cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
Iman Rusmana
Contact Email
rusmana13@yahoo.com
Phone
+62217560536
Journal Mail Official
microbiology.indonesia@gmail.com
Editorial Address
kPERHIMPUNAN MIKROBIOLOGI INDONESIA (SeKretariat PERMI), Gedung 10.2 Indonesian Life Sciences Center (ILSC), Zona Bisnis Teknologi Puspiptek, Jalan Raya Serpong - Bogor Gunung Sindur, Jawa Barat 16340, Indonesia. Email: microbiology.indonesia@gmail.com
Location
Kota tangerang,
Banten
INDONESIA
Microbiology Indonesia
Published by Perhimpunan Mikrobiologi Indonesia
ISSN : 19783477     EISSN : 20878575     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology
Microbiology Indonesia provides a unique venue for publishing original researches in microbiology (espesially from Indonesian reseachers), and ensures that authors could reach the widest possible audience. Microbiology Indonesia publishes a wide range of research disciplines on bacteria, archaea, fungi, protozoa, and virus as well as biotechnology related to microbiology. Topics include (but are not limited to): -methods in microbiology, -bioprocess, -environmental microbiology, -food microbiology, -plant-microbe interaction, -animal-microbe interactions, -microbial community, -microbial genetics, -virology, -comparative and functional microbial genomics, -and gene expression in microbes.
Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi dan Bioteknologi Terapan
Articles 398 Documents
 15   16   17   18   19 
Lactobacillus fermentum LLB3 improves antioxidant activity of bitter melon (Momordica charantia)
Microbiology Indonesia Vol. 12 No. 2 (2018): June 2018
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (466.353 KB) | DOI: 10.5454/mi.12.2.5

Abstract

Oxidative stress plays a major role in the pathogenesis and progression of diabetes. Functional foods with an antioxidant effect can repress oxidative stress. Momordica charantia (bitter melon) contains substances with antidiabetic properties such as charantin, vicine, and polypeptide-p, as well as other unspecific bioactive components such as antioxidants. Lactobacillus fermentum LLB3 isolated from bamboo shoot pickle  was used to ferment bitter melon juice. The aim of this study was to evaluate changes in antioxidant activity of bitter melon juice during fermentation. Study has been carried out by fermenting bitter melon juice with L. fermentum LLB3. The free radical scavenging activity of the phenolics were done using 2,2-diphenyl- 1-picrylhydrazyl (DPPH).  Antioxidant activity of bitter melon juice increased during 24 hours fermentation. In addition, the sugar content and pH  decreased compared with the baseline value. The fermentation of bitter melon juice by L. fermentum LLB3 increased its antioxidant activity. These result suggest that fermented bitter melon juice is a promising agent for diabetes management.
Identification and Characterizations of Potential Indigenous Endophytic Bacteria which had Ability to Promote Growth Rate of Tomato and Biocontrol Agents of Ralstonia solanacearum and Fusarium oxysporum fsp. solani
Microbiology Indonesia Vol. 11 No. 4 (2017): December 2017
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (638.551 KB) | DOI: 10.5454/mi.11.4.2

Abstract

Among Plant Growh Promoting Rhizobacteria (PGPR) groups, endophytic bacteria considered as one of the options to control vascular wilt disease because of its ability to live and colonized internal roots of plants without causing any damages. Our previous research had screened 9 isolates which had best ability to promote growth rate and increase yields of tomato and biocontrol agents of R. solanacearum and Fusarium oxysporum f.sp solani in planta condition. In order to know its abilities, those isolates need to be characterized. This research purposedto characterize those isolates abilities to produce IAA, phosphate solubilizing, siderophore production, cyanide production, NH3 production, and ability to colonize endophytically and identified the isolates using 16S rRNA. Result shown that all isolates can produce IAA, where TLE1.1 produce highest IAA concentration (42.5 ppm). Isolates E1AB1.3, TLE 1.1 and TLE2.2 can dissolved phosphate. None of the isolates produced HCN and NH3. Only TLE 2.3 isolate can produce siderophore. All of 9 isolates were identified using 16S rRNA gene using 27F and 1492R primers. All isolates were identified as different species, i.e. Bacillus toyonensis strain BCT-7112 (EPL1.1.3), Serratia nematodiphila strain DZ0503SBS1 (TLE2.3), Bacillus anthracis strain ATCC 14578 (EPL1.1.4), Bacillus cereus ATCC 14579 (TLE1.1), Bacillus cereus strain JCM 2152 (SNE2.2), Enterobacter cloacae subsp. dissolvens strain ATCC 23373 (E1.AB1.2), Serratia marcescens strain NBRC 102204 (E1AB2.1), Klebsiella michiganensis strain W14 (TLE2.2), and Chryseobacterium rhizoplanae strain JM-534 (KLE3.3).
ITA REGISTRATION FORM AND BACK COVER Is Helianti
Microbiology Indonesia Vol. 11 No. 3 (2017): September 2017
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1661.804 KB) | DOI: 10.5454/mi.11.3.%p

