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BIOTROPIA - The Southeast Asian Journal of Tropical Biology
Published by Southeast Asian Regional Centre for Tropical Biology
ISSN : 02156334     EISSN : 1907770X     DOI : http://dx.doi.org/10.11598/btb
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary
BIOTROPIA, The Southeast Asian Journal of Tropical Biology, is a scientific publication of the Southeast Asian Ministers of Education Organization (SEAMEO) – Southeast Asian Regional Center for Tropical Biology (BIOTROP). It publishes articles in the broad areas of tropical ecosystems and environmental impacts, biodiversity conservation and sustainable development and biotechnology.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 4 Documents
 1 
Search results for , issue "No. 16 (2001)" : 4 Documents clear
INDUCTION OF SPAWNING IN THE TROPICAL WALKING CATFISH (Clarias batrachus) BY CONTROLLING WATER LEVEL AND TEMPERATURE M. ZAIRIN JR.; K. FURUKAWA; K. AiDA
BIOTROPIA - The Southeast Asian Journal of Tropical Biology No. 16 (2001)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (127.747 KB) | DOI: 10.11598/btb.2001.0.16.165

Abstract

This experiment was carried out to confirm  the role of water level  and water temperature in inducing the spawning of tropical walking catfish. Mature males and females reared under 23 - 25 °C, were paired and induced to spawn by controlling water level and water temperature. Decreasing water level and returning it to its original level resulted in a low spawning rate (less than 6.7 %) at 23 °C. Decreasing water  level with simultaneous increase in water temperature, followed  by returning the respective levels to their originals gave high spawning rates (41.7 — 50 %); whereas the same treatment but without any water temperature decreased when the water level was returned to the initial level, gave a low spawning rate (16.7 %). Increasing water level only, failed to induce spawning. A high spawning rate was obtained also when changes in water level were carried out under high temperature of 28 °C. No fish spawned in the absence of the environmental stimulation. From the results, it is confirmed that water level and temperature play important roles in inducing spawning of tropical walking catfish. Changes in water temperature probably increase the sensitivity of fish to the change in  water level. Prolonged  exposure to high  water temperature could  also improve the sensitivity of fish. Key words: Walking catfish / spawning / water level / water temperature
A NOVEL INTEGRON IN THE GENOME OF ESCHERICHIA COLI ISOLATED FROM INDONESIAN MONITOR LIZARD (VARANUS SPP). ESTI PUSPITASARI; ANTONIUS SUWANTO; AMARILA MALIK; WALTER ERDELEN
BIOTROPIA - The Southeast Asian Journal of Tropical Biology No. 16 (2001)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (97.942 KB) | DOI: 10.11598/btb.2001.0.16.163

Abstract

The genotype of antibiotic resistance  in natural isolates of Escherichia coli was determined  through integron detection and  characterization of the associated antibiotic  resistance. E. coli SG2 isolated from Varanus salvator  of Java demonstrated  resistance  to spectinomycin (50ng/ml)  and streptomycin (SOng/ml). Integron detection indicated  that eight isolates out of nine E. coli  isolates possessed a conserved segment of the integron. Amplification of  the inserted cassette of the integron in this SG2 isolate yielded a 1-kb DNA fragment. Sequence analyses indicated that this fragment was homologous with aad gene, which confirmed  the resistance  to spectinomycin/streptomycin. This is the first report on the presence of integron in the E. coli isolated from the environment. Key words: Integron / antibiotic resistance / Escherichia coli
CHARACTERIZATION OF MALAYSIAN WILD BANANAS BASED ON ANTHOCYANINS MUHAMMAD ASIF JAVED; MAK. CHAI; ROFINA YASMIN OTHMAN
BIOTROPIA - The Southeast Asian Journal of Tropical Biology No. 16 (2001)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2001.0.16.164

Abstract

The male buds of 16 Musa species (Musaceae) populations were investigated by HPLC for the occurrence of anthocyanins. The investigation was based on the presence of 6 anthocyanins. The 16 Musa samples could be classified into three distinct species i.e. Musa acuminata, Musa violascens and Musa balbisiana. Musa acuminata could be divided into two subspecies : malaccensis (lowland) and tmncata (highland) according to their constituents and content of major anthocyanins. No variation was observed in the composition of the anthocyanins of Kedah type ssp. siamea and Selangor types ssp. malaccensis. The classification of M. acuminata into two subspecies based on anthocyanin data further supported the current taxonomic grouping of the species. Key words: Musa acuminata/Musa violascens/Musa balbisiana/Musaceae /HPLC /chemotaxonomy
IN VITRO PROPAGATION OF ANGIOPTERIS EVECTA USING SPORES POONAM BHATIA; DAMIEN CUPITT; NANJAPPA ASHWATH
BIOTROPIA - The Southeast Asian Journal of Tropical Biology No. 16 (2001)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2001.0.16.161

Abstract

Techniques of establishing Angiopleris evecta plants  in vitro were studied. Soaking of A. evecta spores in water for 24 hours markedly reduced spore contamination. Soaking of the spores in 1 -2 % of sodium hypochlorite for  less  than 5 minutes allowed satisfactory disinfestation without affecting spore viability. Lower concentration of minerals (1/4 MS), presence of charcoal in the medium and exposure of the spores to light were crucial for spore germination and gainetophytc development of A. evecta. Keywords:   Angiopleris evecta I King fern / spore / MS medium / bleach / light / tissue culture / sporophyte / gamctophytc

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