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BIOTROPIA - The Southeast Asian Journal of Tropical Biology
ISSN : 02156334     EISSN : 1907770X     DOI : http://dx.doi.org/10.11598/btb
BIOTROPIA, The Southeast Asian Journal of Tropical Biology, is a scientific publication of the Southeast Asian Ministers of Education Organization (SEAMEO) – Southeast Asian Regional Center for Tropical Biology (BIOTROP). It publishes articles in the broad areas of tropical ecosystems and environmental impacts, biodiversity conservation and sustainable development and biotechnology.
Articles 5 Documents
Search results for , issue "No. 19 (2002)" : 5 Documents clear
FUNGAL POPULATION, AFLATOXIN AND FREE FATTY ACID CONTENTS OF PEANUTS PACKED IN DIFFERENT BAG TYPES BULAONG, SONIA S P
BIOTROPIA No. 19 (2002)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1371.393 KB) | DOI: 10.11598/btb.2002.0.19.229

Abstract

Shelled peanuts of Gajah var. with initial moisture content of 7% were stored at 11 kg/bag in four bag types namely: jute bag, polypropylene bag, jute bag doubled with thin polyethylene (PE), and jute bag doubled with thick PE. Storage was done for six months under warehouse conditions with monitoring of relative humidity and temperature. Samples taken at the beginning of storage and every month thereafter were analyzed for moisture content, fungal population, aflatoxin and free fatty acid contents. Statistical analyses showed that moisture content, fungal population, and free fatty acid contents were significantly higher in jute and polypropylene bags than in PE-dou,bled jute bags. No significant differences were obtained in aflatoxin contents among bag types but at the end of six months storage, toxin level in jute bag exceeded the 30 ppb limit. Polypropylene had second highest toxin level at 23 ppb. The PE-doubled bags had 17 and 19 ppb total aflatoxins for thin and thick films, respectively. The results indicated that the immediate packaging of dried shelled peanuts at safe moisture level in plastic films with water vapor transmission rated of 1 g/m2/24 hr or lower is recommended. This packaging will delay critical increases in moisture content, fungal population, aflatoxin and free fatty acid contents of peanut kernels at ambient storage conditions. Keywords: Peanuts / bag types / fungal population / aflatoxin content / free fatty acid content.
REVIEW ON AFLATOXIN IN INDONESIAN FOOD- AND FEEDSTUFFS AND THEIR PRODUCTS DHARMAPUTRA, OKKY SETYAWATI
BIOTROPIA No. 19 (2002)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1073.66 KB) | DOI: 10.11598/btb.2002.0.19.227

Abstract

 Aflatoxin is a human carcinogen that  could contaminate food- and feedstuffs, and hence is a major food quality problem throughout the world. Afiatoxin is produced by certain strains of AspergillusJlavus and //. parasiticus. A number of studies have been carried out in Indonesia on atlatoxin contamination in Indonesian food- and feedstuffs and their products from 1990 up to present. They were maize, maize product, peanuts, soybean and soybean meal, black and white pepper, feed ingredients; chicken and duck feeds. Samples were collected from farmers, traders (middlemen), retailers (markets), supermarkets, exporters; poultry and duck community-based farms; and feed mi l l  industries. High levels of aflatoxins were often found in maize, peanuts, chicken feed derived from markets, and duck feed. Low levels of aflatoxins were found in soybean meal and chicken feedstuff. Aflatoxins were not detected in soybean, black and white pepper. Other studies have also been carried out on the effect of carbondioxide (CO2), phosphine, black pepper extract and antagonistic fungi on aflatoxin production of  A. flavus in vitro\  and the effect of airtight storage, phosphine, ammonium hydroxide, fermentation process, bag types, and phosphine in combination with different bag types on atlatoxin contents of maize, peanuts and soybean meal. Some of these methods reduced aflatoxin contents significantly. Keywords: Aspergillus flavus I Aflatoxin / Food-and feed stuffs / Product
NOTES ON THE ASTERACEAE OF SUMATERA SRI SUDARMIYATI TJITROSOEDIRDJO
BIOTROPIA - The Southeast Asian Journal of Tropical Biology No. 19 (2002)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2002.0.19.230

