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BIOTROPIA - The Southeast Asian Journal of Tropical Biology
ISSN : 02156334     EISSN : 1907770X     DOI : http://dx.doi.org/10.11598/btb
BIOTROPIA, The Southeast Asian Journal of Tropical Biology, is a scientific publication of the Southeast Asian Ministers of Education Organization (SEAMEO) – Southeast Asian Regional Center for Tropical Biology (BIOTROP). It publishes articles in the broad areas of tropical ecosystems and environmental impacts, biodiversity conservation and sustainable development and biotechnology.
Articles 6 Documents
Search results for , issue "No. 24 (2005)" : 6 Documents clear
EFFECT OF 3, 5, 3'-TRIIODOTHYRONINE (T3) HORMONE ON NUCLEIC ACID AND PROTEIN CONTENT OF THE MUSCLE AND THE GROWTH OF GIANT GOURAMY, OSPHRONEMUS GOURAMY LAC. SRI HANDAYANI; M. ZAIRIN Jr.; ING MOK.OGINTA; MARIA BINTANG
BIOTROPIA - The Southeast Asian Journal of Tropical Biology No. 24 (2005)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (366.793 KB) | DOI: 10.11598/btb.2005.0.24.175

Abstract

This experiment was conducted to study the effect of 3, 5, 3'-triiodothyroninc (Tj) hormone on nucleic acid and protein content of the muscle and the growth of giant gouramy, Osphronemus gouramy  Lac. Five experimental diets, which contain isocaloric diets, but different in T3 hormone level were used in this experiment (0.0, 2.5, 5.0, 7.5, and 10.0 mg T3 hormone/kg diet). The experimental diets were tested to three different groups offish for sixty days feeding trial. Fish body weight in groups I, II, and III were 0.39-0.42 g/fish; 19.11 -21.99 g/fish, and 37.52-40.79 g/fish, respectively. The results showed that the highest RNA, DNA concentration and RNA/DNA ratio of the muscle were produced by 10.0 mg T3 hormone/kg diet for group I and II; and 2.5 mg T3 hormone/kg diet for group III. Similar results also were found for the protein content of the whole body, protein retention, and the daily growth rate of the fish. Key words :   Osphronemus gouramy/Ti hormone/nucleic acid/protcin/growth
PHYTOTOXICITY AND FIELD EFFICACY OF EXSEROHILUM LONGIROSTRA JC/MIN THE CONTROL OF BARNYARDGRASS ECOTYPES (ECHINOCHLOA CRUS-GALLI VAR. CRUS-GALLI(L.) BEAUV) ABDUL SHUKOR JURAIMI; ARIFIN TASRIF; JUGAH KADIR; SUHAIMI NAPIS; SOETIKNO SLAMET SASTROUTOMO
BIOTROPIA - The Southeast Asian Journal of Tropical Biology No. 24 (2005)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (368.348 KB) | DOI: 10.11598/btb.2005.0.24.172

Abstract

Five selected ecotypes of bamyardgrass (Echinochloa crus-galli var. crus-gatti) from several rice growing areas in Malaysia and Indonesia were tested for their susceptibility  to the potential bioherbicide  (Exserohilum longirostratum). Bamyardgrass seedlings at the 2-3-lcaf stage were treated with 2.5xl07 conidia/ml from E. longirostratum  at different application frequencies (single, double and triple). In addition, aqueous extract assays were evaluated for the presence of a phytotoxic compound responsible for the virulence of the bioherbicide. Results of the study showed that disease severity significantly increased 20 days after treatment and resulted in mortality of the seedlings. Ecotypes from Perak and Lampung were most susceptible to the bioherbicide upon triple applications. Percentage dry weight reductions were 86.34 and 83.14%, respectively. Other ecotypes (Melaka, Banten and South Sulawesi) were observed to have a relatively similar response. Moreover, aqueous extracts of E. longirostratum  increased mortality up to 92.50% of bamyardgrass seedlings. These findings suggest that regular (double and triple) applications of E. longirostratum at a concentration of 2.5xl07 conidia/ml significantly increased mortality among bamyardgrass ecotypes. Mortality of the seedlings was attributed to the presence of a secondary phytotoxic metabolite. Key words :    Field efficacy / Phytotoxicity / Exserohilum longirostratum I Echinochloa crus-galli var. crusgalli I ecotypes.
EFFECT OF ANTANAN (CENTELLA ASIATICA) AND VITAMIN C ON THE BURSA OF FABRICIUS, LIVER MALONALDEHIDE AND PERFORMANCE OF HEAT-STRESSED BROILERS TOHA SUTARDI; ENGKUS KUSNADI; REVIANY WlDJAJAKUSUMA
BIOTROPIA - The Southeast Asian Journal of Tropical Biology No. 24 (2005)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (343.713 KB) | DOI: 10.11598/btb.2005.0.24.177

Abstract

High environmental temperatures may cause heat stress  in poultry. This may increase water consumption, decrease feed consumption and in rum, decrease productivity level. In addition, high temperature contributes to oxidative stress, a condition where oxidant activity (free radicals) exceeds antioxidant activity. In our research, antanan (Centelta asiatica)  and vitamin C were utilized as anti heat-stress agents for heat-stressed broilers. We used 120 male broilers 2 - 6  weeks old, kept at 31.98 ± 1.94 °C during the day and 27.36 ± 1.31 °C at night. The data collected were analyzed with a completely randomized factorial design of  2 x 3 (2 levels of vitamin C, 3 levels of antanan at 4 replications) and continued with the contrast-orthogonal test when significantly different. The results indicate that the treatments of 5 and 10% of antanan with or without 500 ppm of vitamin C and vitamin C alone significantly (P
MOLECULAR PHYLOGENETIC ANALYSIS OF MONASCUS FUNGI BASED ON INTERNAL TRANSCRIBED SPACER REGION N SUHARNA; Y. KIKUCHI; T. FUKATSU
BIOTROPIA - The Southeast Asian Journal of Tropical Biology No. 24 (2005)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (251.911 KB) | DOI: 10.11598/btb.2005.0.24.173

