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Microbiology Indonesia

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Published by Perhimpunan Mikrobiologi Indonesia

ISSN : 19783477 EISSN : 20878575 DOI : -

Core Subject : Health, Science,

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology

Microbiology Indonesia provides a unique venue for publishing original researches in microbiology (espesially from Indonesian reseachers), and ensures that authors could reach the widest possible audience. Microbiology Indonesia publishes a wide range of research disciplines on bacteria, archaea, fungi, protozoa, and virus as well as biotechnology related to microbiology. Topics include (but are not limited to): -methods in microbiology, -bioprocess, -environmental microbiology, -food microbiology, -plant-microbe interaction, -animal-microbe interactions, -microbial community, -microbial genetics, -virology, -comparative and functional microbial genomics, -and gene expression in microbes.

Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi dan Bioteknologi Terapan

Articles 398 Documents

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Phenotypic, Metabolic, and Genetic Diversity of the Indonesian Isolates of Rhizopus oligosporus CAHYA PRIHATNA; ANTONIUS SUWANTO
Microbiology Indonesia Vol. 1 No. 1 (2007): April 2007
Publisher : Indonesian Society for microbiology

Fifteen Rhizopus oligosporus isolates were isolated from a number of tempeh samples obtained from Mataram, Jember, and Bogor, Indonesia; and subjected for characterization based on phenotypic, metabolic, and genetic fingerprinting through internal transcribed spacer (ITS) regions and amplified fragment length polymorphism (AFLP). Based on the growth on solid medium, they can be divided into three groups. Firstly, isolates that produced thick mycelia, dumpy sporangiophore, and scarce spores in agar culture, the second group is isolates that produced thin mycelia, stretched sporangiophore, with abundant spores in agar culture. The third group that only comprises one isolate, FB-06, is morphologically intermediate of the first and the second groups. These characters correlated with their range of temperature tolerance. The first group is less tolerant to high temperature (45 oC) compared with the second group, and the third group is the most tolerant to temperature up to 45 oC. Metabolic fingerprinting showed a very high polymorphism. In general, the result may explain a correlation in which isolates obtained from the same locations shared similar patterns. There is no correlation found between metabolic fingerprints and their phenotypic fingerprints. Rhizopus oligosporus readily dominated the niche and utilized nearly all carbon sources given demonstrate the versatile nature of this fungus. ITS regions identification revealed single nucleotide polymorphisms in four representative isolates examined, whereas AFLP fingerprinting determined each of representative isolates as individually unique. Furthermore, this AFLP profile seemed to agree with their phenotypic characters..

Structural and Functional Analysis of FLAG Tagged-Subunit 8 of Yeast Saccharomyces cerevisiae Mitochondrial ATP Synthase I MADE ARTIKA
Microbiology Indonesia Vol. 1 No. 1 (2007): April 2007
Publisher : Indonesian Society for microbiology

Yeast mitochondrial ATP synthase is a multisubunit complex composed of at least 17 different subunits. Subunit 8 of yeast mitochondrial ATP synthase is a hydrophobic protein of 48 amino acids encoded by the mitochondrial ATP8 gene. Although ATP synthase from eukaryotes and prokaryotes shows a similar basic structure, no homologue of subunit 8 is found in prokaryotes such as Escherichia coli. Subunit 8 has three distinct domains; an N-terminal domain, a central hydrophobic domain and a C-terminal domain. In order to elucidate its structure and function, a set of nuclear genes encoding subunit 8 variants was designed to incorporate a FLAG tag at the C-terminus and a mitochondrial signal peptide at the N-terminus. Each gene was cloned into a yeast expression vector and then allotopically expressed in a yeast strain lacking endogenous subunit 8. Structural and functional analysis showed that the hydrophobic character of the central hydrophobic domain of subunit 8 is critical for the ATP synthase function. Subunit 8 is sensitive to charge manipulation at the C-terminus. The positively charged residues at the C-terminal domain are important for subunit 8 assembly and hence its function.