Abstract

An account of the tribe composition, endemic taxa, comparison with adjacent areas and weedy Asteraceae of Sumatera is given. Based on the records of January 2000, there are 133 species of 74 genera in 11 tribes.  The tribe Heliantheae is the largest, with 28% of the total number of the genera, followed by Astereae with 15%, Inuleae 12%, Senecioneae 10%, Anthemideae, Eupatorieae and Lactuceae 8%, the other tribes are represented by 4% or less. The most diverse genus is Blumea with 14 species. Other genera are only represented by 10 species or less, usually 4, or 3, or 2, and mostly by 1 species only. Thirty nine or about 53% are exotic genera and the native ones are less than half of the total number of the genera. In terms of indigenous and endemic species, Sumatera is richer than Java. There are 1 genus, 7 species and 2 varieties of Asteraceae endemic to Sumatera. A number of 43 important weed species were introduced from Tropical America, Africa, Asia and Europe. Among these Chromolaena odorata and Mikania micrantha are reported as the most noxious ones.  List of the genera and species recorded in Sumatera is provided in this paper. Key words : Asteraceae/Sumatera/compositions/endemic species/distribution/weedy Asteraceae
MOLECULAR IDENTIFICATION OF EGG PARASITOID, TELENOMUS SPP. (HYMENOPTERA: SCELIONIDAE) FROM SEVERAL LOCATIONS IN JAVA USING RAPD-PCR YULIARTI, NETTI; HIDAYAT, PURNAMA; BUCHORI, DAMAYANTI
BIOTROPIA No. 19 (2002)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2002.0.19.226

Abstract

Random amplified polymorphic DNA (RAPD) amplified by the polymerase chain reaction (PCR) was used to determine the differences of four Telenomus species and five populations of T. rowani from several locations in Java. Amplification of genomic DNA by using primer P2 (Amersham Pharmacia Biotech) indicated that each Telenomus species had a unique set of RAPD bands. Two bands which characterized the genus are estimated to be 300 and 430 bp. Each species had three specific bright bands except T. dignoides which only had two specific bright bands. However, no bands are unique to any of the five populations of T. rowani and all of the bands are less than 500 base-pair. Cluster analysis using UPGMA (Unweighed Pair Group Method With Arithmatic Mean) showed that the four Telenomus species consist of two groups, T. rowani and T. remus  in one cluster and T. dignus with T. dignoides belonging to another cluster. Key words : PCR-RAPD / clustering / Telenomus spp.
PLOJDY ANALYSIS AND DNA CONTENT OF MUTANT BANAN "PISANG BERANGAN" USING FLOW CYTOMETRY OTHMAN, ROFINA YASMIN; SHADIA, ABDELGADIR RAYIS; CHAI, MAK
BIOTROPIA No. 19 (2002)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2002.0.19.228

Abstract

Mutagens cause random changes in the nuclear DNA or cytoplasmic organelles, resulting in gene, chromosomal or genomic mutations and hence, create variability. In this study, flow cytometry (FCM) was used to determine ploidy levels and DNA content in gamma-irradiated variants of mutated Pisang Berangan (cv. Intan, AAA) - a local banana genotype. Induced variants such as short plant stature (stunted growth), late flowering plants (late maturity) and abnormalities in bunch characters were selected to study possible changes at the DNA level. The study showed that DNA content of mutated plants differed from non-irradiated control and that irradiation had the most effect at high doses (40 and 60 Gy). The increase of DNA content in 20 Gy and 30 Gy treated plants was not more than that of the control plants. The values of genomic DNA content of gamma-irradiation variants decreased as the dose of irradiation increased from 20 to 60 Gy, indicating that the high dose of gamma-irradiation had a significant effect on the genome of the plants. The analysis further showed that phenotypic variation due to mutagenesis was reflected in the DNA content of the plants. The results also showed that ploidy levels were not affected by gamma-irradiation even at high doses. Keywords: Musa spp./mutation breeding/ flow cytometry/  ploidy level/ DNA content

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