Abstract

ITA REGISTRATION FORM AND BACK COVER
Microbiology Indonesia Vol. 11 No. 2 (2017): Juni 2017
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1661.804 KB) | DOI: 10.5454/mi.11.2.%p

Abstract

Detection of Antibody to Burkholderia pseudomallei in Captive and Wild Macaquesin West Java and Bali, Indonesia VINCENTIUS ARCA TESTAMENTI; DIAH ISKANDRIATI; ARIS TRI WAHYUDI; JOKO PAMUNGKAS
Microbiology Indonesia Vol. 12 No. 1 (2018): March 2018
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (500.83 KB) | DOI: 10.5454/mi.12.1.4

Abstract

Melioidosis is an emerging zoonotic disease caused by the saprophytic Burkholderia pseudomallei, which infects human and a wide range of animal species. Melioidosis may lead to septicemia and pneumonia in human patients, which can be fatal if the patient is not treated accordingly. The disease is spread in tropical areas and is highly endemic to Southeast Asia and Northern Australia. However, melioidosis is poorly reported in Indonesia, especially in the veterinary field. This research provides serological evidence of antibodies to B. pseudomallei in both captive and wild nonhuman primates. Plasma samples were taken from a total of 390 monkeys in captivities and wild habitats in West Java and Bali, Indonesia. Enzyme-linked immunosorbent assay (ELISA) showed that the seroprevalence was 42.21% for Macaca fascicularis and 43.59% for Macaca nemestrina. Furthermore, the seroprevalence was 53.41% for captive macaques and 17.83% for wild macaques. The findings showed that exposure to B. pseudomallei happened in both captive and wild macaques. Based on this serosurveillance results, further studies such as comprehensive culture and clinical study are required to discover the clinical burden of the disease in nonhuman primates.
Molecular Identification of Thermally-tolerant Symbiotic Dinoflagellates from Hard Coral (Scleractinia) in Biawak Island, Indonesia
Microbiology Indonesia Vol. 11 No. 4 (2017): December 2017
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5454/mi.11.4.3

Abstract

Symbiodinium is phototrophic dinoflagellates that occur as endosymbionts in various marine invertebrates and protists, forming mutualistic symbiosis with their hosts. The composition of Symbiodinium populations may also play an important role in the tolerance or sensivity of corals towards bleaching. Therefore, this research aims to identify thermally-tolerant Symbiodinium of scleractinian corals in Biawak Island using molecular techniques. Sampling was carried out from Acropora sp and Porites sp, from the depth of 3-5 meters. Symbiodinium was TMisolated through metagenomic approach using ZR Soil Microbe DNA Kit and 28S nrDNA gene was amplified TMusing Polymerase Chain Reaction (PCR). The resulted sequences were processed using BioEdit software and TMMEGA 5.2 for phylogenetic tree construction. The results indicated that isolates of ZX-ACP-ST.1 and ZXPORITES-ST.3 have similiarity to registered sequences of Symbiodinium thermophilum with identity value of TM99% and 98%. BLAST analysis of ZX-PORITES-ST.2 isolate indicated high identity (99%) to sequences of Symbiodinium sp. clade C while ZX-PORITES-ST.3 isolate has 99% similiarity to Symbiodinium sp. CG8. Phylogenetic analysis using UGPMA method showed that all isolates had a very close relationship and thought to have come from Symbiodinium clade C.
Potential Degradation of SARA (Saturated, Aromatics, Resinics, Asphaltenes) Fractions of Crude Oil by Reservoir Indigenous Bacteria from South Sumatera
Microbiology Indonesia Vol. 11 No. 4 (2017): December 2017
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (616.247 KB) | DOI: 10.5454/mi.11.4.5

