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Microbiology Indonesia
Published by Perhimpunan Mikrobiologi Indonesia
ISSN : 19783477     EISSN : 20878575     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology
Microbiology Indonesia provides a unique venue for publishing original researches in microbiology (espesially from Indonesian reseachers), and ensures that authors could reach the widest possible audience. Microbiology Indonesia publishes a wide range of research disciplines on bacteria, archaea, fungi, protozoa, and virus as well as biotechnology related to microbiology. Topics include (but are not limited to): -methods in microbiology, -bioprocess, -environmental microbiology, -food microbiology, -plant-microbe interaction, -animal-microbe interactions, -microbial community, -microbial genetics, -virology, -comparative and functional microbial genomics, -and gene expression in microbes.
Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi dan Bioteknologi Terapan
Articles 5 Documents
 1 
Search results for , issue "Vol. 15 No. 2 (2021): June 2021" : 5 Documents clear
Bacterial Population Dynamics of Natural Fermentation of Sumbawa Mare’s Milk Using Metagenomic Approach Yoga Dwi Jatmiko; Aditya Ragil Suharto; Irfan Mustafa; Siska Aditya
Microbiology Indonesia Vol. 15 No. 2 (2021): June 2021
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (477 KB) | DOI: 10.5454/mi.15.2.2

Abstract

This study aimed to assess the changing of bacterial density and the physicochemical aspects during natural fermentation of Sumbawa mare’s milk, and to evaluate the dynamics of bacterial population during the natural fermentation using metagenomic approach. Mare’s milk sample obtained from Regency of Dompu were fermented for 60 days. On the day 0, 7, 15, 30 and 60 mare milk sample were collected for further analysis, such as bacterial density enumeration, nutrition content, physical properties of the milk, and total DNA isolation. The total DNA samples obtained were analyzed using next generation sequencing. The density of lactic acid bacteria was decreased along with fermentation periods. Meanwhile, the density of aerobic bacteria on was relatively fluctuated. The physicochemical content of mare’s milk also changed during fermentation periods. Carbohydrate content and total sugar was decrease along with the decreasing of pH value. Moreover, the lipid content increase, and the protein content was fluctuated. The changing in physical properties such as whey color, acidity and gas was observed until the end of mare’s milk natural fermentation process. Using metagenomics analysis, the bacterial diversity from each sample periods categorized as low because of the dominance of Lactobacillus helveticus until the end of the fermentation. Lactobacillus helveticus as a member of LAB did not grow on isolation media on the late stage of fermentation periods (day-60). The presence of uncultivable bacteria can be detected with metagenomic approach, fulfilling the limited information on the bacterial composition of fermented Sumbawa mare’s milk products.
Isolation and Identification of Hg-Resistant Bacteria as Bioremediator Agents and Their Potential in Reducing Mercury Contamination SORAYA FITRIA NASIR; ANI M. HASAN; ARYATI ABDUL; YULIANA RETNOWATI
Microbiology Indonesia Vol. 15 No. 2 (2021): June 2021
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (641.694 KB)

Abstract

This study aims to describe Hg-resistant bacteria in soil contaminated with gold mining waste and its ability to reduce mercury contamination. This research was initiated by taking soil samples at the gold processing plant in Ilangata Village, Anggrek District, North Gorontalo Regency. Then the research was carried out at the Microbiology Laboratory of the Biology Department, Faculty of Mathematics and Natural Sciences. Mercury analysis was carried out at the Laboratory of Fisheries Product Quality Development and Testing (LPPMHP) Gorontalo Province and bacterial identification was carried out at the HumRC Research Unit, Faculty of Medicine, Hasanuddin University. The study was conducted from July to October 2020. This study used a descriptive method. The parameters observed were the types of Hg resistant bacteria and the ability of the bacteria to reduce mercury contamination. Data were analyzed descriptively. The results showed that there were four bacterial isolates on the soil contaminated with mercury at 4.5 ppm. Two of them could not be resistant to levels of 10 ppm mercury. However, these four isolates had the ability to reduce mercury levels by 99%. Based on the reconstruction of the phylogenetic tree, bacterial isolate 01 has a close relationship with Stenotrophomonas sp. SB67, while bacterial isolate 02 had a close relationship with Enterobacter cloacae strain CM 1 16S, bacterial isolate 03 was identified as Stenotrophomonas maltophilia and bacterial isolate 04 was a bacterium from the genus Bacillus which was closely related to Bacillus albus strain SQ30 16S.
Potential Zoonotic Faecal Bacteria from Sunda Porcupine (Hystrix javanica) and Their Antimicrobial Resistance Profiles Sarsa Nisa; Rifka A. Safitri; Nurul Inayah; Achirul Nditasari; Susiana Purwantisari; Rejeki Ferniah; Anang Achmadi; Taufiq Nugraha; Sugiyono Saputra
Microbiology Indonesia Vol. 15 No. 2 (2021): June 2021
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (632.101 KB) | DOI: 10.5454/mi.15.2.4