16S Ribosomal RNA-Based Analysis of Thermophilic Bacteria in Gedongsongo Hot Spring AGUSTINA LULUSTYANINGATI NURUL AMININ1; FIDA MADAYANTI; PINGKAN ADITIAWATI; . AKHMALOKA
Microbiology Indonesia Vol. 1 No. 1 (2007): April 2007
Publisher : Indonesian Society for microbiology

Denaturing gradient gel electrophoresis was used to identify the bacterial community at the Gedongsongo (WGS-2) hot spring. The bacterial samples were obtained from both culture dependent and independent strategies. Partial 16S rRNA genes were amplified by a set of primers to produce at around 400 bp fragments, including the highly variable V9 region of the 16S rRNA genes. The DGGE profiles showed that there were a few distinct bands, namely G1-G3, and G8-G12, which represent the predominant bacteria in natural habitat and the medium.Further analysis of these bands showed that most of them, except for G7, have a high homology to the 16S rRNA gene sequences of Thermus sp. As for G7, the highest homology was shown to unculturable bacteria. In addition to the distinct bands in DGGE, there were other three thin bands, namely G4, G5, and G6, which possibly represent non dominant microorganisms in the natural habitat, but could grow on GS-A medium. Further analysis of these bands showed that G6 has 80% similarity to the 16S rRNA of Burkholderia sp., while G4 and G5 have a high homology to each other but only contained 10-15% homology to the sequences of 16S rRNA from unculturable microorganisms. The phylogenetic analyses of the last organisms showed that there was branching from Burkholderia. From all the data obtained it was suggested that the WGS-2 hot spring was predominantly occupied by the genus Thermus. In addition, there were a few novel microorganisms found in the hot spring.

Effects of Temperature on Denitrifying Growth and Nitrate Reduction End Products of Comamonas testosteroni Isolated from Estuarine Sediment IMAN RUSMANA
Microbiology Indonesia Vol. 1 No. 1 (2007): April 2007
Publisher : Indonesian Society for microbiology

Predictions of seasonal changes in N2O emission that occur in natural estuaries are important to anticipate the future implications of global warming. This study showed the effect of temperature on denitrifying growth and nitrate reduction end product of Comamonas testoteroni isolated from estuarine sediment using both batch and continuous cultures. The μmax values of Comamonas testosteroni grown in anaerobic batch culture were increased with increasing temperature, and the highest μmax was found at 26 oC. Concentrations of nitrate reduced (mg-1 dried weight cells) were higher at low temperature. Concentrations of N2 produced were higher at low temperature and the production of N2 was higher than both NO2- and N2O productions

Screening of Lactic Acid Bacteria for the Purpose of Chitin Recovery Processing SISWA SETYAHADI
Microbiology Indonesia Vol. 1 No. 1 (2007): April 2007
Publisher : Indonesian Society for microbiology

Lactic acid fermentation has been studied as an alternative method of chitin recovery from the natural chitin compounds, such as shrimp waste. The purpose of this research was to identify a potential lactic acid bacterium which can produce large amount of lactic acid and has the ability to release chitin (demineralization) from shrimp-shell-waste. Among 11 bacteria tested, strain 15 was the strongest lactic acid producer yielding 1.09% (v/v) lactic acid in the media with a pH of 4.15 after 6 days incubation at 37 oC. After that, strains 17 and 23 produced 0.79 and 0.74% of lactic acid respectively. These three strains were selected for further experiments on various kinds of media using two-day incubation periods. No strains produced lactic acid well in MRS media containing lactose. The best medium for lactic acid production by strains 15 and 17 was MRS containing glucose, molasses or mixture of molasses and shrimp shell waste. Fermentation of shrimp shell waste using strain 15 caused an increase of viscosity reflecting an increase of soluble chitin in the media..

Ecological Approach to Unravel Streptomycete Diversity as an Unsurpassed Sources of Natural Bioactive Products LANGKAH SEMBIRING; MICHAEL GOODFELLOW
Microbiology Indonesia Vol. 2 No. 2 (2008): August 2008
Publisher : Indonesian Society for microbiology

Search and discovery for natural bioactive products have been so important to control the emergence of antibiotic resistant microbial pathogens. Therefore, novel microorganisms that produce such metabolites is extremely needed. The capacity of members of the genus Streptomyces to produce commercially significant bioactive metabolites, notably antibiotics remains unsurpassed. However, it is acknowledged that discovering commercially useful secondary metabolites from streptomycetes is becoming more difficult due to lack of knowledge on the ecology and complexity of streptomycete systematics. In fact, those are fundamental aspects for developing strategy and method for isolation. In order to devise an appropriate program for successful selective isolation of sreptomycetes, it is fundamentally important to understand their occurance and activity in nature. A multistep extraction procedure designed for representative sampling, called dispersion, and differential centrifugation technique in combination with the incorporation of antibiotics into isolation media has become one of the most important selective method for the isolation of streptomycetes from natural habitats. The availability of new procedures to selectively isolate representative of streptomycetes from natural habitats opens up the possibility to determine the extent of streptomycete diversity from various habitats. Hence, the capacity of well characterized streptomycete isolates to produce commercial novel active metabolites could be further assessed appropriately.