Abstract

MEOR (Microbial Enhanced Oil Recovery) technology utilizes metabolic activity of microorganisms such as degradation of hydrocarbon fractions which alters oil characteristics to facilitate and increase oil recovery from reservoir. This research focused on isolation of indigenous hydrocarbonoclastic bacteria that were capable of degrading SARA (Saturated, Aromatic, Resinic, Asphaltenes) fractions of crude oil to be utilized in MEOR. Sequential isolation of hydrocarbonoclastic bacteria were conducted using Nutrient Broth and Stone Mineral oSalt Solution medium supplemented with 2% (v/v) crude oil and 0.1% (w/v) yeast extract and incubated at 50C, 120 rpm agitation. Isolates retrieved were screened based on its activity to degrade crude oil, indicated by resazurin assay result. Physical and chemical characteristics of crude oil altered by selected isolates were observed using column chromatography, biometric test, GC-MS analysis, IFT and viscosity measurements. Isolates with the best degradation activity were identified with 16S rRNA gene sequencing. Among thirty-one bacterial isolates obtained from sequential isolation, six isolates exhibited high oil degrading ability. SARA assay showed degradation activity of those isolates to each of SARA fraction were around 6-70%.  This degradation was followed by significant CO production ranging from 2000-4000 mg (value of p<0.05). Microbial 2 degradation activity exhibited changes in chemical and physical characteristics of hydrocarbons showed by changes in composition of SARA fraction, decreased viscosity and IFT of crude oil 17-31%. This research identified three isolates with the best hydrocarbon fraction degrading ability were identified as different strain of Bacillus licheniformis and confirmed their high potential to be utilized in MEOR technology.
Isolation and Identification of Ethanol and Glucose Tolerance Yeasts Strain from Tacca leontopetaloides
Microbiology Indonesia Vol. 11 No. 4 (2017): December 2017
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5075.6 KB) | DOI: 10.5454/mi.11.4.4

Abstract

The research aimed to isolate and characterize indigenous yeast strain from Tacca leontopetaloides with respect to the ethanol and glucose tolerance ability. Research done experimentally and the data analyzed descriptively. Yeasts isolated from 1g Tacca leontopetaloides grown at modified Potato Dextrose Agar/PDA (Oxoid Ltd.) with 3% Yeasts Extract/YE (Kraft Foods) and 10 ppm amoxicillin addition. Yeasts-like colony was tested in the ability to tolerate ethanol and glucose contents by grown on modified Nutrient Broth/NB (Oxoid Ltd.) with 3% Yeasts Extract/YE (Kraft Foods) and 10 ppm amoxicillin then added with glucose monohydrate (10%, 20%, 30%) or ethanol (10%, 20%, 30%) and incubated for 72h at ambient (23-28°C). Optical density (OD) was read for UV absorbance at 600 nm using UV-Vis spectrophotometer every 24h until 72h. The strain of best isolate with the ability to tolerate high ethanol and glucose contents were identified by the sequence analysis of ITS (Internal Transcribed Spacer) region using primers ITS1 (5′-TCCGTAGGTGAACCTGCGG-3′) and ITS4 (5′-TCCTCCGCTTATTGATATGC-3′). The sequencing was performed at Macrogen Inc. (Seoul, South Korea), and the sequences was compared with the GenBank database using BLAST (Basic Local Alignment Search Tools) algorithm (http//:www.ncbi.nlm.nih.gov/BLAST/). Results shown there are four yeasts-like isolate and the TK1 isolate showed the best ethanol tolerance ability with highest OD at 30% ethanol concentration (0.486) and the highest OD at 30% glucose concentration (1.732). The species identification identified the TK1 isolate as 99% identical with Candida natalensis (ITS1) and 100% identical with Candida quercitrusa (ITS4).
Bacterial leaching of an Indonesian complex copper sulfide ore using an iron-oxidizing indigenous bacterium
Microbiology Indonesia Vol. 12 No. 1 (2018): March 2018
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2002.316 KB) | DOI: 10.5454/mi.12.1.1