Abstract

Sunda porcupine (Hystrix javanica) is one of the Indonesian endemic species which is often sought after for their meat. Although it is becoming increasingly popular for extreme culinary, information regarding biological risks arising from this wildlife is very limited. This study aimed to assess potential zoonotic faecal bacteria carried by Sunda porcupine with culture-dependant approach and to investigate whether antimicrobial resistant isolates can be found in wildlife. A total 22 faecal samples were collected from captive Sunda porcupine and tested for the presence of pathogens in selective media for Salmonella and Listeria. After inoculating the samples in Rappaport-Vassiliadis (RV) Salmonella enrichment broth, two samples (9%) were regarded as positive for Salmonella in this presumptive test which indicated by growth black colonies on xylose lysine deoxycholate (XLD) agar. Meanwhile, the presence for Listeria was presumptively positive in all samples (100%), indicated by black colour appearance in Listeria isolation transwab. In total, 38 bacterial isolates were successfully purified, preserved and subjected for antimicrobial susceptibility testing (AST) by disk diffusion method. Resistance to ceftriaxone (3rd generation cephalosporins) was not detected while resistance to one or two antimicrobials was observed in seven isolates. Further, 16S rRNA bacterial identification was performed for selected isolates and based on sequence similarity on GenBank® databases and phylogenetic tree construction, those isolates were denoted as Pseudomonas xinjiangensis (XG4.4), Shigella sonnei (XD8.2 and G11.3), Proteus mirabilis (XH3.3, H4.2, and E1.2) and Klebsiella quasipneumoniae subsp. similipneumoniae (XF4.2). All identified isolates were sensitive to amikacin, amoxicillin-clavulanic acid, cefoxitin and ceftriaxone, except for one isolate Shigella sonnei (XD8.2) which was resistant to cefoxitin. Further research to confirm the pathogenicity of the isolates is still needed but based on these results, we support the hypothesies that Sunda porcupine is potential as a reservoir pathogenic bacteria and preventive measures are crucial to prevent transmission when processing this bushmeat.
Immunogenicity of Recombinant DNA Vaccine Encoding Non-Structural Protein-1 Dengue Virus Serotype-2 in Balb/c Mice Elitha Pulungan
Microbiology Indonesia Vol. 15 No. 2 (2021): June 2021
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (482.649 KB) | DOI: 10.5454/mi.15.2.4

Abstract

Background: Dengue Hemorrhagic Fever (DHF) is an infectious disease caused by the dengue virus (DENV) which spread widely in tropical and subtropical regions of the world. DENV is a single-positive strand RNA virus with a genome size of ± 11kb which encodes three structural proteins, seven non-structural proteins, and two untranslated regions (UTR). The non-structural protein-1 (NS1) of DENV is known to have important role in dengue pathogenesis also promising to be developed as dengue vaccine. Lately, novel vaccine approach by DNA immunization have given new perspective for a safe, stable, and immunogenic vaccine platform. Previously, we have successfully construct DNA vaccine encoding NS1 protein of DENV2 (pUNS1) which express recombinant NS1 protein in-vitro. Thus, in this current study the ability of pUNS1 to induce humoral immune response will be further analyzed by in mice immunization. Methods: Sixteen BALB/c mice aged of 4 weeks were immunized 3 times with 100 µg of pUNS1 or pUMVC4a on 2 week time interval. Blood sampling was carried out just before immunization and termination was done 2 week after last immunization. Titer from individual mice sera against DENV-2 were measure with in-house ELISA. Results: IgG against NS1 protein of DENV2 titer from mice group immunized with recombinant pUNS1 shown high ELISA absorbancies, 5 times higher than pUMVC4a group. This result suggest the ability of pUNS1 to induce humoral immune response against NS1 DENV-2 in-vivo. Conclusion: Recombinant pUNS1 can induce humoral immune response in mice.
Single nucleotide polymorphism in the rpoB gene Mycobacterium tuberculosis from Papua-Indonesia and its impact on rifampicin resistance: A whole genome sequencing analysis Yustinus Maladan; Tri Wahyuni; Hana Krismawati
Microbiology Indonesia Vol. 15 No. 2 (2021): June 2021
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1764.866 KB) | DOI: 10.5454/mi.15.2.1

Abstract

In the antibiotic era, Tuberculosis (TB) drugs resistance especially Rifampicin (RIF) is highly reported around the world. Resistance of RIF is caused by the mutation of genes that associated with RIF receptor. The aims of this study are detecting the Single Nucleotide Polymorphism of Rifampicin resistant genes using Whole Genome Sequencing (WGS) and analysing the profile of protein changing caused by SNP. Twenty Mycobacterium tuberculosis culture samples were passed on WGS procedure and 19 samples were adequate to further bioinformatics analysis. Single Nucleotide Polymorphisms Analysis was done using TBprofiler. Based on TBProfiler, seventeen samples were resistant to rifampicin. The mutations that cause the resistance are S450L, D435Y, H445Y, 430P, Q432K. Other Single Nucleotide Polymorphisms H835R, V534M and R224C were also found. The H835R mutants are present together with the S450L, V534M with S450L mutants, and R224C with Q432K mutants. Native protein for RNA Polymerase Subunit β used was the result of separation from the crystal structure of Mycobacterium tuberculosis H37Rv RNA polymerase (PDB: 5UHB). Binding affinity RIF to RNA Polymerase Subunit β calculated using AutoDock vina. Construction of mutant 3D structures using FoldX5. From the analysis, it was found that seventeen samples were resistant to rifampicin and two samples did not contain SNP which could cause resistance to rifampicin.

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