Ethnomedicinal Plant Terong Pungo (Solanum sp.) LINAWATI HARDJITO
Microbiology Indonesia Vol. 2 No. 2 (2008): August 2008
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5454/mi.2.2.2

The leaf of terong pungo Solanum sp. had been used traditionally in Aceh to treat toothache and relieve pain. Based on traditional knowledge, this research was conducted to examine the biological activities of its extract as an antibacterial agent and topoisomerase-I inhibitor. The topoisomerase-I inhibitor is one of the target molecules for anticancer compounds. In addition, it is also a target molecule for antimicrobial compounds. The antibacterial activity was examined using disc diffusion assay against Staphylococcus aureus and Escherichia coli isolated from medical patients. Topoisomerase-I inhibitor activity was determined using the method reported by TopoGEN, and its toxicity was examined against Artemia salina. Chloroform, ethyl acetate, and methanol treatments yielded crude extracts of 1.44, 1.20, and 2.69%, respectively. The chloroform extract exhibited neither antibacterial nor topoisomerase-I inhibitor activities, while the ethylacetate and methanol extracts at a concentration of 300 μg/paper disk positively inhibited the growth of both target bacteria or enzyme activity of topoisomerase-I at a concentration of 50 μg ml-1. The ethylacetate extract showed better activity against target bacteria and was chosen for further investigation. The results suggest that the crude extract showed antibacterial and topoisomerase-I inhibitor activity. Identification of the isolated active fraction indicated that it contained a steroidal alkaloid compound. This investigation revealed that the leaf of terong pungo contains biologically active compounds of pharmacological relevance.

Distribution of Clavibacter michiganensis subsp. michiganensis in Various Tomato Production Centers in Sumatra and Java APRIZAL ZAINAL; ASWALDI ANWAR; UJANG KHAIRUL; . SUDARSONO
Microbiology Indonesia Vol. 2 No. 2 (2008): August 2008
Publisher : Indonesian Society for microbiology

Bacterial canker, caused by Clavibacter michiganensis subsp. michiganensis is a newly introduced disease of tomatoes in Indonesia. Its existence was first officially reported in 2004. The objective of this research was to monitor the existence of C. michiganensis subsp. michiganensis in various tomato production centers in Sumatra and Java. Tomato samples showing symptoms of C. michiganensis subsp. michiganensis infection were collected from various tomato production centers in Sumatera and Java and the causal agents were isolated from these samples. Based on the occurrences of typical symptoms of C. michiganensis subsp. michiganensis infection in tomato, the incidence of suspected C. michiganensis subsp. Michiganensis infection ranged from 1-20%. From a total of 74 tomato plants sampled, 24 bacterial isolates exhibiting similar colony morphology to C. michiganensis subsp. michiganensis were obtained. After various physiological, hypersensitive response, and pathogenicity tests, 18 isolates derived from 14 tomato production centers in eight provinces in Sumatera and Java were identified as C. michiganensis subsp. michiganensis. Although the incidence was still low, results of these experiments further indicated that C. michiganensis subsp. michiganensis existed in Indonesia and had spread in a number of tomato production centers in Sumatra and Java. Positive results of C. michiganensis subsp. Michiganensis identification from suspected tomato samples should be a warning sign for all tomato production stakeholders in Indonesia, especially those responsible in regulating the seed trade, importation, and plant quarantine

A Supplement Based on Zn-Enriched Virgin Coconut Oil as an Antifungal agent for Vaginal Candidiasis Patients . HERY WINARSI; . HERNAYANTI; AGUS PURWANTO
Microbiology Indonesia Vol. 2 No. 2 (2008): August 2008
Publisher : Indonesian Society for microbiology