Abstract

The bioleaching of an Indonesian complex copper sulfide ore was studied in shake flasks over a period of 14 days using an iron-oxidizing indigenous bacterium at room temperature (28 oC) and various pulp densities (5% and 20%). The bioleaching suspensions were periodically analyzed for Cu and Fe concentrations as well as Eh, pH and DO values. Cu bioleaching efficiencies at 5% pulp density were higher than those at 20% pulp density, which correlated with Fe concentration in solution. Over a period of 14 days, the pH of bioleaching suspension was in the range of 5 ~ 9, indicating that Cu bioleaching was greatly influenced not only by proton H+ dan ferric ion but also by extracellular polymeric substances (EPS) generated by the bacterium. The current study may improve our better understanding on the bacterial action for bioleaching of complex copper sulfide ores that remains debated so far as refractory ores.
ITA REGISTRATION FORM AND BACK COVER
Microbiology Indonesia Vol. 11 No. 4 (2017): December 2017
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1403.19 KB) | DOI: 10.5454/mi.11.4.%p

Abstract

Page 17 of 40 | Total Record : 398

 15   16   17   18   19 

Filter by Year

2007 2023


Reset
Filter By Issues
All Issue Vol. 17 No. 2 (2023): June Vol. 17 No. 1 (2023): March Vol. 16 No. 2 (2022): December Vol. 16 No. 1 (2022): March Vol. 15 No. 3 (2021): September 2021 Vol. 15 No. 2 (2021): June 2021 Vol. 15 No. 1 (2021): March 2021 Vol. 15 No. 4 (2021): December Vol. 14 No. 4 (2020): December 2020 Vol. 14 No. 3 (2020): September 2020 Vol. 14 No. 2 (2020): June 2020 Vol. 14 No. 1 (2020): March 2020 Vol. 13 No. 4 (2019): December 2019 Vol. 13 No. 3 (2019): September 2019 Vol. 13 No. 2 (2019): June 2019 Vol. 13 No. 1 (2019): March 2019 Vol. 12 No. 3 (2018): September 2018 Vol. 12 No. 2 (2018): June 2018 Vol. 12 No. 1 (2018): March 2018 Vol. 11 No. 4 (2017): December 2017 Vol. 11 No. 3 (2017): September 2017 Vol. 11 No. 2 (2017): Juni 2017 Vol. 11 No. 1 (2017): March 2017 Vol. 10 No. 4 (2016): December 2016 Vol. 10 No. 3 (2016): September 2016 Vol. 10 No. 2 (2016): June 2016 Vol. 10 No. 1 (2016): March 2016 Vol. 9 No. 4 (2015): December 2015 Vol. 9 No. 3 (2015): September 2015 Vol. 9 No. 2 (2015): June 2015 Vol. 9 No. 1 (2015): March 2015 Vol. 8 No. 4 (2014): December 2014 Vol. 8 No. 3 (2014): September 2014 Vol. 8 No. 2 (2014): June 2014 Vol. 8 No. 1 (2014): March 2014 Vol. 7 No. 4 (2013): November 2013 Vol. 7 No. 3 (2013): September 2013 Vol. 7 No. 2 (2013): June 2013 Vol. 7 No. 1 (2013): March 2013 Vol. 6 No. 4 (2012): December 2012 Vol. 6 No. 3 (2012): September 2012 Vol. 6 No. 2 (2012): June 2012 Vol. 6 No. 1 (2012): March 2012 Vol. 5 No. 4 (2011): December 2011 Vol. 5 No. 3 (2011): September 2011 Vol. 5 No. 2 (2011): June 2011 Vol. 5 No. 1 (2011): March 2011 Vol. 4 No. 3 (2010): December 2010 Vol. 4 No. 2 (2010): August 2010 Vol. 4 No. 1 (2010): April 2010 Vol. 3 No. 3 (2009): December 2009 Vol. 3 No. 2 (2009): August 2009 Vol. 3 No. 1 (2009): April 2009 Vol. 2 No. 3 (2008): December 2008 Vol. 2 No. 2 (2008): August 2008 Vol. 2 No. 1 (2008): April 2008 Vol. 1 No. 3 (2007): December 2007 Vol. 1 No. 2 (2007): August 2007 Vol. 1 No. 1 (2007): April 2007 More Issue