Abstract

A molecular analysis of internal transcribed spacer region has been carried out to reveal the relationship among 16 strains of Monascus spp. A primer set comprised primer ITS1 and ITS4 was used to amplify this region in which they were cloned and scqucnccd. We also compared the sequence result with M. purpureus AF458473, M.ruber AF458470, M. kaoliang AF451859, M. araneous AF458471 and M. pilosus AF451856 and one outgroup species Thermoascus crustaceus U18353. The result showed that 16 Monascus spp. were divided into two large clades while M. ruber AF458470 was basically separated from all those Monascus. One of the two large clades included the seven M. purpureus strains, M. purpureus AF458473, M. araneosus AF458471 and M. kaoliang AF451859. Another large cladc included the six Monascus sp. strains which typically have whitish colonies, the three M. ruber strains and M.pilosus AF451856. However, even outstanding morphological differences possessed by several white Monascus  and one whitish M. purpureus  strain, all Monascus  strains were suggested to be very closely related with similarity >99% almost 100%. Although this ITS analysis could not discriminate cultural and morphological differentiation of Monascus strains studied, yet there is still little genetic variation within these strains. Key words : Molecular genetics/Monascus spp./fungi
ANALYSIS OF THE TEMPERATURE DEPENDENCE OF CO2 ASSIMILATION RATE (STUDY CASE: GLYCINE MAXL. MERR) TANIA JUNE
BIOTROPIA - The Southeast Asian Journal of Tropical Biology No. 24 (2005)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2005.0.24.176

Abstract

The maximum rate of carboxylation (Kcmax) and maximum rate of regeneration of Ribulose bisphosphate (RuBP) (controlled by the rate of electron transport, Jmax) arc two processes governing the photosynthetic capacity of plants. Both processes are affected by temperature. This paper examines how the response of these two photosynthetic capacities to temperature determines the temperature response curve of the CO2-assimilation rate for plants grown at different temperatures, by using the concept of the Farquhar €3 photosynthesis model. The goal is to use photosynthetic parameters from CO2 and light curves to predict the temperature dependence of the CO2-assimilation rate (A) of soybean and to estimate the preferred growth temperature. Analysis shows that the optimum temperature of the assimilation rate changes with the changing temperature dependence of carboxylation and regeneration of RuBP. Key words :  temperature dependence/soybean/modeling photosynthesis/preferred growth temperature.
ASPERGILLUS FLA VUS INFECTION AND AFLATOXIN CONTAMINATION IN PEANUTS AT VARIOUS STAGES OF THE DELIVERY CHAINS IN CIANJUR REGENCY, WEST JAVA, INDONESIA OKKY S.DHARMAPUTRA
BIOTROPIA - The Southeast Asian Journal of Tropical Biology No. 24 (2005)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2005.0.24.174

Abstract

A survey to obtain information on pre- and postharvest handling of peanuts at farmer, collector, wholesaler and retailer levels, including  Aspergillus flavus  infection and aflatoxin  BI  contamination of peanuts collected in Cianjur regency, West Java, was conducted during the harvest period of the wet season of February 2004. The moisture contents and physical qualities of the peanuts were also determined. Thirteen and 40 dry pod samples were collected randomly from 12 farmers and 23 collectors, respectively. Seven dry kernel samples were also collected from collectors. Five and 45 dry kernel samples were collected randomly from 2 wholesalers and 45 retailers in traditional markets, respectively. Thus, a total of 110 dry peanut pod and kernel samples were collected. The results of interviews with farmers, collectors, wholesalers and retailers, and also the moisture contents and physical qualities of the peanuts arc described in this article. The percentages of samples infected by A. flavus were highest at the wholesaler as well as at retailer levels (100%, respectively), followed by those sampled at the  collectors (85.0 and 85.7%, respectively), and farmers (84.6%). The mean percentage of infected kernels in infected samples of peanuts collected from retailers was the highest (87.6%), followed by those collected from wholesalers (72.4%), collectors in the form of kernels (23.3%) and pods (17.7%), and farmers (15.2%). The range of aflatoxin  BI  contents in peanut samples collected from farmers (dry pods), collectors (dry pods), wholesalers (dry pods and kernels) and retailers (dry kernels) were < 3.6 -114.2, < 3.6 -2999.5 and < 3,6 - 34.1, < 3.6 - 6065.9, and < 3.6 - 6073.0 ppb, respectively. The highest aflatoxin B, contents at the wholesaler and retailer levels were 6065.9 ppb (in one sample) and 6073.0 ppb (in one sample), respectively. The percentage of samples contaminated with more than 15 ppb of aflatoxin BI was the highest in peanuts collected from wholesalers (80.0% of samples), followed by retailers (75.6%), farmers (38.5%) and collectors (30.0 and 14.3%). In 1999 Codex Alimcntarius Commission determined that the maximum total aflatoxin content in peanuts intended for further processing is 15 ppb, suggesting that an alarming proportion of peanuts throughout the Indonesian food chain arc in excess of this maximum limit. Key words : ,4,spergillius flavus/Aflatoxin/Peanuts/Cianjur regency

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