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Iman Rusmana
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kPERHIMPUNAN MIKROBIOLOGI INDONESIA (SeKretariat PERMI), Gedung 10.2 Indonesian Life Sciences Center (ILSC), Zona Bisnis Teknologi Puspiptek, Jalan Raya Serpong - Bogor Gunung Sindur, Jawa Barat 16340, Indonesia. Email: microbiology.indonesia@gmail.com
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INDONESIA
Microbiology Indonesia
Published by Perhimpunan Mikrobiologi Indonesia
ISSN : 19783477     EISSN : 20878575     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology
Microbiology Indonesia provides a unique venue for publishing original researches in microbiology (espesially from Indonesian reseachers), and ensures that authors could reach the widest possible audience. Microbiology Indonesia publishes a wide range of research disciplines on bacteria, archaea, fungi, protozoa, and virus as well as biotechnology related to microbiology. Topics include (but are not limited to): -methods in microbiology, -bioprocess, -environmental microbiology, -food microbiology, -plant-microbe interaction, -animal-microbe interactions, -microbial community, -microbial genetics, -virology, -comparative and functional microbial genomics, -and gene expression in microbes.
Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi dan Bioteknologi Terapan
Articles 398 Documents
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The use of Sprout as Precursor for the Production of Indole Acetic Acid by Selected Plant Growth Promoting Rhizobacteria Grown in the Fermentor
Microbiology Indonesia Vol. 10 No. 4 (2016): December 2016
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1036.612 KB) | DOI: 10.5454/mi.10.4.3

Abstract

Indole-3-acetic acid (IAA) is the main member of the auxin family that controls many important physiological processes in plant. Such beneficial IAA that produced by plant growth promoting rhizobacteria (PGPR), enhances plant growth and was believed to increase the access to more nutrients in the soil. The precursor for syntetizing IAA is tryptophan, and it was also found in the sprout or other sources of protein. The aim of this study was to investigate the best bacteria and growth medium supplemented with extract of bean sprout or fish meal as the sources of precursor for the IAA production. Several bacterial isolates were screened for highest IAA production. IAA production was measured with High Performance Liquid Chromatography. All of isolates were able to produce IAA and isolates PS1 was selected for the further assay by cultivating under fermentor system. Sequencing of 16S rDNA of PS1 isolate indicated as Acinetobacter sp. The result showed that the highest IAA production during fermetation was 62,428 ppm found in under medium supplemented with mung bean sprout extracts grown in fermentor, after 24 hours incubation.
Selection and Bioassay of Azotobacter sp. Isolates to Improve Growth of Chili (Capsicum annum L.) on Entisols in Ambon REGINAWANTI HINDERSAH; PRIYANKA PRIYANKA; WILHELMINA RUMAHLEWANG; A MARTHIN KALAY
Microbiology Indonesia Vol. 10 No. 4 (2016): December 2016
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (677.784 KB) | DOI: 10.5454/mi.10.4.2

Abstract

Leafy vegetables contributes to the inflation rate in Ambon City due to low productivity in rainy season. Some vegetables are imported from other islands while importantvegetables such as local petsai (Brassica chinensis L.) and chili (Capsicum annum L.) are cultivated in low nitrogen soil, Entisols. Lack of nitrogen could be overcome by using inorganic fertilizeras well as biofertilzer. The soil can be inoculated with rhizobacteria, such as Azotobacter, to increase  the nitrogen uptake and improve the quality of vegetables. This research was conducted to isolate and select Azotobacter from rhizosphere of vegetables and to examine the effect of Azotobacter inoculation on chili-seedling growth and nitrogen uptake by using bioassay method. Azotobacter sp. was isolated in nitrogen-free Ashby’s Media. The bioassay was held in the green house with randomized block design experiment, which examined the combination of isolates and population of Azotobacter sp. on chili. Two best isolates which was selected based on pH, nitrogen content and cell viability were s2a10 (from petsai's rhizosphere) and c2a9 (from chili’s rhizosphere). Bioassay showed that Azotobacter inoculation followed by reduced NPK fertilizer doses had no effect on transplant dry weight and nitrogen uptake. All Azotobacter 8 -1inoculation except  10 CFU mL s2a10 maintain soil nitrogen although Azotobacter population in soil was slightly reduced. This showed that Azotobacter sp. potentially reduce the use of inorganic biofertilizer.
ITA REGISTRATION FORM AND BACK COVER
Microbiology Indonesia Vol. 10 No. 3 (2016): September 2016
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1346.674 KB) | DOI: 10.5454/mi.10.3.%p

Abstract

Analysis of Human Immune Response against Salivary Glands Protein Extract of Anopheles sundaicus. L in Malaria Endemic Area
Microbiology Indonesia Vol. 11 No. 1 (2017): March 2017
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (778.682 KB) | DOI: 10.5454/mi.11.1.4