This research was conducted to investigate the amount of Candida albicans in vaginal secretion of Vaginal Candidiasis patients administered with Zn-enriched virgin coconut oil. Thirty respondents were selected based on several criteria as follows: the number of C. albicans colonies in the vaginal secretion was more than 105 cfu.ml-1, voluntary, healthy, willing to sign the informed consent and resided in Purwokerto. In Group A, 10 women were administered 2 tablespoons per day of Zn-enriched virgin coconut oil. In Group B, 10 women were administered 1 tablespoon per day of Zn-enriched virgin coconut oil; and in Group C, 10 women served as control group. Vaginal secretions were taken 3 times, before intervention (at baseline time), at 1 month and 2 months after intervention. Samples were taken by collecting vaginal secretions from the vaginal proximal region using a sterile cotton bud, which was then put into a tube containing sterile carrier media. The vaginal secretions were tested for the number of total C. albicans using Pour Plate Method. Two months after treatment, the number of colonies decreased from 4.4x106 to 2.5x106 cfu ml-1 (p=0.03) in Group A. There was no significant difference in the number of colonies between Group A and Group B, the number of C. albicans colonies was still above the normal range. Therefore, the recommended dosage of intervention with Zn-enriched virgin coconut oil is one tablespoon a day.

Optimum Concentration of Glucose and Orange II for Growth and Decolorization of Orange II by Enterococcus faecalis ID6017 under Static Culture V. IRENE MEITINIARTI; ENDANG S. SOETARTO; EKO SUGIHARTO; KRIS HERAWAN TIMOTIUS
Microbiology Indonesia Vol. 2 No. 2 (2008): August 2008
Publisher : Indonesian Society for microbiology

Growth and decolorization performance of bacterial grown on azodyes-containing-medium is influenced by various concentrations of carbon sources and azodyes. The optimum level of glucose and Orange II concentration for growth and Orange II decolorization by Enterococcus faecalis ID6017 are reported in this paper. The experiments were carried out in liquid static culture as batch experiments. Glucose and Orange II concentrations used in these experiments were 0.45, 0.90, 1.80 g l-1, and 40, 80, 120 mg l-1, respectively. The specific growth rate and decolorization rate of Orange II by E. faecalis were highest on the medium which contained at least 0.90 g l-1 glucose. It is necessary to note that glucose above 0.90 g l-1 gave no significant difference. On the medium containing 0.90 g l-1 glucose and 80 mg l-1 Orange II, E. faecalis grew with the highest specific growth rate (0.28 h-1) and Orange II decolorization rate (0.47 h-1). The maximum specific growth rate of biomass (μmax) and the halfsaturation coefficient (KS) under optimal conditions were 0.25 h-1 and 1.5 g.l-1, respectively. The kinetics of decolorization indicated that the process followed first order kinetics with respect to the initial concentration of Orange II. The inhibition constant (KI) was found to be 750 mg l-1 Orange II, indicating that Orange II concentration at e” 750 mg l-1 would inhibit bacterial growth to decolorize Orange II..

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Home Page

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Editorial Team

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Reviewer

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Contact Name Iman Rusmana

Contact Email rusmana13@yahoo.com

Phone +62217560536

Journal Mail Official microbiology.indonesia@gmail.com

Editorial Address kPERHIMPUNAN MIKROBIOLOGI INDONESIA (SeKretariat PERMI), Gedung 10.2 Indonesian Life Sciences Center (ILSC), Zona Bisnis Teknologi Puspiptek, Jalan Raya Serpong - Bogor Gunung Sindur, Jawa Barat 16340, Indonesia. Email: microbiology.indonesia@gmail.com

Location Kota tangerang, Banten INDONESIA

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Contact Name
Iman Rusmana

Contact Email
rusmana13@yahoo.com

Phone
+62217560536

Journal Mail Official
microbiology.indonesia@gmail.com

Editorial Address
kPERHIMPUNAN MIKROBIOLOGI INDONESIA (SeKretariat PERMI), Gedung 10.2 Indonesian Life Sciences Center (ILSC), Zona Bisnis Teknologi Puspiptek, Jalan Raya Serpong - Bogor Gunung Sindur, Jawa Barat 16340, Indonesia. Email: microbiology.indonesia@gmail.com

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Kota tangerang,

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