03825 g/L), and pH 9 (0,02735 g/L), meanwhile Leptolyngbya HS-36 was obtained at pH 6 (0,02905 g/L), pH 7 (0,01995 g/L), pH 8 (0,05345 g/L) and pH 9 (0,05995 g/L) on the 15th day. The results of 15 days observation showed that the best initial pH for growing Leptolyngbya HS-16 is 7, while Leptolyngbya HS-36 is 9. From this study it could be seen that Leptolyngbya HS-16 and HS-36 could be cultured with alkaline condition. 
Isolation and Urease Activity Test of Bacteria for Calcium Carbonate (Calcite) Precipitation (Biocementation) in Soil HANIES AMBARSARI; AFLAKHUR RIDLO
Microbiology Indonesia Vol. 12 No. 3 (2018): September 2018
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (605.545 KB) | DOI: 10.5454/mi.12.3.2

Abstract

The use of bacterial calcium carbonate (calcite) precipitation (biocementation) has recently become popular as a ground-improvement technique. Ureolytic bacteria having highly urease activities were known to have important roles in calcium carbonate precipitation process. One of our research objectives is to isolate and to select as many as possible such ureolytic bacteria from Indonesian soils to be further utilized for calcium carbonate (calcite) precipitation process in the soil for strengthening the soil structure. Isolation was performed anaerobically in selective media containing 40% urea. Four isolates with different morphologies were purified and coded as TK1, TK2, TK3, and TK4. Each of them was tested for its urease activity either as a pure culture or as a mixture of several cultures. The urease activity was measured based on the ammonia concentration produced in the growth media up to 7 x 24 hours. It was known that isolate TK4 had the highest urease activity on week 6, whilst a mixture of isolate cultures coded as TKC did not show a better urease activity than the isolate TK4. Hence, it could be concluded that the isolate TK4 was the best candidate to be used for further research on the calcium carbonate (calcite) precipitation process (biocementation) to strengthen the soil structure.
The Administration of Pseudoalteromonas piscisida 1UB through Artemia sp. to Enhance Growth Performance, Immune Response and Resistance of White Shrimp (Litopenaeus vannamei) Larvae against Vibrio harveyi
Microbiology Indonesia Vol. 12 No. 3 (2018): September 2018
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (518.59 KB) | DOI: 10.5454/mi.12.3.4

Abstract

This study aimed to evaluate the effectiveness of the supplementation of Pseudoalteromonas piscisida 1UBthrough Artemia sp. to enhance the growth performance, immune response and the resistance of white shrimp (Litopenaeus vannamei) larvae to the infection of Vibrio harveyi. The natural feed given to the white shrimp larvae was Artemia sp. enriched with P. piscisida 1UBR at concentrations of 106 CFU mL-1, 107 CFU mL-1, 108 CFU mL-1 and a control (Artemia sp. without any enrichment). The experimental shrimps (0.25±0.02 mg shrimp-1) were reared in the aquarium (25 × 20 × 30 cm) containing 4 L sea water with a stocking density of 30 shrimps L-1. The experimental shrimps were fed the experimental feed from mysis 3 to PL12, and after that they were challenged with V. harveyi (107 CFU mL-1)through an immersion method. The results of this study revealed that the administration of Artemia sp. enriched with P. piscisida 1UB could improve the survival, daily growth rate and absolute growth of length of white shrimp larvae. The activities of protease, lipase and amylase of white shrimp larvae treated with probiotic were higher (p<0.05) than those of the control. After the challenge test, white shrimp larvae treated with probiotic also had better survival and immune response (total hemocyte count, phagocytic activity, phenoloxidase activity and respiratory burst activity) than those of the the positive control. The best results were obtained in the probiotic application with a concentration of 108 CFU mL-1.
The Potency of Aluminum Hydroxide Nanoparticles for Dengue Subunit Vaccine Adjuvant SABAR PAMBUDI; ETIK MARDLIYATI; SILMI RAHMANI; DAMAI RIA SETYAWATI; TIKA WIDAYANTI; ANGELINA GILL; ASRI SULFIANTI; WHINIE LESTARI
Microbiology Indonesia Vol. 12 No. 3 (2018): September 2018
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (984.8 KB) | DOI: 10.5454/mi.12.3.5

Abstract

The potency of aluminum hydroxide as an adjuvant in vaccine development is considered to depend on its particle size. In previous studies, we have successfully prepared two size particle, micro, and nano, aluminum hydroxide gel (alum) adjuvants. The potency of those particles as a candidate of adjuvant is needed to be characterized. In this study, we formulated our adjuvants with purified DENV3 pre Membrane Envelope (prM-E) recombinant protein and evaluated the induction of nitric oxide level in mouse macrophage RAW 264.7 cells. We prepared the alum adjuvant by precipitation-homogenization methods with an agitation rate at 11,000xg. Secreted prM-E recombinant protein was collected from Pichia pastoris X-33 fermentation which produced using bioreactor. Recombinant protein purification was carried out by anion exchange chromatography followed with size exclusion chromatography. The purified prM-E recombinant protein was observed as a single band around 70 -1k Da with a concentration of 105 mg mL . Complex nanoparticles alum with prM-E protein significantly (p<0.05) induced the nitric oxide level. Further analysis should be conducted in order to discover the detail molecular mechanism of nanoparticle alum adjuvant, recombinant protein, and cellular immune response.
ITA REGISTRATION FORM AND BACK COVER Is Helianti
Microbiology Indonesia Vol. 12 No. 3 (2018): September 2018
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5454/mi.12.3.%p

Abstract

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