Abstract

Malaria is an infectious disease caused by Plasmodium, which is transmitted by Anopheles mosquitoes as vectors. Malaria transmission begins when an infected mosquito takes blood meal from healthy human. Mosquitoes will release parasite and components of saliva into the host's body. Saliva contains components (proteins) that affect the host's hemostasis and immune respose, such as vasomodulator and immunomodulators. Imunomudulator could act as immunosuppressive factors that can suppress nonspecific immune system of the host and modulate the change of T helper 1 (Th1) toward T helper 2 (Th2) response, which is advantageous for malaria parasite to infect human host. This research wanted to evaluate human immune respons in endemic area against salivary gland protein extract (SGPE) from its major malaria vector i.e. Anopheles sundaicus (An. sundaicus). Analysis of human immune response was conducted quantitatively by ELISA (Enzyme Link Immunosorbend Assay) towards IgG from human sera samples after cross reacted with SGPE. The results showed that exposures to An. sundaicus were able to induce high levels of IgG. IgG anti salivary proteins of An. sundaicus is higher than the levels of IgG anti salivary proteins of Ae. aegypti. Furthermore, the age group 11-40 years with the highest bites probability, had the highest IgG levels compared to other age groups.
Effect of catfish’ (Clarias gariepinus) flour and oil with probiotic Enterococcus faecium IS-27526 based functional feed provision on bodyweight and C-reactive protein (CRP) of aged atherogenic female Cynomolgus monkey (Macaca fascicularis)
Microbiology Indonesia Vol. 10 No. 4 (2016): December 2016
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (780.912 KB) | DOI: 10.5454/mi.10.4.5

Abstract

The aim of the research was to study the effect of functional feed of catfish’ flour, oil and probiotic E. faecium IS-27526 based on bodyweight and CRP (C-reactive protein) of aged female Cynomolgus monkey (Macaca fascicularis). Nine aged female Cynolmolgus randomly divided into three groups. The age is determined by dentition, with bodyweight in a range of  2 – 4 kg. Animals were placed in individual cages in the position where they can interact audiovisually. Feed composition consists of sugar, egg, soy flour, wheat flour, sweet potato flour, butter, egg yolk flour, catfish’ flour and oil, and microencapsulated probiotic Enterococcus faecium IS-27526 and administered for 90 days. Evaluation of bodyweight (BW) and CRP were conducted. There is no significant effect (p>0.05) of experimental diets on bodyweight in each group. However, probiotic tends to delay the bodyweight gain. The bodyweight of cynomolgus in probiotic diet is shown more stable than others. There is no the effect of experimental diets on CRP which is marked by negative result of CRP test. Probiotic E. faecium IS-27526 is potential for bodyweight homeostasis regulation to reduce the risk of overweight and obesity.
Optimization of Lipases Production byBacillus licheniformis F11.4 using Response Surface Methodology
Microbiology Indonesia Vol. 10 No. 4 (2016): December 2016
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2073.575 KB) | DOI: 10.5454/mi.10.4.4

Abstract

Lipase is a lipids hydrolyze enzyme which are widely used in various industries such as chemical, pharmaceutical, food industries, and detergents. Bacillus licheniformis F11.4 is one of the bacteria withpotential source of lipase. This study aimed to obtain optimum production of lipase from B. licheniformis F11.4 by optimizing the composition of media and pH values with fish flour as a replacement for peptone andyeast extract based medium. Selection of the significant factors used a 2-level factorial design. The upper limit and lower limit of the selected factors was optimized using Central Composite Design (CCD) and thedata analysis was performed using the Response Surface Methodology (RSM). Fermentation was carried out in erlenmeyer at initial pH 8 and a temperature of 37 °C, using a shaker incubator at 150 rpm. A fermentation system for lipases production is considered optimal when its desirability value closes to 1. By using numerical optimization, an optimal medium could be obtained, i.e. consisting of OO:CPO 0.14 % (w/v) andfish flour 2% (w/v), at pH 8 and 150 rpm, which produced lipase with enzyme activity of 1.563 U mL-1 and protein level of 0.08 mg mL-1.Furthermore, the results are verified in the Erlenmeyer, working volume of 50 mL, pH = 8, T = 37 °C, agitation 150 rpm, t=18 hours, the activity of lipase and protein levels are 1.568 ± 0.014 U mL-1 and 0.072 ± 0.006 mg mL-1 respectively.The results showed that the optimum condition lipaseactivity was 1.568 U mL-1 so that the increase in the activity of only 75% compared to before optimization.
Studies for IAA (Indole-3-Acetic Acid) Production by Isolates H6 with Nitric Acid Mutation RAHAYU FITRIANI WANGSA PUTRIE; TIWIT WIDOWATI; HARMASTINI SUKIMAN
Microbiology Indonesia Vol. 11 No. 1 (2017): March 2017
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (502.555 KB) | DOI: 10.5454/mi.11.1.3

Abstract

Nitric acid mutations are known could be used for strain improvement. This research aimed to studies IAA production by nitric acid mutan were compared with wild type. Mutation were conducted with some different treatment time such as 0, 30, 60, 90 and 120 min subsequently it were measured for IAA production. Isolate H6 as -1wild type isolates were also molecularly identified. The wild strain exhibited 53.83 µg mL of IAA while the -1 -1. nitric acid mutan within a range 77.39 µg mL to 95.70 µg mL Isolates H6.60 exhibited the highest IAA -1 production which 39.87 µg mL higher were compared with wild-type. Based on 16S rRNA gene analysis, isolate H6 belonged to Lysobacter sp. ES2-22.
Cloning of Synthetic Lipase Gene from Rhizomucor miehei with Original Signal Peptide in Pichia pastoris
Microbiology Indonesia Vol. 11 No. 1 (2017): March 2017
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1263.49 KB) | DOI: 10.5454/mi.11.1.1

Abstract

Lipases (EC 3.1.1.3) are classified as hydrolases that hydrolyze lipids. These enzymes have potential application in biotechnology and industrial process. In previous study we have cloned the synthetic Rhizomucor miehei lipase gene using the vector pUC57 in Escherichia coli DH5α, but only found the very low enzymes activity. This study aimed to clone Rhizomucor miehei synthetic lipase gene into Pichia pastoris expression plasmid for lipase expression with the original signal peptide. A DNA fragment with the original signal peptide had been obtained by PCR, cut by Xho I and Xba I and then ligated into pPICZα A linearized with the same enzymes. The mixture of ligation, then was transformed into Escherichia coli DH5α. Zeocin-resistant transformants were selected and contained plasmid was analyzed by restriction enzymes analyses, PCR, and DNA sequenced. As the result, a Rhizomucor miehei lipase gene (RMlip) with the size of 1132 bp was successfully cloned to pPICZα A. The recombinant plasmid with the correct DNA sequence was transformed into Pichia pastoris X33. Cultivation of recombinant P. pastoris was carried out with the addition of 1.5% methanol every day with appropriate aeration. The recombinant lipase produced by Pichia pastoris X33 containing RMlip oin its chromosomal DNA had optimal temperature and pH 30 C and 9.0, respectively.
In Vitro Phytochemical and Inhibitory Potential Test of Bawang Hutan Bulb Extract (Eleutherine palmifolia) on Vibrio harveyi WAODE MUNAENI; ARMAN PARIAKAN; LAODE BAYTUL ABIDIN; MUNTI YUHANA
Microbiology Indonesia Vol. 11 No. 3 (2017): September 2017
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (945.507 KB) | DOI: 10.5454/mi.11.3.1

Abstract

The objectives of this study were to analyze phytochemical content of bawang hutan bulbs extract (Eleutherine palmifolia) and to test the inhibitory potential of bawang hutan bulbs extract on the growth of Vibrio harveyi bacteria at different doses. This study was conducted in March-May 2017 in Testing Laboratory of Fisheries and Marine Science Faculty of Halu Oleo University and Laboratory of Fish Health of Aquaculture Department of Fisheries and Marine Science Faculty and Laboratory of Biopharmaca of Bogor Agricultural University. Test parameter included: (1) Phytochemical test through the method of color visualization, (2) Inhibitory potential test using two methods namely agar diffusion and co-culture. Treatment of dose consisted of positive control/K+ (Chloramphenicol 30 mg/ml), negative control/K- (Sterile Aquadest) and treatment of extract included A (20 mg/ml), B (40 mg/ml), C (60 mg/ml), D (80 mg/ml). Qualitatively, result of phytochemical test showed that bawang hutan bulbs extract contained flavonoid, tannin, saponin, quinone, steroid and triterpenoid compounds. Result of inhibitory potential test indicated that treatment D obtained the highest inhibitory potential, while the minimum inhibitory potential was found in treatment A. The best co-culture test result was also found in treatment D, in which 24 hours after co-culture was performed, no V. harveyi colonies (total bacteria of 0 CFU/mL) were found. Bawang hutan bulbs extract in this study was able to inhibit the growth of V. harveyi.
Construction and Expression of Single Recombinant Peptide Surfactant for EOR Application CUT NANDA SARI; USMAN USMAN; RIESA KW ROHMAT; LENI HERLINA; KEN SAWITRI SULIANDRI; ONIE KRISTIAWAN; DWIYANTARI DWIYANTARI; TATI KRISTIANTI; SONY SUHANDONO
Microbiology Indonesia Vol. 11 No. 1 (2017): March 2017
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1420.163 KB) | DOI: 10.5454/mi.11.1.5

Abstract

Surfactant is generally synthetic chemical, which is effective and reliable. However, the chemicals usually did not degraded easily in the environment and could cause damage to the environment. The other possible alternative to produce surfactant is using genetic engineering in order to produce peptide based surfactant. In this research, peptide surfactant was produced using a gene construct which was created using overlapped polymerase chain reaction method (OE-PCR). PAGE analysis shows that single surfactant peptide construction can be expressed by induction of IPTG 1 mM and after at least twice sonication. This research proves that both two constructions have been successfully expressed by producing peptide in expected size (approximately 15 kDa).

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